Gene description for SAMHD1
Gene name SAM domain and HD domain 1
Gene symbol SAMHD1
Other names/aliases CHBL2
DCIP
HDDC1
MOP-5
SBBI88
Species Homo sapiens
 Database cross references - SAMHD1
ExoCarta ExoCarta_25939
Vesiclepedia VP_25939
Entrez Gene 25939
HGNC 15925
MIM 606754
UniProt Q9Y3Z3  
 SAMHD1 identified in sEVs derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Endothelial cells 26027894    
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for SAMHD1
Molecular Function
    nucleic acid binding GO:0003676 IDA
    single-stranded DNA binding GO:0003697 IDA
    RNA binding GO:0003723 IDA
    RNA nuclease activity GO:0004540 IDA
    RNA nuclease activity GO:0004540 IDA
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IEA
    zinc ion binding GO:0008270 IDA
    dGTPase activity GO:0008832 IBA
    dGTPase activity GO:0008832 IDA
    dGTP binding GO:0032567 IDA
    identical protein binding GO:0042802 IPI
    deoxynucleoside triphosphate hydrolase activity GO:0106375 IDA
Biological Process
    double-strand break repair via homologous recombination GO:0000724 IDA
    dGTP catabolic process GO:0006203 IBA
    dGTP catabolic process GO:0006203 IDA
    immune response GO:0006955 NAS
    DNA damage response GO:0006974 IDA
    deoxyribonucleotide catabolic process GO:0009264 IDA
    somatic hypermutation of immunoglobulin genes GO:0016446 ISS
    innate immune response GO:0045087 IEA
    regulation of innate immune response GO:0045088 IBA
    regulation of innate immune response GO:0045088 IDA
    regulation of innate immune response GO:0045088 IMP
    dATP catabolic process GO:0046061 IDA
    protein homotetramerization GO:0051289 IDA
    defense response to virus GO:0051607 IBA
    defense response to virus GO:0051607 IDA
    defense response to virus GO:0051607 IMP
    negative regulation of type I interferon-mediated signaling pathway GO:0060339 IDA
    DNA strand resection involved in replication fork processing GO:0110025 IDA
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    plasma membrane GO:0005886 IDA
    site of double-strand break GO:0035861 IDA
    tetraspanin-enriched microdomain GO:0097197 IDA
 Experiment description of studies that identified SAMHD1 in sEVs
1
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 226
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD9|FLOT1
Enriched markers
✔
LMNA|H2AFX|ATP5A1|TOMM20
Negative markers
✘ Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description "Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting"
Authors "Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar"
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
7
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SAMHD1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 RSBN1L  
Affinity Capture-MS Homo sapiens
3 SPRTN  
Affinity Capture-MS Homo sapiens
4 GART 2618
Co-fractionation Homo sapiens
5 SOGA1 140710
Affinity Capture-MS Homo sapiens
6 MSH6 2956
Co-fractionation Homo sapiens
7 ATG5 9474
Proximity Label-MS Homo sapiens
8 AKAP12 9590
Affinity Capture-MS Homo sapiens
9 RACGAP1 29127
Affinity Capture-MS Homo sapiens
10 VPRBP 9730
Biochemical Activity Homo sapiens
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
11 FBXO25  
Biochemical Activity Homo sapiens
12 HSF5  
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
13 KANSL3  
Affinity Capture-MS Homo sapiens
14 SMARCD2 6603
Affinity Capture-MS Homo sapiens
15 APP 351
Reconstituted Complex Homo sapiens
16 GSK3A 2931
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 KIAA1429 25962
Affinity Capture-MS Homo sapiens
18 GPATCH4 54865
Affinity Capture-MS Homo sapiens
19 FAM13B  
Two-hybrid Homo sapiens
20 CASP3 836
Co-fractionation Homo sapiens
21 COPS6 10980
Affinity Capture-MS Homo sapiens
22 FAM174A 345757
Affinity Capture-MS Homo sapiens
23 OSBP 5007
Co-fractionation Homo sapiens
24 USP18  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
25 FBXW7  
Affinity Capture-MS Homo sapiens
