ExoCarta: Gene summary

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Gene description for ENSA
Gene name	endosulfine alpha
Gene symbol	ENSA
Other names/aliases	ARPP-19e
Species	Homo sapiens
Database cross references - ENSA
ExoCarta	ExoCarta_2029
Vesiclepedia	VP_2029
Entrez Gene	2029
HGNC	3360
MIM	603061
UniProt	O43768
ENSA identified in sEVs derived from the following tissue/cell type
Breast cancer cells	34108659
Breast cancer cells	34108659
Embryonic kidney cells	34108659
Embryonic kidney cells	34108659
Pancreatic cancer cells	34108659
Pancreatic cancer cells	34108659

Gene ontology annotations for ENSA

Molecular Function
	protein phosphatase inhibitor activity	GO:0004864	IBA
	signaling receptor binding	GO:0005102	IDA
	protein binding	GO:0005515	IPI
	ion channel inhibitor activity	GO:0008200	TAS
	phosphatase inhibitor activity	GO:0019212	ISS
	potassium channel inhibitor activity	GO:0019870	IDA
	protein phosphatase regulator activity	GO:0019888	ISS
	protein phosphatase 2A binding	GO:0051721	ISS
Biological Process
	G2/M transition of mitotic cell cycle	GO:0000086	ISS
	mitotic cell cycle	GO:0000278	ISS
	response to nutrient	GO:0007584	TAS
	regulation of insulin secretion	GO:0050796	IDA
	cell division	GO:0051301	IEA
Subcellular Localization
	nucleoplasm	GO:0005654	TAS
	cytoplasm	GO:0005737	IBA

Experiment description of studies that identified ENSA in sEVs

Experiment ID

426

MISEV standards
✘	Biophysical techniques
✔ SDCBP\|FLOT1\|CD81\|CD9\|CD151\|FLOT2\|TSG101\|LAMP1\|TFRC\|RAB5A\|RAB35\|GAPDH\|UCHL1\|ICAM1	Enriched markers
✔ CANX	Negative markers
✘	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

34108659

Organism

Homo sapiens

Experiment description

Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker

Authors

"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."

Journal name

Nat Cell Biol

Publication year

2021

Sample

Breast cancer cells

Sample name

MDA-MB-231 - Exo-rich fractions 7-10 pooled

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectrometry

Experiment ID

427

MISEV standards
✘	Biophysical techniques
✔ CD81\|SDCBP\|FLOT1\|CD9\|CD151\|FLOT2\|TSG101\|LAMP1\|TFRC\|RAB5A\|RAB35\|GAPDH\|UCHL1\|ICAM1	Enriched markers
✔ CANX	Negative markers
✘	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

34108659

Organism

Homo sapiens

Experiment description

Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker

Authors

"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."

Journal name

Nat Cell Biol

Publication year

2021

Sample

Breast cancer cells

Sample name

MDA-MB-231 - Exo-rich fractions 1-6 pooled

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Mass spectrometry

Experiment ID

419

MISEV standards
✘	Biophysical techniques
✔ SDCBP\|FLOT1\|CD81\|CD9\|CD151\|FLOT2\|TSG101\|LAMP1\|TFRC\|RAB5A\|RAB35\|GAPDH\|UCHL1\|ICAM1	Enriched markers
✔ CANX	Negative markers
✘	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

34108659

Organism

Homo sapiens

Experiment description

Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker

Authors

"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."

Journal name

Nat Cell Biol

Publication year

2021

Sample

Embryonic kidney cells

Sample name

HEK293T - Exo-rich fractions 7-10 pooled

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectrometry

Experiment ID

419

MISEV standards
✘	Biophysical techniques
✔ SDCBP\|FLOT1\|CD81\|CD9\|CD151\|FLOT2\|TSG101\|LAMP1\|TFRC\|RAB5A\|RAB35\|GAPDH\|UCHL1\|ICAM1	Enriched markers
✔ CANX	Negative markers
✘	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

34108659

Organism

Homo sapiens

Experiment description

Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker

Authors

"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."

Journal name

Nat Cell Biol

Publication year

2021

Sample

Embryonic kidney cells

Sample name

HEK293T - Exo-rich fractions 7-10 pooled

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectrometry

Experiment ID

420

MISEV standards
✘	Biophysical techniques
✔ CD81\|SDCBP\|FLOT1\|CD9\|CD151\|FLOT2\|TSG101\|LAMP1\|TFRC\|RAB5A\|RAB35\|GAPDH\|UCHL1\|ICAM1	Enriched markers
✔ CANX	Negative markers
✘	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

34108659

Organism

Homo sapiens

Experiment description

Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker

Authors

"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."

Journal name

Nat Cell Biol

Publication year

2021

Sample

Embryonic kidney cells

Sample name

HEK293T - Exo-rich fractions 1-6 pooled

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Mass spectrometry

Experiment ID

420

MISEV standards
✘	Biophysical techniques
✔ CD81\|SDCBP\|FLOT1\|CD9\|CD151\|FLOT2\|TSG101\|LAMP1\|TFRC\|RAB5A\|RAB35\|GAPDH\|UCHL1\|ICAM1	Enriched markers
✔ CANX	Negative markers
✘	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

34108659

Organism

Homo sapiens

Experiment description

Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker

Authors

"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."

