Gene description for STAT1
Gene name signal transducer and activator of transcription 1, 91kDa
Gene symbol STAT1
Other names/aliases CANDF7
IMD31A
IMD31B
IMD31C
ISGF-3
STAT91
Species Homo sapiens
 Database cross references - STAT1
ExoCarta ExoCarta_6772
Entrez Gene 6772
HGNC 11362
MIM 600555
UniProt P42224  
 STAT1 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Endothelial cells 26027894    
Mesenchymal stem cells Unpublished / Not applicable
Nasopharyngeal carcinoma cells 25857718    
Nasopharyngeal carcinoma cells 25857718    
Nasopharyngeal carcinoma cells 25857718    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Squamous carcinoma cells 20124223    
Thymus 23844026    
 Gene ontology annotations for STAT1
Molecular Function
    identical protein binding GO:0042802 IPI
    RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity GO:0000983 IDA
    enzyme binding GO:0019899 IPI
    double-stranded DNA binding GO:0003690 IDA
    RNA polymerase II core promoter proximal region sequence-specific DNA binding GO:0000978 IDA
    signal transducer activity GO:0004871 IEA
    RNA polymerase II core promoter sequence-specific DNA binding GO:0000979 IDA
    protein homodimerization activity GO:0042803 IDA
    CCR5 chemokine receptor binding GO:0031730 IEA
    tumor necrosis factor receptor binding GO:0005164 IPI
    protein binding GO:0005515 IPI
    sequence-specific DNA binding transcription factor activity GO:0003700 IDA
Biological Process
    response to hydrogen peroxide GO:0042542 IEA
    blood circulation GO:0008015 ISS
    positive regulation of smooth muscle cell proliferation GO:0048661 ISS
    metanephric mesenchymal cell proliferation involved in metanephros development GO:0072136 ISS
    cellular response to interferon-beta GO:0035458 IMP
    regulation of type I interferon-mediated signaling pathway GO:0060338 TAS
    renal tubule development GO:0061326 IMP
    viral process GO:0016032 IEA
    response to mechanical stimulus GO:0009612 IEA
    lipopolysaccharide-mediated signaling pathway GO:0031663 IEA
    positive regulation of mesenchymal cell proliferation GO:0002053 ISS
    regulation of apoptotic process GO:0042981 TAS
    transcription from RNA polymerase II promoter GO:0006366 IDA
    response to peptide hormone GO:0043434 ISS
    negative regulation of angiogenesis GO:0016525 IMP
    regulation of interferon-gamma-mediated signaling pathway GO:0060334 TAS
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 IMP
    negative regulation of metanephric nephron tubule epithelial cell differentiation GO:0072308 ISS
    response to exogenous dsRNA GO:0043330 IEA
    endothelial cell migration GO:0043542 IMP
    tumor necrosis factor-mediated signaling pathway GO:0033209 IDA
    JAK-STAT cascade involved in growth hormone signaling pathway GO:0060397 TAS
    metanephric mesenchymal cell differentiation GO:0072162 ISS
    negative regulation of endothelial cell proliferation GO:0001937 IMP
    negative regulation of macrophage fusion GO:0034240 IEA
    interferon-gamma-mediated signaling pathway GO:0060333 TAS
    negative regulation of transcription from RNA polymerase II promoter GO:0000122 ISS
    negative regulation by virus of viral protein levels in host cell GO:0046725 IMP
    negative regulation of I-kappaB kinase/NF-kappaB signaling GO:0043124 IMP
    regulation of transcription from RNA polymerase II promoter GO:0006357 IDA
    positive regulation of transcription, DNA-templated GO:0045893 IDA
    JAK-STAT cascade GO:0007259 IDA
    type I interferon signaling pathway GO:0060337 TAS
    defense response to virus GO:0051607 IEA
    cellular response to insulin stimulus GO:0032869 IEA
    response to cAMP GO:0051591 ISS
    cytokine-mediated signaling pathway GO:0019221 TAS
    apoptotic process GO:0006915 ISS
    response to nutrient GO:0007584 IEA
    negative regulation of mesenchymal to epithelial transition involved in metanephros morphogenesis GO:0003340 ISS
    response to cytokine GO:0034097 ISS
    response to drug GO:0042493 IEA
Subcellular Localization
    nucleoplasm GO:0005654 TAS
    dendrite GO:0030425 ISS
    perinuclear region of cytoplasm GO:0048471 IDA
    axon GO:0030424 ISS
    nucleus GO:0005634 IDA
    cytosol GO:0005829 TAS
    cytoplasm GO:0005737 IDA
    nucleolus GO:0005730 IDA
    nuclear chromatin GO:0000790 IDA
 Experiment description of studies that identified STAT1 in exosomes
1
Experiment ID 207
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
ISEV standards
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 226
ISEV standards
EM
EV Biophysical techniques
GAPDH
EV Cytosolic markers
CD9|FLOT1
EV Membrane markers
LMNA|H2AFX|ATP5A1|TOMM20
EV Negative markers
EV Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting
Authors Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
5
Experiment ID 126
