Gene description for NUP88
Gene name nucleoporin 88kDa
Gene symbol NUP88
Other names/aliases -
Species Homo sapiens
 Database cross references - NUP88
ExoCarta ExoCarta_4927
Vesiclepedia VP_4927
Entrez Gene 4927
HGNC 8067
MIM 602552
UniProt Q99567  
 NUP88 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for NUP88
Molecular Function
    protein binding GO:0005515 IPI
    structural constituent of nuclear pore GO:0017056 IEA
Biological Process
    ribosomal large subunit export from nucleus GO:0000055 IBA
    ribosomal small subunit export from nucleus GO:0000056 IBA
    mRNA export from nucleus GO:0006406 IBA
    protein import into nucleus GO:0006606 IBA
    nucleocytoplasmic transport GO:0006913 NAS
Subcellular Localization
    nuclear envelope GO:0005635 IDA
    nuclear pore GO:0005643 IBA
    nuclear pore GO:0005643 IDA
    nuclear pore GO:0005643 NAS
    nucleoplasm GO:0005654 IDA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified NUP88 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 211
MISEV standards
EM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
cytochrome c|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 212
MISEV standards
CEM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
Cytochrome C|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for NUP88
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SLFN11 91607
Affinity Capture-MS Homo sapiens
2 RIT1 6016
Negative Genetic Homo sapiens
3 MCM3 4172
Co-fractionation Homo sapiens
4 ETV6  
Proximity Label-MS Homo sapiens
5 AAK1 22848
Affinity Capture-MS Homo sapiens
6 Rcc1  
Affinity Capture-MS Mus musculus
7 NUP62 23636
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
8 KPNB1 3837
Affinity Capture-MS Homo sapiens
9 C9orf78 51759
Affinity Capture-MS Homo sapiens
10 C17orf59 54785
Proximity Label-MS Homo sapiens
11 CAND1 55832
Affinity Capture-MS Homo sapiens
12 TRIM62  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 C11orf30  
Affinity Capture-MS Homo sapiens
14 DYRK1A 1859
Affinity Capture-MS Homo sapiens
15 NUP153 9972
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
16 RANBP1 5902
Affinity Capture-MS Homo sapiens
17 AGTR1  
Two-hybrid Homo sapiens
18 ARFGEF2 10564
Co-fractionation Homo sapiens
19 MYCN  
Affinity Capture-MS Homo sapiens
20 RBM42  
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
21 KIF14 9928
Affinity Capture-MS Homo sapiens
22 NUP155 9631
Proximity Label-MS Homo sapiens
23 KRT8 3856
Proximity Label-MS Homo sapiens
24 Nup188  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
25 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
26 PARK2  
Affinity Capture-MS Homo sapiens
27 MAPRE1 22919
Affinity Capture-MS Homo sapiens
28 PPIP5K2 23262
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 Nup88  
Affinity Capture-MS Mus musculus
30 RAN 5901
Affinity Capture-MS Homo sapiens
31 NTRK1 4914
Affinity Capture-MS Homo sapiens
32 PEX14 5195
Proximity Label-MS Homo sapiens
33 XPOT 11260
Co-fractionation Homo sapiens
34 BICD2 23299
Proximity Label-MS Homo sapiens
35 Nup214  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
36 CUL2 8453
Affinity Capture-MS Homo sapiens
37 Ube2i  
Affinity Capture-MS Mus musculus
38 NUMA1 4926
Affinity Capture-MS Homo sapiens
39 CIRBP 1153
Co-fractionation Homo sapiens
40 Nup98  
Affinity Capture-MS Mus musculus
Reconstituted Complex Mus musculus
41 NUP62CL  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 TPR 7175
Co-fractionation Homo sapiens
43 SIRT7  
Affinity Capture-MS Homo sapiens
44 CUL3 8452
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 DES 1674
Affinity Capture-MS Homo sapiens
46 NUP43 348995
Proximity Label-MS Homo sapiens
47 NUP214 8021
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 SUZ12  
Affinity Capture-MS Homo sapiens
49 NXF1 10482
Affinity Capture-MS Homo sapiens
50 XPO1 7514
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 COG6 57511
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 DNAJC24  
Affinity Capture-MS Homo sapiens
53 Nup107  
Affinity Capture-MS Mus musculus
54 RPA3 6119
Proximity Label-MS Homo sapiens
55 FDPS 2224
Affinity Capture-MS Homo sapiens
56 SET 6418
Affinity Capture-Western Homo sapiens
57 RNF2  
Affinity Capture-MS Homo sapiens
58 SEH1L 81929
Affinity Capture-MS Homo sapiens
59 CCDC88A 55704
Affinity Capture-MS Homo sapiens
60 KRT19 3880
Proximity Label-MS Homo sapiens
61 RAE1 8480
Affinity Capture-MS Homo sapiens
62 ACBD5 91452
Proximity Label-MS Homo sapiens
63 SEC24B 10427
Affinity Capture-MS Homo sapiens
64 PDZD9  
Affinity Capture-MS Homo sapiens
65 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
66 NUTF2 10204
Affinity Capture-MS Homo sapiens
67 SIRT6  
Affinity Capture-MS Homo sapiens
68 CPSF6 11052
Co-fractionation Homo sapiens
69 PLEC 5339
Affinity Capture-MS Homo sapiens
70 MEN1 4221
Affinity Capture-MS Homo sapiens
71 G3BP1 10146
Affinity Capture-MS Homo sapiens
72 DCTN2 10540
