Gene description for RPS6KA1
Gene name ribosomal protein S6 kinase, 90kDa, polypeptide 1
Gene symbol RPS6KA1
Other names/aliases HU-1
MAPKAPK1A
RSK
RSK1
p90Rsk
Species Homo sapiens
 Database cross references - RPS6KA1
ExoCarta ExoCarta_6195
Entrez Gene 6195
HGNC 10430
MIM 601684
UniProt Q15418  
 RPS6KA1 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for RPS6KA1
Molecular Function
    protein serine/threonine/tyrosine kinase activity GO:0004712 IDA
    ATP binding GO:0005524 IEA
    protein binding GO:0005515 IPI
    cysteine-type endopeptidase inhibitor activity involved in apoptotic process GO:0043027 IDA
    magnesium ion binding GO:0000287 IEA
    ribosomal protein S6 kinase activity GO:0004711 IBA
    protein serine/threonine kinase activity GO:0004674 IDA
Biological Process
    innate immune response GO:0045087 TAS
    toll-like receptor 2 signaling pathway GO:0034134 TAS
    toll-like receptor TLR1:TLR2 signaling pathway GO:0038123 TAS
    synaptic transmission GO:0007268 TAS
    cell cycle GO:0007049 IEA
    toll-like receptor 10 signaling pathway GO:0034166 TAS
    toll-like receptor 3 signaling pathway GO:0034138 TAS
    neurotrophin TRK receptor signaling pathway GO:0048011 TAS
    signal transduction GO:0007165 TAS
    positive regulation of cell differentiation GO:0045597 TAS
    positive regulation of cell growth GO:0030307 TAS
    negative regulation of apoptotic process GO:0043066 IMP
    intracellular signal transduction GO:0035556 IBA
    regulation of translation in response to stress GO:0043555 TAS
    MyD88-dependent toll-like receptor signaling pathway GO:0002755 TAS
    toll-like receptor 9 signaling pathway GO:0034162 TAS
    toll-like receptor 5 signaling pathway GO:0034146 TAS
    regulation of DNA-templated transcription in response to stress GO:0043620 TAS
    negative regulation of cysteine-type endopeptidase activity involved in apoptotic process GO:0043154 IDA
    positive regulation of hepatic stellate cell activation GO:2000491 IMP
    toll-like receptor signaling pathway GO:0002224 TAS
    protein phosphorylation GO:0006468 IBA
    hepatocyte proliferation GO:0072574 ISS
    stress-activated MAPK cascade GO:0051403 TAS
    MyD88-independent toll-like receptor signaling pathway GO:0002756 TAS
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 IMP
    toll-like receptor TLR6:TLR2 signaling pathway GO:0038124 TAS
    axon guidance GO:0007411 TAS
    toll-like receptor 4 signaling pathway GO:0034142 TAS
    TRIF-dependent toll-like receptor signaling pathway GO:0035666 TAS
Subcellular Localization
    cytosol GO:0005829 TAS
    spindle GO:0005819 IEA
    cytoplasm GO:0005737 IDA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 TAS
 Experiment description of studies that identified RPS6KA1 in exosomes
1
Experiment ID 207
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
ISEV standards
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for RPS6KA1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MAPK1 5594
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
2 NFKBIA  
Invitro Homo sapiens
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
3 TSC1  
Affinity Capture-Western Homo sapiens
4 CARHSP1 23589
Reconstituted Complex Homo sapiens
5 MAPK3 5595
Affinity Capture-Western Homo sapiens
6 TOB2  
Invitro Homo sapiens
Invivo Homo sapiens
7 EEF2K  
Reconstituted Complex Homo sapiens
8 BAD  
Reconstituted Complex Homo sapiens
9 TSC2 7249
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
10 YWHAB 7529
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
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