Gene description for AXIN2
Gene name axin 2
Gene symbol AXIN2
Other names/aliases AXIL
ODCRCS
Species Homo sapiens
 Database cross references - AXIN2
ExoCarta ExoCarta_8313
Entrez Gene 8313
HGNC 904
MIM 604025
UniProt Q9Y2T1  
 AXIN2 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Hepatocytes 26054723    
 Gene ontology annotations for AXIN2
Molecular Function
    GTPase activator activity GO:0005096 IBA
    I-SMAD binding GO:0070411 IEA
    armadillo repeat domain binding GO:0070016 IBA
    beta-catenin binding GO:0008013 NAS
    protein binding GO:0005515 IPI
    protein kinase binding GO:0019901 IBA
    ubiquitin protein ligase binding GO:0031625 IPI
    enzyme binding GO:0019899 IPI
Biological Process
    regulation of transcription, DNA-templated GO:0006355 IBA
    negative regulation of cell proliferation GO:0008285 IMP
    regulation of mismatch repair GO:0032423 IMP
    positive regulation of epithelial to mesenchymal transition GO:0010718 IMP
    negative regulation of canonical Wnt signaling pathway GO:0090090 IMP
    positive regulation of cell death GO:0010942 IMP
    cellular protein localization GO:0034613 IDA
    intramembranous ossification GO:0001957 IBA
    positive regulation of protein phosphorylation GO:0001934 IMP
    cell death GO:0008219 IBA
    regulation of centromeric sister chromatid cohesion GO:0070602 IMP
    Wnt signaling pathway involved in somitogenesis GO:0090244 IBA
    cellular response to organic cyclic compound GO:0071407 IBA
    cell proliferation GO:0008283 IEA
    negative regulation of osteoblast differentiation GO:0045668 IBA
    cell differentiation GO:0030154 IBA
    dorsal/ventral axis specification GO:0009950 IBA
    odontogenesis GO:0042476 IMP
    negative regulation of catenin import into nucleus GO:0035414 IMP
    chondrocyte differentiation involved in endochondral bone morphogenesis GO:0003413 IEA
    termination of G-protein coupled receptor signaling pathway GO:0038032 IEA
    mRNA stabilization GO:0048255 IMP
    secondary heart field specification GO:0003139 IEA
    maintenance of DNA repeat elements GO:0043570 IMP
    regulation of chondrocyte development GO:0061181 IEA
    positive regulation of canonical Wnt signaling pathway GO:0090263 TAS
    bone mineralization GO:0030282 IEA
    positive regulation of GTPase activity GO:0043547 IBA
Subcellular Localization
    cytosol GO:0005829 TAS
    postsynaptic density GO:0014069 IBA
    cell cortex GO:0005938 IBA
    cytoplasmic microtubule GO:0005881 IBA
    centrosome GO:0005813 IDA
    microtubule cytoskeleton GO:0015630 IDA
    plasma membrane GO:0005886 IBA
    Golgi apparatus GO:0005794 IDA
    cytoplasm GO:0005737 IDA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    beta-catenin destruction complex GO:0030877 NAS
    cytoplasmic membrane-bounded vesicle GO:0016023 IBA
 Experiment description of studies that identified AXIN2 in exosomes
1
Experiment ID 234
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-C3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 237
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for AXIN2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 APC  
Invitro Homo sapiens
Invivo Homo sapiens
Two-hybrid Homo sapiens
2 ANKRD6  
Invivo Homo sapiens
Two-hybrid Homo sapiens
3 CTNNB1 1499
Invitro Homo sapiens
Invivo Homo sapiens
Two-hybrid Homo sapiens
4 APC2  
Two-hybrid Homo sapiens
5 GSK3B 2932
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
View the network image/svg+xml



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