Gene description for Actb
Gene name actin, beta
Gene symbol Actb
Other names/aliases Actx
E430023M04Rik
beta-actin
Species Mus musculus
 Database cross references - Actb
ExoCarta ExoCarta_11461
Vesiclepedia VP_11461
Entrez Gene 11461
UniProt P60710  
 Actb identified in exosomes derived from the following tissue/cell type
Brain cancer cells 19109410    
Colon cancer cells 37309723    
Colorectal cancer cells 33431899    
Colorectal cancer cells 33431899    
Dendritic cells 10545503    
Embryonic fibroblasts 18494037    
Faeces 33431899    
Faeces 33431899    
Fibroblasts 23260141    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
Mast cells 11145662    
Mast cells 17486113    
Melanoma cells 34957415    
Melanoma cells 34957415    
Mov neuroglial cells 15210972    
Neural stem cells 25242146    
Neural stem cells 25242146    
Neural stem cells 25242146    
Oligodendrocytes 21136642    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
Tracheobronchial cells 19190083    
 Gene ontology annotations for Actb
Molecular Function
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ATP hydrolysis activity GO:0016887 IEA
    ATP hydrolysis activity GO:0016887 ISO
    kinesin binding GO:0019894 IEA
    kinesin binding GO:0019894 ISO
    protein kinase binding GO:0019901 IBA
    protein kinase binding GO:0019901 IEA
    protein kinase binding GO:0019901 ISO
    Tat protein binding GO:0030957 IEA
    Tat protein binding GO:0030957 ISO
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
    nitric-oxide synthase binding GO:0050998 IEA
    nitric-oxide synthase binding GO:0050998 ISO
    structural constituent of postsynaptic actin cytoskeleton GO:0098973 IBA
    structural constituent of postsynaptic actin cytoskeleton GO:0098973 IEA
    structural constituent of postsynaptic actin cytoskeleton GO:0098973 ISO
Biological Process
    morphogenesis of a polarized epithelium GO:0001738 IEA
    morphogenesis of a polarized epithelium GO:0001738 ISO
    chromatin remodeling GO:0006338 NAS
    regulation of transcription by RNA polymerase II GO:0006357 NAS
    establishment or maintenance of cell polarity GO:0007163 IEA
    establishment or maintenance of cell polarity GO:0007163 ISO
    axonogenesis GO:0007409 IBA
    axonogenesis GO:0007409 ISO
    positive regulation of cell population proliferation GO:0008284 NAS
    regulation of transmembrane transporter activity GO:0022898 ISO
    regulation of mitotic metaphase/anaphase transition GO:0030071 NAS
    adherens junction assembly GO:0034333 IEA
    adherens junction assembly GO:0034333 ISO
    regulation of apoptotic process GO:0042981 NAS
    apical protein localization GO:0045176 IEA
    apical protein localization GO:0045176 ISO
    positive regulation of T cell differentiation GO:0045582 NAS
    negative regulation of cell differentiation GO:0045596 NAS
    positive regulation of cell differentiation GO:0045597 NAS
    positive regulation of myoblast differentiation GO:0045663 NAS
    positive regulation of DNA-templated transcription GO:0045893 NAS
    cell motility GO:0048870 IBA
    cell motility GO:0048870 IEA
    cell motility GO:0048870 ISO
    regulation of norepinephrine uptake GO:0051621 IEA
    regulation of norepinephrine uptake GO:0051621 ISO
    regulation of cell cycle GO:0051726 ISO
    regulation of G0 to G1 transition GO:0070316 NAS
    cellular response to electrical stimulus GO:0071257 ISO
    protein localization to adherens junction GO:0071896 IEA
    protein localization to adherens junction GO:0071896 ISO
    cellular response to cytochalasin B GO:0072749 IEA
    cellular response to cytochalasin B GO:0072749 ISO
    postsynaptic actin cytoskeleton organization GO:0098974 IEA
    postsynaptic actin cytoskeleton organization GO:0098974 ISO
    regulation of transepithelial transport GO:0150111 IEA
    regulation of transepithelial transport GO:0150111 ISO
    regulation of synaptic vesicle endocytosis GO:1900242 IDA
    regulation of synaptic vesicle endocytosis GO:1900242 IMP
    positive regulation of stem cell population maintenance GO:1902459 NAS
    regulation of protein localization to plasma membrane GO:1903076 IEA
    regulation of protein localization to plasma membrane GO:1903076 ISO
    positive regulation of double-strand break repair via homologous recombination GO:1905168 ISO
    regulation of G1/S transition of mitotic cell cycle GO:2000045 NAS
    regulation of double-strand break repair GO:2000779 NAS
    positive regulation of double-strand break repair GO:2000781 NAS
    regulation of nucleotide-excision repair GO:2000819 NAS
