Gene description for LEO1
Gene name Leo1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae)
Gene symbol LEO1
Other names/aliases RDL
Species Homo sapiens
 Database cross references - LEO1
ExoCarta ExoCarta_123169
Vesiclepedia VP_123169
Entrez Gene 123169
HGNC 30401
MIM 610507
UniProt Q8WVC0  
 LEO1 identified in sEVs derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
 Gene ontology annotations for LEO1
Molecular Function
    protein binding GO:0005515 IPI
    RNA polymerase II C-terminal domain phosphoserine binding GO:1990269 IBA
Biological Process
    endodermal cell fate commitment GO:0001711 ISS
    transcription elongation by RNA polymerase II GO:0006368 IDA
    Wnt signaling pathway GO:0016055 IEA
    stem cell population maintenance GO:0019827 IDA
    mRNA 3'-end processing GO:0031124 IMP
    positive regulation of transcription elongation by RNA polymerase II GO:0032968 IBA
    negative regulation of myeloid cell differentiation GO:0045638 IDA
    positive regulation of transcription by RNA polymerase II GO:0045944 IDA
Subcellular Localization
    fibrillar center GO:0001650 IDA
    nucleus GO:0005634 IBA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    centrosome GO:0005813 IDA
    Cdc73/Paf1 complex GO:0016593 IDA
 Experiment description of studies that identified LEO1 in sEVs
1
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for LEO1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GNL2 29889
Affinity Capture-MS Homo sapiens
2 UBE2H 7328
Affinity Capture-MS Homo sapiens
3 POLR2G 5436
Affinity Capture-MS Homo sapiens
4 HIST2H2BE 8349
Affinity Capture-MS Homo sapiens
5 COIL  
Proximity Label-MS Homo sapiens
6 ACTC1 70
Proximity Label-MS Homo sapiens
7 DBN1 1627
Cross-Linking-MS (XL-MS) Homo sapiens
8 NOP56 10528
Proximity Label-MS Homo sapiens
9 C9orf78 51759
Affinity Capture-MS Homo sapiens
10 CSNK2A1 1457
Co-fractionation Homo sapiens
Biochemical Activity Homo sapiens
11 SNRPF 6636
Affinity Capture-MS Homo sapiens
12 ANKRD28 23243
Co-fractionation Homo sapiens
13 CENPA  
Proximity Label-MS Homo sapiens
14 PPP1CC 5501
Affinity Capture-MS Homo sapiens
15 TCEB3 6924
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 DDX23 9416
Proximity Label-MS Homo sapiens
17 SYCE3  
Affinity Capture-MS Homo sapiens
18 CDK2AP1  
Affinity Capture-MS Homo sapiens
19 SNRPC 6631
Affinity Capture-MS Homo sapiens
20 ELOF1  
Affinity Capture-MS Homo sapiens
21 POLR1E  
Proximity Label-MS Homo sapiens
22 SYT2  
Affinity Capture-MS Homo sapiens
23 HADHA 3030
Affinity Capture-MS Homo sapiens
24 NIFK 84365
Proximity Label-MS Homo sapiens
25 POLR2C 5432
Affinity Capture-MS Homo sapiens
26 SERF2 10169
Affinity Capture-MS Homo sapiens
27 TULP2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 CSNK2B 1460
Affinity Capture-MS Homo sapiens
29 SLU7  
Affinity Capture-MS Homo sapiens
30 PPP1CA 5499
Affinity Capture-MS Homo sapiens
31 SRP9 6726
Affinity Capture-MS Homo sapiens
32 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
33 SUPT5H 6829
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
34 DNMT3A 1788
Affinity Capture-MS Homo sapiens
35 RTF1 23168
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
36 WDR61 80349
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 TCEA3  
Two-hybrid Homo sapiens
38 Wdr61  
Affinity Capture-MS Mus musculus
39 NTRK1 4914
Affinity Capture-MS Homo sapiens
40 HIST1H1E 3008
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 RAD18  
Affinity Capture-MS Homo sapiens
42 FLNB 2317
Co-fractionation Homo sapiens
43 HIST1H1C 3006
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
44 ACTB 60
Affinity Capture-MS Homo sapiens
45 CTR9 9646
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 COG5 10466
Affinity Capture-MS Homo sapiens
47 UBXN6 80700
Affinity Capture-MS Homo sapiens
48 CCNT1  
Co-fractionation Homo sapiens
49 CD3EAP  
Proximity Label-MS Homo sapiens
50 ANLN 54443
Affinity Capture-MS Homo sapiens
51 TAF7  
Co-fractionation Homo sapiens
52 PTPN11 5781
Affinity Capture-Western Homo sapiens
53 HADHB 3032
Affinity Capture-MS Homo sapiens
54 HIST1H3A 8350
Affinity Capture-Western Homo sapiens
55 KMT2A  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
56 CDC73  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 CIAO1 9391
Affinity Capture-MS Homo sapiens
58 XPO1 7514
Affinity Capture-MS Homo sapiens
59 SNRPB 6628
Affinity Capture-MS Homo sapiens
60 CTNNB1 1499
Affinity Capture-Western Homo sapiens
61 LMNB2 84823
Affinity Capture-MS Homo sapiens
62 RPRD2  
Affinity Capture-MS Homo sapiens
63 CPNE9  
Affinity Capture-MS Homo sapiens
64 UBE2E1  
Affinity Capture-Western Homo sapiens
65 DCTN3 11258
Co-fractionation Homo sapiens
66 FUS 2521
Affinity Capture-MS Homo sapiens
67 POLR2A 5430
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
68 AGO4  
Affinity Capture-MS Homo sapiens
69 DNMT3B  
Affinity Capture-MS Homo sapiens
70 ZC3H18  
Co-fractionation Homo sapiens
71 TOP1 7150
Affinity Capture-MS Homo sapiens
72 HIST1H1A 3024
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
73 TCEB2 6923
Affinity Capture-MS Homo sapiens
74 RPN1 6184
Cross-Linking-MS (XL-MS) Homo sapiens
75 PXK 54899
Affinity Capture-MS Homo sapiens
76 SUPT16H 11198
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
77 ARRB2 409
Affinity Capture-MS Homo sapiens
78 PARP1 142
Proximity Label-MS Homo sapiens
79 CPSF6 11052
Affinity Capture-MS Homo sapiens
80 BHLHB9 80823
Two-hybrid Homo sapiens
81 RUVBL2 10856
Affinity Capture-MS Homo sapiens
82 TCEA1 6917
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
83 SNRPA 6626
Affinity Capture-MS Homo sapiens
84 DCTN2 10540
Co-fractionation Homo sapiens
85 SETMAR  
Affinity Capture-MS Homo sapiens
86 SUPT4H1  
Co-fractionation Homo sapiens
87 CHD2 1106
Co-fractionation Homo sapiens
88 Rprd1b  
Affinity Capture-MS Mus musculus
89 AKR7A2 8574
Affinity Capture-MS Homo sapiens
90 PTGES3 10728
Affinity Capture-MS Homo sapiens
91 PAF1 54623
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
92 SSRP1 6749
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
93 CCT8L2  
Affinity Capture-MS Homo sapiens
94 CDK11A 728642
Co-fractionation Homo sapiens
95 TOP1MT  
Affinity Capture-MS Homo sapiens
96 EP300 2033
Affinity Capture-MS Homo sapiens
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