Gene description for MAP1LC3B
Gene name microtubule-associated protein 1 light chain 3 beta
Gene symbol MAP1LC3B
Other names/aliases ATG8F
LC3B
MAP1A/1BLC3
MAP1LC3B-a
Species Homo sapiens
 Database cross references - MAP1LC3B
ExoCarta ExoCarta_81631
Vesiclepedia VP_81631
Entrez Gene 81631
HGNC 13352
MIM 609604
UniProt Q9GZQ8  
 MAP1LC3B identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Endothelial cells 26027894    
 Gene ontology annotations for MAP1LC3B
Molecular Function
    protein binding GO:0005515 IPI
    microtubule binding GO:0008017 IBA
    phosphatidylethanolamine binding GO:0008429 IBA
    phosphatidylethanolamine binding GO:0008429 IDA
    ubiquitin protein ligase binding GO:0031625 IBA
    ubiquitin protein ligase binding GO:0031625 IPI
    ceramide binding GO:0097001 IDA
Biological Process
    autophagosome assembly GO:0000045 IBA
    autophagosome assembly GO:0000045 IDA
    mitophagy GO:0000423 IBA
    mitophagy GO:0000423 IDA
    mitophagy GO:0000423 IGI
    autophagy GO:0006914 IDA
    cellular response to nitrogen starvation GO:0006995 IBA
    cellular response to starvation GO:0009267 IDA
    macroautophagy GO:0016236 IDA
    autophagosome maturation GO:0097352 IBA
    autophagosome maturation GO:0097352 IDA
Subcellular Localization
    autophagosome membrane GO:0000421 IBA
    autophagosome membrane GO:0000421 IDA
    autophagosome membrane GO:0000421 TAS
    mitochondrion GO:0005739 IDA
    autophagosome GO:0005776 IDA
    autophagosome GO:0005776 ISS
    cytosol GO:0005829 TAS
    microtubule GO:0005874 IEA
    axoneme GO:0005930 ISS
    endomembrane system GO:0012505 IEA
    organelle membrane GO:0031090 ISS
    cytoplasmic vesicle GO:0031410 IDA
    mitochondrial membrane GO:0031966 IEA
    intracellular membrane-bounded organelle GO:0043231 IDA
 Experiment description of studies that identified MAP1LC3B in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 226
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD9|FLOT1
Enriched markers
✔
LMNA|H2AFX|ATP5A1|TOMM20
Negative markers
✘ Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description "Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting"
Authors "Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar"
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
 Protein-protein interactions for MAP1LC3B
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RAP1GAP 5909
Two-hybrid Homo sapiens
2 GABARAPL2 11345
Reconstituted Complex Homo sapiens
3 TNIP1 10318
Two-hybrid Homo sapiens
4 CCSER2 54462
Two-hybrid Homo sapiens
5 CCT3 7203
Affinity Capture-Western Homo sapiens
6 ATG5 9474
Affinity Capture-MS Homo sapiens
7 GJA1 2697
Affinity Capture-Western Homo sapiens
8 MTX1 4580
Reconstituted Complex Homo sapiens
9 GJA4  
Affinity Capture-Western Homo sapiens
10 ADRB2  
Affinity Capture-Western Homo sapiens
11 DBN1 1627
Two-hybrid Homo sapiens
12 ATG101  
Affinity Capture-Western Homo sapiens
13 CALCOCO1 57658
Affinity Capture-Western Homo sapiens
14 EPS8 2059
Two-hybrid Homo sapiens
15 BNIP3L  
Affinity Capture-Western Homo sapiens
16 NHP2L1 4809
Two-hybrid Homo sapiens
17 VHL  
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
18 TAF7  
Two-hybrid Homo sapiens
19 ATG4C  
Reconstituted Complex Homo sapiens
20 ATG13 9776
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
21 SAFB 6294
Two-hybrid Homo sapiens
22 Cacna1b  
Affinity Capture-Western Rattus norvegicus
23 SPAG9 9043
Two-hybrid Homo sapiens
24 SRC 6714
Affinity Capture-Western Homo sapiens
25 FAM65A 79567
Two-hybrid Homo sapiens
26 CALCOCO2  
Affinity Capture-Western Homo sapiens
27 HADHA 3030
Affinity Capture-MS Homo sapiens
28 RASSF5 83593
Affinity Capture-MS Homo sapiens
29 TMEM192 201931
Affinity Capture-MS Homo sapiens
30 EED  
Two-hybrid Homo sapiens
31 ATG7 10533
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
32 ATG4D  
Reconstituted Complex Homo sapiens
33 ATG16L1 55054
Affinity Capture-MS Homo sapiens
34 ADD1 118
Two-hybrid Homo sapiens
35 DOCK1 1793
Affinity Capture-MS Homo sapiens
36 SMARCB1 6598
Two-hybrid Homo sapiens
37 NEDD4 4734
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
38 SLC25A3 5250
Cross-Linking-MS (XL-MS) Homo sapiens
39 CCT2 10576
Affinity Capture-Western Homo sapiens
40 TBC1D17 79735
Two-hybrid Homo sapiens
41 MTCH2 23788
Affinity Capture-Western Homo sapiens
42 ATG4A  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
43 FOXA1  
Affinity Capture-MS Homo sapiens
44 SAMM50 25813
Reconstituted Complex Homo sapiens
45 USP10 9100
Biochemical Activity Homo sapiens
46 RB1CC1 9821
Affinity Capture-Western Homo sapiens
47 MAP1B 4131
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 AMFR 267
Affinity Capture-MS Homo sapiens