Subcellular Localization
    kinetochore GO:0000776 NAS
    chromatin GO:0000785 NAS
    nucleosome GO:0000786 ISO
    stress fiber GO:0001725 ISO
    podosome GO:0002102 ISO
    nucleus GO:0005634 IDA
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    cytoskeleton GO:0005856 IDA
    cytoskeleton GO:0005856 ISO
    actin filament GO:0005884 IBA
    plasma membrane GO:0005886 ISS
    brush border GO:0005903 IDA
    cell-cell junction GO:0005911 ISO
    adherens junction GO:0005912 IEA
    adherens junction GO:0005912 ISO
    focal adhesion GO:0005925 ISS
    actin cytoskeleton GO:0015629 IBA
    actin cytoskeleton GO:0015629 ISO
    actin cytoskeleton GO:0015629 ISS
    membrane GO:0016020 IBA
    nuclear matrix GO:0016363 NAS
    SWI/SNF complex GO:0016514 NAS
    RSC-type complex GO:0016586 NAS
    lamellipodium GO:0030027 IEA
    lamellipodium GO:0030027 ISO
    axon GO:0030424 IBA
    axon GO:0030424 ISO
    cortical cytoskeleton GO:0030863 IDA
    protein-containing complex GO:0032991 ISO
    protein-containing complex GO:0032991 ISS
    brahma complex GO:0035060 NAS
    NuA4 histone acetyltransferase complex GO:0035267 IBA
    NuA4 histone acetyltransferase complex GO:0035267 ISO
    cytoplasmic ribonucleoprotein granule GO:0036464 IEA
    cytoplasmic ribonucleoprotein granule GO:0036464 ISO
    myelin sheath GO:0043209 HDA
    apical junction complex GO:0043296 IEA
    apical junction complex GO:0043296 ISO
    calyx of Held GO:0044305 IDA
    calyx of Held GO:0044305 IMP
    membrane raft GO:0045121 ISO
    synapse GO:0045202 IBA
    tight junction GO:0070160 IEA
    tight junction GO:0070160 ISO
    npBAF complex GO:0071564 NAS
    nBAF complex GO:0071565 NAS
    dense body GO:0097433 ISS
    Schaffer collateral - CA1 synapse GO:0098685 IDA
    Schaffer collateral - CA1 synapse GO:0098685 IMP
    postsynaptic actin cytoskeleton GO:0098871 IEA
    postsynaptic actin cytoskeleton GO:0098871 ISO
    glutamatergic synapse GO:0098978 IEA
    glutamatergic synapse GO:0098978 ISO
    bBAF complex GO:0140092 NAS
    GBAF complex GO:0140288 NAS
    ribonucleoprotein complex GO:1990904 IEA
    ribonucleoprotein complex GO:1990904 ISO
 Experiment description of studies that identified Actb in exosomes
1
Experiment ID 26
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|GAPDH|HSP70|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19109410    
Organism Mus musculus
Experiment description Proteomic and immunologic analyses of brain tumor exosomes.
Authors "Graner MW, Alzate O, Dechkovskaia AM, Keene JD, Sampson JH, Mitchell DA, Bigner DD"
Journal name FASEB
Publication year 2008
Sample Brain cancer cells
Sample name SMA560vIII
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.13-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF/TOF]
Western blotting
2
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 311
MISEV standards
✘ Biophysical techniques
✔
Cd63|Cd9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 33431899    
Organism Mus musculus
Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance
Authors "Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG."
Journal name Nat Commun
Publication year 2021
Sample Colorectal cancer cells
Sample name MC38
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
4
Experiment ID 312
MISEV standards
✘ Biophysical techniques
✔
Cd63|Cd9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 33431899    
Organism Mus musculus
Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance
Authors "Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG."
Journal name Nat Commun
Publication year 2021
Sample Colorectal cancer cells
Sample name MC38
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
5
Experiment ID 3
MISEV standards
✔
IEM
Biophysical techniques
✔
HSP84|HSC73|MHCII|CD9|mac-1
Enriched markers
✔
CANX|HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 10545503    
Organism Mus musculus
Experiment description Molecular characterization of dendritic cell-derived exosomes. Selective accumulation of the heat shock protein hsc73.
Authors "Thery C, Regnault A, Garin J, Wolfers J, Zitvogel L, Ricciardi-Castagnoli P, Raposo G, Amigorena S."
Journal name JCB
Publication year 1999
Sample Dendritic cells
Sample name D1
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.20 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
6
Experiment ID 73
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 18494037    
Organism Mus musculus
Experiment description Difference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes.
