Gene description for POLR2C
Gene name polymerase (RNA) II (DNA directed) polypeptide C, 33kDa
Gene symbol POLR2C
Other names/aliases RPB3
RPB31
hRPB33
hsRPB3
Species Homo sapiens
 Database cross references - POLR2C
ExoCarta ExoCarta_5432
Entrez Gene 5432
HGNC 9189
MIM 180663
UniProt P19387  
 POLR2C identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Thymus 23844026    
 Gene ontology annotations for POLR2C
Molecular Function
    RNA polymerase II activity GO:0001055 IBA
    protein dimerization activity GO:0046983 IEA
    DNA binding GO:0003677 IEA
Biological Process
    mRNA splicing, via spliceosome GO:0000398 TAS
    somatic stem cell maintenance GO:0035019 TAS
    piRNA metabolic process GO:0034587 TAS
    transcription elongation from RNA polymerase II promoter GO:0006368 TAS
    DNA repair GO:0006281 TAS
    transcription-coupled nucleotide-excision repair GO:0006283 TAS
    gene expression GO:0010467 TAS
    viral process GO:0016032 TAS
    transcription initiation from RNA polymerase II promoter GO:0006367 TAS
    nucleotide-excision repair GO:0006289 TAS
    positive regulation of viral transcription GO:0050434 TAS
    transcription from RNA polymerase II promoter GO:0006366 TAS
    7-methylguanosine mRNA capping GO:0006370 TAS
    RNA splicing GO:0008380 TAS
Subcellular Localization
    DNA-directed RNA polymerase II, core complex GO:0005665 IDA
    cytoplasm GO:0005737 IDA
    nucleus GO:0005634 IDA
    microtubule cytoskeleton GO:0015630 IDA
    nucleoplasm GO:0005654 TAS
 Experiment description of studies that identified POLR2C in exosomes
1
Experiment ID 207
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
ISEV standards
✘ EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for POLR2C
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 POLR2B 5431
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
2 POLR2C 5432
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
3 MED19  
Affinity Capture-MS Homo sapiens
4 POLR2H 5437
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
5 POLR2F  
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
6 TAF15 8148
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
7 IXL  
Affinity Capture-MS Homo sapiens
8 EEF1A1 1915
Two-hybrid Homo sapiens
9 POLR2J  
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
10 C14orf1 11161
Two-hybrid Homo sapiens
11 POLR2G  
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
12 POLR2K  
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
13 POLR2L 5441
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
14 MYOG  
Invivo Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
15 POLR2E 5434
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
16 MED9  
Affinity Capture-MS Homo sapiens
17 TRG20  
Affinity Capture-MS Homo sapiens
18 RIF1  
Two-hybrid Homo sapiens
19 POLR2A 5430
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
20 ATF4  
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
21 FLJ11011  
Two-hybrid Homo sapiens
22 CRSP7  
Affinity Capture-MS Homo sapiens
23 STC2 8614
Two-hybrid Homo sapiens
24 MED28  
Affinity Capture-MS Homo sapiens
25 SMARCC2 6601
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which POLR2C is involved
PathwayEvidenceSource
Abortive elongation of HIV-1 transcript in the absence of Tat TAS Reactome
Dual incision reaction in TC-NER TAS Reactome
Formation of HIV elongation complex in the absence of HIV Tat IEA Reactome
Formation of HIV-1 elongation complex containing HIV-1 Tat IEA Reactome
Formation of RNA Pol II elongation complex TAS Reactome
Formation of the Early Elongation Complex TAS Reactome
Formation of the HIV-1 Early Elongation Complex IEA Reactome
Formation of transcription-coupled NER (TC-NER) repair complex TAS Reactome
HIV elongation arrest and recovery IEA Reactome
HIV Transcription Initiation IEA Reactome
MicroRNA (miRNA) biogenesis TAS Reactome
mRNA Capping TAS Reactome
mRNA Splicing - Major Pathway TAS Reactome
mRNA Splicing - Minor Pathway TAS Reactome
Pausing and recovery of HIV elongation TAS Reactome
Pausing and recovery of Tat-mediated HIV elongation TAS Reactome
PIWI-interacting RNA (piRNA) biogenesis TAS Reactome
POU5F1 (OCT4), SOX2, NANOG activate genes related to proliferation TAS Reactome
Processing of Capped Intron-Containing Pre-mRNA TAS Reactome
RNA Pol II CTD phosphorylation and interaction with CE TAS Reactome
RNA Pol II CTD phosphorylation and interaction with CE IEA Reactome
RNA Polymerase II HIV Promoter Escape IEA Reactome
RNA Polymerase II Pre-transcription Events TAS Reactome
RNA Polymerase II Promoter Escape TAS Reactome
RNA Polymerase II Transcription Elongation TAS Reactome
RNA Polymerase II Transcription Initiation TAS Reactome
RNA Polymerase II Transcription Initiation And Promoter Clearance TAS Reactome
RNA Polymerase II Transcription Pre-Initiation And Promoter Opening TAS Reactome
Tat-mediated elongation of the HIV-1 transcript TAS Reactome
Tat-mediated HIV elongation arrest and recovery IEA Reactome
Transcription of the HIV genome IEA Reactome
Transcription-coupled NER (TC-NER) TAS Reactome
Transcriptional regulation by small RNAs TAS Reactome
Transcriptional regulation of pluripotent stem cells TAS Reactome
Viral Messenger RNA Synthesis TAS Reactome





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