Gene description for CPSF6
Gene name cleavage and polyadenylation specific factor 6, 68kDa
Gene symbol CPSF6
Other names/aliases CFIM
CFIM68
HPBRII-4
HPBRII-7
Species Homo sapiens
 Database cross references - CPSF6
ExoCarta ExoCarta_11052
Entrez Gene 11052
HGNC 13871
MIM 604979
UniProt Q16630  
 CPSF6 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Hepatocytes 26054723    
Prostate cancer cells 25844599    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for CPSF6
Molecular Function
    mRNA binding GO:0003729 IDA
    protein binding GO:0005515 IPI
    poly(A) RNA binding GO:0044822 IDA
    nucleotide binding GO:0000166 IEA
    RNA binding GO:0003723 IDA
Biological Process
    mRNA polyadenylation GO:0006378 IMP
    mRNA processing GO:0006397 IDA
    protein tetramerization GO:0051262 IDA
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    paraspeckles GO:0042382 IDA
    membrane GO:0016020 IDA
    mRNA cleavage factor complex GO:0005849 IDA
    ribonucleoprotein complex GO:0030529 IDA
 Experiment description of studies that identified CPSF6 in exosomes
1
Experiment ID 236
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 237
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
3
Experiment ID 274
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|RAB5A
EV Cytosolic markers
✔
CD9|CD82|CD63|CD81
EV Membrane markers
✔
AIF
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T.
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel resistant
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Flow cytometry
Western blotting
4
Experiment ID 191
ISEV standards
✘ EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD81|CD9
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for CPSF6
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NCK2 8440
Two-hybrid Homo sapiens
2 FLJ20125  
Two-hybrid Homo sapiens
3 NUDT21 11051
Two-hybrid Homo sapiens
4 WWP2 11060
Two-hybrid Homo sapiens
5 RNPS1 10921
Affinity Capture-MS Homo sapiens
6 PLSCR1 5359
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
7 KLHL12  
Two-hybrid Homo sapiens
8 UBAC1 10422
Two-hybrid Homo sapiens
9 WWP1 11059
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
10 EWSR1 2130
Two-hybrid Homo sapiens
11 ARMC7  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which CPSF6 is involved
PathwayEvidenceSource
Signaling by FGFR1 fusion mutants TAS Reactome





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