Gene description for IFT27
Gene name intraflagellar transport 27
Gene symbol IFT27
Other names/aliases BBS19
RABL4
RAYL
Species Homo sapiens
 Database cross references - IFT27
ExoCarta ExoCarta_11020
Vesiclepedia VP_11020
Entrez Gene 11020
HGNC 18626
MIM 615870
UniProt Q9BW83  
 IFT27 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocytes 26054723    
Mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for IFT27
Molecular Function
    GTPase activity GO:0003924 IBA
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IBA
Biological Process
    kidney development GO:0001822 ISS
    intracellular protein transport GO:0006886 IMP
    intracellular protein transport GO:0006886 ISS
    smoothened signaling pathway GO:0007224 ISS
    spermatogenesis GO:0007283 ISS
    vesicle-mediated transport GO:0016192 IBA
    intraciliary anterograde transport GO:0035720 NAS
    intraciliary transport GO:0042073 IBA
    intraciliary transport GO:0042073 IMP
    intraciliary transport GO:0042073 ISS
    inner ear receptor cell stereocilium organization GO:0060122 IEA
    cilium assembly GO:0060271 NAS
    cochlea development GO:0090102 IEA
Subcellular Localization
    Golgi membrane GO:0000139 IBA
    nucleus GO:0005634 IEA
    cytoplasm GO:0005737 ISS
    Golgi apparatus GO:0005794 IBA
    centrosome GO:0005813 IEA
    cilium GO:0005929 IBA
    cilium GO:0005929 IDA
    cilium GO:0005929 NAS
    cilium GO:0005929 TAS
    intraciliary transport particle B GO:0030992 IBA
    intraciliary transport particle B GO:0030992 IDA
    intraciliary transport particle B GO:0030992 IPI
    intraciliary transport particle B GO:0030992 ISS
    motile cilium GO:0031514 ISS
    sperm flagellum GO:0036126 ISS
    sperm midpiece GO:0097225 IEA
    sperm principal piece GO:0097228 IEA
    ciliary tip GO:0097542 TAS
 Experiment description of studies that identified IFT27 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
6
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for IFT27
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ATP2B3 492
Affinity Capture-MS Homo sapiens
2 TTC30B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 PRDX5 25824
Co-fractionation Homo sapiens
4 TTC30A 92104
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 HOOK1  
Proximity Label-MS Homo sapiens
6 ABCB6 10058
Affinity Capture-MS Homo sapiens
7 TTC26 79989
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 IFT20 90410
Affinity Capture-MS Homo sapiens
9 FAS 355
Affinity Capture-MS Homo sapiens
10 CLUAP1 23059
Affinity Capture-MS Homo sapiens
11 UBXN10  
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
12 GPR50  
Affinity Capture-MS Homo sapiens
13 TRAF3IP1 26146
Affinity Capture-MS Homo sapiens
14 HSPB11 51668
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
15 IFT57 55081
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 SMC6  
Affinity Capture-MS Homo sapiens
17 SETX  
Affinity Capture-MS Homo sapiens
18 HOOK3 84376
Proximity Label-MS Homo sapiens
19 SBF1 6305
Affinity Capture-MS Homo sapiens
20 RPP38 10557
Affinity Capture-MS Homo sapiens
21 IFT80 57560
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 MTMR4  
Affinity Capture-MS Homo sapiens
23 GOLGA2 2801
Two-hybrid Homo sapiens
24 B3GNT2 10678
Affinity Capture-MS Homo sapiens
25 IFT46 56912
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 IFT74 80173
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 IFT52 51098
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 IFT88 8100
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 RHBDD2  
Affinity Capture-MS Homo sapiens
30 IFT172 26160
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 BICD1 636
Proximity Label-MS Homo sapiens
32 ARL13B 200894
Affinity Capture-MS Homo sapiens
33 LCA5  
Affinity Capture-MS Homo sapiens
34 IFT81 28981
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 ATP7A 538
Affinity Capture-MS Homo sapiens
36 C2orf44  
Affinity Capture-MS Homo sapiens
37 IFT22 64792
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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