Gene ontology annotations for Actb
Experiment description of studies that identified Actb in exosomes
1
Experiment ID
26
ISEV standards
✔
EM
EV Biophysical techniques
✔
Alix|GAPDH|HSP70
EV Cytosolic markers
✔
CD9
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
19109410
Organism
Mus musculus
Experiment description
Proteomic and immunologic analyses of brain tumor exosomes.
Authors
Graner MW, Alzate O, Dechkovskaia AM, Keene JD, Sampson JH, Mitchell DA, Bigner DD
Journal name
FASEB
Publication year
2008
Sample
Brain cancer cells
Sample name
SMA560vIII
Isolation/purification methods
Differential centrifugation Sucrose density gradient
Flotation density
1.13-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [MALDI TOF/TOF] Western blotting
2
Experiment ID
3
ISEV standards
✔
IEM
EV Biophysical techniques
✔
HSP84|HSC73
EV Cytosolic markers
✔
MHCII|CD9|mac-1
EV Membrane markers
✔
CANX|HSP90B1
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
10545503
Organism
Mus musculus
Experiment description
Molecular characterization of dendritic cell-derived exosomes. Selective accumulation of the heat shock protein hsc73.
Authors
Thery C, Regnault A, Garin J, Wolfers J, Zitvogel L, Ricciardi-Castagnoli P, Raposo G, Amigorena S.
Journal name
JCB
Publication year
1999
Sample
Dendritic cells
Sample name
D1
Isolation/purification methods
Differential centrifugation Sucrose density gradient
Flotation density
1.14-1.20 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [MALDI TOF] Western blotting
3
Experiment ID
73
ISEV standards
✔
EM
EV Biophysical techniques
✔
HSP70|HSP90
EV Cytosolic markers
✘
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
18494037
Organism
Mus musculus
Experiment description
Difference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes.
Authors
Ji H, Erfani N, Tauro BJ, Kapp EA, Zhu HJ, Moritz RL, Lim JW, Simpson RJ
Journal name
ELEC
Publication year
2008
Sample
Embryonic fibroblasts
Sample name
NIH3T3
Isolation/purification methods
Differential centrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [LCQ DECA] Western blotting
4
Experiment ID
210
ISEV standards
✔
EM
EV Biophysical techniques
✘
EV Cytosolic markers
✔
CD81|FLOT1
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23260141
Organism
Mus musculus
Experiment description
Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors
Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL.
Journal name
Cell
Publication year
2012
Sample
Fibroblasts
Sample name
Normal-Fibroblasts (L cells)
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
5
Experiment ID
214
ISEV standards
✔
EM
EV Biophysical techniques
✘
EV Cytosolic markers
✘
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23658846
Organism
Mus musculus
Experiment description
Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors
Hassani K, Olivier M.
Journal name
PLoS Negl Trop Dis
Publication year
2013
Sample
Macrophages
Sample name
Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Filtration Protease inhibitors Sucrose density gradient Filtration Ultracentrifugation
Flotation density
1.13-1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
6
Experiment ID
215
ISEV standards
✔
EM
EV Biophysical techniques
✘
EV Cytosolic markers
✘
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23658846
Organism
Mus musculus
Experiment description
Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors
Hassani K, Olivier M.
Journal name
PLoS Negl Trop Dis
Publication year
2013
Sample
Macrophages
Sample name
LPS-treated-Macrophage (J774A.1)
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Filtration Protease inhibitors Sucrose density gradient Filtration Ultracentrifugation
Flotation density
1.13-1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
7
Experiment ID
216
ISEV standards
✔
EM
EV Biophysical techniques
✘
EV Cytosolic markers
✘
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23658846
Organism
Mus musculus
Experiment description
Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors
Hassani K, Olivier M.
Journal name
PLoS Negl Trop Dis
Publication year
2013
Sample
Macrophages
Sample name
Normal-Macrophage (J774A.1)
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Filtration Protease inhibitors Sucrose density gradient Filtration Ultracentrifugation
Flotation density
1.13-1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
8
Experiment ID
5
ISEV standards
✔
EM|IEM
EV Biophysical techniques
✘
EV Cytosolic markers
✔
CD86|CD80
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
11145662
Organism
Mus musculus
Experiment description
Mast cell-dependent B and T lymphocyte activation is mediated by the secretion of immunologically active exosomes.
Authors
Skokos D, Le Panse S, Villa I, Rousselle JC, Peronet R, David B, Namane A, Mecheri S
Journal name
JIMMU
Publication year
2001
Sample
Mast cells
Sample name
P815 MC9 Bone marrow-derived mast cells
Isolation/purification methods
Differential centrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [MALDI TOF] ELISA
9
Experiment ID
15
ISEV standards
✔
EM
EV Biophysical techniques
✘
EV Cytosolic markers
✔
CD63
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
17486113
Organism
Mus musculus Homo sapiens
Experiment description
Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors
Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name
NCB
Publication year
2007
Sample
Mast cells
Sample name
MC9 Bone marrow-derived mast cells HMC-1
Isolation/purification methods
Filtration Ultracentrifugation Sucrose density gradient
Flotation density
1.11-1.21 g/mL
Molecules identified in the study
Protein mRNA miRNA
Methods used in the study
Mass spectrometry [MALDI TOF] Western blotting Microarray miRCURY LNA Array
10
Experiment ID
15
ISEV standards
✔
EM
EV Biophysical techniques
✘
EV Cytosolic markers
✔
CD63
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
mrna
Identification method
Microarray
PubMed ID
17486113
Organism
Mus musculus Homo sapiens
Experiment description
Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors
Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name
NCB
Publication year
2007
Sample
Mast cells
Sample name
MC9 Bone marrow-derived mast cells HMC-1
Isolation/purification methods
Filtration Ultracentrifugation Sucrose density gradient
Flotation density
1.11-1.21 g/mL
Molecules identified in the study
Protein mRNA miRNA
Methods used in the study
Mass spectrometry [MALDI TOF] Western blotting Microarray miRCURY LNA Array
11
Experiment ID
39
ISEV standards
✔
IEM
EV Biophysical techniques
✔
TSG101|HSC70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
15210972
Organism
Mus musculus
Experiment description
Cells release prions in association with exosomes.
