Gene description for PLSCR1
Gene name phospholipid scramblase 1
Gene symbol PLSCR1
Other names/aliases MMTRA1B
Species Homo sapiens
 Database cross references - PLSCR1
ExoCarta ExoCarta_5359
Vesiclepedia VP_5359
Entrez Gene 5359
HGNC 9092
MIM 604170
UniProt O15162  
 PLSCR1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 34887515    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Pancreatic duct fluid 29339034    
Pancreatic duct fluid 29339034    
Pancreatic duct fluid 29339034    
Prostate cancer cells 25844599    
Prostate cancer cells 25844599    
Squamous carcinoma cells 20124223    
Urine 15326289    
Urine 19056867    
Urine 22418980    
Urine 22418980    
 Gene ontology annotations for PLSCR1
Molecular Function
    magnesium ion binding GO:0000287 IDA
    DNA-binding transcription activator activity, RNA polymerase II-specific GO:0001228 IDA
    virus receptor activity GO:0001618 IMP
    DNA binding GO:0003677 IEA
    nuclease activity GO:0004518 IMP
    epidermal growth factor receptor binding GO:0005154 IPI
    calcium ion binding GO:0005509 IDA
    calcium ion binding GO:0005509 NAS
    protein binding GO:0005515 IPI
    zinc ion binding GO:0008270 IDA
    SH3 domain binding GO:0017124 IDA
    phospholipid scramblase activity GO:0017128 IBA
    phospholipid scramblase activity GO:0017128 IDA
    enzyme binding GO:0019899 IPI
    lead ion binding GO:0032791 IDA
    CD4 receptor binding GO:0042609 IPI
    mercury ion binding GO:0045340 IDA
Biological Process
    phosphatidylserine biosynthetic process GO:0006659 ISS
    apoptotic process GO:0006915 IDA
    acute-phase response GO:0006953 ISS
    response to lead ion GO:0010288 IDA
    positive regulation of gene expression GO:0010628 IMP
    plasma membrane phospholipid scrambling GO:0017121 IBA
    plasma membrane phospholipid scrambling GO:0017121 IDA
    platelet activation GO:0030168 NAS
    regulation of mast cell activation GO:0033003 ISS
    response to interferon-beta GO:0035456 IMP
    negative regulation of viral genome replication GO:0045071 IMP
    positive regulation of innate immune response GO:0045089 IMP
    positive regulation of transcription by RNA polymerase II GO:0045944 IDA
    symbiont entry into host cell GO:0046718 IEA
    negative regulation of phagocytosis GO:0050765 IMP
    defense response to virus GO:0051607 IMP
    regulation of Fc receptor mediated stimulatory signaling pathway GO:0060368 ISS
    phosphatidylserine exposure on apoptotic cell surface GO:0070782 IMP
    positive regulation of chromosome separation GO:1905820 IDA
    positive regulation of DNA topoisomerase (ATP-hydrolyzing) activity GO:2000373 IDA
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    nucleolus GO:0005730 IDA
    cytoplasm GO:0005737 IDA
    Golgi apparatus GO:0005794 IDA
    cytosol GO:0005829 IDA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    membrane GO:0016020 HDA
    membrane raft GO:0045121 IDA
    perinuclear region of cytoplasm GO:0048471 IDA
    collagen-containing extracellular matrix GO:0062023 IDA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified PLSCR1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
11
Experiment ID 254
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name MNT-1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
12
Experiment ID 255
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name G1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
13
Experiment ID 256
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 501mel
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
14
Experiment ID 257
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name Daju
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
15
Experiment ID 258
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name SKMEL28
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
16
Experiment ID 260
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 1205Lu
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
17
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
18
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
19
Experiment ID 622
MISEV standards
✔
EM
Biophysical techniques
Enriched markers
✔
GOLGA2|CYC1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 29339034    
Organism Homo sapiens
Experiment description Extracellular matrix proteins and carcinoembryonic antigen-related cell adhesion molecules characterize pancreatic duct fluid exosomes in patients with pancreatic cancer
Authors "Zheng J, Hernandez JM, Doussot A, Bojmar L, Zambirinis CP, Costa-Silva B, van Beek EJAH, Mark MT, Molina H, Askan G, Basturk O, Gonen M, Kingham TP, Allen PJ, D'Angelica MI, DeMatteo RP, Lyden D, Jarnagin WR."
