Gene description for NOL9
Gene name nucleolar protein 9
Gene symbol NOL9
Other names/aliases Grc3
NET6
Species Homo sapiens
 Database cross references - NOL9
ExoCarta ExoCarta_79707
Vesiclepedia VP_79707
Entrez Gene 79707
HGNC 26265
UniProt Q5SY16  
 NOL9 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for NOL9
Molecular Function
    RNA binding GO:0003723 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 TAS
    ATP-dependent polydeoxyribonucleotide 5'-hydroxyl-kinase activity GO:0046404 IDA
    polynucleotide 5'-hydroxyl-kinase activity GO:0051731 IBA
    ATP-dependent polyribonucleotide 5'-hydroxyl-kinase activity GO:0051736 IEA
Biological Process
    cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) GO:0000448 IBA
    maturation of 5.8S rRNA GO:0000460 IMP
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleoplasm GO:0005654 TAS
    nucleolus GO:0005730 IDA
    nucleolus GO:0005730 TAS
    membrane GO:0016020 HDA
    intermediate filament cytoskeleton GO:0045111 IDA
 Experiment description of studies that identified NOL9 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for NOL9
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Kif19a  
Affinity Capture-MS Mus musculus
2 RIT1 6016
Negative Genetic Homo sapiens
3 SENP3 26168
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 DNAJC9 23234
Proximity Label-MS Homo sapiens
5 REL 5966
Two-hybrid Homo sapiens
6 HIST1H2BG 8339
Proximity Label-MS Homo sapiens
7 NOP56 10528
Proximity Label-MS Homo sapiens
8 C9orf78 51759
Affinity Capture-MS Homo sapiens
9 CENPA  
Proximity Label-MS Homo sapiens
10 DNAJB6 10049
Proximity Label-MS Homo sapiens
11 USP36  
Affinity Capture-MS Homo sapiens
12 UBE2O 63893
Affinity Capture-MS Homo sapiens
13 CHCHD2  
Affinity Capture-MS Homo sapiens
14 NPM1 4869
Affinity Capture-MS Homo sapiens
15 POLR1E  
Proximity Label-MS Homo sapiens
16 DNAJB1 3337
Proximity Label-MS Homo sapiens
17 MRS2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 BRD4 23476
Affinity Capture-MS Homo sapiens
19 ATG7 10533
Affinity Capture-MS Homo sapiens
20 DNAJC7 7266
Proximity Label-MS Homo sapiens
21 SPIN3  
Affinity Capture-MS Homo sapiens
22 IMPDH1 3614
Affinity Capture-MS Homo sapiens
23 PHF10  
Affinity Capture-MS Homo sapiens
24 PPP5C 5536
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
25 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
26 PARK2  
Affinity Capture-MS Homo sapiens
27 DNAJB8  
Proximity Label-MS Homo sapiens
28 ZNF638 27332
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 BARD1 580
Affinity Capture-MS Homo sapiens
30 NTRK1 4914
Affinity Capture-MS Homo sapiens
31 RPL31 6160
Proximity Label-MS Homo sapiens
32 LAS1L 81887
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 SUMO2 6613
Reconstituted Complex Homo sapiens
34 JPH1 56704
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 PML 5371
Affinity Capture-MS Homo sapiens
36 HECTD1 25831
Affinity Capture-MS Homo sapiens
37 SHCBP1 79801
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 PELP1 27043
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 RANBP6 26953
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 CD3EAP  
Proximity Label-MS Homo sapiens
41 ZBTB2 57621
Affinity Capture-MS Homo sapiens
42 IPO5 3843
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 RPS2 6187
Affinity Capture-MS Homo sapiens
44 XPO1 7514
Affinity Capture-MS Homo sapiens
45 KCTD9  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 SIRT7  
Affinity Capture-MS Homo sapiens
47 AHNAK 79026
Two-hybrid Homo sapiens
48 NPM3 10360
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 CUL3 8452
Affinity Capture-MS Homo sapiens
50 CIT 11113
Affinity Capture-MS Homo sapiens
51 FOXL1  
Affinity Capture-MS Homo sapiens
52 PSPC1 55269
Affinity Capture-MS Homo sapiens
53 RPA3 6119
Proximity Label-MS Homo sapiens
54 LTV1  
Affinity Capture-MS Homo sapiens
55 H2AFB1  
Affinity Capture-MS Homo sapiens
56 RBM14-RBM4 100526737
Affinity Capture-MS Homo sapiens
57 Mllt1  
Affinity Capture-MS Mus musculus
58 DDRGK1 65992
Affinity Capture-MS Homo sapiens
59 RNF2  
Affinity Capture-MS Homo sapiens
60 BMI1  
Affinity Capture-MS Homo sapiens
61 FOXQ1  
Affinity Capture-MS Homo sapiens
62 HAX1  
Proximity Label-MS Homo sapiens
63 NEUROG3  
Affinity Capture-MS Homo sapiens
64 PASK  
Affinity Capture-MS Homo sapiens
65 VPRBP 9730
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 KIAA0232 9778
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
68 PARP1 142
Proximity Label-MS Homo sapiens
69 TP53 7157
Affinity Capture-MS Homo sapiens
70 MNDA 4332
Affinity Capture-MS Homo sapiens
71 DNAJA2 10294
Affinity Capture-MS Homo sapiens
72 2310003H01Rik  
Affinity Capture-MS Mus musculus
73 FYCO1 79443
Affinity Capture-MS Homo sapiens
74 NIFK 84365
Proximity Label-MS Homo sapiens
75 WDR18 57418
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 TCF4  
Two-hybrid Homo sapiens
77 RPS24 6229
Proximity Label-MS Homo sapiens
78 Samhd1 56045
Affinity Capture-MS Mus musculus
79 RPS6 6194
Proximity Label-MS Homo sapiens
80 C1QBP 708
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
81 PTGES3 10728
Affinity Capture-MS Homo sapiens
82 KRAS 3845
Negative Genetic Homo sapiens
83 C9orf72  
Affinity Capture-MS Homo sapiens
84 SRP9 6726
Affinity Capture-MS Homo sapiens
85 TEX10 54881
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
86 C5orf22  
Affinity Capture-MS Homo sapiens
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