Gene description for TAF6
Gene name TAF6 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 80kDa
Gene symbol TAF6
Other names/aliases MGC:8964
TAF(II)70
TAF(II)80
TAF2E
TAFII-70
TAFII-80
TAFII70
TAFII80
TAFII85
Species Homo sapiens
 Database cross references - TAF6
ExoCarta ExoCarta_6878
Entrez Gene 6878
HGNC 11540
MIM 602955
UniProt P49848  
 TAF6 identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
 Gene ontology annotations for TAF6
Molecular Function
    protein heterodimerization activity GO:0046982 IEA
    DNA binding GO:0003677 IDA
    sequence-specific DNA binding transcription factor activity GO:0003700 IDA
    protein binding GO:0005515 IPI
Biological Process
    gene expression GO:0010467 TAS
    regulation of transcription, DNA-templated GO:0006355 IDA
    DNA-templated transcription, initiation GO:0006352 IDA
    transcription from RNA polymerase II promoter GO:0006366 TAS
    transcription initiation from RNA polymerase II promoter GO:0006367 TAS
    transcription elongation from RNA polymerase II promoter GO:0006368 TAS
    viral process GO:0016032 TAS
    negative regulation of cell proliferation GO:0008285 IDA
    negative regulation of cell cycle GO:0045786 IDA
    regulation of sequence-specific DNA binding transcription factor activity GO:0051090 IEA
Subcellular Localization
    MLL1 complex GO:0071339 IDA
    transcription factor TFTC complex GO:0033276 IDA
    transcription factor TFIID complex GO:0005669 IDA
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 IDA
    nucleoplasm GO:0005654 TAS
 Experiment description of studies that identified TAF6 in exosomes
1
Experiment ID 237
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for TAF6
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TAF5  
Invivo Homo sapiens
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
2 TBP  
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
3 TAF6 6878
Invivo Homo sapiens
Invitro Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
4 SH3KBP1 30011
Affinity Capture-Western Homo sapiens
5 RELA  
Invitro Homo sapiens
6 GTF2E1  
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
7 TAF1  
Reconstituted Complex Homo sapiens
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