Gene description for HAT1
Gene name histone acetyltransferase 1
Gene symbol HAT1
Other names/aliases KAT1
Species Homo sapiens
 Database cross references - HAT1
ExoCarta ExoCarta_8520
Vesiclepedia VP_8520
Entrez Gene 8520
HGNC 4821
MIM 603053
UniProt O14929  
 HAT1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Chondrocytes 35931686    
Chondrocytes 35931686    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 36706192    
Embryonic kidney cells 36706192    
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Squamous carcinoma cells 20124223    
Thymus 23844026    
 Gene ontology annotations for HAT1
Molecular Function
    histone acetyltransferase activity GO:0004402 TAS
    protein binding GO:0005515 IPI
    histone H4 acetyltransferase activity GO:0010485 IBA
    histone H3K56 acetyltransferase activity GO:0032931 IEA
    histone H3K14 acetyltransferase activity GO:0036408 IEA
    histone binding GO:0042393 IEA
    histone H3K9 acetyltransferase activity GO:0043992 IEA
    histone H3K18 acetyltransferase activity GO:0043993 IEA
    histone H3K23 acetyltransferase activity GO:0043994 IEA
    histone H4K5 acetyltransferase activity GO:0043995 IEA
    histone H4K8 acetyltransferase activity GO:0043996 IEA
    histone H4K12 acetyltransferase activity GO:0043997 IDA
    histone H2AK5 acetyltransferase activity GO:0043999 IEA
    histone H2AK9 acetyltransferase activity GO:0044012 IEA
    histone H2BK5 acetyltransferase activity GO:0044014 IEA
    histone H2BK12 acetyltransferase activity GO:0044015 IEA
    histone H3K4 acetyltransferase activity GO:0044016 IEA
    histone H3K27 acetyltransferase activity GO:0044017 IEA
    histone H3K36 acetyltransferase activity GO:0044018 IEA
    histone H4K16 acetyltransferase activity GO:0046972 IEA
    histone H3K122 acetyltransferase activity GO:0140908 IEA
Biological Process
    nucleosome assembly GO:0006334 IDA
    internal protein amino acid acetylation GO:0006475 TAS
    subtelomeric heterochromatin formation GO:0031509 IEA
    chromosome organization GO:0051276 TAS
Subcellular Localization
    chromosome, telomeric region GO:0000781 HDA
    chromatin GO:0000785 IDA
    nucleus GO:0005634 TAS
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    mitochondrion GO:0005739 IEA
    nuclear matrix GO:0016363 IEA
    protein-containing complex GO:0032991 IDA
 Experiment description of studies that identified HAT1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 426
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 427
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
12
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
13
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
14
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
15
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
16
Experiment ID 1275
MISEV standards
Biophysical techniques
✔
CD9|TSG101|FLOT1|FLOT2|RAB35|RAB5A|GAPDH|TFRC|CD63|Alix
Enriched markers
✔
PCNA|CANX|GAPDH|HSP90AA1|HSP90B1
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36706192    
Organism Homo sapiens
Experiment description Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles
Authors "Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q."
Journal name Sci Adv
Publication year 2023
Sample Embryonic kidney cells
Sample name HEK293T
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
17
Experiment ID 1280
MISEV standards
Biophysical techniques
✔
TSG101|CD9|FLOT1|FLOT2|RAB35|RAB5A|GAPDH|TFRC|CD63|Alix
Enriched markers
✔
PCNA|CANX|GAPDH|HSP90AA1|HSP90B1
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36706192    
Organism Homo sapiens
Experiment description Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles
Authors "Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q."
