Gene description for CLNS1A
Gene name chloride channel, nucleotide-sensitive, 1A
Gene symbol CLNS1A
Other names/aliases CLCI
CLNS1B
ICln
Species Homo sapiens
 Database cross references - CLNS1A
ExoCarta ExoCarta_1207
Entrez Gene 1207
HGNC 2080
MIM 602158
UniProt P54105  
 CLNS1A identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for CLNS1A
Molecular Function
    poly(A) RNA binding GO:0044822 IDA
    protein heterodimerization activity GO:0046982 IDA
    protein binding GO:0005515 IPI
Biological Process
    gene expression GO:0010467 TAS
    spliceosomal snRNP assembly GO:0000387 TAS
    chloride transport GO:0006821 IEA
    cell volume homeostasis GO:0006884 IEA
    ncRNA metabolic process GO:0034660 TAS
Subcellular Localization
    cytoplasm GO:0005737 IDA
    nucleus GO:0005634 IDA
    methylosome GO:0034709 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IEA
    pICln-Sm protein complex GO:0034715 IDA
    cytoskeleton GO:0005856 IEA
    nucleoplasm GO:0005654 IDA
 Experiment description of studies that identified CLNS1A in exosomes
1
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for CLNS1A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SNRPB 6628
Affinity Capture-Western Homo sapiens
2 PRMT5 10419
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
3 ITGA2B 3674
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
4 SNRPD1 6632
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
5 SNRPE 6635
Affinity Capture-Western Homo sapiens
6 WDR77 79084
Affinity Capture-MS Homo sapiens
7 SNRPD3 6634
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
8 SNRPD2 6633
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which CLNS1A is involved
PathwayEvidenceSource
snRNP Assembly TAS Reactome





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