Proximity Label-MS Homo sapiens
73 G3BP2 9908
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
74 NUP35 129401
Proximity Label-MS Homo sapiens
75 DDX19A 55308
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 NUP98 4928
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
77 Obfc1  
Affinity Capture-MS Mus musculus
78 ANAPC2 29882
Proximity Label-MS Homo sapiens
79 NUP107 57122
Affinity Capture-MS Homo sapiens
80 TRIM37  
Proximity Label-MS Homo sapiens
81 PMVK 10654
Affinity Capture-MS Homo sapiens
82 PXN 5829
Proximity Label-MS Homo sapiens
83 CLTA 1211
Proximity Label-MS Homo sapiens
84 CIT 11113
Affinity Capture-MS Homo sapiens
85 Ranbp2  
Affinity Capture-MS Mus musculus
86 FLNC 2318
Co-fractionation Homo sapiens
87 SERBP1 26135
Affinity Capture-MS Homo sapiens
88 KRAS 3845
Negative Genetic Homo sapiens
89 MYC  
Affinity Capture-MS Homo sapiens
90 RANBP2 5903
Co-fractionation Homo sapiens
View the network image/svg+xml
 Pathways in which NUP88 is involved
PathwayEvidenceSource
Antiviral mechanism by IFN-stimulated genes TAS Reactome
Cell Cycle TAS Reactome
Cell Cycle, Mitotic TAS Reactome
Cellular response to heat stress TAS Reactome
Cellular responses to stimuli TAS Reactome
Cellular responses to stress TAS Reactome
Cytokine Signaling in Immune system TAS Reactome
Defective TPR may confer susceptibility towards thyroid papillary carcinoma (TPC) TAS Reactome
Disease TAS Reactome
Disease IEA Reactome
Disorders of transmembrane transporters TAS Reactome
Export of Viral Ribonucleoproteins from Nucleus TAS Reactome
Gene expression (Transcription) TAS Reactome
Gene Silencing by RNA TAS Reactome
Glucose metabolism TAS Reactome
Glycolysis TAS Reactome
HCMV Early Events TAS Reactome
HCMV Infection TAS Reactome
HCMV Late Events TAS Reactome
HIV Infection IEA Reactome
HIV Infection TAS Reactome
HIV Life Cycle TAS Reactome
Host Interactions of HIV factors IEA Reactome
Host Interactions of HIV factors TAS Reactome
Immune System TAS Reactome
Infectious disease TAS Reactome
Infectious disease IEA Reactome
Influenza Infection TAS Reactome
Influenza Viral RNA Transcription and Replication TAS Reactome
Interactions of Rev with host cellular proteins IEA Reactome
Interactions of Rev with host cellular proteins TAS Reactome
Interactions of Vpr with host cellular proteins TAS Reactome
Interferon Signaling TAS Reactome
ISG15 antiviral mechanism TAS Reactome
Late Phase of HIV Life Cycle TAS Reactome
M Phase TAS Reactome
Metabolism TAS Reactome
Metabolism of carbohydrates TAS Reactome
Metabolism of non-coding RNA TAS Reactome
Metabolism of proteins TAS Reactome
Metabolism of proteins IEA Reactome
Metabolism of RNA TAS Reactome
Mitotic Prophase TAS Reactome
NEP/NS2 Interacts with the Cellular Export Machinery TAS Reactome
NS1 Mediated Effects on Host Pathways TAS Reactome
Nuclear Envelope Breakdown TAS Reactome
Nuclear import of Rev protein IEA Reactome
Nuclear Pore Complex (NPC) Disassembly TAS Reactome
Post-translational protein modification TAS Reactome
Post-translational protein modification IEA Reactome
Processing of Capped Intron-Containing Pre-mRNA TAS Reactome
Regulation of Glucokinase by Glucokinase Regulatory Protein TAS Reactome
Regulation of HSF1-mediated heat shock response TAS Reactome
Rev-mediated nuclear export of HIV RNA TAS Reactome
SARS-CoV Infections TAS Reactome
SARS-CoV-2 activates/modulates innate and adaptive immune responses TAS Reactome
SARS-CoV-2 Infection TAS Reactome
SARS-CoV-2-host interactions TAS Reactome
SLC transporter disorders TAS Reactome
snRNP Assembly TAS Reactome
SUMO E3 ligases SUMOylate target proteins TAS Reactome
SUMO E3 ligases SUMOylate target proteins IEA Reactome
SUMOylation TAS Reactome
SUMOylation IEA Reactome
SUMOylation of chromatin organization proteins TAS Reactome
SUMOylation of DNA damage response and repair proteins TAS Reactome
SUMOylation of DNA replication proteins IEA Reactome
SUMOylation of DNA replication proteins TAS Reactome
SUMOylation of RNA binding proteins TAS Reactome
SUMOylation of SUMOylation proteins TAS Reactome
SUMOylation of ubiquitinylation proteins TAS Reactome
Transcriptional regulation by small RNAs TAS Reactome
Transport of Mature mRNA derived from an Intron-Containing Transcript TAS Reactome
Transport of Mature mRNA Derived from an Intronless Transcript TAS Reactome
Transport of Mature mRNAs Derived from Intronless Transcripts TAS Reactome
Transport of Mature Transcript to Cytoplasm TAS Reactome
Transport of Ribonucleoproteins into the Host Nucleus TAS Reactome
Transport of the SLBP Dependant Mature mRNA TAS Reactome
Transport of the SLBP independent Mature mRNA TAS Reactome
tRNA processing TAS Reactome
tRNA processing in the nucleus TAS Reactome
Viral Infection Pathways TAS Reactome
Viral Infection Pathways IEA Reactome
Viral Messenger RNA Synthesis TAS Reactome
Vpr-mediated nuclear import of PICs TAS Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here