Journal name Sci Adv
Publication year 2023
Sample Embryonic kidney cells
Sample name HEK293T - Fraction 5
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density 1.151 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
18
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
19
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
20
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
21
Experiment ID 434
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
22
Experiment ID 435
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
23
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔
Alix|CD81|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
24
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for HAT1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PIP4K2C 79837
Co-fractionation Homo sapiens
2 HIST1H4K 8362
Affinity Capture-MS Homo sapiens
3 GATAD2B 57459
Affinity Capture-MS Homo sapiens
4 SPRTN  
Affinity Capture-MS Homo sapiens
5 EEF1G 1937
Cross-Linking-MS (XL-MS) Homo sapiens
6 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-crystal Structure Homo sapiens
Biochemical Activity Homo sapiens
Co-fractionation Homo sapiens
7 TLK2  
Affinity Capture-MS Homo sapiens
8 REL 5966
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
9 CABIN1  
Affinity Capture-MS Homo sapiens
10 C9orf78 51759
Affinity Capture-MS Homo sapiens
11 CSTA 1475
Affinity Capture-MS Homo sapiens
12 KIF5B 3799
Co-fractionation Homo sapiens
13 SAP30 8819
Affinity Capture-MS Homo sapiens
14 RPS6KB2  
Affinity Capture-MS Homo sapiens
15 COX8A  
Proximity Label-MS Homo sapiens
16 VPS29 51699
Co-fractionation Homo sapiens
17 CENPA  
Proximity Label-MS Homo sapiens
18 LRPPRC 10128
Co-fractionation Homo sapiens
19 KIAA1429 25962
Affinity Capture-MS Homo sapiens
20 RNH1 6050
Co-fractionation Homo sapiens
21 CHAF1A  
Co-fractionation Homo sapiens
22 COPB2 9276
Cross-Linking-MS (XL-MS) Homo sapiens
23 CDK2AP1  
Affinity Capture-MS Homo sapiens
24 COPS5 10987
Affinity Capture-MS Homo sapiens
25 ACPP 55
Affinity Capture-MS Homo sapiens
26 ARID4B  
Affinity Capture-MS Homo sapiens
27 ZGPAT  
Affinity Capture-Western Homo sapiens
28 HIST4H4 121504
Biochemical Activity Homo sapiens
29 ZNF512B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 MORN5  
Two-hybrid Homo sapiens
31 TULP3 7289
Affinity Capture-MS Homo sapiens
32 ATRX 546
Affinity Capture-MS Homo sapiens
33 CLTC 1213
Co-fractionation Homo sapiens
34 PHF1  
Affinity Capture-MS Homo sapiens
35 SMARCA1 6594
Affinity Capture-MS Homo sapiens
36 ASF1A 25842
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
37 HIST1H3H 8357
Affinity Capture-MS Homo sapiens
38 MTA2 9219
Affinity Capture-MS Homo sapiens
39 HSPH1 10808
Co-fractionation Homo sapiens
40 YWHAB 7529
Co-fractionation Homo sapiens
41 VTN 7448
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 E2F4  
Affinity Capture-MS Homo sapiens
43 RBBP7 5931
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 UBN2  
Affinity Capture-MS Homo sapiens
45 SHPRH  
Affinity Capture-MS Homo sapiens
46 TTC4  
Co-fractionation Homo sapiens
47 HIST1H3E 8353
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-purification Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 TGM2 7052
Co-fractionation Homo sapiens
49 PARK2  
Affinity Capture-MS Homo sapiens
50 KLC1 3831
Co-fractionation Homo sapiens
51 CCAR2 57805
Co-fractionation Homo sapiens
52 FAM204A  
Affinity Capture-MS Homo sapiens
53 C17orf49 124944
Affinity Capture-MS Homo sapiens
54 RPA4  
Proximity Label-MS Homo sapiens
55 CBX3 11335
Proximity Label-MS Homo sapiens
56 MSMB  
Affinity Capture-MS Homo sapiens
57 HIST1H3J 8356
Affinity Capture-MS Homo sapiens
58 TUBA1B 10376
Affinity Capture-MS Homo sapiens
59 HIST1H4L 8368
Affinity Capture-MS Homo sapiens
60 NTRK1 4914
Affinity Capture-MS Homo sapiens
61 AIM2  
Affinity Capture-MS Homo sapiens
62 IFI16 3428
Affinity Capture-MS Homo sapiens
63 C5orf51 285636
Co-fractionation Homo sapiens
64 BPTF 2186
Affinity Capture-MS Homo sapiens
65 FBXL6  
Affinity Capture-MS Homo sapiens
66 SIN3A  
Affinity Capture-MS Homo sapiens
67 MYBL2 4605
Affinity Capture-MS Homo sapiens
68 HIST2H2AC 8338
Biochemical Activity Homo sapiens
69 YWHAQ 10971
Reconstituted Complex Homo sapiens
70 MBD2 8932
Affinity Capture-MS Homo sapiens
71 CRY2  
Affinity Capture-MS Homo sapiens
72 ACAA1 30
Biochemical Activity Homo sapiens
73 SUDS3  
Affinity Capture-MS Homo sapiens
74 MBD3 53615