Journal name HPB
Publication year 2018
Sample Pancreatic duct fluid
Sample name From patients with Pancreatic ductal adenocarcinoma
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
20
Experiment ID 623
MISEV standards
✘ Biophysical techniques
Enriched markers
✔
GOLGA2|CYC1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 29339034    
Organism Homo sapiens
Experiment description Extracellular matrix proteins and carcinoembryonic antigen-related cell adhesion molecules characterize pancreatic duct fluid exosomes in patients with pancreatic cancer
Authors "Zheng J, Hernandez JM, Doussot A, Bojmar L, Zambirinis CP, Costa-Silva B, van Beek EJAH, Mark MT, Molina H, Askan G, Basturk O, Gonen M, Kingham TP, Allen PJ, D'Angelica MI, DeMatteo RP, Lyden D, Jarnagin WR."
Journal name HPB
Publication year 2018
Sample Pancreatic duct fluid
Sample name From patients with IPMN with microinvasive cancer (IPMNca)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
21
Experiment ID 625
MISEV standards
✘ Biophysical techniques
Enriched markers
✔
GOLGA2|CYC1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 29339034    
Organism Homo sapiens
Experiment description Extracellular matrix proteins and carcinoembryonic antigen-related cell adhesion molecules characterize pancreatic duct fluid exosomes in patients with pancreatic cancer
Authors "Zheng J, Hernandez JM, Doussot A, Bojmar L, Zambirinis CP, Costa-Silva B, van Beek EJAH, Mark MT, Molina H, Askan G, Basturk O, Gonen M, Kingham TP, Allen PJ, D'Angelica MI, DeMatteo RP, Lyden D, Jarnagin WR."
Journal name HPB
Publication year 2018
Sample Pancreatic duct fluid
Sample name From patients with benign pancreatic disease
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
22
Experiment ID 275
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
23
Experiment ID 274
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel resistant
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Flow cytometry
Western blotting
24
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔
Alix|CD81|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
25
Experiment ID 13
MISEV standards
✔
IEM
Biophysical techniques
✔
Alix|RAB4|RAB5B|RAB11|TSG101|CD9|AQP2|AQP1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15326289    
Organism Homo sapiens
Experiment description Identification and proteomic profiling of exosomes in human urine.
Authors "Pisitkun T, Shen RF, Knepper MA"
Journal name PNAS
Publication year 2004
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
26
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
27
Experiment ID 196
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal high density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
28
Experiment ID 197
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal low density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PLSCR1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MVP 9961
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
2 TM4SF5  
Affinity Capture-MS Homo sapiens
3 PCED1A  
Two-hybrid Homo sapiens
4 ENKD1  
Two-hybrid Homo sapiens
5 SLC31A2  
Affinity Capture-MS Homo sapiens
6 PRR13  
Two-hybrid Homo sapiens
7 KRTAP10-11  
Two-hybrid Homo sapiens
8 SHC1 6464
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
