Gene description for DHX38
Gene name DEAH (Asp-Glu-Ala-His) box polypeptide 38
Gene symbol DHX38
Other names/aliases DDX38
PRP16
PRPF16
Species Homo sapiens
 Database cross references - DHX38
ExoCarta ExoCarta_9785
Vesiclepedia VP_9785
Entrez Gene 9785
HGNC 17211
MIM 605584
UniProt Q92620  
 DHX38 identified in exosomes derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
 Gene ontology annotations for DHX38
Molecular Function
    DNA clamp loader activity GO:0003689 IEA
    RNA binding GO:0003723 HDA
    RNA binding GO:0003723 IBA
    RNA helicase activity GO:0003724 TAS
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ATP hydrolysis activity GO:0016887 IEA
    3'-5' RNA helicase activity GO:0034458 IBA
    cohesin loader activity GO:0061775 IEA
    chromatin extrusion motor activity GO:0140584 IEA
    ATP-dependent H3-H4 histone complex chaperone activity GO:0140665 IEA
    ATP-dependent H2AZ histone chaperone activity GO:0140849 IEA
Biological Process
    mRNA splicing, via spliceosome GO:0000398 IBA
    mRNA splicing, via spliceosome GO:0000398 IC
    mRNA splicing, via spliceosome GO:0000398 IDA
    mRNA splicing, via spliceosome GO:0000398 TAS
    chromatin remodeling GO:0006338 IEA
    chromatin looping GO:0140588 IEA
Subcellular Localization
    nucleus GO:0005634 NAS
    nucleoplasm GO:0005654 TAS
    spliceosomal complex GO:0005681 IBA
    membrane GO:0016020 HDA
    catalytic step 2 spliceosome GO:0071013 IDA
 Experiment description of studies that identified DHX38 in exosomes
1
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for DHX38
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GNL2 29889
Affinity Capture-MS Homo sapiens
2 RIT1 6016
Negative Genetic Homo sapiens
3 Smek1  
Affinity Capture-MS Mus musculus
4 UTRN 7402
Co-fractionation Homo sapiens
5 COIL  
Proximity Label-MS Homo sapiens
6 Prpf8 192159
Affinity Capture-MS Mus musculus
7 DUXAP10  
Affinity Capture-MS Homo sapiens
8 ZNHIT2 741
Affinity Capture-MS Homo sapiens
9 HIST1H2BG 8339
Proximity Label-MS Homo sapiens
10 DHX8 1659
Proximity Label-MS Homo sapiens
11 BRD2  
Affinity Capture-MS Homo sapiens
12 CSNK2A1 1457
Biochemical Activity Homo sapiens
13 TERF2IP 54386
Two-hybrid Homo sapiens
14 AAR2 25980
Affinity Capture-MS Homo sapiens
15 COPA 1314
Co-fractionation Homo sapiens
16 PRKDC 5591
Co-fractionation Homo sapiens
17 TGM2 7052
Co-fractionation Homo sapiens
18 DDX23 9416
Proximity Label-MS Homo sapiens
19 PARK2  
Affinity Capture-MS Homo sapiens
20 RPS20 6224
Co-fractionation Homo sapiens
21 RNPS1 10921
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 USP39 10713
Affinity Capture-MS Homo sapiens
23 KHDRBS1 10657
Affinity Capture-MS Homo sapiens
24 EZH1  
Affinity Capture-MS Homo sapiens
25 PPP4R2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 SNRPD3 6634
Co-fractionation Homo sapiens
27 SLX4  
Affinity Capture-MS Homo sapiens
28 U2AF1 7307
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
29 NUDCD1 84955
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 SERF2 10169
Affinity Capture-MS Homo sapiens
31 LDLRAD4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 SNX27 81609
Affinity Capture-MS Homo sapiens
33 BTRC 8945
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 FAM177A1 283635
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 RBM8A 9939
Co-fractionation Homo sapiens
36 DDX58 23586
Affinity Capture-RNA Homo sapiens
37 HSPA8 3312
Co-fractionation Homo sapiens
38 LSM2 57819
Co-fractionation Homo sapiens
39 RBMX 27316
Cross-Linking-MS (XL-MS) Homo sapiens
40 JMJD6 23210
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 TMEM14B  
Affinity Capture-MS Homo sapiens
42 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
43 ANTXR1 84168
