Gene description for GIT1
Gene name G protein-coupled receptor kinase interacting ArfGAP 1
Gene symbol GIT1
Other names/aliases -
Species Homo sapiens
 Database cross references - GIT1
ExoCarta ExoCarta_28964
Vesiclepedia VP_28964
Entrez Gene 28964
HGNC 4272
MIM 608434
UniProt Q9Y2X7  
 GIT1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for GIT1
Molecular Function
    GTPase activator activity GO:0005096 IBA
    protein binding GO:0005515 IPI
    small GTPase binding GO:0031267 IBA
    gamma-tubulin binding GO:0043015 IDA
    protein-containing complex binding GO:0044877 IEA
    metal ion binding GO:0046872 IEA
Biological Process
    intramembranous ossification GO:0001957 ISS
    brain development GO:0007420 IBA
    locomotory behavior GO:0007626 IEA
    regulation of G protein-coupled receptor signaling pathway GO:0008277 IBA
    regulation of ARF protein signal transduction GO:0032012 IBA
    regulation of cytokinesis GO:0032465 IDA
    negative regulation of interleukin-1 beta production GO:0032691 ISS
    synaptic vesicle recycling GO:0036465 IBA
    cell redox homeostasis GO:0045454 ISS
    negative regulation of glycolytic process GO:0045820 ISS
    ephrin receptor signaling pathway GO:0048013 IEA
    neuron development GO:0048666 IEA
    motor learning GO:0061743 IEA
    cellular response to lipopolysaccharide GO:0071222 ISS
    positive regulation of microtubule nucleation GO:0090063 IMP
    presynaptic modulation of chemical synaptic transmission GO:0099171 IEA
    negative regulation of inflammatory response to wounding GO:0106015 ISS
    regulation of synaptic vesicle exocytosis GO:2000300 IEA
Subcellular Localization
    mitochondrion GO:0005739 IDA
    centrosome GO:0005813 IDA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    focal adhesion GO:0005925 HDA
    focal adhesion GO:0005925 IDA
    postsynaptic density GO:0014069 IEA
    membrane GO:0016020 HDA
    lamellipodium GO:0030027 IEA
    neuron projection GO:0043005 IBA
    calyx of Held GO:0044305 IEA
    synapse GO:0045202 IBA
    mitotic spindle pole GO:0097431 IDA
    postsynapse GO:0098794 IBA
    postsynapse GO:0098794 ISS
 Experiment description of studies that identified GIT1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for GIT1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PTPRF 5792
Affinity Capture-Western Homo sapiens
2 SKP1 6500
Affinity Capture-MS Homo sapiens
3 SENP3 26168
Affinity Capture-MS Homo sapiens
4 HOOK1  
Proximity Label-MS Homo sapiens
5 ZNHIT1  
Affinity Capture-MS Homo sapiens
6 DDX24 57062
Two-hybrid Homo sapiens
7 KPNA1 3836
Affinity Capture-MS Homo sapiens
8 RGS2  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
9 CCDC113  
Two-hybrid Homo sapiens
10 GIT2 9815
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Co-localization Homo sapiens
11 PAK2 5062
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 TAB1 10454
Two-hybrid Homo sapiens
13 ADRBK1 156
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
14 RABGGTB 5876
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 SNX6 58533
Affinity Capture-Western Homo sapiens
16 PMF1  
Two-hybrid Homo sapiens
17 APEX1 328
Affinity Capture-RNA Homo sapiens
18 CENPU  
Two-hybrid Homo sapiens
19 TMEM154  
Affinity Capture-MS Homo sapiens
20 TAX1BP3 30851
Affinity Capture-MS Homo sapiens
21 MAFF 23764
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 PPFIA3 8541
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
23 EXOC1 55763
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 D2HGDH 728294
Affinity Capture-MS Homo sapiens
25 PTPRZ1  
Affinity Capture-Western Homo sapiens
26 FBXW7  
Affinity Capture-MS Homo sapiens
27 SCRIB 23513
Affinity Capture-MS Homo sapiens
28 FBXO28  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 YWHAG 7532
Affinity Capture-MS Homo sapiens
30 PLCG1 5335
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
31 ADRBK2  
Affinity Capture-Western Homo sapiens
32 CENPQ  
Affinity Capture-MS Homo sapiens
33 YWHAH 7533
Affinity Capture-MS Homo sapiens
34 HAP1  
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
35 SNX27 81609
Affinity Capture-MS Homo sapiens
36 CHD3 1107
