Gene description for ISYNA1
Gene name inositol-3-phosphate synthase 1
Gene symbol ISYNA1
Other names/aliases INO1
INOS
IPS
IPS 1
IPS-1
Species Homo sapiens
 Database cross references - ISYNA1
ExoCarta ExoCarta_51477
Entrez Gene 51477
HGNC 29821
MIM 611670
UniProt Q9NPH2  
 ISYNA1 identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for ISYNA1
Molecular Function
    inositol-3-phosphate synthase activity GO:0004512 TAS
    protein binding GO:0005515 IPI
Biological Process
    inositol biosynthetic process GO:0006021 IEA
    phospholipid biosynthetic process GO:0008654 IEA
    small molecule metabolic process GO:0044281 TAS
    inositol phosphate metabolic process GO:0043647 TAS
Subcellular Localization
    cytosol GO:0005829 TAS
 Experiment description of studies that identified ISYNA1 in exosomes
1
Experiment ID 237
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
2
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ISYNA1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PPP2CA 5515
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which ISYNA1 is involved
PathwayEvidenceSource
Synthesis of IP2, IP, and Ins in the cytosol TAS Reactome





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