Gene description for IFT20
Gene name intraflagellar transport 20
Gene symbol IFT20
Other names/aliases -
Species Homo sapiens
 Database cross references - IFT20
ExoCarta ExoCarta_90410
Vesiclepedia VP_90410
Entrez Gene 90410
HGNC 30989
MIM 614394
UniProt Q8IY31  
 IFT20 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for IFT20
Molecular Function
    opsin binding GO:0002046 IEA
    protein binding GO:0005515 IPI
    small GTPase binding GO:0031267 IPI
Biological Process
    establishment of planar polarity GO:0001736 IEA
    kidney development GO:0001822 IEA
    smoothened signaling pathway GO:0007224 IEA
    spermatogenesis GO:0007283 ISS
    visual learning GO:0008542 IEA
    protein localization to Golgi apparatus GO:0034067 IMP
    intraciliary anterograde transport GO:0035720 NAS
    photoreceptor cell outer segment organization GO:0035845 IEA
    opsin transport GO:0036372 IEA
    establishment of epithelial cell apical/basal polarity GO:0045198 IEA
    positive regulation of cilium assembly GO:0045724 IMP
    centrosome localization GO:0051642 IEA
    cardiac muscle cell differentiation GO:0055007 IEA
    inner ear receptor cell stereocilium organization GO:0060122 IEA
    cilium assembly GO:0060271 IBA
    cilium assembly GO:0060271 IMP
    cilium assembly GO:0060271 IMP
    cilium assembly GO:0060271 NAS
    regulation of canonical Wnt signaling pathway GO:0060828 IEA
    neural precursor cell proliferation GO:0061351 IEA
    protein localization to cilium GO:0061512 IBA
    protein transmembrane transport GO:0071806 IEA
    protein localization to plasma membrane GO:0072659 IEA
    cochlea development GO:0090102 IEA
    regulation of cilium assembly GO:1902017 ISS
    regulation of platelet-derived growth factor receptor-alpha signaling pathway GO:2000583 ISS
    regulation of autophagosome assembly GO:2000785 ISS
Subcellular Localization
    Golgi membrane GO:0000139 TAS
    acrosomal vesicle GO:0001669 ISS
    photoreceptor outer segment GO:0001750 IEA
    manchette GO:0002177 ISS
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISS
    Golgi apparatus GO:0005794 ISS
    cis-Golgi network GO:0005801 IDA
    centrosome GO:0005813 IBA
    centrosome GO:0005813 IDA
    centriole GO:0005814 IEA
    microvillus GO:0005902 IEA
    cilium GO:0005929 ISS
    cilium GO:0005929 NAS
    cilium GO:0005929 TAS
    intraciliary transport particle GO:0030990 IBA
    intraciliary transport particle B GO:0030992 IPI
    intraciliary transport particle B GO:0030992 ISS
    motile cilium GO:0031514 IEA
    photoreceptor connecting cilium GO:0032391 IDA
    stereocilium GO:0032420 IEA
    ciliary basal body GO:0036064 IBA
    ciliary basal body GO:0036064 IDA
    neuron projection GO:0043005 IBA
    dendrite terminus GO:0044292 IEA
    ciliary tip GO:0097542 TAS
    ciliary base GO:0097546 IBA
    ciliary base GO:0097546 IDA
    non-motile cilium GO:0097730 IBA
    kinociliary basal body GO:1902636 IEA
 Experiment description of studies that identified IFT20 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for IFT20
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ATR  
Affinity Capture-MS Homo sapiens
2 VPS35 55737
Affinity Capture-MS Homo sapiens
3 CENPH  
Affinity Capture-MS Homo sapiens
4 BLOC1S6  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
5 SIKE1  
Affinity Capture-MS Homo sapiens
6 RABEP2 79874
Affinity Capture-MS Homo sapiens
7 MED29  
Affinity Capture-MS Homo sapiens
8 KIF20A 10112
Affinity Capture-MS Homo sapiens
9 OXNAD1 92106
Two-hybrid Homo sapiens
10 KPNA2 3838
Two-hybrid Homo sapiens
11 TTC30B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 VPS26A 9559
Affinity Capture-MS Homo sapiens
13 PBX4  
Two-hybrid Homo sapiens
14 GSTCD 79807
Affinity Capture-MS Homo sapiens
15 SH3BP5 9467
Two-hybrid Homo sapiens
16 EMILIN1 11117
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
17 IFT74 80173
Affinity Capture-MS Homo sapiens
18 PTPN12 5782
Two-hybrid Homo sapiens
19 IFT52 51098
Affinity Capture-MS Homo sapiens
20 IFT88 8100
Affinity Capture-MS Homo sapiens
21 COL4A3BP 10087
Affinity Capture-MS Homo sapiens
22 CCDC53 51019
Two-hybrid Homo sapiens
23 IFT81 28981
Affinity Capture-MS Homo sapiens
24 Atg16l1  
Co-localization Mus musculus
Affinity Capture-Western Mus musculus
25 XPO7 23039
Affinity Capture-MS Homo sapiens
26 UBE2Q1 55585
Affinity Capture-MS Homo sapiens
27 Kif23 71819
Affinity Capture-MS Mus musculus
28 ATG16L1 55054
Reconstituted Complex Homo sapiens
29 BET1 10282
Affinity Capture-MS Homo sapiens
30 FSD2 123722
Two-hybrid Homo sapiens
