Gene description for SPAG5
Gene name sperm associated antigen 5
Gene symbol SPAG5
Other names/aliases DEEPEST
MAP126
hMAP126
Species Homo sapiens
 Database cross references - SPAG5
ExoCarta ExoCarta_10615
Vesiclepedia VP_10615
Entrez Gene 10615
HGNC 13452
MIM 615562
UniProt Q96R06  
 SPAG5 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Urine 21595033    
Urine 21595033    
Urine 21595033    
 Gene ontology annotations for SPAG5
Molecular Function
    protein binding GO:0005515 IPI
    microtubule binding GO:0008017 IDA
Biological Process
    mitotic sister chromatid segregation GO:0000070 IMP
    spindle organization GO:0007051 IMP
    chromosome segregation GO:0007059 IMP
    positive regulation of intracellular transport GO:0032388 IMP
    establishment of spindle orientation GO:0051294 IEA
    cell division GO:0051301 IEA
    regulation of attachment of spindle microtubules to kinetochore GO:0051988 IMP
    protein localization to centrosome GO:0071539 IMP
    regulation of metaphase plate congression GO:0090235 IMP
    positive regulation of spindle assembly GO:1905832 IMP
Subcellular Localization
    kinetochore GO:0000776 IDA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IDA
    nuclear body GO:0016604 IDA
    midbody GO:0030496 IEA
    centriolar satellite GO:0034451 IMP
    ciliary rootlet GO:0035253 IEA
    microtubule plus-end GO:0035371 IDA
    ciliary basal body GO:0036064 IEA
    mitotic spindle GO:0072686 IDA
    mitotic spindle pole GO:0097431 IDA
 Experiment description of studies that identified SPAG5 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 193
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|CD9
Enriched markers
✔
PHB
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 194
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of basement membrane nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 195
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of early IgA nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SPAG5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 KRT37 8688
Affinity Capture-MS Homo sapiens
2 LGALS14  
Two-hybrid Homo sapiens
3 SLC9C1 285335
Affinity Capture-MS Homo sapiens
4 SYCE1  
Affinity Capture-MS Homo sapiens
5 FMR1 2332
Two-hybrid Homo sapiens
6 CEP170 9859
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 SMARCE1 6605
Two-hybrid Homo sapiens
8 ENKD1  
Two-hybrid Homo sapiens
9 RBM41  
Two-hybrid Homo sapiens
10 BFSP2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 RIC8A 60626
Affinity Capture-MS Homo sapiens
12 MID1 4281
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
13 ATN1  
Two-hybrid Homo sapiens
14 EIF4E2  
Two-hybrid Homo sapiens
15 ZMAT2  
Two-hybrid Homo sapiens
16 RHPN1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 MRFAP1L1  
Affinity Capture-MS Homo sapiens
18 EMILIN1 11117
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 SNAPIN 23557
Two-hybrid Homo sapiens
20 SYCE3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 RINT1 60561
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 Cep55 74107
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
23 OSBP 5007
Affinity Capture-MS Homo sapiens
24 FBXO28  
Two-hybrid Homo sapiens
25 ZGPAT  
Two-hybrid Homo sapiens
26 Ankrd26  
Affinity Capture-MS Mus musculus
27 GOLGA8EP  
Two-hybrid Homo sapiens
28 ORC6  
Two-hybrid Homo sapiens
29 Knstrn  
Affinity Capture-MS Mus musculus
30 DYNLL1 8655
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 TUBA3C 7278
Affinity Capture-MS Homo sapiens
32 Mcm3 17215
Affinity Capture-MS Mus musculus
33 FSD2 123722
Affinity Capture-MS Homo sapiens
34 KNSTRN  
Affinity Capture-MS Homo sapiens
35 ARNT2  
Two-hybrid Homo sapiens
36 AMOT  
Proximity Label-MS Homo sapiens
37 Spag5  
Affinity Capture-MS Mus musculus
38 KIF14 9928
Affinity Capture-MS Homo sapiens
39 IFT20 90410
Affinity Capture-MS Homo sapiens
40 CCDC114  
Affinity Capture-MS Homo sapiens
41 NINL  
Proximity Label-MS Homo sapiens
42 IQCE 23288
Two-hybrid Homo sapiens
43 MED1 5469
Affinity Capture-MS Homo sapiens
44 PFN1 5216
Proximity Label-MS Homo sapiens
45 KRT8 3856
Proximity Label-MS Homo sapiens
46 ZNF385C  
Two-hybrid Homo sapiens
47 SNAP47 116841
Two-hybrid Homo sapiens
48 RABEP2 79874
Affinity Capture-MS Homo sapiens
49 SRGAP3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 GOLGA8F  
Two-hybrid Homo sapiens
51 Cep44  
Affinity Capture-MS Mus musculus
