Gene description for MAGED1
Gene name melanoma antigen family D1
Gene symbol MAGED1
Other names/aliases DLXIN-1
NRAGE
Species Homo sapiens
 Database cross references - MAGED1
ExoCarta ExoCarta_9500
Vesiclepedia VP_9500
Entrez Gene 9500
HGNC 6813
MIM 300224
UniProt Q9Y5V3  
 MAGED1 identified in sEVs derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
 Gene ontology annotations for MAGED1
Molecular Function
    transcription coactivator activity GO:0003713 IEA
    protein binding GO:0005515 IPI
    identical protein binding GO:0042802 IPI
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IBA
    regulation of DNA-templated transcription GO:0006355 TAS
    circadian regulation of gene expression GO:0032922 ISS
    protein localization to nucleus GO:0034504 IEA
    regulation of circadian rhythm GO:0042752 IEA
    regulation of apoptotic process GO:0042981 TAS
    positive regulation of DNA-templated transcription GO:0045893 IEA
    negative regulation of epithelial cell proliferation GO:0050680 IDA
    positive regulation of branching involved in ureteric bud morphogenesis GO:0090190 IEA
    negative regulation of protein localization to nucleus GO:1900181 IEA
    positive regulation of apoptotic signaling pathway GO:2001235 IEA
Subcellular Localization
    chromatin GO:0000785 ISS
    nucleus GO:0005634 IBA
    nucleus GO:0005634 ISS
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IEA
    protein-containing complex GO:0032991 IDA
 Experiment description of studies that identified MAGED1 in sEVs
1
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for MAGED1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPH1 3187
Two-hybrid Homo sapiens
2 REC8  
Affinity Capture-MS Homo sapiens
3 COPG2 26958
Affinity Capture-MS Homo sapiens
4 SMC3 9126
Affinity Capture-MS Homo sapiens
5 SENP3 26168
Affinity Capture-MS Homo sapiens
6 PRR35  
Two-hybrid Homo sapiens
7 XIAP  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
8 ZIC1  
Two-hybrid Homo sapiens
9 AATF  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
10 FBXO7 25793
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
11 LENG8  
Two-hybrid Homo sapiens
12 YTHDF1 54915
Two-hybrid Homo sapiens
13 FMR1 2332
Affinity Capture-MS Homo sapiens
14 EBNA-LP  
Affinity Capture-MS
15 SPTLC1 10558
Affinity Capture-MS Homo sapiens
16 MDFI  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
17 DAB1 1600
Two-hybrid Homo sapiens
18 USP9X 8239
Affinity Capture-MS Homo sapiens
19 TAB1 10454
Affinity Capture-Western Homo sapiens
20 SLC25A1 6576
Affinity Capture-MS Homo sapiens
21 HSPB1 3315
Affinity Capture-MS Homo sapiens
22 KPNA2 3838
Two-hybrid Homo sapiens
23 RHOXF2  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
24 RPS6KB2  
Affinity Capture-MS Homo sapiens
25 ANKRD28 23243
Proximity Label-MS Homo sapiens
26 SOX2  
Affinity Capture-MS Homo sapiens
27 AKAP8L  
Affinity Capture-MS Homo sapiens
28 NUP160 23279
Affinity Capture-MS Homo sapiens
29 SMAP2 64744
Two-hybrid Homo sapiens
30 LRPPRC 10128
Affinity Capture-MS Homo sapiens
31 ZNF488  
Two-hybrid Homo sapiens
32 PJA1  
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
33 TRIM28 10155
Reconstituted Complex Homo sapiens
34 PINK1  
Affinity Capture-MS Homo sapiens
35 GLRA1  
Two-hybrid Homo sapiens
36 PCK1 5105
Affinity Capture-MS Homo sapiens
37 CA8  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
38 NUMBL 9253
Two-hybrid Homo sapiens
39 RNF8  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
40 TELO2 9894
Affinity Capture-MS Homo sapiens
41 TBX6  
Two-hybrid Homo sapiens
42 HGS 9146
Two-hybrid Homo sapiens
