Gene description for PIR
Gene name pirin (iron-binding nuclear protein)
Gene symbol PIR
Other names/aliases -
Species Homo sapiens
 Database cross references - PIR
ExoCarta ExoCarta_8544
Vesiclepedia VP_8544
Entrez Gene 8544
HGNC 30048
MIM 300931
UniProt O00625  
 PIR identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for PIR
Molecular Function
    transcription coregulator activity GO:0003712 IMP
    protein binding GO:0005515 IPI
    quercetin 2,3-dioxygenase activity GO:0008127 IBA
    quercetin 2,3-dioxygenase activity GO:0008127 IDA
    quercetin 2,3-dioxygenase activity GO:0008127 TAS
    metal ion binding GO:0046872 IDA
Biological Process
    transcription by RNA polymerase II GO:0006366 TAS
    digestion GO:0007586 TAS
    monocyte differentiation GO:0030224 IBA
    monocyte differentiation GO:0030224 IMP
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IDA
    nuclear body GO:0016604 IDA
 Experiment description of studies that identified PIR in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔
Alix|CD81|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PIR
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NIT2 56954
Co-fractionation Homo sapiens
2 PSMB2 5690
Co-fractionation Homo sapiens
3 KLHL20  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 NME2 4831
Co-fractionation Homo sapiens
5 NANS 54187
Co-fractionation Homo sapiens
6 SELENBP1 8991
Co-fractionation Homo sapiens
7 HTRA2 27429
Co-fractionation Homo sapiens
8 SNX3 8724
Co-fractionation Homo sapiens
9 DCXR 51181
Co-fractionation Homo sapiens
10 SOD2 6648
Co-fractionation Homo sapiens
11 TIA1 7072
Co-fractionation Homo sapiens
12 TIAL1 7073
Co-fractionation Homo sapiens
13 NFIX 4784
Two-hybrid Homo sapiens
14 ASS1 445
Co-fractionation Homo sapiens
15 SIN3A  
Co-fractionation Homo sapiens
16 C11orf54 28970
Co-fractionation Homo sapiens
17 C2orf68  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 RIN3  
Affinity Capture-MS Homo sapiens
19 SUGT1 10910
Co-fractionation Homo sapiens
20 SRXN1 140809
Co-fractionation Homo sapiens
21 PFN1 5216
Co-fractionation Homo sapiens
22 UBE2L3 7332
Co-fractionation Homo sapiens
23 GRHPR 9380
Co-fractionation Homo sapiens
24 NFKBIA  
Two-hybrid Homo sapiens
25 ANXA7 310
Co-fractionation Homo sapiens
26 ACAT2 39
Co-fractionation Homo sapiens
27 PTGR1 22949
Co-fractionation Homo sapiens
28 MT1X 4501
Co-fractionation Homo sapiens
29 FGB 2244
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 ECI1 1632
Cross-Linking-MS (XL-MS) Homo sapiens
31 TKT 7086
Co-fractionation Homo sapiens
32 BCL3  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
33 FAHD1 81889
Co-fractionation Homo sapiens
34 PUF60 22827
Affinity Capture-MS Homo sapiens
35 PDCD6IP 10015
Co-fractionation Homo sapiens
36 PARK7 11315
Co-fractionation Homo sapiens
37 CLNS1A 1207
Co-fractionation Homo sapiens
38 CFL2 1073
Co-fractionation Homo sapiens
39 PMM2 5373
Co-fractionation Homo sapiens
40 NXF1 10482
Affinity Capture-RNA Homo sapiens
41 ARHGDIA 396
Co-fractionation Homo sapiens
42 ADK 132
Co-fractionation Homo sapiens
43 MIF 4282
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
44 ANXA11 311
Co-fractionation Homo sapiens
45 SEC14L2  
Co-fractionation Homo sapiens
46 PPIA 5478
Co-fractionation Homo sapiens
47 CAPG 822
Co-fractionation Homo sapiens
48 PEBP1 5037
Co-fractionation Homo sapiens
49 SPEN 23013
Co-fractionation Homo sapiens
50 DSTN 11034
Co-fractionation Homo sapiens
51 SMAD9  
Two-hybrid Homo sapiens
52 GNPDA2 132789
Co-fractionation Homo sapiens
53 FKBP1A 2280
Co-fractionation Homo sapiens
54 DAZAP1 26528
Co-fractionation Homo sapiens
55 GOT1 2805
Co-fractionation Homo sapiens
56 CFL1 1072
Co-fractionation Homo sapiens
57 ACO2 50
Co-fractionation Homo sapiens
58 PPP2CA 5515
Co-fractionation Homo sapiens
59 UBL7  
Co-fractionation Homo sapiens
60 FH 2271
Co-fractionation Homo sapiens
61 HMGCL 3155
Co-fractionation Homo sapiens
62 HSPE1 3336
Co-fractionation Homo sapiens
63 GNB2L1 10399
Co-fractionation Homo sapiens
64 ANXA1 301
Co-fractionation Homo sapiens
65 TKFC 26007
Co-fractionation Homo sapiens
66 CRYZ 1429
Co-fractionation Homo sapiens
67 FUBP1 8880
Co-fractionation Homo sapiens
68 GNPDA1 10007
Co-fractionation Homo sapiens
69 FAHD2A 51011
Co-fractionation Homo sapiens
70 SOD1 6647
Co-fractionation Homo sapiens
71 SRI 6717
Co-fractionation Homo sapiens
72 STAM 8027
Co-fractionation Homo sapiens
73 GSR 2936
Co-fractionation Homo sapiens
View the network image/svg+xml
 Pathways in which PIR is involved
PathwayEvidenceSource
Digestion TAS Reactome
Digestion and absorption TAS Reactome





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