52 LINGO1 84894
Two-hybrid Homo sapiens
53 CHCHD3 54927
Two-hybrid Homo sapiens
54 CCDC33 80125
Two-hybrid Homo sapiens
55 MAPRE1 22919
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 PSMD14 10213
Affinity Capture-MS Homo sapiens
57 ANKRD30A 91074
Affinity Capture-MS Homo sapiens
58 DTNBP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 MID2 11043
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
60 BARD1 580
Two-hybrid Homo sapiens
61 PLK1 5347
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 DCTN1 1639
Proximity Label-MS Homo sapiens
63 PEX14 5195
Proximity Label-MS Homo sapiens
64 CCDC22 28952
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 DCLRE1B  
Reconstituted Complex Homo sapiens
66 DYNLL2 140735
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 ZFC3H1 196441
Two-hybrid Homo sapiens
68 COPS7B 64708
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 AGPS 8540
Proximity Label-MS Homo sapiens
70 NEURL4  
Affinity Capture-MS Homo sapiens
71 ANLN 54443
Affinity Capture-MS Homo sapiens
72 PHLPP1  
Proximity Label-MS Homo sapiens
73 DISC1 27185
Affinity Capture-MS Homo sapiens
74 WHAMMP3  
Affinity Capture-MS Homo sapiens
75 MAP4 4134
Affinity Capture-MS Homo sapiens
76 IFT57 55081
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
77 CUL3 8452
Affinity Capture-MS Homo sapiens
78 YWHAZ 7534
Affinity Capture-MS Homo sapiens
79 TUBB4B 10383
Affinity Capture-MS Homo sapiens
80 HOOK3 84376
Proximity Label-MS Homo sapiens
81 DUSP16  
Affinity Capture-MS Homo sapiens
82 TTC23  
Two-hybrid Homo sapiens
83 SASS6 163786
Proximity Label-MS Homo sapiens
84 Cep152  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
85 KRT27 342574
Affinity Capture-MS Homo sapiens
86 KIAA1467 57613
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
87 AURKB 9212
Affinity Capture-Western Homo sapiens
88 MAPRE3  
Proximity Label-MS Homo sapiens
89 COG6 57511
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
90 HECW2  
Affinity Capture-MS Homo sapiens
91 NIN 51199
Proximity Label-MS Homo sapiens
92 CCDC144B  
Affinity Capture-MS Homo sapiens
93 NDC80 10403
Proximity Label-MS Homo sapiens
94 DCX  
Two-hybrid Homo sapiens
95 Dynll1 56455
Affinity Capture-MS Mus musculus
96 PCM1 5108
Proximity Label-MS Homo sapiens
97 CDC20B  
Two-hybrid Homo sapiens
98 Mad2l1 56150
Affinity Capture-MS Mus musculus
99 ZC2HC1C  
Two-hybrid Homo sapiens
100 KRT19 3880
Proximity Label-MS Homo sapiens
101 KRT222 125113
Affinity Capture-MS Homo sapiens
102 Plk1  
Affinity Capture-MS Mus musculus
103 ACBD5 91452
Proximity Label-MS Homo sapiens
104 KRT18 3875
Proximity Label-MS Homo sapiens
105 MYCBP 26292
Affinity Capture-MS Homo sapiens
106 CEP170P1  
Affinity Capture-MS Homo sapiens
107 TSHZ3  
Two-hybrid Homo sapiens
108 HAUS1  
Two-hybrid Homo sapiens
109 CCHCR1  
Two-hybrid Homo sapiens
110 POLR2L 5441
Two-hybrid Homo sapiens
111 BFSP1  
Affinity Capture-MS Homo sapiens
112 TSKS  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
113 NLGN3  
Two-hybrid Homo sapiens
114 CCDC89  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
115 SEPT10 151011
Proximity Label-MS Homo sapiens
116 DCTN2 10540
Proximity Label-MS Homo sapiens
117 CLUH 23277
Affinity Capture-MS Homo sapiens
118 KANSL1  
Two-hybrid Homo sapiens
119 TSPYL1 7259
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
120 CDK1 983
Biochemical Activity Homo sapiens
121 ANAPC2 29882
Proximity Label-MS Homo sapiens
122 MED21  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
123 ABI2 10152
Affinity Capture-MS Homo sapiens
124 Mapre1 13589
Affinity Capture-MS Mus musculus
125 KRT38 8687
Affinity Capture-MS Homo sapiens
126 AMOTL2 51421
Two-hybrid Homo sapiens
127 C1orf109  
Two-hybrid Homo sapiens
128 KXD1 79036
Affinity Capture-MS Homo sapiens
129 KRT23  
Affinity Capture-MS Homo sapiens
130 SH2D4A 63898
Two-hybrid Homo sapiens
131 CCDC96  
Affinity Capture-MS Homo sapiens
132 CCDC67  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
133 ZFYVE26 23503
Two-hybrid Homo sapiens
134 RPA3 6119
Proximity Label-MS Homo sapiens
135 LURAP1  
Affinity Capture-MS Homo sapiens
136 CEBPD  
Affinity Capture-MS Homo sapiens
137 C17orf59 54785
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
138 EP300 2033
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which SPAG5 is involved
No pathways found





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