Gene description for GNPAT
Gene name glyceronephosphate O-acyltransferase
Gene symbol GNPAT
Other names/aliases DAP-AT
DAPAT
DHAPAT
Species Homo sapiens
 Database cross references - GNPAT
ExoCarta ExoCarta_8443
Vesiclepedia VP_8443
Entrez Gene 8443
HGNC 4416
MIM 602744
UniProt O15228  
 GNPAT identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for GNPAT
Molecular Function
    glycerone-phosphate O-acyltransferase activity GO:0016287 IBA
    glycerone-phosphate O-acyltransferase activity GO:0016287 IDA
    glycerone-phosphate O-acyltransferase activity GO:0016287 TAS
Biological Process
    phosphatidic acid biosynthetic process GO:0006654 TAS
    synapse assembly GO:0007416 IEA
    response to nutrient GO:0007584 IEA
    ether lipid biosynthetic process GO:0008611 IBA
    ether lipid biosynthetic process GO:0008611 IDA
    ether lipid biosynthetic process GO:0008611 TAS
    response to xenobiotic stimulus GO:0009410 IEA
    cerebellum morphogenesis GO:0021587 IEA
    paranodal junction assembly GO:0030913 IEA
    response to starvation GO:0042594 IEA
    membrane organization GO:0061024 IEA
    response to fatty acid GO:0070542 IEA
Subcellular Localization
    peroxisome GO:0005777 IDA
    peroxisome GO:0005777 NAS
    peroxisomal membrane GO:0005778 HDA
    peroxisomal membrane GO:0005778 IBA
    peroxisomal membrane GO:0005778 TAS
    peroxisomal matrix GO:0005782 TAS
    cytosol GO:0005829 TAS
    membrane GO:0016020 HDA
 Experiment description of studies that identified GNPAT in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 834
MISEV standards
EM
Biophysical techniques
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 835
MISEV standards
EM
Biophysical techniques
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for GNPAT
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 C6orf222  
Affinity Capture-MS Homo sapiens
2 GRPR  
Affinity Capture-MS Homo sapiens
3 CALML3 810
Affinity Capture-MS Homo sapiens
4 CLPP 8192
Proximity Label-MS Homo sapiens
5 C1QTNF2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 FAM131B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 LYPD3 27076
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 APOA2 336
Affinity Capture-MS Homo sapiens
9 VDR  
Two-hybrid Homo sapiens
10 CYB5B 80777
Affinity Capture-MS Homo sapiens
11 HEBP1 50865
Co-fractionation Homo sapiens
12 COIL  
Proximity Label-MS Homo sapiens
13 IFT20 90410
Affinity Capture-MS Homo sapiens
14 PMF1-BGLAP  
Affinity Capture-MS Homo sapiens
15 XPO1 7514
Affinity Capture-MS Homo sapiens
16 SLC39A4 55630
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 HCST  
Affinity Capture-MS Homo sapiens
18 BSG 682
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 ENPP6 133121
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 NPTN 27020
Affinity Capture-MS Homo sapiens
21 GPR3  
Two-hybrid Homo sapiens
22 TMEM206 55248
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 S100A7L2  
Affinity Capture-MS Homo sapiens
24 C9orf78 51759
Affinity Capture-MS Homo sapiens
25 ARL2BP 23568
Affinity Capture-MS Homo sapiens
26 HSPB1 3315
Two-hybrid Homo sapiens
27 GPR182  
Affinity Capture-MS Homo sapiens
28 DCAF11  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 RB1CC1 9821
Affinity Capture-MS Homo sapiens
30 GAL  
Affinity Capture-MS Homo sapiens
31 FAM63B 54629
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 GHRL  
Affinity Capture-MS Homo sapiens
33 S100A9 6280
Affinity Capture-MS Homo sapiens
34 ATXN7L1 222255
Affinity Capture-MS Homo sapiens
35 FFAR1  
Affinity Capture-MS Homo sapiens
36 DLL1  
Affinity Capture-MS Homo sapiens
37 PMF1  
Affinity Capture-MS Homo sapiens
38 IL1R2  
Affinity Capture-MS Homo sapiens
39 FANCE  
Affinity Capture-MS Homo sapiens
40 HDAC10  
Affinity Capture-MS Homo sapiens
41 SAAL1 113174
Affinity Capture-MS Homo sapiens
42 PHYH 5264
Affinity Capture-MS Homo sapiens
43 RPA3 6119
Proximity Label-MS Homo sapiens
44 BARD1 580
Affinity Capture-MS Homo sapiens
45 C16orf72 29035
Affinity Capture-MS Homo sapiens
46 NTRK1 4914
Affinity Capture-MS Homo sapiens
47 SCGB2A2  
Affinity Capture-MS Homo sapiens
48 GPC3 2719
Affinity Capture-MS Homo sapiens
49 PEX14 5195
Co-purification Homo sapiens
50 TGFA  
Affinity Capture-MS Homo sapiens
51 HUWE1 10075
Affinity Capture-MS Homo sapiens
52 PEX5 5830
Protein-peptide Homo sapiens
Affinity Capture-MS Homo sapiens
53 PVR 5817
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 SNRPC 6631
Affinity Capture-MS Homo sapiens
55 C3AR1  
Affinity Capture-MS Homo sapiens
56 C11orf74  
Affinity Capture-MS Homo sapiens
57 C16orf71  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 PLD6  
Affinity Capture-MS Homo sapiens
59 AGPS 8540
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Proximity Label-MS Homo sapiens
60 LYPD4  
Affinity Capture-MS Homo sapiens
61 TSPAN10 83882
Affinity Capture-MS Homo sapiens
62 TTC25  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 GIP  
Affinity Capture-MS Homo sapiens
64 HYPM  
Affinity Capture-MS Homo sapiens
65 SLC7A1 6541
Affinity Capture-MS Homo sapiens
66 PROSER2 254427
Affinity Capture-MS Homo sapiens
67 ZNF544  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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