9 KRTCAP2 200185
Affinity Capture-MS Homo sapiens
10 FRS3 10817
Two-hybrid Homo sapiens
11 LGALS9C  
Two-hybrid Homo sapiens
12 MFNG  
Affinity Capture-MS Homo sapiens
13 ARL6IP5 10550
Affinity Capture-MS Homo sapiens
14 DLK2  
Two-hybrid Homo sapiens
15 CRK 1398
Two-hybrid Homo sapiens
16 HOXA9  
Two-hybrid Homo sapiens
17 MGAT5B  
Two-hybrid Homo sapiens
18 PLSCR4  
Two-hybrid Homo sapiens
19 SLC2A1 6513
Affinity Capture-MS Homo sapiens
20 FAM168A  
Affinity Capture-MS Homo sapiens
21 RASD1  
Two-hybrid Homo sapiens
22 ATN1  
Two-hybrid Homo sapiens
23 FFAR1  
Affinity Capture-MS Homo sapiens
24 VASP 7408
Two-hybrid Homo sapiens
25 SLPI 6590
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
26 SPG7  
Two-hybrid Homo sapiens
27 HOXB6 3216
Two-hybrid Homo sapiens
28 KRTAP4-12  
Two-hybrid Homo sapiens
29 OGDH 4967
Two-hybrid Homo sapiens
30 PKD2 5311
Two-hybrid Homo sapiens
31 LCE3C  
Two-hybrid Homo sapiens
32 DMRT3  
Two-hybrid Homo sapiens
33 SMCP  
Two-hybrid Homo sapiens
34 ZNF638 27332
Two-hybrid Homo sapiens
35 FAM8A1 51439
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 LASP1 3927
Two-hybrid Homo sapiens
37 C10orf54 64115
Two-hybrid Homo sapiens
38 SRC 6714
Biochemical Activity Homo sapiens
39 LCE2D  
Two-hybrid Homo sapiens
40 YIPF3 25844
Two-hybrid Homo sapiens
41 SIGMAR1 10280
Affinity Capture-MS Homo sapiens
42 KRTAP4-2  
Two-hybrid Homo sapiens
43 LINC01547  
Two-hybrid Homo sapiens
44 EWSR1 2130
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
45 CTSZ 1522
Two-hybrid Homo sapiens
46 RTN1 6252
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 KRTAP4-11  
Two-hybrid Homo sapiens
48 CATSPER1  
Two-hybrid Homo sapiens
49 DEF6 50619
Two-hybrid Homo sapiens
50 SLC35A2  
Two-hybrid Homo sapiens
51 ARNT2  
Two-hybrid Homo sapiens
52 NEDD4 4734
Affinity Capture-MS Homo sapiens
53 GPR17 2840
Affinity Capture-MS Homo sapiens
54 KRTAP5-6  
Two-hybrid Homo sapiens
55 ADAMTSL4  
Two-hybrid Homo sapiens
56 LONRF1  
Two-hybrid Homo sapiens
57 HRG 3273
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
58 CRY1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
59 FBLN1 2192
Two-hybrid Homo sapiens
60 ZBTB16  
Two-hybrid Homo sapiens
61 CCDC33 80125
Two-hybrid Homo sapiens
62 TRIM42  
Two-hybrid Homo sapiens
63 SCNM1  
Two-hybrid Homo sapiens
64 HEY2  
Two-hybrid Homo sapiens
65 ZNF417  
Two-hybrid Homo sapiens
66 DAZAP2  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
67 NECAP2 55707
Two-hybrid Homo sapiens
68 C12orf49  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 RGS3 5998
Two-hybrid Homo sapiens
70 KIAA1683  
Two-hybrid Homo sapiens
71 CDH1 999
Proximity Label-MS Homo sapiens
72 CLCC1 23155
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
73 GDPD5 81544
Two-hybrid Homo sapiens
74 CRKL 1399
Two-hybrid Homo sapiens
75 NPDC1  
Two-hybrid Homo sapiens
76 NOC4L 79050
Two-hybrid Homo sapiens
77 DTX2 113878
Two-hybrid Homo sapiens
78 THBS1 7057
Two-hybrid Homo sapiens
79 FAM103A1  
Two-hybrid Homo sapiens
80 P2RY6  
Two-hybrid Homo sapiens
81 ZNF581  
Two-hybrid Homo sapiens
82 GNAI2 2771
Two-hybrid Homo sapiens
83 CD63 967
Affinity Capture-MS Homo sapiens
84 CDC42EP1 11135
Two-hybrid Homo sapiens
85 ADCY7 113
Two-hybrid Homo sapiens
86 MED15  
Two-hybrid Homo sapiens