Authors "Ji H, Erfani N, Tauro BJ, Kapp EA, Zhu HJ, Moritz RL, Lim JW, Simpson RJ"
Journal name ELEC
Publication year 2008
Sample Embryonic fibroblasts
Sample name NIH3T3
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA]
Western blotting
7
Experiment ID 303
MISEV standards
✔
EM
Biophysical techniques
✔
Cd63|Cd81|Cd9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 33431899    
Organism Mus musculus
Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance
Authors "Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG."
Journal name Nat Commun
Publication year 2021
Sample Faeces
Sample name Intestinal epithelial cells - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Lipid
miRNA
Methods used in the study Western blotting
Mass spectrometry
Immunofluorescence
miRNA array
HPLC
8
Experiment ID 303
MISEV standards
✔
EM
Biophysical techniques
✔
Cd63|Cd81|Cd9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33431899    
Organism Mus musculus
Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance
Authors "Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG."
Journal name Nat Commun
Publication year 2021
Sample Faeces
Sample name Intestinal epithelial cells - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Lipid
miRNA
Methods used in the study Western blotting
Mass spectrometry
Immunofluorescence
miRNA array
HPLC
9
Experiment ID 304
MISEV standards
✔
EM
Biophysical techniques
✔
Cd63|Cd81|Cd9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 33431899    
Organism Mus musculus
Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance
Authors "Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG."
Journal name Nat Commun
Publication year 2021
Sample Faeces
Sample name Intestinal epithelial cells - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Lipid
miRNA
Methods used in the study Western blotting
Mass spectrometry
Immunofluorescence
miRNA array
HPLC
10
Experiment ID 304
MISEV standards
✔
EM
Biophysical techniques
✔
Cd63|Cd81|Cd9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33431899    
Organism Mus musculus
Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance
Authors "Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG."
Journal name Nat Commun
Publication year 2021
Sample Faeces
Sample name Intestinal epithelial cells - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Lipid
miRNA
Methods used in the study Western blotting
Mass spectrometry
Immunofluorescence
miRNA array
HPLC
11
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
12
Experiment ID 214
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
13
Experiment ID 215
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
14
Experiment ID 216
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
15
Experiment ID 5
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
CD86|CD80
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 11145662    
Organism Mus musculus
Experiment description Mast cell-dependent B and T lymphocyte activation is mediated by the secretion of immunologically active exosomes.
Authors "Skokos D, Le Panse S, Villa I, Rousselle JC, Peronet R, David B, Namane A, Mecheri S"
Journal name JIMMU
Publication year 2001
Sample Mast cells
Sample name P815
MC9
Bone marrow-derived mast cells
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF]
ELISA
16
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
17
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
18
Experiment ID 1117
MISEV standards
✘ Biophysical techniques
✔
Cd81|Icam1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1R2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
19
Experiment ID 1118
MISEV standards
✘ Biophysical techniques
✔
Cd81|Icam1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1R2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
20
Experiment ID 39
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG101|HSC70|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15210972    
Organism Mus musculus
Experiment description Cells release prions in association with exosomes.
Authors "Fevrier B, Vilette D, Archer F, Loew D, Faigle W, Vidal M, Laude H, Raposo G"
Journal name PNAS
Publication year 2004
Sample Mov neuroglial cells
Sample name Mov neuroglial cell
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
21
Experiment ID 262
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|TSG101|HSP70|HSP90|CD63|CD9|
Enriched markers
✘ Negative markers
✔
Y
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 25242146    
Organism Mus musculus
Experiment description Extracellular Vesicles from Neural Stem Cells Transfer IFN-? via Ifngr1 to Activate Stat1 Signaling in Target Cells
Authors "Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino"
Journal name Molecular Cell
Publication year 2014
Sample Neural stem cells
Sample name NPCs
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.20 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
Microarray
22
Experiment ID 263
MISEV standards
✘ Biophysical techniques
✔
CD63|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 25242146    
Organism Mus musculus
Experiment description Extracellular Vesicles from Neural Stem Cells Transfer IFN-? via Ifngr1 to Activate Stat1 Signaling in Target Cells
Authors "Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino"
Journal name Molecular Cell
Publication year 2014
Sample Neural stem cells
Sample name NPCs - Th1 treated
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.20 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
23
Experiment ID 264
MISEV standards
✘ Biophysical techniques
✔
CD63|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 25242146    
Organism Mus musculus
Experiment description Extracellular Vesicles from Neural Stem Cells Transfer IFN-? via Ifngr1 to Activate Stat1 Signaling in Target Cells
Authors "Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino"
Journal name Molecular Cell
Publication year 2014
Sample Neural stem cells
Sample name NPCs - Th2 treated
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.20 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
24
Experiment ID 33
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
Alix|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21136642    
Organism Mus musculus
Experiment description Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons?
