Gene description for PIAS1
Gene name protein inhibitor of activated STAT, 1
Gene symbol PIAS1
Other names/aliases DDXBP1
Species Homo sapiens
 Database cross references - PIAS1
ExoCarta ExoCarta_8554
Entrez Gene 8554
HGNC 2752
MIM 603566
UniProt O75925  
 PIAS1 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for PIAS1
Molecular Function
    SUMO ligase activity GO:0061665 IDA
    transcription coactivator activity GO:0003713 NAS
    protein binding GO:0005515 IPI
    DNA binding GO:0003677 IEA
    enzyme binding GO:0019899 IPI
    ubiquitin protein ligase binding GO:0031625 IEA
    zinc ion binding GO:0008270 IEA
    androgen receptor binding GO:0050681 NAS
    protein domain specific binding GO:0019904 IEA
    ligase activity GO:0016874 IEA
    transcription corepressor activity GO:0003714 TAS
Biological Process
    positive regulation of smooth muscle cell differentiation GO:0051152 IEA
    positive regulation of transcription, DNA-templated GO:0045893 NAS
    interferon-gamma-mediated signaling pathway GO:0060333 TAS
    JAK-STAT cascade GO:0007259 TAS
    protein-DNA complex assembly GO:0065004 IEA
    protein sumoylation GO:0016925 TAS
    regulation of interferon-gamma-mediated signaling pathway GO:0060334 TAS
    cytokine-mediated signaling pathway GO:0019221 TAS
    positive regulation of protein sumoylation GO:0033235 IDA
    androgen receptor signaling pathway GO:0030521 NAS
    negative regulation of transcription from RNA polymerase II promoter GO:0000122 IEA
    regulation of cell proliferation GO:0042127 ISS
    post-translational protein modification GO:0043687 TAS
    cellular protein metabolic process GO:0044267 TAS
    positive regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032436 ISS
    transcription, DNA-templated GO:0006351 IEA
    spermatogenesis GO:0007283 IEA
Subcellular Localization
    nuclear speck GO:0016607 IEA
    PML body GO:0016605 ISS
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 TAS
 Experiment description of studies that identified PIAS1 in exosomes
Experiment ID 236
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for PIAS1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DNMT3A 1788
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
2 CSRP2 1466
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
3 UBE2I 7329
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
4 STAB2 55576
Affinity Capture-Western Homo sapiens
5 STAT1 6772
Invitro Homo sapiens
Two-hybrid Homo sapiens
6 SP3  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
7 TP53  
Two-hybrid Homo sapiens
8 SUMO1 7341
Invitro Homo sapiens
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which PIAS1 is involved
Regulation of IFNG signaling TAS Reactome
SUMOylation of DNA damage response and repair proteins TAS Reactome

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