Gene description for POLR3A
Gene name polymerase (RNA) III (DNA directed) polypeptide A, 155kDa
Gene symbol POLR3A
Other names/aliases ADDH
HLD7
RPC1
RPC155
hRPC155
Species Homo sapiens
 Database cross references - POLR3A
ExoCarta ExoCarta_11128
Vesiclepedia VP_11128
Entrez Gene 11128
HGNC 30074
MIM 614258
UniProt O14802  
 POLR3A identified in sEVs derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for POLR3A
Molecular Function
    magnesium ion binding GO:0000287 IDA
    RNA polymerase I activity GO:0001054 IEA
    RNA polymerase II activity GO:0001055 IEA
    RNA polymerase III activity GO:0001056 IBA
    RNA polymerase IV activity GO:0001057 IEA
    RNA polymerase V activity GO:0001058 IEA
    mitochondrial single subunit type RNA polymerase activity GO:0001065 IEA
    plastid single subunit type RNA polymerase activity GO:0001066 IEA
    DNA binding GO:0003677 IEA
    chromatin binding GO:0003682 IEA
    DNA-directed 5'-3' RNA polymerase activity GO:0003899 IDA
    DNA-directed 5'-3' RNA polymerase activity GO:0003899 NAS
    protein binding GO:0005515 IPI
    zinc ion binding GO:0008270 IDA
    DNA/RNA hybrid binding GO:0071667 IDA
Biological Process
    transcription by RNA polymerase IV GO:0001059 IEA
    transcription by RNA polymerase V GO:0001060 IEA
    DNA-templated transcription GO:0006351 NAS
    transcription by RNA polymerase I GO:0006360 IEA
    transcription by RNA polymerase II GO:0006366 IEA
    mitochondrial transcription GO:0006390 IEA
    positive regulation of interferon-beta production GO:0032728 IMP
    plastid transcription GO:0042793 IEA
    tRNA transcription by RNA polymerase III GO:0042797 IDA
    innate immune response GO:0045087 IMP
    defense response to virus GO:0051607 IEA
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    RNA polymerase III complex GO:0005666 IBA
    RNA polymerase III complex GO:0005666 IDA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
    membrane GO:0016020 HDA
 Experiment description of studies that identified POLR3A in sEVs
1
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for POLR3A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CD3EAP  
Affinity Capture-MS Homo sapiens
2 POLR2G 5436
Affinity Capture-MS Homo sapiens
3 RIT1 6016
Negative Genetic Homo sapiens
4 TRMT1L 81627
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 CHIC1  
Affinity Capture-MS Homo sapiens
6 HDAC2 3066
Co-fractionation Homo sapiens
7 MRPL17  
Co-fractionation Homo sapiens
8 PKP2 5318
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
9 RPAP2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 CDH5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 ZNHIT2 741
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 MRPL54  
Co-fractionation Homo sapiens
13 MRPL24  
Co-fractionation Homo sapiens
14 CSNK2A1 1457
Affinity Capture-MS Homo sapiens
15 LOC101060521 101060521
Co-fractionation Homo sapiens
16 URI1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 PPP1CC 5501
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 RUVBL1 8607
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 POLR2I 5438
Affinity Capture-MS Homo sapiens
20 PCBD1 5092
Two-hybrid Homo sapiens
21 POLR3G  
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
22 PSMD12 5718
Affinity Capture-MS Homo sapiens
23 POLR1E  
Affinity Capture-MS Homo sapiens
24 EWSR1 2130
Two-hybrid Homo sapiens
25 CRCP  
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 POLR2C 5432
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
27 MRPS7 51081
Co-fractionation Homo sapiens
28 GPN1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 POLR2M  
Affinity Capture-MS Homo sapiens
30 PFDN6 10471
Affinity Capture-MS Homo sapiens
31 SMARCB1 6598
Co-fractionation Homo sapiens
32 NEDD4 4734
Reconstituted Complex Homo sapiens
33 GPN3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 POLR2K  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 POLR1A 25885
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
36 PPP1CA 5499
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 MAF1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 MCC 4163
Affinity Capture-MS Homo sapiens
39 UXT 8409
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 POLR3K  
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 PARK2  
Affinity Capture-MS Homo sapiens
42 PSME1 5720
Affinity Capture-MS Homo sapiens
43 PRPS2 5634
