ExoCarta: Gene summary

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Gene description for IKBIP
Gene name	IKBKB interacting protein
Gene symbol	IKBIP
Other names/aliases	IKIP
Species	Homo sapiens
Database cross references - IKBIP
ExoCarta	ExoCarta_121457
Vesiclepedia	VP_121457
Entrez Gene	121457
HGNC	26430
MIM	609861
UniProt	Q70UQ0
IKBIP identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells	36408942
Bone marrow mesenchymal stem cells	36408942
Bone marrow mesenchymal stem cells	36408942
Bone marrow mesenchymal stem cells	36408942
Mesenchymal stem cells	36408942

Gene ontology annotations for IKBIP

Molecular Function
	protein binding	GO:0005515	IPI
Biological Process
	response to X-ray	GO:0010165	TAS
Subcellular Localization
	nucleolus	GO:0005730	IDA
	endoplasmic reticulum	GO:0005783	IDA
	endoplasmic reticulum membrane	GO:0005789	TAS
	membrane	GO:0016020	HDA

Experiment description of studies that identified IKBIP in exosomes

Experiment ID

489

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 6

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

490

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 7

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

491

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 8

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

492

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 9

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

488

MISEV standards
✔ EM	Biophysical techniques
✔ CD9\|CD81\|CD63\|GAPDH\|SDCBP\|LAMP1\|TFRC\|UCHL1\|FLOT2\|LAMP2\|FLOT1\|ICAM1\|RAB5B\|CD151\|RAB35\|TSG101\|RAB5A\|CD82	Enriched markers
✔ CANX	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Homo sapiens

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Mesenchymal stem cells

Sample name

UCMSC

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Protein-protein interactions for IKBIP