26 RPL24 6152
Co-fractionation Homo sapiens
27 Naa10  
Affinity Capture-MS Mus musculus
28 HSPH1 10808
Co-fractionation Homo sapiens
29 NARS 4677
Co-fractionation Homo sapiens
30 XPO7 23039
Co-fractionation Homo sapiens
31 FAF1 11124
Co-fractionation Homo sapiens
32 TRMT1 55621
Co-fractionation Homo sapiens
33 SEC23A 10484
Co-fractionation Homo sapiens
34 EEF1A1 1915
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Co-localization Homo sapiens
35 GSK3B 2932
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 SAMHD1 25939
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
37 CHMP4C 92421
Affinity Capture-MS Homo sapiens
38 ZMAT3  
Affinity Capture-MS Homo sapiens
39 RBX1 9978
Affinity Capture-Western Homo sapiens
40 USP11 8237
Affinity Capture-MS Homo sapiens
41 CTDP1 9150
Affinity Capture-MS Homo sapiens
42 PARK2  
Affinity Capture-MS Homo sapiens
43 COPE 11316
Affinity Capture-MS Homo sapiens
44 SUPT5H 6829
Co-fractionation Homo sapiens
45 PLOD2 5352
Co-fractionation Homo sapiens
46 BARD1 580
Affinity Capture-MS Homo sapiens
47 NTRK1 4914
Affinity Capture-MS Homo sapiens
48 U2AF2 11338
Affinity Capture-MS Homo sapiens
49 XRCC5 7520
Co-fractionation Homo sapiens
50 KPNB1 3837
Affinity Capture-Western Homo sapiens
51 ACTB 60
Reconstituted Complex Homo sapiens
52 GANAB 23193
Co-fractionation Homo sapiens
53 HECTD1 25831
Affinity Capture-MS Homo sapiens
54 CUL2 8453
Affinity Capture-MS Homo sapiens
55 SKP2  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
56 ATP6V1E1 529
Co-fractionation Homo sapiens
57 TIRAP  
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
58 VCP 7415
Affinity Capture-MS Homo sapiens
59 CCNA2 890
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
60 CUL3 8452
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 AMBRA1  
Affinity Capture-MS Homo sapiens
62 DUSP14 11072
Affinity Capture-MS Homo sapiens
63 LCOR 84458
Affinity Capture-MS Homo sapiens
64 PRSS50  
Affinity Capture-MS Homo sapiens
65 SF1 7536
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
66 EGFR 1956
Affinity Capture-MS Homo sapiens
67 MAP3K7  
Affinity Capture-Western Homo sapiens
68 RIPK4  
Affinity Capture-MS Homo sapiens
69 RANBP3 8498
Co-fractionation Homo sapiens
70 ADAM7  
Affinity Capture-MS Homo sapiens
71 CDK2 1017
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
72 PPP2R1A 5518
Affinity Capture-MS Homo sapiens
73 RPA3 6119
Proximity Label-MS Homo sapiens
74 PPP2R5E 5529
Affinity Capture-MS Homo sapiens
75 SELENBP1 8991
Affinity Capture-MS Homo sapiens
76 GPR45  
Affinity Capture-MS Homo sapiens
77 DDB1 1642
Affinity Capture-Western Homo sapiens
78 CCNA1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
79 RNF2  
Affinity Capture-MS Homo sapiens
80 AIP 9049
Affinity Capture-MS Homo sapiens
81 CHUK 1147
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
82 NAA10 8260
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
83 HIF1A 3091
Affinity Capture-MS Homo sapiens
84 PRMT3 10196
Co-fractionation Homo sapiens
85 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
86 NAAA  
Affinity Capture-MS Homo sapiens
87 PARP1 142
Affinity Capture-MS Homo sapiens
88 JUP 3728
Co-fractionation Homo sapiens
89 STX3 6809
Affinity Capture-MS Homo sapiens
90 TUBG1 7283
Co-fractionation Homo sapiens
91 POLR3H  
Affinity Capture-MS Homo sapiens
92 CUL5 8065
Affinity Capture-MS Homo sapiens
93 CDK1 983
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
94 TMEM25  
Affinity Capture-MS Homo sapiens
95 EIF4B 1975
Co-fractionation Homo sapiens
96 DSG4 147409
Affinity Capture-MS Homo sapiens
97 XRCC6 2547
Co-fractionation Homo sapiens
98 C9orf72  
Affinity Capture-MS Homo sapiens
99 KBTBD4  
Affinity Capture-MS Homo sapiens
100 MTCL1 23255
Affinity Capture-MS Homo sapiens
101 IKBKB 3551
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
102 CUL4A 8451
Affinity Capture-Western Homo sapiens
103 IKBKG 8517
Affinity Capture-Western Homo sapiens
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