Gene description for PTGES2
Gene name prostaglandin E synthase 2
Gene symbol PTGES2
Other names/aliases C9orf15
GBF-1
GBF1
PGES2
mPGES-2
Species Homo sapiens
 Database cross references - PTGES2
ExoCarta ExoCarta_80142
Vesiclepedia VP_80142
Entrez Gene 80142
HGNC 17822
MIM 608152
UniProt Q9H7Z7  
 PTGES2 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for PTGES2
Molecular Function
    DNA binding GO:0003677 IEA
    protein binding GO:0005515 IPI
    lyase activity GO:0016829 IDA
    lyase activity GO:0016829 ISS
    heme binding GO:0020037 ISS
    12-hydroxyheptadecatrienoic acid synthase activity GO:0036134 IEA
    glutathione binding GO:0043295 ISS
    prostaglandin-E synthase activity GO:0050220 IBA
    prostaglandin-E synthase activity GO:0050220 IDA
    prostaglandin-E synthase activity GO:0050220 TAS
Biological Process
    lipid metabolic process GO:0006629 IDA
    cyclooxygenase pathway GO:0019371 TAS
    positive regulation of DNA-templated transcription GO:0045893 IEA
    secretion GO:0046903 IEA
Subcellular Localization
    Golgi membrane GO:0000139 IDA
    extracellular region GO:0005576 TAS
    nucleus GO:0005634 IEA
    mitochondrion GO:0005739 HDA
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    azurophil granule lumen GO:0035578 TAS
    perinuclear region of cytoplasm GO:0048471 IEA
 Experiment description of studies that identified PTGES2 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PTGES2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MAGEA8  
Affinity Capture-MS Homo sapiens
2 ATP6V0D1 9114
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 ATP6V1B2 526
Co-fractionation Homo sapiens
4 EXOSC10 5394
Two-hybrid Homo sapiens
5 MLC1 23209
Affinity Capture-MS Homo sapiens
6 METTL18  
Affinity Capture-MS Homo sapiens
7 C15orf48  
Affinity Capture-MS Homo sapiens
8 ESRRA  
Affinity Capture-MS Homo sapiens
9 POR 5447
Co-fractionation Homo sapiens
10 STK24 8428
Affinity Capture-MS Homo sapiens
11 SHISA2 387914
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 C5orf28  
Affinity Capture-MS Homo sapiens
13 UQCRQ 27089
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 HADHA 3030
Co-fractionation Homo sapiens
15 FUZ 80199
Affinity Capture-MS Homo sapiens
16 LRRC59 55379
Co-fractionation Homo sapiens
17 USP1 7398
Affinity Capture-MS Homo sapiens
18 HIST1H2BC 8347
Cross-Linking-MS (XL-MS) Homo sapiens
19 HEATR6  
Affinity Capture-MS Homo sapiens
20 E2F4  
Affinity Capture-MS Homo sapiens
21 PTGES2 80142
Co-crystal Structure Homo sapiens
Co-crystal Structure Homo sapiens
22 MGRN1 23295
Affinity Capture-MS Homo sapiens
23 ADRB2  
Two-hybrid Homo sapiens
24 UQCRB 7381
Affinity Capture-MS Homo sapiens
25 ATRN 8455
Affinity Capture-MS Homo sapiens
26 CHMP1A 5119
Affinity Capture-MS Homo sapiens
27 LRP1 4035
Affinity Capture-MS Homo sapiens
28 LMNA 4000
Proximity Label-MS Homo sapiens
29 APOE 348
Co-fractionation Homo sapiens
30 SHISA3  
Affinity Capture-MS Homo sapiens
31 CPNE9  
Affinity Capture-MS Homo sapiens
32 UQCR10 29796
Affinity Capture-MS Homo sapiens
33 BRD3 8019
Cross-Linking-MS (XL-MS) Homo sapiens
34 NDUFA4 4697
Affinity Capture-MS Homo sapiens
35 NUP35 129401
Proximity Label-MS Homo sapiens
36 HIST1H4A 8359
Cross-Linking-MS (XL-MS) Homo sapiens
37 PDHA1 5160
Co-fractionation Homo sapiens
38 BTF3L4 91408
Affinity Capture-MS Homo sapiens
39 NDUFA8 4702
Co-fractionation Homo sapiens
40 SBDS 51119
Affinity Capture-MS Homo sapiens
41 INS 3630
Affinity Capture-MS Homo sapiens
42 PUS7 54517
Affinity Capture-MS Homo sapiens
43 SFXN1 94081
Proximity Label-MS Homo sapiens
44 HCCS 3052
Affinity Capture-MS Homo sapiens
45 HTT 3064
Affinity Capture-Luminescence Homo sapiens
46 KIAA1429 25962
Affinity Capture-MS Homo sapiens
47 NF1 4763
Affinity Capture-MS Homo sapiens
48 PDCD10 11235
Affinity Capture-MS Homo sapiens
49 BIRC6 57448
Affinity Capture-MS Homo sapiens
50 ABCF2 10061
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 CYTB  
Affinity Capture-MS Homo sapiens
52 WDR48 57599
Affinity Capture-MS Homo sapiens
53 DLK1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 MYC  
Affinity Capture-MS Homo sapiens
55 UQCRC2 7385
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
56 HIST1H2BA 255626
Cross-Linking-MS (XL-MS) Homo sapiens
57 EID3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 COQ9  
Affinity Capture-MS Homo sapiens
59 SCGB2A2  
Affinity Capture-MS Homo sapiens
60 COX7A2L 9167
Affinity Capture-MS Homo sapiens
61 CYB5R3 1727
Co-fractionation Homo sapiens
62 HIST1H3A 8350
Cross-Linking-MS (XL-MS) Homo sapiens
63 HIST2H3A 333932
Cross-Linking-MS (XL-MS) Homo sapiens
64 SERBP1 26135
Affinity Capture-MS Homo sapiens
65 CYC1 1537
Affinity Capture-MS Homo sapiens
66 UQCRC1 7384
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
67 SUMF1 285362
Affinity Capture-MS Homo sapiens
68 SLC25A12 8604
Proximity Label-MS Homo sapiens
69 UQCR11  
Co-fractionation Homo sapiens
70 HADHB 3032
Co-fractionation Homo sapiens
71 HIST1H2BK 85236
Cross-Linking-MS (XL-MS) Homo sapiens
72 UQCRFS1 7386
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
73 RPA2 6118
Proximity Label-MS Homo sapiens
74 AGPS 8540
Co-fractionation Homo sapiens
75 RANBP6 26953
Affinity Capture-MS Homo sapiens
76 OPLAH 26873
Affinity Capture-MS Homo sapiens
77 GNG5 2787
Co-fractionation Homo sapiens
78 PBXIP1 57326
Affinity Capture-MS Homo sapiens
79 ATP5B 506
Co-fractionation Homo sapiens
80 C1orf189  
Two-hybrid Homo sapiens
81 SYT6  
Affinity Capture-MS Homo sapiens
82 H2AFX 3014
Affinity Capture-MS Homo sapiens
83 HYPM  
Affinity Capture-MS Homo sapiens
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