Protein Interactor

ExoCarta ID

Identification method

PubMed

Species

HNRNPH1

3187

Co-fractionation

Homo sapiens

AP2S1

1175

Affinity Capture-MS

Homo sapiens

GPM6A

2823

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

TNIP1

10318

Affinity Capture-MS

Homo sapiens

VAPA

9218

Affinity Capture-MS

Homo sapiens

ANTXR2

118429

Affinity Capture-MS

Homo sapiens

PAWR

5074

Affinity Capture-MS

Homo sapiens

SCYL1

57410

Affinity Capture-MS

Homo sapiens

TMED4

222068

Co-fractionation

Homo sapiens

RPA2

6118

Proximity Label-MS

Homo sapiens

NUP62

23636

Two-hybrid

Homo sapiens

AGGF1

Affinity Capture-MS

Homo sapiens

RPUSD4

84881

Co-fractionation

Homo sapiens

CLEC12A

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

NDUFB6

4712

Co-fractionation

Homo sapiens

APP

351

Reconstituted Complex

Homo sapiens

RB1CC1

9821

Affinity Capture-MS

Homo sapiens

KIAA1429

25962

Affinity Capture-MS

Homo sapiens

BLOC1S1

Affinity Capture-MS

Homo sapiens

DNAJC10

54431

Proximity Label-MS

Homo sapiens

PCNT

Affinity Capture-MS

Homo sapiens

SCARA3

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

DYRK1A

1859

Affinity Capture-MS

Homo sapiens

EXOC1

55763

Affinity Capture-MS

Homo sapiens

Cep55

74107

Affinity Capture-MS

Mus musculus

FBXO28

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

CCDC53

51019

Affinity Capture-MS		Homo sapiens
Two-hybrid		Homo sapiens

ABI1

10006

Affinity Capture-MS

Homo sapiens

TFIP11

Two-hybrid

Homo sapiens

HIST1H2BA

255626

Affinity Capture-MS

Homo sapiens

SFXN2

Co-fractionation

Homo sapiens

CCDC47

57003

Affinity Capture-MS

Homo sapiens

Ikbip

Affinity Capture-MS

Mus musculus

ATG16L1

55054

Affinity Capture-MS

Homo sapiens

ATP6V0D1

9114

Affinity Capture-MS

Homo sapiens

BTNL2

Affinity Capture-MS

Homo sapiens

LOH12CR1

118426

Affinity Capture-MS

Homo sapiens

KIF3A

11127

Affinity Capture-MS

Homo sapiens

FAM20C

56975

Affinity Capture-MS

Homo sapiens

ATG5

9474

Affinity Capture-MS

Homo sapiens

KIF14

9928

Affinity Capture-MS

Homo sapiens

SERPINB9

5272

Affinity Capture-MS

Homo sapiens

LRRC59

55379

Proximity Label-MS

Homo sapiens

NF2

4771

Affinity Capture-MS

Homo sapiens

GPR25

Two-hybrid

Homo sapiens

HIST1H4A

8359

Affinity Capture-MS

Homo sapiens

FOXI2

Affinity Capture-MS

Homo sapiens

LAMC2

3918

Affinity Capture-MS

Homo sapiens

EXOC4

60412

Affinity Capture-MS

Homo sapiens

LCA5L

Two-hybrid

Homo sapiens

TPM4

7171

Cross-Linking-MS (XL-MS)

Homo sapiens

FIBIN

Affinity Capture-MS

Homo sapiens

RAN

5901

Affinity Capture-MS

Homo sapiens

OGT

8473

Reconstituted Complex

Homo sapiens

DNAJB9

4189

Proximity Label-MS

Homo sapiens

ANGPT4

Affinity Capture-MS

Homo sapiens

ARRDC3

57561

Affinity Capture-MS

Homo sapiens

RPN1

6184

Proximity Label-MS

Homo sapiens

CCDC22

28952

Cross-Linking-MS (XL-MS)

Homo sapiens

DNM2

1785

Affinity Capture-MS

Homo sapiens

L3MBTL2

Affinity Capture-MS

Homo sapiens

DNAJC30

Proximity Label-MS

Homo sapiens

RANBP6

26953

Affinity Capture-MS

Homo sapiens

SEC61B

10952

Proximity Label-MS

Homo sapiens

DNAJB11

51726

Proximity Label-MS

Homo sapiens

ALX3

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

STAM

8027

Affinity Capture-MS

Homo sapiens

P3H1

64175

Affinity Capture-MS

Homo sapiens

PLEKHG6

Affinity Capture-MS

Homo sapiens

OXA1L

Co-fractionation

Homo sapiens

IFITM5

Affinity Capture-MS

Homo sapiens

HSPA5

3309

Proximity Label-MS

Homo sapiens

CANX

821

Affinity Capture-MS

Homo sapiens

ITIH3

3699

Affinity Capture-MS

Homo sapiens

CALR3

Proximity Label-MS

Homo sapiens

COMMD8

54951

Affinity Capture-MS

Homo sapiens

NCKAP1

10787

Affinity Capture-MS

Homo sapiens

MYOG

Two-hybrid		Homo sapiens
Two-hybrid		Homo sapiens

TXLNB

Affinity Capture-MS

Homo sapiens

HYPK

25764

Affinity Capture-MS

Homo sapiens

IFT57

55081

Affinity Capture-MS

Homo sapiens

SRSF5

6430

Co-fractionation

Homo sapiens

MAB21L2

Affinity Capture-MS

Homo sapiens

SYNCRIP

10492

Co-fractionation

Homo sapiens

FBXO6

26270

Affinity Capture-MS

Homo sapiens

FAM63B

54629

Affinity Capture-MS

Homo sapiens

MORF4L1

Affinity Capture-MS

Homo sapiens

CYFIP2

26999

Affinity Capture-MS

Homo sapiens

CDK5RAP2

55755

Affinity Capture-MS

Homo sapiens

KRT15

3866

Two-hybrid

Homo sapiens

TPM1

7168

Affinity Capture-MS

Homo sapiens

MSR1

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

PDGFRL

5157

Affinity Capture-MS

Homo sapiens

RPA3

6119

Proximity Label-MS

Homo sapiens

ATP2A1

487

Proximity Label-MS

Homo sapiens

BAP1

8314

Affinity Capture-MS

Homo sapiens

HSPA1A

3303

Cross-Linking-MS (XL-MS)

