Gene description for IGFBP5
Gene name insulin-like growth factor binding protein 5
Gene symbol IGFBP5
Other names/aliases IBP5
Species Homo sapiens
 Database cross references - IGFBP5
ExoCarta ExoCarta_3488
Entrez Gene 3488
HGNC 5474
MIM 146734
UniProt P24593  
 IGFBP5 identified in exosomes derived from the following tissue/cell type
Endothelial cells 26027894    
 Gene ontology annotations for IGFBP5
Molecular Function
    protein binding GO:0005515 IPI
    fibronectin binding GO:0001968 IBA
    insulin-like growth factor I binding GO:0031994 IPI
    insulin-like growth factor II binding GO:0031995 IBA
Biological Process
    cellular response to cAMP GO:0071320 IDA
    regulation of glucose metabolic process GO:0010906 IEA
    type B pancreatic cell proliferation GO:0044342 IEA
    osteoblast differentiation GO:0001649 IEA
    intracellular signal transduction GO:0035556 IEA
    negative regulation of translation GO:0017148 IDA
    mammary gland involution GO:0060056 IEA
    negative regulation of smooth muscle cell proliferation GO:0048662 IDA
    cellular response to organic cyclic compound GO:0071407 IDA
    positive regulation of protein kinase B signaling GO:0051897 IEA
    response to growth hormone GO:0060416 ISS
    signal transduction GO:0007165 NAS
    negative regulation of insulin-like growth factor receptor signaling pathway GO:0043569 IDA
    negative regulation of osteoblast differentiation GO:0045668 IEA
    negative regulation of smooth muscle cell migration GO:0014912 IDA
    regulation of cell growth GO:0001558 IEA
    positive regulation of insulin-like growth factor receptor signaling pathway GO:0043568 IEA
    glucose metabolic process GO:0006006 IEA
    glucose homeostasis GO:0042593 IEA
    cellular protein metabolic process GO:0044267 TAS
    skeletal muscle tissue growth GO:0048630 IEA
    hair follicle morphogenesis GO:0031069 IEA
    striated muscle cell differentiation GO:0051146 IEA
    negative regulation of cell migration GO:0030336 IDA
Subcellular Localization
    extracellular space GO:0005615 IBA
    extracellular region GO:0005576 TAS
    intracellular GO:0005622 IEA
    insulin-like growth factor binding protein complex GO:0016942 IC
 Experiment description of studies that identified IGFBP5 in exosomes
1
Experiment ID 226
ISEV standards
EM
EV Biophysical techniques
GAPDH
EV Cytosolic markers
CD9|FLOT1
EV Membrane markers
LMNA|H2AFX|ATP5A1|TOMM20
EV Negative markers
EV Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting
Authors Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
 Protein-protein interactions for IGFBP5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 VTN 7448
Invivo Homo sapiens
2 THBS1 7057
Reconstituted Complex Homo sapiens
3 TF 7018
Invitro Homo sapiens
4 BAG6 7917
Two-hybrid Homo sapiens
5 FHL2  
Invitro Homo sapiens
Invivo Homo sapiens
Two-hybrid Homo sapiens
6 KPNB1 3837
Invitro Homo sapiens
7 SPP1 6696
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
8 IGF1  
Invitro Homo sapiens
Invivo Homo sapiens
9 ADAM12  
Biochemical Activity Homo sapiens
10 IGFALS 3483
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
11 SERPINE1 5054
Invitro Homo sapiens
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
12 IGF2 3481
Affinity Capture-MS Homo sapiens
Invitro Homo sapiens
Invivo Homo sapiens
View the network image/svg+xml



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