Gene description for WDR12
Gene name WD repeat domain 12
Gene symbol WDR12
Other names/aliases YTM1
Species Homo sapiens
 Database cross references - WDR12
ExoCarta ExoCarta_55759
Vesiclepedia VP_55759
Entrez Gene 55759
HGNC 14098
UniProt Q9GZL7  
 WDR12 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 34887515    
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for WDR12
Molecular Function
    protein binding GO:0005515 IPI
    ribonucleoprotein complex binding GO:0043021 IEA
Biological Process
    maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) GO:0000463 IBA
    maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) GO:0000463 IMP
    maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) GO:0000466 IBA
    maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) GO:0000466 IMP
    Notch signaling pathway GO:0007219 IEA
    ribosomal large subunit biogenesis GO:0042273 IC
    regulation of cell cycle GO:0051726 IMP
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    nucleolus GO:0005730 IDA
    preribosome, large subunit precursor GO:0030687 IBA
    preribosome, large subunit precursor GO:0030687 IDA
    PeBoW complex GO:0070545 IBA
    PeBoW complex GO:0070545 IDA
 Experiment description of studies that identified WDR12 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
6
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for WDR12
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RIT1 6016
Negative Genetic Homo sapiens
2 SRPK2 6733
Affinity Capture-MS Homo sapiens
3 HIST1H1D 3007
Affinity Capture-MS Homo sapiens
4 RPA2 6118
Proximity Label-MS Homo sapiens
5 TSG101 7251
Co-fractionation Homo sapiens
6 CHCHD1  
Proximity Label-MS Homo sapiens
7 CSNK2A1 1457
Affinity Capture-MS Homo sapiens
8 PNMAL1  
Affinity Capture-MS Homo sapiens
9 VPS29 51699
Co-fractionation Homo sapiens
10 DNAJB6 10049
Proximity Label-MS Homo sapiens
11 USP36  
Affinity Capture-MS Homo sapiens
12 RPL4 6124
Co-fractionation Homo sapiens
13 IGHMBP2 3508
Affinity Capture-MS Homo sapiens
14 KIF23 9493
Affinity Capture-MS Homo sapiens
15 DDX24 57062
Co-fractionation Homo sapiens
16 PPP3CB  
Co-fractionation Homo sapiens
17 PRC1 9055
Affinity Capture-MS Homo sapiens
18 INPP1 3628
Co-fractionation Homo sapiens
19 NPM1 4869
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 WDR12 55759
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
21 OBSL1 23363
Affinity Capture-MS Homo sapiens
22 DDX19A 55308
Co-fractionation Homo sapiens
23 TOM1 10043
Co-fractionation Homo sapiens
24 PRKRA 8575
Affinity Capture-MS Homo sapiens
25 BRD4 23476
Affinity Capture-MS Homo sapiens
26 VAT1 10493
Co-fractionation Homo sapiens
27 WDR5 11091
Affinity Capture-Western Homo sapiens
28 CSNK2B 1460
Co-fractionation Homo sapiens
29 KIF14 9928
Affinity Capture-MS Homo sapiens
30 PES1 23481
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
31 BRIX1 55299
Co-fractionation Homo sapiens
32 CHMP4C 92421
Affinity Capture-MS Homo sapiens
33 XPO1 7514
Co-fractionation Homo sapiens
34 SEPHS1 22929
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
35 CHMP7 91782
Co-fractionation Homo sapiens
36 USP14 9097
Co-fractionation Homo sapiens
37 PARK2  
Affinity Capture-MS Homo sapiens
38 TUFM 7284
Co-fractionation Homo sapiens
39 IFI16 3428
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 HIST1H1E 3008
Affinity Capture-MS Homo sapiens
41 RPL36 25873
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
42 HIST1H1C 3006
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
43 DDX54 79039
Co-fractionation Homo sapiens
44 LYAR 55646
Affinity Capture-MS Homo sapiens
45 HECTD1 25831
Affinity Capture-MS Homo sapiens
46 PPM1G 5496
Affinity Capture-MS Homo sapiens
47 GTPBP4 23560
Co-fractionation Homo sapiens
48 VPS37B 79720
Co-fractionation Homo sapiens
49 ACBD3 64746
Co-fractionation Homo sapiens
50 TXN2 25828
Co-fractionation Homo sapiens
51 ANLN 54443
Affinity Capture-MS Homo sapiens
52 HNRNPDL 9987
Co-fractionation Homo sapiens
53 DDX6 1656
Co-fractionation Homo sapiens
54 TOMM34 10953
Co-fractionation Homo sapiens
55 NOC3L 64318
Co-fractionation Homo sapiens
56 MRTO4 51154
Co-fractionation Homo sapiens
57 RPL13 6137
Affinity Capture-MS Homo sapiens
58 BYSL 705
Co-fractionation Homo sapiens
59 SIRT7  
Affinity Capture-MS Homo sapiens
60 KMT2E  
Affinity Capture-MS Homo sapiens
61 UBE2Z 65264
Co-fractionation Homo sapiens
62 NPM3 10360
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 SRSF5 6430
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
64 RPL3 6122
Affinity Capture-MS Homo sapiens
65 EGFR 1956
Negative Genetic Homo sapiens
66 RPL7L1 285855
Affinity Capture-MS Homo sapiens
67 RPL7 6129
Co-fractionation Homo sapiens
68 TBCD 6904
Co-fractionation Homo sapiens
69 EBNA1BP2 10969
Co-fractionation Homo sapiens
70 PSPC1 55269
Co-fractionation Homo sapiens
71 RPA3 6119
Proximity Label-MS Homo sapiens
72 DNAJC9 23234
Proximity Label-MS Homo sapiens
73 CSNK2A2 1459
Affinity Capture-MS Homo sapiens
74 TSN 7247
Co-fractionation Homo sapiens
75 DDRGK1 65992
Affinity Capture-MS Homo sapiens
76 RNF2  
Affinity Capture-MS Homo sapiens
77 BMI1  
Affinity Capture-MS Homo sapiens
78 SNF8 11267
Co-fractionation Homo sapiens
79 PUS1 80324
Co-fractionation Homo sapiens
80 FTSJ3 117246
Co-fractionation Homo sapiens
81 PPP2CB 5516
Co-fractionation Homo sapiens
82 VPS25 84313
Co-fractionation Homo sapiens
83 VPS36 51028
Co-fractionation Homo sapiens
84 SUGT1 10910
Co-fractionation Homo sapiens
85 GRK5 2869
Affinity Capture-MS Homo sapiens
86 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
87 TBC1D13 54662
Co-fractionation Homo sapiens
88 TP53 7157
Affinity Capture-MS Homo sapiens
89 MNDA 4332
Affinity Capture-MS Homo sapiens
90 WDR92 116143
Co-fractionation Homo sapiens
91 BOP1 23246
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
92 DGCR8  
Affinity Capture-MS Homo sapiens
93 RPUSD2  
Co-fractionation Homo sapiens
94 NIFK 84365
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
95 DDX27 55661
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
96 KRR1 11103
Affinity Capture-MS Homo sapiens
97 NEIL1  
Affinity Capture-MS Homo sapiens
98 WDR55  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
99 IL17B 27190
Affinity Capture-MS Homo sapiens
100 RTCB 51493
Co-fractionation Homo sapiens
101 CIT 11113
Affinity Capture-MS Homo sapiens
102 RPL28 6158
Affinity Capture-MS Homo sapiens
103 KRAS 3845
Negative Genetic Homo sapiens
104 CASQ2 845
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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