43 SNRPC 6631
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
44 KCTD21 283219
Affinity Capture-MS Homo sapiens
45 MAGED2 10916
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 RBPMS 11030
Two-hybrid Homo sapiens
47 CCDC33 80125
Two-hybrid Homo sapiens
48 EIF3E 3646
Affinity Capture-MS Homo sapiens
49 NOTO  
Two-hybrid Homo sapiens
50 DDX6 1656
Two-hybrid Homo sapiens
51 TIAL1 7073
Two-hybrid Homo sapiens
52 ARID5A  
Two-hybrid Homo sapiens
53 ATXN10 25814
Affinity Capture-MS Homo sapiens
54 PHF1  
Two-hybrid Homo sapiens
55 TIMM50 92609
Affinity Capture-MS Homo sapiens
56 NPAS1  
Affinity Capture-MS Homo sapiens
57 ARNT2  
Affinity Capture-Western Homo sapiens
FRET Homo sapiens
58 FAF2 23197
Affinity Capture-MS Homo sapiens
59 SOX5 6660
Two-hybrid Homo sapiens
60 FBXW5  
Affinity Capture-MS Homo sapiens
61 PRR20C  
Two-hybrid Homo sapiens
62 LARP4B 23185
Two-hybrid Homo sapiens
63 RNF185  
Affinity Capture-MS Homo sapiens
64 CPEB1  
Proximity Label-MS Homo sapiens
65 HEATR1 55127
Affinity Capture-MS Homo sapiens
66 TOLLIP 54472
Affinity Capture-MS Homo sapiens
67 NREP  
Affinity Capture-Western Homo sapiens
68 IRS4 8471
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
69 SULT1C4  
Affinity Capture-MS Homo sapiens
70 TSGA10IP  
Two-hybrid Homo sapiens
71 MIB1 57534
Proximity Label-MS Homo sapiens
72 MAPK3 5595
Two-hybrid Homo sapiens
73 RIN3  
Affinity Capture-MS Homo sapiens
74 TLX3  
Two-hybrid Homo sapiens
75 IRAK1  
Affinity Capture-MS Homo sapiens
76 CAPN7 23473
Two-hybrid Homo sapiens
77 RFX1  
Two-hybrid Homo sapiens
78 RBM23  
Two-hybrid Homo sapiens
79 CCAR2 57805
Affinity Capture-MS Homo sapiens
80 B4GALT2  
Affinity Capture-MS Homo sapiens
81 HIVEP1 3096
Two-hybrid Homo sapiens
82 COPG1 22820
Affinity Capture-MS Homo sapiens
83 ECT2L  
Affinity Capture-MS Homo sapiens
84 SIM2  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
FRET Homo sapiens
Affinity Capture-Western Homo sapiens
85 PNMA5 114824
Two-hybrid Homo sapiens
86 Tuba1a 22142
Two-hybrid Mus musculus
87 BRCA2 675
Two-hybrid Homo sapiens
88 UBR5 51366
Affinity Capture-MS Homo sapiens
89 HOXC9  
Two-hybrid Homo sapiens
90 BIRC8  
Two-hybrid Homo sapiens
91 NGFR 4804
Affinity Capture-Western Homo sapiens
92 NTRK1 4914
Affinity Capture-MS Homo sapiens
93 UBQLN2 29978
Two-hybrid Homo sapiens
94 GLI3 2737
Proximity Label-MS Homo sapiens
95 PLK1 5347
Two-hybrid Homo sapiens
96 MEOX2  
Two-hybrid Homo sapiens
97 HUWE1 10075
Affinity Capture-MS Homo sapiens
98 PLEKHG7  
Affinity Capture-MS Homo sapiens
99 HEMK1  
Two-hybrid Homo sapiens
100 PRR20E  
Two-hybrid Homo sapiens
101 IARS 3376
Affinity Capture-MS Homo sapiens
102 VENTX  
Two-hybrid Homo sapiens
103 MGAT5B  
Two-hybrid Homo sapiens
104 Ksr1  
Affinity Capture-MS Mus musculus
105 UNC5A  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
106 CERCAM 51148
Two-hybrid Homo sapiens
107 C6orf165  
Two-hybrid Homo sapiens
108 BARD1 580
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
109 BAG3 9531
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
110 CCDC59  
Affinity Capture-MS Homo sapiens
111 FOXH1  
Two-hybrid Homo sapiens
112 KRTAP19-5  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
113 NPAS4  
FRET Homo sapiens
Affinity Capture-Western Homo sapiens
114 AMBRA1  
Affinity Capture-MS Homo sapiens
115 TTC32  
Two-hybrid Homo sapiens
116 LMNB1 4001
Affinity Capture-MS Homo sapiens
117 RC3H2  
Affinity Capture-MS Homo sapiens
118 SIRT7  
Two-hybrid Homo sapiens
119 CCDC120  
Two-hybrid Homo sapiens
120 AKAP9 10142
Two-hybrid Homo sapiens
121 PRR20D  
Two-hybrid Homo sapiens
