Gene description for SLC4A1
Gene name solute carrier family 4 (anion exchanger), member 1 (Diego blood group)
Gene symbol SLC4A1
Other names/aliases AE1
BND3
CD233
DI
EMPB3
EPB3
FR
RTA1A
SW
WD
WD1
WR
Species Homo sapiens
 Database cross references - SLC4A1
ExoCarta ExoCarta_6521
Vesiclepedia VP_6521
Entrez Gene 6521
HGNC 11027
MIM 109270
UniProt P02730  
 SLC4A1 identified in sEVs derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Urine 19056867    
 Gene ontology annotations for SLC4A1
Molecular Function
    solute:inorganic anion antiporter activity GO:0005452 IDA
    solute:inorganic anion antiporter activity GO:0005452 TAS
    protein binding GO:0005515 IPI
    monoatomic anion transmembrane transporter activity GO:0008509 TAS
    bicarbonate transmembrane transporter activity GO:0015106 IBA
    bicarbonate transmembrane transporter activity GO:0015106 IDA
    chloride transmembrane transporter activity GO:0015108 ISS
    hemoglobin binding GO:0030492 IEA
    ankyrin binding GO:0030506 IPI
    protein homodimerization activity GO:0042803 IPI
    protein-membrane adaptor activity GO:0043495 TAS
    chloride:bicarbonate antiporter activity GO:0140900 IDA
Biological Process
    monoatomic anion transport GO:0006820 TAS
    chloride transport GO:0006821 ISS
    intracellular monoatomic ion homeostasis GO:0006873 TAS
    blood coagulation GO:0007596 IEA
    bicarbonate transport GO:0015701 IBA
    bicarbonate transport GO:0015701 IDA
    bicarbonate transport GO:0015701 TAS
    plasma membrane phospholipid scrambling GO:0017121 IEA
    negative regulation of urine volume GO:0035811 IEA
    pH elevation GO:0045852 IEA
    erythrocyte development GO:0048821 IEA
    regulation of intracellular pH GO:0051453 IBA
    transmembrane transport GO:0055085 IBA
    protein localization to plasma membrane GO:0072659 IEA
    chloride transmembrane transport GO:1902476 IDA
    negative regulation of glycolytic process through fructose-6-phosphate GO:1904539 IEA
Subcellular Localization
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    cytoplasmic side of plasma membrane GO:0009898 IEA
    membrane GO:0016020 NAS
    basolateral plasma membrane GO:0016323 IBA
    basolateral plasma membrane GO:0016323 IDA
    Z disc GO:0030018 ISS
    cortical cytoskeleton GO:0030863 IDA
    extracellular exosome GO:0070062 HDA
    blood microparticle GO:0072562 HDA
    ankyrin-1 complex GO:0170014 IDA
 Experiment description of studies that identified SLC4A1 in sEVs
1
Experiment ID 494
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Osteoarthritic cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 496
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Healthy cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for SLC4A1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 VDAC2 7417
Co-fractionation Homo sapiens
2 TOMM40 10452
Co-fractionation Homo sapiens
3 SYK 6850
Biochemical Activity Homo sapiens
4 SLC4A1 6521
Co-crystal Structure Homo sapiens
Co-crystal Structure Homo sapiens
5 KAT2B  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
6 SLC4A1AP  
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
7 VDAC3 7419
Co-fractionation Homo sapiens
8 ANK1 286
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
9 BRD2  
Affinity Capture-MS Homo sapiens
10 HADHB 3032
Co-fractionation Homo sapiens
11 CA2 760
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
12 BRD3 8019
Affinity Capture-MS Homo sapiens
13 CANX 821
Affinity Capture-Western Homo sapiens
14 HBA1 3039
Affinity Capture-Western Homo sapiens
15 CLU 1191
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
16 CDKN2A 1029
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
17 CA4 762
Co-fractionation Homo sapiens
Reconstituted Complex Homo sapiens
18 EPB42 2038
Protein-peptide Homo sapiens
19 GBAS 2631
Co-fractionation Homo sapiens
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