Affinity Capture-MS Homo sapiens
44 TUFM 7284
Co-fractionation Homo sapiens
45 POLR3F  
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
46 BDP1  
Affinity Capture-MS Homo sapiens
47 SMARCA4 6597
Co-fractionation Homo sapiens
48 PFDN2 5202
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 PSMD2 5708
Affinity Capture-MS Homo sapiens
50 NTRK1 4914
Affinity Capture-MS Homo sapiens
51 MRPL11 65003
Co-fractionation Homo sapiens
52 POLR3GL  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
53 VPS18 57617
Co-fractionation Homo sapiens
54 PIPSL 266971
Affinity Capture-MS Homo sapiens
55 IPO9 55705
Co-fractionation Homo sapiens
56 POLR2B 5431
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
57 RPS5 6193
Co-fractionation Homo sapiens
58 GTF3C3 9330
Co-fractionation Homo sapiens
59 POLR2H 5437
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
60 Prkcz  
Affinity Capture-MS Mus musculus
61 EGLN3  
Affinity Capture-MS Homo sapiens
62 SMARCA5 8467
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
63 POLR2E 5434
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
64 RPRD2  
Affinity Capture-MS Homo sapiens
65 RPRD1A  
Affinity Capture-MS Homo sapiens
66 POLR2D  
Affinity Capture-MS Homo sapiens
67 POLR2A 5430
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
68 POLR3E 55718
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
69 TBP  
Affinity Capture-MS Homo sapiens
70 HIST1H1B 3009
Co-fractionation Homo sapiens
71 RPA3 6119
Proximity Label-MS Homo sapiens
72 CSNK2A2 1459
Affinity Capture-MS Homo sapiens
73 ASF1A 25842
Co-localization Homo sapiens
74 PTGES3 10728
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
75 RPAP3 79657
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 WDR92 116143
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
77 HAX1  
Proximity Label-MS Homo sapiens
78 TSR1 55720
Co-fractionation Homo sapiens
79 BRD3 8019
Affinity Capture-MS Homo sapiens
80 C9orf72  
Affinity Capture-MS Homo sapiens
81 RAE1 8480
Affinity Capture-MS Homo sapiens
82 PPP1R15A  
Affinity Capture-MS Homo sapiens
83 FTL 2512
Affinity Capture-MS Homo sapiens
84 HSPE1 3336
Cross-Linking-MS (XL-MS) Homo sapiens
85 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
86 POLR2F  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Affinity Capture-MS Homo sapiens
87 MRPL22  
Co-fractionation Homo sapiens
88 POLR2J  
Affinity Capture-MS Homo sapiens
89 POLR1B 84172
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
90 RUVBL2 10856
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
91 POLR2L 5441
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
92 TRIM52  
Affinity Capture-MS Homo sapiens
93 POLR3C 10623
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
94 POLR1C 9533
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
95 Zw10  
Affinity Capture-MS Mus musculus
96 POLR3H  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
97 RPAP1  
Affinity Capture-MS Homo sapiens
98 IKBKG 8517
Two-hybrid Homo sapiens
99 ASB13  
Affinity Capture-MS Homo sapiens
100 SLC7A6OS  
Affinity Capture-MS Homo sapiens
101 PPP2R2C 5522
Affinity Capture-MS Homo sapiens
102 MRPL2 51069
Co-fractionation Homo sapiens
103 HDAC1 3065
Co-fractionation Homo sapiens
104 DDX58 23586
Affinity Capture-RNA Homo sapiens
105 GTF2B 2959
Affinity Capture-Western Homo sapiens
106 EVA1C  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
107 SKAP1 8631
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
108 POLR3D  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
109 POLR3B 55703
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
110 RPRD1B 58490
Affinity Capture-MS Homo sapiens
111 PIH1D1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
112 ZNRD1  
Affinity Capture-MS Homo sapiens
113 HIRA  
Co-localization Homo sapiens
114 KRAS 3845
Synthetic Lethality Homo sapiens
Negative Genetic Homo sapiens
115 POLR1D 51082
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
116 UBN1  
Co-localization Homo sapiens
117 PSMD3 5709
Affinity Capture-MS Homo sapiens
118 LURAP1  
Affinity Capture-MS Homo sapiens
119 RBP4 5950
Affinity Capture-MS Homo sapiens
120 PDRG1  
Affinity Capture-MS Homo sapiens
121 ASB7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
122 MYL12B 103910
Affinity Capture-MS Homo sapiens
123 Cdc26  
Affinity Capture-MS Mus musculus
124 CAPN2 824
Affinity Capture-MS Homo sapiens
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