Journal name

Nat Cell Biol

Publication year

2021

Sample

Embryonic kidney cells

Sample name

HEK293T - Exo-rich fractions 1-6 pooled

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Mass spectrometry

Experiment ID

434

MISEV standards
✘	Biophysical techniques
✔ SDCBP\|FLOT1\|CD81\|CD9\|CD151\|FLOT2\|TSG101\|LAMP1\|TFRC\|RAB5A\|RAB35\|GAPDH\|UCHL1\|ICAM1	Enriched markers
✔ CANX	Negative markers
✘	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

34108659

Organism

Homo sapiens

Experiment description

Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker

Authors

"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."

Journal name

Nat Cell Biol

Publication year

2021

Sample

Pancreatic cancer cells

Sample name

PANC-1 - Exo-rich fractions 7-10 pooled

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectrometry

Experiment ID

435

MISEV standards
✘	Biophysical techniques
✔ CD81\|SDCBP\|FLOT1\|CD9\|CD151\|FLOT2\|TSG101\|LAMP1\|TFRC\|RAB5A\|RAB35\|GAPDH\|UCHL1\|ICAM1	Enriched markers
✔ CANX	Negative markers
✘	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

34108659

Organism

Homo sapiens

Experiment description

Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker

Authors

"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."

Journal name

Nat Cell Biol

Publication year

2021

Sample

Pancreatic cancer cells

Sample name

PANC-1 - Exo-rich fractions 1-6 pooled

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Mass spectrometry

Protein-protein interactions for ENSA

Protein Interactor

ExoCarta ID

Identification method

PubMed

Species

ACD

Two-hybrid

Homo sapiens

HNRNPH1

3187

Affinity Capture-RNA

Homo sapiens

TES

26136

Co-fractionation

Homo sapiens

MCM3

4172

Affinity Capture-MS

Homo sapiens

SNCA

6622

Protein-peptide		Homo sapiens
Reconstituted Complex		Homo sapiens
Reconstituted Complex		Homo sapiens

TPPP2

Affinity Capture-MS

Homo sapiens

ARIH2

10425

Two-hybrid

Homo sapiens

ZBTB2

57621

Affinity Capture-MS

Homo sapiens

DPP9

91039

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

QSOX1

5768

Affinity Capture-MS

Homo sapiens

PSEN1

5663

Two-hybrid

Homo sapiens

POT1

Two-hybrid

Homo sapiens

ATP5I

521

Cross-Linking-MS (XL-MS)

Homo sapiens

UBA5

79876

Affinity Capture-MS

Homo sapiens

IREB2

3658

Affinity Capture-MS

Homo sapiens

DNAJC2

27000

Affinity Capture-MS

Homo sapiens

RIC8B

55188

Affinity Capture-MS

Homo sapiens

POP7

Affinity Capture-MS

Homo sapiens

ATP12A

479

Affinity Capture-MS

Homo sapiens

XPA

Affinity Capture-MS

Homo sapiens

SQSTM1

8878

Proximity Label-MS

Homo sapiens

UBB

7314

Affinity Capture-MS

Homo sapiens

DDX19B

11269

Affinity Capture-MS

Homo sapiens

MTMR4

Affinity Capture-MS

Homo sapiens

DCTN1

1639

Proximity Label-MS

Homo sapiens

OFD1

8481

Affinity Capture-MS

Homo sapiens

CHCHD4

Affinity Capture-MS

Homo sapiens

RPP25

Affinity Capture-MS

Homo sapiens

B3GNT2

10678

Affinity Capture-MS

Homo sapiens

PPP2R1A

5518

Affinity Capture-MS

Homo sapiens

BBS1

582

Affinity Capture-MS

Homo sapiens

RABGGTA

5875

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

BICD1

636

Proximity Label-MS

Homo sapiens

CBX5

23468

Affinity Capture-MS

Homo sapiens

CLTA

1211

Proximity Label-MS

Homo sapiens

PEX6

Affinity Capture-MS

Homo sapiens

PPP2CA

5515

Affinity Capture-MS

Homo sapiens

ENO2

2026

Two-hybrid

Homo sapiens

PRPF19

27339

Cross-Linking-MS (XL-MS)

Homo sapiens

USP4

7375

Affinity Capture-MS

Homo sapiens

RAB11FIP5

26056

Co-fractionation

Homo sapiens

PPP2R2A

5520

Affinity Capture-MS

Homo sapiens

PPP2R2D

55844

Affinity Capture-MS		Homo sapiens
Proximity Label-MS		Homo sapiens

PROSC

11212

Co-fractionation

Homo sapiens

PPP2CB

5516

Affinity Capture-MS

Homo sapiens

SCCPDH

51097

Affinity Capture-MS

Homo sapiens

PPME1

51400

Affinity Capture-MS

Homo sapiens

DNAJC22

Affinity Capture-MS

Homo sapiens

RAP2A

5911

Two-hybrid

Homo sapiens

View the network

Pathways in which ENSA is involved

Pathway	Evidence	Source
Cell Cycle	TAS	Reactome
Cell Cycle	IEA	Reactome
Cell Cycle, Mitotic	TAS	Reactome
Cell Cycle, Mitotic	IEA	Reactome
M Phase	TAS	Reactome
M Phase	IEA	Reactome
MASTL Facilitates Mitotic Progression	TAS	Reactome
MASTL Facilitates Mitotic Progression	IEA	Reactome
Mitotic Prophase	TAS	Reactome
Mitotic Prophase	IEA	Reactome

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