Affinity Capture-MS Homo sapiens
44 ZNF691  
Affinity Capture-MS Homo sapiens
45 NUP50 10762
Proximity Label-MS Homo sapiens
46 SMEK1 55671
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 CBX3 11335
Proximity Label-MS Homo sapiens
48 ACACA 31
Positive Genetic Homo sapiens
49 AKR7L 246181
Affinity Capture-MS Homo sapiens
50 C16orf58 64755
Affinity Capture-MS Homo sapiens
51 DDX5 1655
Co-fractionation Homo sapiens
52 NTRK1 4914
Affinity Capture-MS Homo sapiens
53 IFI16 3428
Affinity Capture-MS Homo sapiens
54 RPL36 25873
Cross-Linking-MS (XL-MS) Homo sapiens
55 ANAPC15  
Affinity Capture-MS Homo sapiens
56 DDX39B 7919
Co-fractionation Homo sapiens
57 CYLD  
Affinity Capture-MS Homo sapiens
58 RBM10  
Two-hybrid Homo sapiens
Affinity Capture-Luminescence Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Luminescence Homo sapiens
59 CD3EAP  
Proximity Label-MS Homo sapiens
60 HSPA5 3309
Co-fractionation Homo sapiens
61 PSMA3 5684
Co-fractionation Homo sapiens
62 CDK7 1022
Affinity Capture-MS Homo sapiens
63 ZHX1-C8orf76  
Affinity Capture-MS Homo sapiens
64 SMEK2  
Affinity Capture-MS Homo sapiens
65 PSENEN  
Affinity Capture-MS Homo sapiens
66 SMARCA5 8467
Co-fractionation Homo sapiens
67 SIRT7  
Affinity Capture-MS Homo sapiens
68 PITHD1 57095
Co-fractionation Homo sapiens
69 CD2BP2 10421
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 NEBL 10529
Affinity Capture-MS Homo sapiens
71 CUL3 8452
Affinity Capture-MS Homo sapiens
72 EGFR 1956
Affinity Capture-MS Homo sapiens
73 CDKL5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
74 TSSC4  
Affinity Capture-MS Homo sapiens
75 NXF1 10482
Affinity Capture-RNA Homo sapiens
76 ASB12  
Affinity Capture-MS Homo sapiens
77 LBH  
Affinity Capture-MS Homo sapiens
78 MFAP1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
79 RPA3 6119
Proximity Label-MS Homo sapiens
80 VRK2 7444
Affinity Capture-MS Homo sapiens
81 SKIV2L2 23517
Co-fractionation Homo sapiens
82 CERS6  
Affinity Capture-MS Homo sapiens
83 FAM107B 83641
Affinity Capture-MS Homo sapiens
84 CLTC 1213
Co-fractionation Homo sapiens
85 SLU7  
Co-fractionation Homo sapiens
86 BIRC8  
Affinity Capture-MS Homo sapiens
87 RBBP5 5929
Co-fractionation Homo sapiens
88 LMNA 4000
Proximity Label-MS Homo sapiens
89 BRD3 8019
Affinity Capture-MS Homo sapiens
90 PSMB8 5696
Co-fractionation Homo sapiens
91 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
92 PPP4C 5531
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
93 EAPP  
Affinity Capture-MS Homo sapiens
94 SIRT6  
Affinity Capture-MS Homo sapiens
95 CWC27 10283
Co-fractionation Homo sapiens
96 MEN1 4221
Affinity Capture-MS Homo sapiens
97 AURKC  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
98 SLC7A6OS  
Affinity Capture-MS Homo sapiens
99 SHMT2 6472
Affinity Capture-RNA Homo sapiens
100 GPRC5D  
Affinity Capture-MS Homo sapiens
101 IFIT1B  
Affinity Capture-MS Homo sapiens
102 DHX40  
Proximity Label-MS Homo sapiens
103 SLC2A7  
Affinity Capture-MS Homo sapiens
104 SNRNP40 9410
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
105 GPKOW  
Two-hybrid Homo sapiens
Affinity Capture-Luminescence Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Luminescence Homo sapiens
106 DHX16 8449
Two-hybrid Homo sapiens
Affinity Capture-Luminescence Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Luminescence Homo sapiens
107 MOV10 4343
Affinity Capture-RNA Homo sapiens
108 CIT 11113
Affinity Capture-MS Homo sapiens
109 SNW1 22938
Co-fractionation Homo sapiens
110 KRAS 3845
Negative Genetic Homo sapiens
111 CYCS 54205
Affinity Capture-MS Homo sapiens
112 DBR1  
Co-fractionation Homo sapiens
113 EP300 2033
Affinity Capture-MS Homo sapiens
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