Gene description for CSTF3
Gene name cleavage stimulation factor, 3' pre-RNA, subunit 3, 77kDa
Gene symbol CSTF3
Other names/aliases CSTF-77
Species Homo sapiens
 Database cross references - CSTF3
ExoCarta ExoCarta_1479
Vesiclepedia VP_1479
Entrez Gene 1479
HGNC 2485
MIM 600367
UniProt Q12996  
 CSTF3 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
Mesenchymal stem cells 36408942    
Neuroblastoma cells 25944692    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for CSTF3
Molecular Function
    RNA binding GO:0003723 HDA
    mRNA binding GO:0003729 IBA
    protein binding GO:0005515 IPI
Biological Process
    RNA 3'-end processing GO:0031123 IBA
    co-transcriptional mRNA 3'-end processing, cleavage and polyadenylation pathway GO:0180010 IDA
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    mRNA cleavage stimulating factor complex GO:0005848 IDA
 Experiment description of studies that identified CSTF3 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
9
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for CSTF3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MACROD1 28992
Affinity Capture-MS Homo sapiens
2 ATG16L1 55054
Affinity Capture-MS Homo sapiens
3 SCARNA22  
Affinity Capture-RNA Homo sapiens
4 KLHL20  
Affinity Capture-MS Homo sapiens
5 RBBP6 5930
Affinity Capture-Western Homo sapiens
6 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
7 AHSA1 10598
Co-fractionation Homo sapiens
8 CPSF1 29894
Reconstituted Complex Homo sapiens
9 FEM1A  
Affinity Capture-MS Homo sapiens
10 CSTF1 1477
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
11 Myh9 17886
Affinity Capture-MS Mus musculus
12 HDDC3  
Affinity Capture-MS Homo sapiens
13 CDC73  
Affinity Capture-Western Homo sapiens
14 CPSF6 11052
Affinity Capture-MS Homo sapiens
15 KIF14 9928
Affinity Capture-MS Homo sapiens
16 CSTF2T  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
17 XPO1 7514
Co-fractionation Homo sapiens
18 CEP170P1  
Affinity Capture-MS Homo sapiens
19 SSU72 29101
Affinity Capture-MS Homo sapiens
20 WWOX 51741
Affinity Capture-MS Homo sapiens
21 RIT1 6016
Negative Genetic Homo sapiens
22 UNK  
Affinity Capture-RNA Homo sapiens
23 PAPOLB  
Co-fractionation Homo sapiens
24 DLD 1738
Affinity Capture-MS Homo sapiens
25 SYMPK 8189
Affinity Capture-MS Homo sapiens
26 P4HA1 5033
Affinity Capture-MS Homo sapiens
27 MFAP4 4239
Affinity Capture-MS Homo sapiens
28 CUL3 8452
Affinity Capture-MS Homo sapiens
29 BAG2 9532
Affinity Capture-MS Homo sapiens
30 MECP2 4204
Affinity Capture-MS Homo sapiens
31 CYP1A1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 PPIA 5478
Cross-Linking-MS (XL-MS) Homo sapiens
33 VHL  
Affinity Capture-MS Homo sapiens
34 HSPA8 3312
Affinity Capture-MS Homo sapiens
35 PARK2  
Affinity Capture-MS Homo sapiens
36 HNRNPA1 3178
Affinity Capture-MS Homo sapiens
37 CSTF2 1478
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
38 POLR2A 5430
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
39 WIF1 11197
Affinity Capture-MS Homo sapiens
40 TIMMDC1  
Affinity Capture-MS Homo sapiens
41 C9orf78 51759
Affinity Capture-MS Homo sapiens
42 RPA3 6119
Proximity Label-MS Homo sapiens
43 MUT 4594
Co-fractionation Homo sapiens
44 CCNL2 81669
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 RNPS1 10921
Affinity Capture-MS Homo sapiens
46 TOP1 7150
Affinity Capture-MS Homo sapiens
47 SYT6  
Affinity Capture-MS Homo sapiens
48 HECW2  
Affinity Capture-MS Homo sapiens
49 GTF2E2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 PRPH2 5961
Affinity Capture-MS Homo sapiens
51 NTRK1 4914
Affinity Capture-MS Homo sapiens
52 EGFR 1956
Affinity Capture-MS Homo sapiens
53 PABPC1 26986
Co-fractionation Homo sapiens
54 GAPDH 2597
Co-fractionation Homo sapiens
55 CIT 11113
Affinity Capture-MS Homo sapiens
56 RBM14 10432
Affinity Capture-MS Homo sapiens
57 FIP1L1 81608
Affinity Capture-MS Homo sapiens
58 SERBP1 26135
Affinity Capture-MS Homo sapiens
59 SNW1 22938
Affinity Capture-MS Homo sapiens
60 CUL7 9820
Affinity Capture-MS Homo sapiens
61 SLBP  
Affinity Capture-Western Homo sapiens
62 TGM1 7051
Affinity Capture-MS Homo sapiens
63 BMP1 649
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 PABPC3 5042
Co-fractionation Homo sapiens
65 PAPOLA 10914
Co-fractionation Homo sapiens
66 ITGB4 3691
Co-fractionation Homo sapiens
67 CEBPA  
Protein-peptide Homo sapiens
68 CPSF3 51692
Affinity Capture-MS Homo sapiens
69 C1QL4  
Affinity Capture-MS Homo sapiens
70 FGFR1OP  
Affinity Capture-MS Homo sapiens
71 EP300 2033
Affinity Capture-MS Homo sapiens
72 CDC5L 988
Affinity Capture-MS Homo sapiens
73 CD46 4179
Co-fractionation Homo sapiens
74 HSPA2 3306
Affinity Capture-MS Homo sapiens
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