122 FOXI1  
Two-hybrid Homo sapiens
123 CUL3 8452
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
124 FOXD2  
Two-hybrid Homo sapiens
125 GATA5  
Two-hybrid Homo sapiens
126 BHLHE40  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
127 POM121  
Two-hybrid Homo sapiens
128 RBFOX1 54715
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
129 ARHGEF16 27237
Two-hybrid Homo sapiens
130 NAF1  
Two-hybrid Homo sapiens
131 RIPK4  
Affinity Capture-MS Homo sapiens
132 TTC23  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
133 TFG 10342
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
134 MPC1 51660
Two-hybrid Homo sapiens
135 CEP250 11190
Affinity Capture-MS Homo sapiens
136 MAPK6  
Affinity Capture-MS Homo sapiens
137 C12orf74  
Affinity Capture-MS Homo sapiens
138 GCN1L1 10985
Affinity Capture-MS Homo sapiens
139 PRR20B  
Two-hybrid Homo sapiens
140 GLYCTK  
Two-hybrid Homo sapiens
141 C1orf94  
Two-hybrid Homo sapiens
142 RXFP4  
Two-hybrid Homo sapiens
143 DHFRL1  
Proximity Label-MS Homo sapiens
144 PARP1 142
Proximity Label-MS Homo sapiens
145 KRTAP6-1  
Two-hybrid Homo sapiens
146 ILK 3611
Affinity Capture-MS Homo sapiens
147 PCM1 5108
Proximity Label-MS Homo sapiens
148 PRKAB2 5565
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
149 MAP3K7  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
150 NANOG  
Affinity Capture-MS Homo sapiens
151 CHUK 1147
Affinity Capture-Western Homo sapiens
152 EIF4E2  
Two-hybrid Homo sapiens
153 UBXN1 51035
Affinity Capture-MS Homo sapiens
154 KRTAP6-3  
Two-hybrid Homo sapiens
155 BAG4  
Two-hybrid Homo sapiens
156 FAM103A1  
Two-hybrid Homo sapiens
157 ACBD5 91452
Proximity Label-MS Homo sapiens
158 MTHFD1L 25902
Affinity Capture-MS Homo sapiens
159 MAPK1IP1L 93487
Two-hybrid Homo sapiens
160 RNF43  
Proximity Label-MS Homo sapiens
161 SMN1 6606
Two-hybrid Homo sapiens
162 NUP205 23165
Affinity Capture-MS Homo sapiens
163 PROP1  
Two-hybrid Homo sapiens
164 PRR20A  
Two-hybrid Homo sapiens
165 SIM1  
FRET Homo sapiens
Affinity Capture-Western Homo sapiens
166 DNAJA2 10294
Proximity Label-MS Homo sapiens
167 DAZAP2  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
168 DNAAF5 54919
Affinity Capture-MS Homo sapiens
169 PITX1  
Two-hybrid Homo sapiens
170 SQSTM1 8878
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
171 MKRN3  
Two-hybrid Homo sapiens
172 FANCI 55215
Affinity Capture-MS Homo sapiens
173 PJA2  
Two-hybrid Homo sapiens
174 SMN2 6607
Two-hybrid Homo sapiens
175 GPANK1  
Two-hybrid Homo sapiens
176 ROR2 4920
Affinity Capture-Western Homo sapiens
177 AGRN 375790
Two-hybrid Homo sapiens
178 PCNA 5111
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
179 DMRT2  
Two-hybrid Homo sapiens
180 MIF 4282
Affinity Capture-MS Homo sapiens
181 ERCC3  
Two-hybrid Homo sapiens
182 RBFOX2 23543
Two-hybrid Homo sapiens
183 NOC2L 26155
Affinity Capture-MS Homo sapiens
184 MAGED1 9500
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
185 EIF3J 8669
Two-hybrid Homo sapiens
186 CALML5 51806
Affinity Capture-MS Homo sapiens
187 BAG6 7917
Affinity Capture-MS Homo sapiens
188 RUSC1  
Two-hybrid Homo sapiens
189 FAM83A  
Two-hybrid Homo sapiens
190 RBPMS2  
Two-hybrid Homo sapiens
191 LONRF1  
Two-hybrid Homo sapiens
192 GPR135  
Two-hybrid Homo sapiens
193 C9orf72  
Affinity Capture-MS Homo sapiens
194 AR 367
Affinity Capture-MS Homo sapiens
195 ATXN3 4287
Affinity Capture-MS Homo sapiens
196 HSF2BP  
Two-hybrid Homo sapiens
197 NEDD8 4738
Affinity Capture-MS Homo sapiens
198 EP300 2033
Two-hybrid Homo sapiens
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