Authors "Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J"
Journal name PROTEOMICS_CL
Publication year 2007
Sample Oligodendrocytes
Sample name Oligodendrocytes
Oli-neu
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
Immunoelectron Microscopy
25
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
26
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
27
Experiment ID 34
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
TSG101|CD63|MHCI|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19190083    
Organism Homo sapiens
Experiment description Characterization of exosome-like vesicles released from human tracheobronchial ciliated epithelium: a possible role in innate defense.
Authors "Kesimer M, Scull M, Brighton B, Demaria G, Burns K, O'Neal W, Pickles RJ, Sheehan JK"
Journal name FASEB
Publication year 2009
Sample Tracheobronchial cells
Sample name Tracheobronchial epithelial cells
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density 1.16 -1.18 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study Mass spectrometry [QTOF]
Western blotting
Immunoelectron Microscopy
FACS
 Protein-protein interactions for Actb
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Rps3 27050
Co-fractionation Mus musculus
2 ABCG2 9429
Affinity Capture-MS Homo sapiens
3 MVP 9961
Affinity Capture-MS Homo sapiens
4 SRGAP1 57522
Affinity Capture-MS Homo sapiens
5 ACTR2 10097
Affinity Capture-MS Homo sapiens
6 ARHGAP21 57584
Affinity Capture-MS Homo sapiens
7 ABLIM1 3983
Affinity Capture-MS Homo sapiens
8 AP2B1 163
Affinity Capture-MS Homo sapiens
9 Pknox1  
Affinity Capture-MS Mus musculus
10 IMMT 10989
Affinity Capture-MS Homo sapiens
11 CCT3 7203
Affinity Capture-MS Homo sapiens
12 RAB1A 5861
Affinity Capture-MS Homo sapiens
13 AAK1 22848
Affinity Capture-MS Homo sapiens
14 VAPA 9218
Affinity Capture-MS Homo sapiens
15 Inadl  
Affinity Capture-MS Mus musculus
16 CPM 1368
Affinity Capture-MS Homo sapiens
17 ARPC2 10109
Affinity Capture-MS Homo sapiens
18 KCTD10 83892
Affinity Capture-MS Homo sapiens
19 CLTA 1211
Affinity Capture-MS Homo sapiens
20 Cotl1 72042
Two-hybrid Mus musculus
Reconstituted Complex Mus musculus
21 TPM2 7169
Affinity Capture-MS Homo sapiens
22 CAP1 10487
Affinity Capture-MS Homo sapiens
23 Ybx1 22608
Co-fractionation Mus musculus
24 AFAP1 60312
Affinity Capture-MS Homo sapiens
25 DBN1 1627
Affinity Capture-MS Homo sapiens
26 LIMA1 51474
Affinity Capture-MS Homo sapiens
27 EPS15 2060
Affinity Capture-MS Homo sapiens
28 INF2 64423
Affinity Capture-MS Homo sapiens
29 LGALS1 3956
Affinity Capture-MS Homo sapiens
30 SRGAP2 23380
Affinity Capture-MS Homo sapiens
31 CCT4 10575
Affinity Capture-MS Homo sapiens
32 BASP1 10409
Affinity Capture-MS Homo sapiens
33 Rps6 20104
Co-fractionation Mus musculus
34 Mks1  
Affinity Capture-MS Mus musculus
35 RAB14 51552
Affinity Capture-MS Homo sapiens
36 CFL1 1072
Two-hybrid Homo sapiens
37 CLTB 1212
Affinity Capture-MS Homo sapiens
38 RPL22 6146
Affinity Capture-MS Homo sapiens
39 Tmem173  
Proximity Label-MS Mus musculus
40 CAPZB 832
Affinity Capture-MS Homo sapiens
41 Cyfip1 20430
Affinity Capture-MS Mus musculus
42 PPP1R12A 4659
Affinity Capture-MS Homo sapiens
43 Ywhab 54401
Co-fractionation Mus musculus
44 COL5A1 1289
Affinity Capture-MS Homo sapiens
45 MYO5B 4645
Affinity Capture-MS Homo sapiens
46 DCTN4 51164
Affinity Capture-MS Homo sapiens
47 ACTR3 10096
Affinity Capture-MS Homo sapiens
48 AP2A1 160
Affinity Capture-MS Homo sapiens
49 TRMT112 51504
Affinity Capture-MS Homo sapiens
50 Invs  
Affinity Capture-MS Mus musculus
51 PPP1R18 170954
Affinity Capture-MS Homo sapiens
52 Rpl26 19941
Co-fractionation Mus musculus
53 STOM 2040
Affinity Capture-MS Homo sapiens
54 CD109 135228
Affinity Capture-MS Homo sapiens
55 ERC1 23085
Affinity Capture-MS Homo sapiens
56 SPECC1L 23384
Affinity Capture-MS Homo sapiens
57 Tagln2 21346
Co-fractionation Mus musculus
58 CHCHD3 54927