Gene description for RASAL3
Gene name RAS protein activator like 3
Gene symbol RASAL3
Other names/aliases -
Species Homo sapiens
 Database cross references - RASAL3
ExoCarta ExoCarta_64926
Vesiclepedia VP_64926
Entrez Gene 64926
HGNC 26129
UniProt Q86YV0  
 RASAL3 identified in exosomes derived from the following tissue/cell type
B cells 20458337    
B cells 20458337    
B cells 20458337    
Thymus 23844026    
 Gene ontology annotations for RASAL3
Molecular Function
    GTPase activator activity GO:0005096 IEA
    protein binding GO:0005515 IPI
    identical protein binding GO:0042802 IPI
Biological Process
    negative regulation of Ras protein signal transduction GO:0046580 IMP
    positive regulation of NK T cell proliferation GO:0051142 IMP
Subcellular Localization
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
    cell cortex GO:0005938 IEA
    membrane GO:0016020 HDA
    extracellular exosome GO:0070062 HDA
    cytoplasmic side of membrane GO:0098562 IDA
 Experiment description of studies that identified RASAL3 in exosomes
1
Experiment ID 79
MISEV standards
✘ Biophysical techniques
✔
CD81|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
2
Experiment ID 80
MISEV standards
✘ Biophysical techniques
✔
CD81|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
3
Experiment ID 81
MISEV standards
✘ Biophysical techniques
✔
CD81|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
4
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for RASAL3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPK 3190
Two-hybrid Homo sapiens
2 BEGAIN  
Two-hybrid Homo sapiens
3 RABGEF1 27342
Two-hybrid Homo sapiens
4 YWHAE 7531
Two-hybrid Homo sapiens
5 HOMER1  
Two-hybrid Homo sapiens
6 PRKAA2 5563
Two-hybrid Homo sapiens
7 CCDC136  
Two-hybrid Homo sapiens
8 KHDRBS3  
Two-hybrid Homo sapiens
9 KRT27 342574
Two-hybrid Homo sapiens
10 YWHAZ 7534
Two-hybrid Homo sapiens
11 MTUS2 23281
Two-hybrid Homo sapiens
12 WASF3 10810
Two-hybrid Homo sapiens
13 AMOTL2 51421
Two-hybrid Homo sapiens
14 BRD3 8019
Affinity Capture-MS Homo sapiens
15 RASD1  
Two-hybrid Homo sapiens
16 TP53BP2  
Two-hybrid Homo sapiens
17 CCDC102B  
Two-hybrid Homo sapiens
18 RASAL3 64926
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
19 DDX58 23586
Affinity Capture-RNA Homo sapiens
20 CDR2L  
Two-hybrid Homo sapiens
21 WDYHV1  
Two-hybrid Homo sapiens
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