Homo sapiens

PSMC5

5705

Affinity Capture-MS

Homo sapiens

PPP1R10

Affinity Capture-MS

Homo sapiens

100

DNAJC3

5611

Proximity Label-MS

Homo sapiens

101

SNX2

6643

Affinity Capture-MS

Homo sapiens

102

CERS6

Affinity Capture-MS

Homo sapiens

103

THEG

Affinity Capture-MS

Homo sapiens

104

BIRC8

Affinity Capture-MS

Homo sapiens

105

C9orf16

Two-hybrid

Homo sapiens

106

HUWE1

10075

Affinity Capture-MS

Homo sapiens

107

TPM3

7170

Two-hybrid		Homo sapiens
Two-hybrid		Homo sapiens

108

TRAF7

Affinity Capture-MS

Homo sapiens

109

LAMB1

3912

Affinity Capture-MS

Homo sapiens

110

COLEC12

81035

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

111

FADS3

3995

Affinity Capture-MS

Homo sapiens

112

BLOC1S2

282991

Affinity Capture-MS

Homo sapiens

113

CLEC11A

6320

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

114

VPS52

6293

Affinity Capture-MS

Homo sapiens

115

PDIA4

9601

Proximity Label-MS

Homo sapiens

116

PCDH9

Affinity Capture-MS

Homo sapiens

117

CALU

813

Proximity Label-MS

Homo sapiens

118

SPINK2

Affinity Capture-MS

Homo sapiens

119

PDCD6

10016

Co-fractionation

Homo sapiens

120

CMTM3

123920

Two-hybrid

Homo sapiens

121

EXTL3

2137

Affinity Capture-MS

Homo sapiens

122

ELAVL1

1994

Affinity Capture-RNA

Homo sapiens

123

MDH2

4191

Cross-Linking-MS (XL-MS)

Homo sapiens

124

SNAPIN

23557

Two-hybrid		Homo sapiens
Affinity Capture-MS		Homo sapiens

125

ZNF383

Affinity Capture-MS

Homo sapiens

126

C10orf32

Affinity Capture-MS

Homo sapiens

127

ABI2

10152

Affinity Capture-MS

Homo sapiens

128

KDM1A

23028

Two-hybrid

Homo sapiens

129

STAG3L4

Affinity Capture-MS

Homo sapiens

130

SEC62

7095

Proximity Label-MS

Homo sapiens

131

FLNA

2316

Co-fractionation

Homo sapiens

132

TTC29

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

133

LAMB3

3914

Affinity Capture-MS

Homo sapiens

134

DUSP22

Affinity Capture-MS

Homo sapiens

135

CYFIP1

23191

Affinity Capture-MS

Homo sapiens

136

HSF2BP

Affinity Capture-MS

Homo sapiens

137

TOP1MT

Cross-Linking-MS (XL-MS)

Homo sapiens

138

ESCO2

Affinity Capture-MS

Homo sapiens

View the network

Pathways in which IKBIP is involved

Pathway	Evidence	Source
Cytokine Signaling in Immune system	TAS	Reactome
Immune System	TAS	Reactome
Innate Immune System	TAS	Reactome
Interleukin-1 family signaling	TAS	Reactome
Interleukin-1 signaling	TAS	Reactome
MyD88 cascade initiated on plasma membrane	TAS	Reactome
MyD88 dependent cascade initiated on endosome	TAS	Reactome
MyD88-independent TLR4 cascade	TAS	Reactome
MyD88:MAL(TIRAP) cascade initiated on plasma membrane	TAS	Reactome
Regulation of NF-kappa B signaling	TAS	Reactome
Signaling by Interleukins	TAS	Reactome
TAK1-dependent IKK and NF-kappa-B activation	TAS	Reactome
Toll Like Receptor 10 (TLR10) Cascade	TAS	Reactome
Toll Like Receptor 2 (TLR2) Cascade	TAS	Reactome
Toll Like Receptor 3 (TLR3) Cascade	TAS	Reactome
Toll Like Receptor 4 (TLR4) Cascade	TAS	Reactome
Toll Like Receptor 5 (TLR5) Cascade	TAS	Reactome
Toll Like Receptor 7/8 (TLR7/8) Cascade	TAS	Reactome
Toll Like Receptor 9 (TLR9) Cascade	TAS	Reactome
Toll Like Receptor TLR1:TLR2 Cascade	TAS	Reactome
Toll Like Receptor TLR6:TLR2 Cascade	TAS	Reactome
Toll-like Receptor Cascades	TAS	Reactome
TRAF6 mediated induction of NFkB and MAP kinases upon TLR7/8 or 9 activation	TAS	Reactome
TRIF (TICAM1)-mediated TLR4 signaling	TAS	Reactome

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