Affinity Capture-MS Homo sapiens
75 CEBPA  
Protein-peptide Homo sapiens
76 DAXX  
Affinity Capture-MS Homo sapiens
77 ST13 6767
Co-fractionation Homo sapiens
78 HIST1H4C 8364
Affinity Capture-MS Homo sapiens
79 SEMG1 6406
Affinity Capture-MS Homo sapiens
80 BCL11A 53335
Affinity Capture-MS Homo sapiens
81 GATAD2A 54815
Affinity Capture-MS Homo sapiens
82 STK3 6788
Co-fractionation Homo sapiens
83 HSPA5 3309
Co-fractionation Homo sapiens
84 HIST1H3F 8968
Affinity Capture-MS Homo sapiens
85 ZSCAN4  
Two-hybrid Homo sapiens
86 HIST1H3A 8350
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
87 NASP 4678
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
88 TP53BP1 7158
Affinity Capture-MS Homo sapiens
89 C15orf41  
Affinity Capture-MS Homo sapiens
90 EIF4G1 1981
Co-fractionation Homo sapiens
91 CUL3 8452
Affinity Capture-MS Homo sapiens
92 SLK 9748
Co-fractionation Homo sapiens
93 SEMG2 6407
Affinity Capture-MS Homo sapiens
94 BAP1 8314
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
95 SNF8 11267
Co-fractionation Homo sapiens
96 RBBP4 5928
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
97 HIST3H3 8290
Co-purification Homo sapiens
Affinity Capture-MS Homo sapiens
Protein-peptide Homo sapiens
98 AP2M1 1173
Co-fractionation Homo sapiens
99 HIST1H3B 8358
Affinity Capture-MS Homo sapiens
100 HIST1H4B 8366
Affinity Capture-MS Homo sapiens
101 NRBP1 29959
Co-fractionation Homo sapiens
102 MEOX2  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
103 LIN9  
Affinity Capture-MS Homo sapiens
104 RPA3 6119
Proximity Label-MS Homo sapiens
105 KLK3  
Affinity Capture-MS Homo sapiens
106 CHD3 1107
Affinity Capture-MS Homo sapiens
107 HSPA1A 3303
Co-fractionation Homo sapiens
108 HTR2B  
Two-hybrid Homo sapiens
109 HIST1H4F 8361
Affinity Capture-MS Homo sapiens
110 NMD3 51068
Affinity Capture-MS Homo sapiens
111 KANK4  
Two-hybrid Homo sapiens
112 DDRGK1 65992
Affinity Capture-MS Homo sapiens
113 SEH1L 81929
Affinity Capture-MS Homo sapiens
114 HIST1H4I 8294
Affinity Capture-MS Homo sapiens
115 AZGP1 563
Affinity Capture-MS Homo sapiens
116 PPFIA1 8500
Co-fractionation Homo sapiens
117 ENTHD2  
Two-hybrid Homo sapiens
118 PYHIN1  
Affinity Capture-MS Homo sapiens
119 BRMS1L  
Affinity Capture-MS Homo sapiens
120 H2AFX 3014
Affinity Capture-Western Homo sapiens
121 HIST2H4A 8370
Biochemical Activity Homo sapiens
122 ATG10  
Affinity Capture-MS Homo sapiens
123 CDAN1 146059
Affinity Capture-MS Homo sapiens
124 TLN2 83660
Co-fractionation Homo sapiens
125 BCL2A1  
Co-crystal Structure Homo sapiens
126 UBE3A 7337
Co-fractionation Homo sapiens
127 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
128 PARP1 142
Affinity Capture-MS Homo sapiens
129 BKRF1  
Affinity Capture-MS
130 H3F3A 3020
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
131 SAP130  
Affinity Capture-MS Homo sapiens
132 MTA1 9112
Affinity Capture-MS Homo sapiens
133 TNFAIP1 7126
Two-hybrid Homo sapiens
134 DPY30 84661
Affinity Capture-MS Homo sapiens
135 G3BP1 10146
Affinity Capture-MS Homo sapiens
136 ASF1B  
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
137 CHD4 1108
Affinity Capture-MS Homo sapiens
138 AAGAB  
Affinity Capture-MS Homo sapiens
139 UNK  
Affinity Capture-RNA Homo sapiens
140 ESR1  
Affinity Capture-MS Homo sapiens
141 HMGXB4 10042
Affinity Capture-MS Homo sapiens
142 LARP7 51574
Co-fractionation Homo sapiens
143 TCF4  
Two-hybrid Homo sapiens
144 NCL 4691
Co-fractionation Homo sapiens
145 EZH2  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
146 IPO4 79711
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
147 TRIB3  
Two-hybrid Homo sapiens
148 MTA3 57504
Affinity Capture-MS Homo sapiens
149 HIRA  
Affinity Capture-MS Homo sapiens
150 DDI2 84301
Co-fractionation Homo sapiens
151 C9orf72  
Affinity Capture-MS Homo sapiens
152 GATAD1  
Affinity Capture-MS Homo sapiens
153 UBN1  
Affinity Capture-MS Homo sapiens
154 CHAF1B  
Co-fractionation Homo sapiens
155 PWWP2A  
Affinity Capture-MS Homo sapiens
156 CPSF6 11052
Co-fractionation Homo sapiens
157 KIF4A 24137
Co-fractionation Homo sapiens
158 LIN37  
Affinity Capture-MS Homo sapiens
159 LUC7L3 51747
Co-fractionation Homo sapiens
160 EP300 2033
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which HAT1 is involved
PathwayEvidenceSource
Chromatin modifying enzymes TAS Reactome
Chromatin organization TAS Reactome
HATs acetylate histones TAS Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here