87 PHLDA1 22822
Two-hybrid Homo sapiens
88 RBL1  
Two-hybrid Homo sapiens
89 REEP5 7905
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
90 IP6K2  
Two-hybrid Homo sapiens
91 EXD3  
Two-hybrid Homo sapiens
92 GPRIN2  
Two-hybrid Homo sapiens
93 PGLS 25796
Two-hybrid Homo sapiens
94 RERE 473
Two-hybrid Homo sapiens
95 NR0B2  
Two-hybrid Homo sapiens
96 NEU4  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
97 ATG12  
Two-hybrid Homo sapiens
98 TMEM128  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
99 SF1 7536
Two-hybrid Homo sapiens
100 EGFR 1956
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
101 BOLA1 51027
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
102 HOXA1 3198
Two-hybrid Homo sapiens
103 SPATA8  
Two-hybrid Homo sapiens
104 TFG 10342
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
105 C10orf62  
Two-hybrid Homo sapiens
106 C10orf10  
Two-hybrid Homo sapiens
107 Hoxa1  
Two-hybrid Mus musculus
Affinity Capture-Western Mus musculus
108 MAPK6  
Two-hybrid Homo sapiens
109 FAM134C 162427
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
110 CNR2  
Affinity Capture-MS Homo sapiens
111 CHRD  
Two-hybrid Homo sapiens
112 CMTM5  
Affinity Capture-MS Homo sapiens
113 BCL6B  
Two-hybrid Homo sapiens
114 KRTAP10-3  
Two-hybrid Homo sapiens
115 FRAT1 10023
Two-hybrid Homo sapiens
116 PPDPF  
Two-hybrid Homo sapiens
117 LINC00663  
Two-hybrid Homo sapiens
118 ZNF587  
Two-hybrid Homo sapiens
119 KRTAP9-2  
Two-hybrid Homo sapiens
120 LCE4A  
Two-hybrid Homo sapiens
121 PML 5371
Two-hybrid Homo sapiens
122 CPSF3L 54973
Two-hybrid Homo sapiens
123 SPRY2 10253
Two-hybrid Homo sapiens
124 C1orf95  
Affinity Capture-MS Homo sapiens
125 REEP6  
Affinity Capture-MS Homo sapiens
126 NTN4 59277
Two-hybrid Homo sapiens
127 DDIAS  
Two-hybrid Homo sapiens
128 APP 351
Reconstituted Complex Homo sapiens
129 KIF1A 547
Two-hybrid Homo sapiens
130 ESR2  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
131 STK16 8576
Two-hybrid Homo sapiens
132 TACR1  
Affinity Capture-MS Homo sapiens
133 DOCK2 1794
Two-hybrid Homo sapiens
134 SLC25A6 293
Two-hybrid Homo sapiens
135 PDE2A  
Affinity Capture-MS Homo sapiens
136 ILF3 3609
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
137 CPSF6 11052
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
138 VPS37C 55048
Two-hybrid Homo sapiens
139 CCER1  
Two-hybrid Homo sapiens
140 SMARCC1 6599
Two-hybrid Homo sapiens
141 APEX1 328
Affinity Capture-RNA Homo sapiens
142 PITX1  
Two-hybrid Homo sapiens
143 DHRS1 115817
Two-hybrid Homo sapiens
144 ZNF764 92595
Two-hybrid Homo sapiens
145 PLSCR3 57048
Two-hybrid Homo sapiens
146 ANXA11 311
Two-hybrid Homo sapiens
147 GDE1 51573
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
148 REEP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
149 CTBP1-AS2  
Two-hybrid Homo sapiens
150 DIO3  
Affinity Capture-MS Homo sapiens
151 FBXL18 80028
Two-hybrid Homo sapiens
152 GLRX3 10539
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
153 CNTFR 1271
Two-hybrid Homo sapiens
154 EP300 2033
Two-hybrid Homo sapiens
155 EFEMP2 30008
Two-hybrid Homo sapiens
156 KRTAP10-9  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which PLSCR1 is involved
No pathways found





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