Gene description for EXOC5
Gene name exocyst complex component 5
Gene symbol EXOC5
Other names/aliases HSEC10
PRO1912
SEC10
SEC10L1
SEC10P
Species Homo sapiens
 Database cross references - EXOC5
ExoCarta ExoCarta_10640
Vesiclepedia VP_10640
Entrez Gene 10640
HGNC 10696
MIM 604469
UniProt O00471  
 EXOC5 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocellular carcinoma cells 26054723    
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for EXOC5
Molecular Function
    protein binding GO:0005515 IPI
    small GTPase binding GO:0031267 IPI
Biological Process
    mitotic cytokinesis GO:0000281 NAS
    establishment of planar polarity GO:0001736 IEA
    exocytosis GO:0006887 IBA
    post-Golgi vesicle-mediated transport GO:0006892 TAS
    Golgi to plasma membrane transport GO:0006893 IBA
    vesicle docking involved in exocytosis GO:0006904 NAS
    protein transport GO:0015031 IEA
    protein localization to plasma membrane GO:0072659 IEA
    membrane fission GO:0090148 NAS
    vesicle tethering involved in exocytosis GO:0090522 NAS
    epithelial cell apoptotic process GO:1904019 IEA
    non-motile cilium assembly GO:1905515 IEA
Subcellular Localization
    exocyst GO:0000145 IBA
    exocyst GO:0000145 NAS
    cytoplasm GO:0005737 TAS
    cytosol GO:0005829 TAS
    midbody GO:0030496 IEA
 Experiment description of studies that identified EXOC5 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 234
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-C3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
6
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for EXOC5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CBX1 10951
Affinity Capture-MS Homo sapiens
2 TBX21  
Two-hybrid Homo sapiens
3 C9orf78 51759
Affinity Capture-MS Homo sapiens
4 TTC23L  
Two-hybrid Homo sapiens
5 EDNRB 1910
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 SETDB1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 PNKD  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 SLC18A2  
Affinity Capture-MS Homo sapiens
9 EXOC1 55763
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
10 ENO2 2026
Two-hybrid Homo sapiens
11 CDH2 1000
Two-hybrid Homo sapiens
12 OPRM1 4988
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 SEMA4C 54910
Affinity Capture-MS Homo sapiens
14 GGA1 26088
Affinity Capture-MS Homo sapiens
15 EPHA1 2041
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 TACSTD2 4070
Affinity Capture-MS Homo sapiens
17 ITFG3 83986
Affinity Capture-MS Homo sapiens
18 SCHIP1  
Affinity Capture-MS Homo sapiens
19 CA14 23632
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 EXOC7 23265
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
21 EXOC5 10640
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
22 GPR17 2840
Affinity Capture-MS Homo sapiens
23 EXOC3 11336
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
24 IFT20 90410
Affinity Capture-MS Homo sapiens
25 EXOC2 55770
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
26 CD70 970
Affinity Capture-MS Homo sapiens
27 GCGR  
Affinity Capture-MS Homo sapiens
28 RAB14 51552
Two-hybrid Homo sapiens
29 PARK2  
Affinity Capture-MS Homo sapiens
30 CHCHD3 54927
Affinity Capture-MS Homo sapiens
31 IL1R2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 DTNBP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 EXOC4 60412
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
34 RAB4A 5867
Two-hybrid Homo sapiens
35 PRKCG 5582
Two-hybrid Homo sapiens
36 PTK6  
Two-hybrid Homo sapiens
37 DNAJC5 80331
Proximity Label-MS Homo sapiens
38 ACTR3B 57180
Two-hybrid Homo sapiens
39 TNPO2 30000
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
40 BARD1 580
Two-hybrid Homo sapiens
41 NTRK1 4914
Affinity Capture-MS Homo sapiens
42 CCDC102B  
Two-hybrid Homo sapiens
43 GPR182  
Affinity Capture-MS Homo sapiens
44 EXOC6 54536
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
45 ERP29 10961
Affinity Capture-MS Homo sapiens
46 EXOC6B 23233
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
47 HECTD1 25831
Affinity Capture-MS Homo sapiens
48 RAB4B 53916
Two-hybrid Homo sapiens
49 TXNL4A 10907
Two-hybrid Homo sapiens
50 TXLNB  
Two-hybrid Homo sapiens
51 SEC61B 10952
Affinity Capture-Western Homo sapiens
52 ADCK1  
Two-hybrid Homo sapiens
53 ZNF609  
Affinity Capture-MS Homo sapiens
54 OAS1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
55 DSN1 79980
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 CD40 958
Affinity Capture-MS Homo sapiens
57 WHAMMP3  
Affinity Capture-MS Homo sapiens
58 HDDC3  
Two-hybrid Homo sapiens
59 YWHAZ 7534
Affinity Capture-MS Homo sapiens
60 GYPA  
Affinity Capture-MS Homo sapiens
61 MTX2 10651
Affinity Capture-MS Homo sapiens
62 NPTN 27020
Affinity Capture-MS Homo sapiens
63 IQCB1  
Affinity Capture-MS Homo sapiens
64 C9orf9  
Two-hybrid Homo sapiens
65 CMKLR1 1240
Affinity Capture-MS Homo sapiens
66 CD274 29126
Affinity Capture-MS Homo sapiens
67 CUL3 8452
Affinity Capture-MS Homo sapiens
68 PTEN 5728
Affinity Capture-MS Homo sapiens
69 STIM1 6786
Affinity Capture-MS Homo sapiens
70 ICA1  
Two-hybrid Homo sapiens
71 NPY2R  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 EGFR 1956
Affinity Capture-MS Homo sapiens
73 ARF6 382
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
74 BRMS1L  
Two-hybrid Homo sapiens
75 COG6 57511
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 C3orf18  
Affinity Capture-MS Homo sapiens
77 RPA3 6119
Proximity Label-MS Homo sapiens
78 GPR45  
Affinity Capture-MS Homo sapiens
79 SCN2B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
80 MAGEA6  
Two-hybrid Homo sapiens
81 SOX10  
Two-hybrid Homo sapiens
82 KRT222 125113
Affinity Capture-MS Homo sapiens
83 FRMD6 122786
Two-hybrid Homo sapiens
84 STX11 8676
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
85 DVL2 1856
Affinity Capture-MS Homo sapiens
86 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
87 NSF 4905
Affinity Capture-MS Homo sapiens
88 EXOC8 149371
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
89 PYCARD 29108
Two-hybrid Homo sapiens
90 LCK 3932
Proximity Label-MS Homo sapiens
91 EFNB1 1947
Affinity Capture-MS Homo sapiens
92 CREBBP  
Two-hybrid Homo sapiens
93 EXOC3L1  
Affinity Capture-MS Homo sapiens
94 RAB8A 4218
Affinity Capture-MS Homo sapiens
95 ATP5B 506
Co-fractionation Homo sapiens
96 RAB11A 8766
Proximity Label-MS Homo sapiens
97 KXD1 79036
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
98 SNW1 22938
Affinity Capture-MS Homo sapiens
99 EDRF1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
100 CCDC67  
Two-hybrid Homo sapiens
101 LURAP1  
Affinity Capture-MS Homo sapiens
102 CDC5L 988
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here