Gene description for PPM1B
Gene name protein phosphatase, Mg2+/Mn2+ dependent, 1B
Gene symbol PPM1B
Other names/aliases PP2C-beta
PP2C-beta-X
PP2CB
PP2CBETA
PPC2BETAX
Species Homo sapiens
 Database cross references - PPM1B
ExoCarta ExoCarta_5495
Entrez Gene 5495
HGNC 9276
MIM 603770
UniProt O75688  
 PPM1B identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for PPM1B
Molecular Function
    protein binding GO:0005515 IPI
    manganese ion binding GO:0030145 IEA
    protein serine/threonine phosphatase activity GO:0004722 IDA
    magnesium ion binding GO:0000287 IEA
Biological Process
    negative regulation of defense response to virus GO:0050687 IMP
    N-terminal protein myristoylation GO:0006499 ISS
    peptidyl-threonine dephosphorylation GO:0035970 IDA
    protein dephosphorylation GO:0006470 IMP
    negative regulation of interferon-beta production GO:0032688 IMP
    cytokine-mediated signaling pathway GO:0019221 TAS
    negative regulation of NF-kappaB import into nucleus GO:0042347 IMP
    negative regulation of I-kappaB kinase/NF-kappaB signaling GO:0043124 IMP
Subcellular Localization
    cytosol GO:0005829 TAS
    membrane GO:0016020 ISS
 Experiment description of studies that identified PPM1B in exosomes
1
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PPM1B
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PPP2CA 5515
Co-purification Homo sapiens
2 GSN 2934
Affinity Capture-MS Homo sapiens
3 MAP3K7  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
4 KRT18 3875
Affinity Capture-MS Homo sapiens
5 ANXA1 301
Affinity Capture-MS Homo sapiens
6 CDK2 1017
Biochemical Activity Homo sapiens
Co-purification Homo sapiens
7 IKBKG 8517
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
8 ANXA2 302
Affinity Capture-MS Homo sapiens
9 S100A8 6279
Affinity Capture-MS Homo sapiens
10 VIL1 7429
Affinity Capture-MS Homo sapiens
11 IKBKB 3551
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
12 CHUK 1147
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
13 VCP 7415
Affinity Capture-MS Homo sapiens
14 PABPC4 8761
Affinity Capture-MS Homo sapiens
15 CDK6 1021
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
Co-purification Homo sapiens
16 RASGRP1  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which PPM1B is involved
PathwayEvidenceSource
ISG15 antiviral mechanism TAS Reactome





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