Experiment description of studies that identified IGHA2 in sEVs

1 Experiment ID 494 MISEV standards ✔ EM Biophysical techniques ✔ CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35931686     Organism Homo sapiens Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD." Journal name Cell Death Dis Publication year 2022 Sample Chondrocytes Sample name Osteoarthritic cartilage Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

2 Experiment ID 496 MISEV standards ✘ Biophysical techniques ✔ CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35931686     Organism Homo sapiens Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD." Journal name Cell Death Dis Publication year 2022 Sample Chondrocytes Sample name Healthy cartilage Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

3 Experiment ID 126 MISEV standards ✘ Biophysical techniques ✔ GAPDH Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry [LTQ-FT Ultra] PubMed ID Unpublished / Not applicable Organism Homo sapiens Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy? Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim" Journal name Mesenchymal Stem Cell Therapy Publication year 2011 Sample Mesenchymal stem cells Sample name huES9.E1 Isolation/purification methods HPLC Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Antobody array Mass spectrometry

4 Experiment ID 231 MISEV standards ✘ Biophysical techniques ✔ Alix|CD63|CD9 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25332113     Organism Homo sapiens Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G." Journal name Transfusion Publication year 2015 Sample Platelets Sample name PL-Exs - Rep 1 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient Flotation density 1.12-1.15 g/mL Molecules identified in the study Protein Lipids Methods used in the study Western blotting Mass spectrometry

5 Experiment ID 232 MISEV standards ✘ Biophysical techniques ✘ Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25332113     Organism Homo sapiens Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G." Journal name Transfusion Publication year 2015 Sample Platelets Sample name PL-Exs - Rep 2 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient Flotation density 1.12-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

6 Experiment ID 233 MISEV standards ✘ Biophysical techniques ✘ Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25332113     Organism Homo sapiens Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G." Journal name Transfusion Publication year 2015 Sample Platelets Sample name PL-Exs - Rep 3 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient Flotation density 1.12-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

7 Experiment ID 66 MISEV standards ✔ IEM Biophysical techniques ✔ TSG101|Alix|CD63|CD81 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 19199708     Organism Homo sapiens Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT). Authors "Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR" Journal name JPR Publication year 2009 Sample Saliva Sample name Saliva Isolation/purification methods Differential centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [LTQ] Western blotting Immunoelectron Microscopy

8 Experiment ID 238 MISEV standards ✔ EM Biophysical techniques ✔ TSG101 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25452312     Organism Homo sapiens Experiment description N-linked (N-) glycoproteomics of urimary exosomes Authors "Saraswat M, Joenvaara S, Musante L, Peltoniemi H4, Holthofer H, Renkonen R." Journal name Mol Cell Proteomics Publication year 2014 Sample Urine Sample name Urine - Normal Isolation/purification methods Differential centrifugation Filtration Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

Protein-protein interactions for IGHA2

Protein Interactor ExoCarta ID Identification method PubMed Species 1 PMEPA1 56937 Affinity Capture-MS Homo sapiens 2 MATN2 4147 Affinity Capture-MS Homo sapiens 3 GNG8   Affinity Capture-MS Homo sapiens 4 SNX27 81609 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 5 WDR6 11180 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 6 PLB1   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 7 ATP11C 286410 Affinity Capture-MS Homo sapiens 8 PPP2R2B 5521 Affinity Capture-MS Homo sapiens 9 TWF1 5756 Affinity Capture-MS Homo sapiens 10 UQCRQ 27089 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 11 ZNF695   Affinity Capture-MS Homo sapiens 12 CHAC2 494143 Affinity Capture-MS Homo sapiens 13 SSFA2 6744 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 14 AGPAT1 10554 Affinity Capture-MS Homo sapiens 15 USPL1   Affinity Capture-MS Homo sapiens 16 DDX3X 1654 Affinity Capture-MS Homo sapiens 17 TMEM176B 28959 Affinity Capture-MS Homo sapiens 18 CRYGN   Affinity Capture-MS Homo sapiens 19 GALR3   Affinity Capture-MS Homo sapiens 20 MAGEB4   Affinity Capture-MS Homo sapiens 21 DDX31   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 22 RUNX2   Affinity Capture-MS Homo sapiens 23 KLF15   Affinity Capture-MS Homo sapiens 24 UCP2   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 25 DXO   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 26 PCK2 5106 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 27 PPCS 79717 Affinity Capture-MS Homo sapiens 28 SLC25A15   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 29 CIART   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 30 IGFBP5 3488 Affinity Capture-MS Homo sapiens 31 EBF2   Affinity Capture-MS Homo sapiens 32 NUAK1   Affinity Capture-MS Homo sapiens 33 SPNS3   Affinity Capture-MS Homo sapiens 34 SNF8 11267 Affinity Capture-MS Homo sapiens 35 DNAH17 8632 Affinity Capture-MS Homo sapiens 36 FNDC5   Affinity Capture-MS Homo sapiens 37 FKBP14   Affinity Capture-MS Homo sapiens 38 HBQ1   Affinity Capture-MS Homo sapiens 39 GOLGA8A 23015 Affinity Capture-MS Homo sapiens 40 COG2 22796 Affinity Capture-MS Homo sapiens 41 USB1   Affinity Capture-MS Homo sapiens 42 SRRT 51593 Affinity Capture-MS Homo sapiens 43 KIR2DS2   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 44 FKBP5 2289 Affinity Capture-MS Homo sapiens 45 FRMD1   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 46 MUC5AC 4586 Affinity Capture-MS Homo sapiens 47 BTBD1   Affinity Capture-MS Homo sapiens 48 TMEM257   Affinity Capture-MS Homo sapiens 49 TMEM237 65062 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 50 ZNF491   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 51 CDK14 5218 Affinity Capture-MS Homo sapiens 52 SCAMP2 10066 Affinity Capture-MS Homo sapiens 53 ITLN2   Affinity Capture-MS Homo sapiens 54 RIBC1   Affinity Capture-MS Homo sapiens 55 HBM   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 56 RHOBTB1   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 57 CDK2AP1   Affinity Capture-MS Homo sapiens 58 GLRX 2745 Affinity Capture-MS Homo sapiens 59 LRRC8E 80131 Affinity Capture-MS Homo sapiens 60 STK11 6794 Affinity Capture-MS Homo sapiens 61 HINT2 84681 Affinity Capture-MS Homo sapiens 62 PRKX 5613 Affinity Capture-MS Homo sapiens 63 SNW1 22938 Affinity Capture-MS Homo sapiens 64 RHBDD1 84236 Affinity Capture-MS Homo sapiens 65 TIMM10   Affinity Capture-MS Homo sapiens 66 DDX17 10521 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 67 APCDD1   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 68 OR13C3   Affinity Capture-MS Homo sapiens 69 NPB   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 70 COMMD1 150684 Affinity Capture-MS Homo sapiens 71 TSGA13   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 72 JAK2 3717 Affinity Capture-MS Homo sapiens 73 SFXN4   Affinity Capture-MS Homo sapiens 74 BRD3 8019 Affinity Capture-MS Homo sapiens 75 GJB5   Affinity Capture-MS Homo sapiens 76 NMUR2   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 77 CCR1   Affinity Capture-MS Homo sapiens 78 NXT1   Affinity Capture-MS Homo sapiens 79 TCEB3B   Affinity Capture-MS Homo sapiens 80 PHF11 51131 Affinity Capture-MS Homo sapiens View the network

Pathways in which IGHA2 is involved

No pathways found