Gene description for EDC3
Gene name enhancer of mRNA decapping 3
Gene symbol EDC3
Other names/aliases LSM16
YJDC
YJEFN2
Species Homo sapiens
 Database cross references - EDC3
ExoCarta ExoCarta_80153
Vesiclepedia VP_80153
Entrez Gene 80153
HGNC 26114
MIM 609842
UniProt Q96F86  
 EDC3 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for EDC3
Molecular Function
    mRNA binding GO:0003729 IBA
    protein binding GO:0005515 IPI
    identical protein binding GO:0042802 IPI
Biological Process
    deadenylation-independent decapping of nuclear-transcribed mRNA GO:0031087 IBA
    P-body assembly GO:0033962 IBA
Subcellular Localization
    P-body GO:0000932 IBA
    cytosol GO:0005829 TAS
    membrane GO:0016020 HDA
    cytoplasmic ribonucleoprotein granule GO:0036464 IDA
 Experiment description of studies that identified EDC3 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for EDC3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 Edc4  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
3 QARS 5859
Two-hybrid Homo sapiens
4 KSR1  
Affinity Capture-MS Homo sapiens
5 FAM167A  
Affinity Capture-MS Homo sapiens
6 DCP1B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 PIM1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 CARD9 64170
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 ITGB8 3696
Affinity Capture-MS Homo sapiens
10 CDC25B 994
Affinity Capture-MS Homo sapiens
11 SRGAP2 23380
Affinity Capture-MS Homo sapiens
12 CBY1  
Affinity Capture-MS Homo sapiens
13 CLTB 1212
Affinity Capture-MS Homo sapiens
14 CCDC102B  
Affinity Capture-MS Homo sapiens
15 KIF13B 23303
Affinity Capture-MS Homo sapiens
16 ZBTB21  
Affinity Capture-MS Homo sapiens
17 ATXN2L 11273
Affinity Capture-MS Homo sapiens
18 ZNF638 27332
Affinity Capture-MS Homo sapiens
19 RAB3IP  
Affinity Capture-MS Homo sapiens
20 PPP1R35  
Affinity Capture-MS Homo sapiens
21 YWHAG 7532
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 YWHAH 7533
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 DDX6 1656
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 LRFN1  
Affinity Capture-MS Homo sapiens
25 PABPC4 8761
Affinity Capture-MS Homo sapiens
26 LSM12 124801
Affinity Capture-MS Homo sapiens
27 YWHAB 7529
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 CPEB1  
Proximity Label-MS Homo sapiens
29 AGAP1  
Affinity Capture-MS Homo sapiens
30 EDC4 23644
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 CDK16 5127
Affinity Capture-MS Homo sapiens
32 PARK2  
Affinity Capture-MS Homo sapiens
33 SIPA1L1 26037
Affinity Capture-MS Homo sapiens
34 TESK2  
Affinity Capture-MS Homo sapiens
35 FAM96B 51647
Affinity Capture-MS Homo sapiens
36 CLTCL1 8218
Affinity Capture-MS Homo sapiens
37 TRIM37  
Proximity Label-MS Homo sapiens
38 FBXO16  
Affinity Capture-MS Homo sapiens
39 PABPC1 26986
Affinity Capture-MS Homo sapiens
40 RCCD1  
Affinity Capture-MS Homo sapiens
41 YWHAE 7531
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 IGHG1 3500
Affinity Capture-MS Homo sapiens
43 SLX4  
Affinity Capture-MS Homo sapiens
44 CRKL 1399
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 IGHG4 3503
Affinity Capture-MS Homo sapiens
46 DCP1A 55802
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 WIPI1 55062
Affinity Capture-MS Homo sapiens
48 PNMA2  
Affinity Capture-MS Homo sapiens
49 YWHAQ 10971
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 AQP12B  
Affinity Capture-MS Homo sapiens
51 KCTD3  
Affinity Capture-MS Homo sapiens
52 ARL4C 10123
Affinity Capture-MS Homo sapiens
53 OGT 8473
Reconstituted Complex Homo sapiens
54 CCDC59  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 LIMA1 51474
Affinity Capture-MS Homo sapiens
56 NTRK1 4914
Affinity Capture-MS Homo sapiens
57 USP4 7375
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 DCLK1 9201
Affinity Capture-MS Homo sapiens
59 XPO1 7514
Affinity Capture-MS Homo sapiens
60 CRYAA 1409
Affinity Capture-MS Homo sapiens
61 CUL3 8452
Affinity Capture-MS Homo sapiens
62 YWHAZ 7534
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 LOC254896  
Affinity Capture-MS Homo sapiens
64 ANXA10 11199
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
65 GADD45GIP1  
Affinity Capture-MS Homo sapiens
66 PNLIPRP2  
Affinity Capture-MS Homo sapiens
67 SYDE1 85360
Affinity Capture-MS Homo sapiens
68 RPA3 6119
Proximity Label-MS Homo sapiens
69 DYNC1LI1 51143
Proximity Label-MS Homo sapiens
70 GIGYF1  
Affinity Capture-MS Homo sapiens
71 FAM153A  
Affinity Capture-MS Homo sapiens
72 GOLGA1  
Proximity Label-MS Homo sapiens
73 PNRC2  
Affinity Capture-MS Homo sapiens
74 CLTC 1213
Affinity Capture-MS Homo sapiens
75 EIF4E2  
Affinity Capture-MS Homo sapiens
76 BAG4  
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
77 EIF3H 8667
Affinity Capture-MS Homo sapiens
78 MRPL42  
Affinity Capture-MS Homo sapiens
79 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
80 FASN 2194
Negative Genetic Homo sapiens
81 GRN 2896
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
82 STK16 8576
Affinity Capture-MS Homo sapiens
83 TERF2  
Affinity Capture-MS Homo sapiens
84 EFHC2  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
85 ZFP36  
Affinity Capture-Western Homo sapiens
86 DENND1A 57706
Affinity Capture-MS Homo sapiens
87 KANK1  
Affinity Capture-MS Homo sapiens
88 LSM14A 26065
Affinity Capture-MS Homo sapiens
89 SRSF12  
Affinity Capture-MS Homo sapiens
90 HNRNPA2B1 3181
Affinity Capture-MS Homo sapiens
91 ATXN2 6311
Affinity Capture-MS Homo sapiens
92 SQSTM1 8878
Proximity Label-MS Homo sapiens
93 MAGI1  
Affinity Capture-MS Homo sapiens
94 ARHGAP18 93663
Affinity Capture-MS Homo sapiens
95 ALDH18A1 5832
Affinity Capture-MS Homo sapiens
96 EDC3 80153
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
97 LPXN 9404
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
98 C10orf88  
Affinity Capture-MS Homo sapiens
99 PSMA5 5686
Affinity Capture-MS Homo sapiens
100 KLK10 5655
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
101 UPF1 5976
Affinity Capture-Western Homo sapiens
102 CLTA 1211
Affinity Capture-MS Homo sapiens
103 KANK2 25959
Affinity Capture-MS Homo sapiens
104 SERBP1 26135
Affinity Capture-MS Homo sapiens
105 DCP2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
106 EP300 2033
Affinity Capture-MS Homo sapiens
107 SH3PXD2A 9644
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which EDC3 is involved
PathwayEvidenceSource
Deadenylation-dependent mRNA decay TAS Reactome
Metabolism of RNA TAS Reactome
mRNA decay by 5' to 3' exoribonuclease TAS Reactome





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