Gene description for KPNA3
Gene name karyopherin alpha 3 (importin alpha 4)
Gene symbol KPNA3
Other names/aliases IPOA4
SRP1
SRP1gamma
SRP4
hSRP1
Species Homo sapiens
 Database cross references - KPNA3
ExoCarta ExoCarta_3839
Entrez Gene 3839
HGNC 6396
MIM 601892
UniProt O00505  
 KPNA3 identified in exosomes derived from the following tissue/cell type
Endothelial cells 26027894    
 Gene ontology annotations for KPNA3
Molecular Function
    protein C-terminus binding GO:0008022 IEA
    protein transporter activity GO:0008565 IEA
    protein binding GO:0005515 IPI
    nuclear localization sequence binding GO:0008139 TAS
Biological Process
    viral entry into host cell GO:0046718 IEA
    viral penetration into host nucleus GO:0075732 IEA
    protein complex assembly GO:0006461 TAS
    cytokine-mediated signaling pathway GO:0019221 TAS
    NLS-bearing protein import into nucleus GO:0006607 TAS
Subcellular Localization
    nuclear pore GO:0005643 TAS
    nucleoplasm GO:0005654 TAS
    cytosol GO:0005829 TAS
 Experiment description of studies that identified KPNA3 in exosomes
1
Experiment ID 226
ISEV standards
EM
EV Biophysical techniques
GAPDH
EV Cytosolic markers
CD9|FLOT1
EV Membrane markers
LMNA|H2AFX|ATP5A1|TOMM20
EV Negative markers
EV Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting
Authors Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
 Protein-protein interactions for KPNA3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NFKB1 4790
Affinity Capture-MS Homo sapiens
2 TGM2 7052
Affinity Capture-MS Homo sapiens
3 KPNB1 3837
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
4 HNRNPC 3183
Two-hybrid Homo sapiens
5 ARRB1 408
Affinity Capture-MS Homo sapiens
6 ARRB2  
Affinity Capture-MS Homo sapiens
7 RCC1 1104
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
8 FAM50B  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which KPNA3 is involved
PathwayEvidenceSource
ISG15 antiviral mechanism TAS Reactome





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