Gene description for CRTAP
Gene name cartilage associated protein
Gene symbol CRTAP
Other names/aliases CASP
LEPREL3
OI7
P3H5
Species Homo sapiens
 Database cross references - CRTAP
ExoCarta ExoCarta_10491
Entrez Gene 10491
HGNC 2379
MIM 605497
UniProt O75718  
 CRTAP identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Mesenchymal stem cells Unpublished / Not applicable
 Gene ontology annotations for CRTAP
Molecular Function
    protein complex binding GO:0032403 ISS
Biological Process
    peptidyl-proline hydroxylation to 3-hydroxy-L-proline GO:0018400 IEA
    protein stabilization GO:0050821 IMP
    extracellular matrix organization GO:0030198 TAS
    negative regulation of post-translational protein modification GO:1901874 IMP
    chaperone-mediated protein folding GO:0061077 ISS
    spermatogenesis GO:0007283 IEA
Subcellular Localization
    macromolecular complex GO:0032991 ISS
    extracellular space GO:0005615 IDA
    endoplasmic reticulum GO:0005783 IDA
    proteinaceous extracellular matrix GO:0005578 IEA
    endoplasmic reticulum lumen GO:0005788 TAS
 Experiment description of studies that identified CRTAP in exosomes
1
Experiment ID 235
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 126
ISEV standards
✘ EV Biophysical techniques
✔
GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
 Protein-protein interactions for CRTAP
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which CRTAP is involved
PathwayEvidenceSource
Collagen biosynthesis and modifying enzymes TAS Reactome





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