Authors
Fevrier B, Vilette D, Archer F, Loew D, Faigle W, Vidal M, Laude H, Raposo G
Journal name
PNAS
Publication year
2004
Sample
Mov neuroglial cells
Sample name
Mov neuroglial cell
Isolation/purification methods
Differential centrifugation Sucrose density gradient
Flotation density
1.14 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [QSTAR] Western blotting Immunoelectron Microscopy
12
Experiment ID
262
ISEV standards
✔
EM
EV Biophysical techniques
✔
Alix|TSG101|HSP70|HSP90
EV Cytosolic markers
✔
CD63|CD9|
EV Membrane markers
✘
EV Negative markers
✔
Y
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
25242146
Organism
Mus musculus
Experiment description
Extracellular Vesicles from Neural Stem Cells Transfer IFN-γ via Ifngr1 to Activate Stat1 Signaling in Target Cells
Authors
Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino
Journal name
Molecular Cell
Publication year
2014
Sample
Neural stem cells
Sample name
NPCs
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
1.13-1.20 g/mL
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry Microarray
13
Experiment ID
263
ISEV standards
✘
EV Biophysical techniques
✘
EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
25242146
Organism
Mus musculus
Experiment description
Extracellular Vesicles from Neural Stem Cells Transfer IFN-γ via Ifngr1 to Activate Stat1 Signaling in Target Cells
Authors
Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino
Journal name
Molecular Cell
Publication year
2014
Sample
Neural stem cells
Sample name
NPCs - Th1 treated
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
1.13-1.20 g/mL
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
14
Experiment ID
264
ISEV standards
✘
EV Biophysical techniques
✘
EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
25242146
Organism
Mus musculus
Experiment description
Extracellular Vesicles from Neural Stem Cells Transfer IFN-γ via Ifngr1 to Activate Stat1 Signaling in Target Cells
Authors
Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino
Journal name
Molecular Cell
Publication year
2014
Sample
Neural stem cells
Sample name
NPCs - Th2 treated
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
1.13-1.20 g/mL
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
15
Experiment ID
33
ISEV standards
✔
EM|IEM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✘
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
21136642
Organism
Mus musculus
Experiment description
Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons?
Authors
Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J
Journal name
PROTEOMICS_CL
Publication year
2007
Sample
Oligodendrocytes
Sample name
Oligodendrocytes Oli-neu
Isolation/purification methods
Differential centrifugation Sucrose density gradient
Flotation density
1.10-1.14 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [QTOF] Western blotting Immunoelectron Microscopy
16
Experiment ID
188
ISEV standards
✔
EM
EV Biophysical techniques
✔
GAPDH|UCHL1|HSP90
EV Cytosolic markers
✘
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
19351151
Organism
Mus musculus
Experiment description
Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors
Lee HS, Jeong J, Lee KJ.
Journal name
J Proteome Res
Publication year
2009
Sample
Pancreatic cells
Sample name
Pancreatic beta cell (NIT-1)
Isolation/purification methods
Differential centrifugation Sucrose density gradient
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
17
Experiment ID
34
ISEV standards
✔
EM|IEM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD63|MHCI|MHCII
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
19190083
Organism
Homo sapiens
Experiment description
Characterization of exosome-like vesicles released from human tracheobronchial ciliated epithelium: a possible role in innate defense.
Authors
Kesimer M, Scull M, Brighton B, Demaria G, Burns K, O'Neal W, Pickles RJ, Sheehan JK
Journal name
FASEB
Publication year
2009
Sample
Tracheobronchial cells
Sample name
Tracheobronchial epithelial cells
Isolation/purification methods
Differential centrifugation Filtration Sucrose density gradient
Flotation density
1.16 -1.18 g/mL
Molecules identified in the study
Protein mRNA
Methods used in the study
Mass spectrometry [QTOF] Western blotting Immunoelectron Microscopy FACS
Protein-protein interactions for Actb
Protein Interactor
ExoCarta ID
Identification method
PubMed
Species
1
CPNE2
221184
Two-hybrid
Homo sapiens
2
CPNE4
131034
Two-hybrid
Homo sapiens
Reconstituted Complex
Homo sapiens
3
CPNE1
8904
Two-hybrid
Homo sapiens
Reconstituted Complex
Homo sapiens
4
Pknox1
Affinity Capture-MS
Mus musculus
View the network
image/svg+xml
Pathways in which Actb is involved