49 NBR1 4077
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
50 OPTN 10133
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Protein-peptide Homo sapiens
Co-localization Homo sapiens
Protein-peptide Homo sapiens
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
Affinity Capture-Western Homo sapiens
51 FUNDC1  
Two-hybrid Homo sapiens
52 MAPT  
Co-fractionation Homo sapiens
53 MOAP1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
54 GABARAP 11337
Affinity Capture-MS Homo sapiens
55 TRIM21 6737
Reconstituted Complex Homo sapiens
56 GORASP2 26003
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
57 FAM134A 79137
Two-hybrid Homo sapiens
58 TBC1D5 9779
Affinity Capture-Western Homo sapiens
59 MYBL2 4605
Two-hybrid Homo sapiens
60 ULK1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
61 RPLP1 6176
Two-hybrid Homo sapiens
62 PML 5371
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
63 NIPSNAP1 8508
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
64 ARFGAP1 55738
Two-hybrid Homo sapiens
65 IRGQ 126298
Two-hybrid Homo sapiens
66 VCP 7415
Affinity Capture-Western Homo sapiens
67 MESDC2 23184
Two-hybrid Homo sapiens
68 GJA8  
Affinity Capture-Western Homo sapiens
69 STK3 6788
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
70 DBI 1622
Two-hybrid Homo sapiens
71 HADHB 3032
Affinity Capture-MS Homo sapiens
72 CLINT1 9685
Affinity Capture-MS Homo sapiens
73 GJA5 2702
Affinity Capture-Western Homo sapiens
74 TAX1BP1 8887
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
75 AMBRA1  
Affinity Capture-Western Homo sapiens
Protein-peptide Homo sapiens
76 CCT6A 908
Affinity Capture-Western Homo sapiens
77 UBE2L3 7332
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
78 LAMP1 3916
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
79 PRKCZ 5590
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
80 SNX18 112574
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
81 TMEM160 54958
Affinity Capture-MS Homo sapiens
82 ADNP 23394
Two-hybrid Homo sapiens
83 GJA3  
Affinity Capture-Western Homo sapiens
84 KBTBD7  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
85 PRKCI 5584
Affinity Capture-MS Homo sapiens
86 LUC7L2 51631
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
87 DNMT3B  
Two-hybrid Homo sapiens
88 STAT1 6772
Co-fractionation Homo sapiens
89 RPA3 6119
Proximity Label-MS Homo sapiens
90 KLHL5  
Two-hybrid Homo sapiens
91 MAPK15  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
92 TMOD3 29766
Two-hybrid Homo sapiens
93 MRFAP1  
Two-hybrid Homo sapiens
94 USP19 10869
Affinity Capture-Western Homo sapiens
95 CACNA1B 774
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
96 BNIP3 664
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Reconstituted Complex Homo sapiens
97 STK4 6789
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
98 TBC1D9B 23061
Two-hybrid Homo sapiens
99 UPF2 26019
Two-hybrid Homo sapiens
100 BRD3 8019
Two-hybrid Homo sapiens
101 IRF3 3661
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
102 TOMM20 9804
Co-localization Homo sapiens
103 Cacnb3  
Affinity Capture-Western Rattus norvegicus
104 TBC1D25  
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
105 GBAS 2631
Affinity Capture-MS Homo sapiens
106 MKRN1 23608
Two-hybrid Homo sapiens
107 TP53 7157
Affinity Capture-Western Homo sapiens
108 TBC1D9  
Two-hybrid Homo sapiens
109 KEAP1 9817
Affinity Capture-MS Homo sapiens
110 CASP8 841
Two-hybrid Homo sapiens
111 SQSTM1 8878
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Protein-peptide Homo sapiens
112 SHFM1 7979
Two-hybrid Homo sapiens
113 FYCO1 79443
Affinity Capture-MS Homo sapiens
Protein-peptide Homo sapiens
Affinity Capture-MS Homo sapiens
114 HDAC1 3065
Synthetic Rescue Homo sapiens
115 CDK1 983
Affinity Capture-Western Homo sapiens
116 FLCN 201163
Reconstituted Complex Homo sapiens
117 THAP7  
Two-hybrid Homo sapiens
118 TBK1 29110
Reconstituted Complex Homo sapiens
119 MAP1LC3A 84557
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
120 ATG4B 23192
Biochemical Activity Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
121 PINK1  
Affinity Capture-Western Homo sapiens
122 CLTA 1211
Two-hybrid Homo sapiens
123 KXD1 79036
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
124 MAP1A 4130
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
125 NEK9 91754
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
126 EVA1A 84141
Reconstituted Complex Homo sapiens
127 AAMP 14
Two-hybrid Homo sapiens
128 NGB  
Affinity Capture-Western Homo sapiens
129 ATG3 64422
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
130 TCP1 6950
Affinity Capture-Western Homo sapiens
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