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
37 NCK1 4690
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 MED4 29079
Affinity Capture-MS Homo sapiens
39 PAK1 5058
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
40 PAK3  
Affinity Capture-MS Homo sapiens
41 C1orf106  
Affinity Capture-MS Homo sapiens
42 PPFIA2 8499
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
43 CAMK2A 815
Affinity Capture-Western Homo sapiens
44 FAM96A  
Affinity Capture-MS Homo sapiens
45 RNF113B  
Affinity Capture-MS Homo sapiens
46 NCKIPSD 51517
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 SDCCAG3  
Two-hybrid Homo sapiens
48 LRIF1  
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
49 HTT 3064
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
50 SRRT 51593
Two-hybrid Homo sapiens
51 GIT1 28964
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
52 MAN2A2 4122
Two-hybrid Homo sapiens
53 BARD1 580
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
54 NTRK1 4914
Affinity Capture-MS Homo sapiens
55 SYNC  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 PTK2B 2185
Affinity Capture-Western Homo sapiens
57 PCLO 27445
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
58 ARHGEF6 9459
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
59 SLC33A1 9197
Affinity Capture-MS Homo sapiens
60 UBXN6 80700
Affinity Capture-MS Homo sapiens
61 POTEB3 102724631
Affinity Capture-MS Homo sapiens
62 NCKAP5L  
Affinity Capture-MS Homo sapiens
63 ICAM1 3383
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 TMOD4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 EIF6 3692
Two-hybrid Homo sapiens
66 HMOX2 3163
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
67 ERC2 26059
Affinity Capture-Western Homo sapiens
68 WDR33 55339
Two-hybrid Homo sapiens
69 SLC9A3R2 9351
Affinity Capture-MS Homo sapiens
70 TFPT  
Affinity Capture-MS Homo sapiens
71 PTK2 5747
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
72 METTL21B  
Affinity Capture-MS Homo sapiens
73 PDPK1 5170
Two-hybrid Homo sapiens
74 PKN3 29941
Affinity Capture-MS Homo sapiens
75 LAMTOR5 10542
Two-hybrid Homo sapiens
76 TXNDC9 10190
Two-hybrid Homo sapiens
77 CDC42 998
Affinity Capture-MS Homo sapiens
78 ARF6 382
Affinity Capture-MS Homo sapiens
79 CAMK4 814
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
80 GRIP2 80852
Affinity Capture-Western Homo sapiens
81 CSNK2A2 1459
Affinity Capture-MS Homo sapiens
82 PSMC5 5705
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
83 CROCC 9696
Affinity Capture-MS Homo sapiens
84 PPFIA1 8500
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
85 ARHGEF7 8874
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
86 C8orf33 65265
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
87 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
88 SIRT6  
Affinity Capture-MS Homo sapiens
89 GRB2 2885
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
90 COPA 1314
Affinity Capture-MS Homo sapiens
91 EPHB2 2048
Affinity Capture-MS Homo sapiens
92 SNRPA 6626
Co-fractionation Homo sapiens
93 PFDN1 5201
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
94 NINL  
Proximity Label-MS Homo sapiens
95 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
96 PHYKPL  
Affinity Capture-MS Homo sapiens
97 LPXN 9404
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
98 NCK2 8440
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
99 KLHL1  
Affinity Capture-MS Homo sapiens
100 TERF1 7013
Two-hybrid Homo sapiens
101 NEK2  
Two-hybrid Homo sapiens
102 C20orf195  
Affinity Capture-MS Homo sapiens
103 PPFIA4  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
104 PXN 5829
Affinity Capture-Western Homo sapiens
Proximity Label-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
105 PGK1 5230
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
106 SLC9A3R1 9368
Affinity Capture-MS Homo sapiens
107 ARF1 375
Affinity Capture-MS Homo sapiens
108 TRIB3  
Two-hybrid Homo sapiens
109 EPHA2 1969
Affinity Capture-MS Homo sapiens
110 RAC1 5879
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
111 RPA3 6119
Proximity Label-MS Homo sapiens
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