31 KNSTRN  
Two-hybrid Homo sapiens
32 EXOC7 23265
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
33 EBAG9 9166
Proximity Label-MS Homo sapiens
34 GNPAT 8443
Affinity Capture-MS Homo sapiens
35 HAUS4  
Affinity Capture-MS Homo sapiens
36 EXOC3 11336
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 MED4 29079
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
38 C1orf216  
Two-hybrid Homo sapiens
39 USP22 23326
Affinity Capture-MS Homo sapiens
40 SPAG5 10615
Affinity Capture-MS Homo sapiens
41 ZDHHC17 23390
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
42 AQP1 358
Two-hybrid Homo sapiens
43 TREX1  
Two-hybrid Homo sapiens
44 SYMPK 8189
Affinity Capture-MS Homo sapiens
45 IFT27 11020
Affinity Capture-MS Homo sapiens
46 WASF1 8936
Two-hybrid Homo sapiens
47 GOLGA2 2801
Two-hybrid Homo sapiens
48 HTT 3064
Two-hybrid Homo sapiens
49 ABI2 10152
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
50 DTNBP1  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
51 MKRN2 23609
Affinity Capture-RNA Homo sapiens
52 SCCPDH 51097
Affinity Capture-MS Homo sapiens
53 NUP62 23636
Two-hybrid Homo sapiens
54 CCDC51 79714
Affinity Capture-MS Homo sapiens
55 IFT172 26160
Affinity Capture-MS Homo sapiens
56 KIAA0391  
Affinity Capture-MS Homo sapiens
57 EXOC6B 23233
Affinity Capture-MS Homo sapiens
58 NME8  
Affinity Capture-MS Homo sapiens
59 VPS18 57617
Affinity Capture-MS Homo sapiens
60 CTNNAL1 8727
Affinity Capture-MS Homo sapiens
61 DISC1 27185
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
62 TTC30A 92104
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 ABI3 51225
Two-hybrid Homo sapiens
64 TTC26 79989
Affinity Capture-MS Homo sapiens
65 TARBP1 6894
Affinity Capture-MS Homo sapiens
66 TBCE 6905
Affinity Capture-MS Homo sapiens
67 TRAF3IP1 26146
Affinity Capture-MS Homo sapiens
68 UBE2W  
Affinity Capture-MS Homo sapiens
69 MIS18A  
Two-hybrid Homo sapiens
70 IFT57 55081
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
71 GPD2 2820
Affinity Capture-MS Homo sapiens
72 SYCE2  
Two-hybrid Homo sapiens
73 ATRIP  
Affinity Capture-MS Homo sapiens
74 HOMER1  
Affinity Capture-MS Homo sapiens
75 SERINC1 57515
Affinity Capture-MS Homo sapiens
76 OSBPL11 114885
Affinity Capture-MS Homo sapiens
77 ABTB2 25841
Affinity Capture-MS Homo sapiens
78 LIN9  
Affinity Capture-MS Homo sapiens
79 IFT46 56912
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
80 CLEC4M  
Two-hybrid Homo sapiens
81 USP24 23358
Affinity Capture-MS Homo sapiens
82 KAT5  
Two-hybrid Homo sapiens
83 NDC80 10403
Two-hybrid Homo sapiens
84 CORO1A 11151
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
85 DNAJC1 64215
Affinity Capture-MS Homo sapiens
86 KDM8  
Two-hybrid Homo sapiens
87 Exoc4  
Affinity Capture-Western Mus musculus
88 USHBP1  
Two-hybrid Homo sapiens
89 TKFC 26007
Affinity Capture-MS Homo sapiens
90 FBF1  
Two-hybrid Homo sapiens
91 FAM114A2  
Affinity Capture-MS Homo sapiens
92 LANCL2 55915
Affinity Capture-MS Homo sapiens
93 REEP6  
Affinity Capture-MS Homo sapiens
94 IFT22 64792
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
95 EXOC5 10640
Affinity Capture-MS Homo sapiens
96 SAPCD2  
Affinity Capture-MS Homo sapiens
97 BLOC1S2 282991
Affinity Capture-MS Homo sapiens
98 CES1P1  
Affinity Capture-MS Homo sapiens
99 NUP54 53371
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
100 RASSF4  
Two-hybrid Homo sapiens
101 HAUS1  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
102 CLUAP1 23059
Affinity Capture-MS Homo sapiens
103 DSTYK 25778
Affinity Capture-MS Homo sapiens
104 HSPB11 51668
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
105 CCHCR1  
Two-hybrid Homo sapiens
106 EPHX1 2052
Affinity Capture-MS Homo sapiens
107 PPP1R11  
Two-hybrid Homo sapiens
108 ARFIP2 23647
Affinity Capture-MS Homo sapiens
109 IFT80 57560
Affinity Capture-MS Homo sapiens
110 PSMA1 5682
Two-hybrid Homo sapiens
111 MED30  
Two-hybrid Homo sapiens
112 SPC25 57405
Affinity Capture-MS Homo sapiens
113 USP30  
Affinity Capture-MS Homo sapiens
114 FAM81B  
Two-hybrid Homo sapiens
115 KXD1 79036
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
116 HSDL1 83693
Affinity Capture-MS Homo sapiens
117 KRT13 3860
Two-hybrid Homo sapiens
118 CROCCP2  
Two-hybrid Homo sapiens
119 CCDC67  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
120 DYNC2H1 79659
Affinity Capture-MS Homo sapiens
121 PKN3 29941
Affinity Capture-MS Homo sapiens
122 C17orf59 54785
Proximity Label-MS Homo sapiens
View the network image/svg+xml



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