ISEV standards
EV Biophysical techniques
GAPDH
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
6
Experiment ID 219
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD63|CD9
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25857718    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from nasopharyngeal carcinoma cell identifies intercellular transfer of angiogenic proteins
Authors Chan YK, Zhang H, Liu P, Tsao GS, Li Lung M, Mak NK, Ngok-Shun Wong R, Ying-Kit Yue P
Journal name Int J Cancer
Publication year 2015
Sample Nasopharyngeal carcinoma cells
Sample name C666-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.17-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 220
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD63|CD9
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25857718    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from nasopharyngeal carcinoma cell identifies intercellular transfer of angiogenic proteins
Authors Chan YK, Zhang H, Liu P, Tsao GS, Li Lung M, Mak NK, Ngok-Shun Wong R, Ying-Kit Yue P
Journal name Int J Cancer
Publication year 2015
Sample Nasopharyngeal carcinoma cells
Sample name NP69
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.17-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 221
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD63|CD9
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25857718    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from nasopharyngeal carcinoma cell identifies intercellular transfer of angiogenic proteins
Authors Chan YK, Zhang H, Liu P, Tsao GS, Li Lung M, Mak NK, Ngok-Shun Wong R, Ying-Kit Yue P
Journal name Int J Cancer
Publication year 2015
Sample Nasopharyngeal carcinoma cells
Sample name NP460
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.17-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 191
ISEV standards
EV Biophysical techniques
Alix
EV Cytosolic markers
CD81|CD9
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
12
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for STAT1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ISGF3G  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
2 MCM3 4172
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
3 VDR  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
4 FANCC 2176
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
5 STAT2 6773
Invivo Homo sapiens
Co-purification Homo sapiens
6 PRKCD 5580
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
7 KPNA1 3836
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
8 NMI  
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
9 XPO1 7514
Invitro Homo sapiens
10 MDK 4192
Affinity Capture-MS Homo sapiens
11 GTF2I 2969
Invivo Homo sapiens
Invitro Homo sapiens
12 MCM5 4174
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
13 EIF2AK2 5610
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
14 IL2RG  
Affinity Capture-MS Homo sapiens
15 BRCA1 672
Invivo Homo sapiens
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
16 TNFRSF1A 7132
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
17 TRADD 8717
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
18 EGFR 1956
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
19 CREBBP  
Reconstituted Complex Homo sapiens
Phenotypic Enhancement Homo sapiens
20 PTK2 5747
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
21 ELP2 55250
Reconstituted Complex Homo sapiens
22 IFNAR2  
Reconstituted Complex Homo sapiens
23 JUN 3725
Invivo Homo sapiens
24 LMO2  
Two-hybrid Homo sapiens
25 UBE2I 7329
Affinity Capture-MS Homo sapiens
26 STAT1 6772
Affinity Capture-MS Homo sapiens
Co-purification Homo sapiens
Two-hybrid Homo sapiens
Co-crystal Structure Homo sapiens
Affinity Capture-MS Homo sapiens
Co-purification Homo sapiens
Two-hybrid Homo sapiens
Co-crystal Structure Homo sapiens
27 PIAS1 8554
Invitro Homo sapiens
Two-hybrid Homo sapiens
28 C20orf185  
Affinity Capture-MS Homo sapiens
29 TYK2 7297
Biochemical Activity Homo sapiens
30 HSF1  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
31 MTOR 2475
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
32 MGC11102  
Two-hybrid Homo sapiens
33 IL27RA 9466
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
34 IL2RB  
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
35 SRC 6714
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
36 STAT3 6774
Invivo Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
37 GNB2L1 10399
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
38 BMX  
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
39 IRF1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
40 CAMK2G 818
Invivo Homo sapiens
Invitro Homo sapiens
41 EP300 2033
Affinity Capture-Western Homo sapiens
42 KIT 3815
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
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