Gene description for CAP2
Gene name CAP, adenylate cyclase-associated protein, 2 (yeast)
Gene symbol CAP2
Other names/aliases -
Species Homo sapiens
 Database cross references - CAP2
ExoCarta ExoCarta_10486
Vesiclepedia VP_10486
Entrez Gene 10486
HGNC 20039
UniProt P40123  
 CAP2 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for CAP2
Molecular Function
    actin binding GO:0003779 IBA
    protein binding GO:0005515 IPI
    adenylate cyclase binding GO:0008179 IBA
    identical protein binding GO:0042802 IPI
Biological Process
    cell morphogenesis GO:0000902 IBA
    actin filament organization GO:0007015 IBA
    establishment or maintenance of cell polarity GO:0007163 TAS
    signal transduction GO:0007165 TAS
    activation of adenylate cyclase activity GO:0007190 TAS
    cAMP-mediated signaling GO:0019933 IBA
    presynaptic actin cytoskeleton organization GO:0099140 IEA
    regulation of modification of postsynaptic actin cytoskeleton GO:1905274 IEA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    plasma membrane GO:0005886 IEA
    postsynaptic density GO:0014069 IEA
    glutamatergic synapse GO:0098978 IEA
 Experiment description of studies that identified CAP2 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for CAP2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 TRMT1 55621
Co-fractionation Homo sapiens
3 FASTKD2  
Co-fractionation Homo sapiens
4 TWF2 11344
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 TP53 7157
Affinity Capture-MS Homo sapiens
6 MSH2 4436
Co-fractionation Homo sapiens
7 POTEJ 653781
Affinity Capture-MS Homo sapiens
8 POTEI 653269
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 NCKAP1 10787
Co-fractionation Homo sapiens
10 YWHAE 7531
Two-hybrid Homo sapiens
11 ACTA2 59
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 APEX1 328
Affinity Capture-RNA Homo sapiens
13 CAP1 10487
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
14 ACTC1 70
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 UBA1 7317
Co-fractionation Homo sapiens
16 HSP90AB1 3326
Cross-Linking-MS (XL-MS) Homo sapiens
17 ACTBL2 345651
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 CNRIP1 25927
Affinity Capture-MS Homo sapiens
19 BRD2  
Affinity Capture-MS Homo sapiens
20 UBC 7316
Affinity Capture-MS Homo sapiens
21 EGFR 1956
Affinity Capture-MS Homo sapiens
22 CAP2 10486
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
23 INPP4B 8821
Co-fractionation Homo sapiens
24 MCM4 4173
Co-fractionation Homo sapiens
25 EIF4A1 1973
Co-fractionation Homo sapiens
26 CFL1 1072
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 PRPF40A 55660
Two-hybrid Homo sapiens
28 SUPT5H 6829
Affinity Capture-MS Homo sapiens
29 BRD3 8019
Affinity Capture-MS Homo sapiens
30 POTEF 728378
Affinity Capture-MS Homo sapiens
31 ARHGEF1 9138
Co-fractionation Homo sapiens
32 KIAA1429 25962
Affinity Capture-MS Homo sapiens
33 ADO  
Two-hybrid Homo sapiens
34 CAPZB 832
Affinity Capture-MS Homo sapiens
35 ACTA1 58
Affinity Capture-MS Homo sapiens
36 FKBP4 2288
Co-fractionation Homo sapiens
37 BCL7C  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 NPEPL1 79716
Co-fractionation Homo sapiens
39 CSNK2A2 1459
Co-fractionation Homo sapiens
40 Actb 11461
Affinity Capture-MS Mus musculus
41 MT1P3  
Two-hybrid Homo sapiens
42 GRXCR1  
Two-hybrid Homo sapiens
43 CFL2 1073
Two-hybrid Homo sapiens
44 GBE1 2632
Co-fractionation Homo sapiens
45 AP1G1 164
Co-fractionation Homo sapiens
46 ACTB 60
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 STYX  
Affinity Capture-MS Homo sapiens
48 CCDC40  
Affinity Capture-MS Homo sapiens
49 GOLPH3 64083
Co-fractionation Homo sapiens
50 BCL7A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 ACTG1 71
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
52 AP1S1 1174
Co-fractionation Homo sapiens
View the network image/svg+xml



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