Gene description for DAPK3
Gene name death-associated protein kinase 3
Gene symbol DAPK3
Other names/aliases DLK
ZIP
ZIPK
Species Homo sapiens
 Database cross references - DAPK3
ExoCarta ExoCarta_1613
Vesiclepedia VP_1613
Entrez Gene 1613
HGNC 2676
MIM 603289
UniProt O43293  
 DAPK3 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for DAPK3
Molecular Function
    protein kinase activity GO:0004672 IDA
    protein serine/threonine kinase activity GO:0004674 IBA
    protein serine/threonine kinase activity GO:0004674 IDA
    protein serine/threonine kinase activity GO:0004674 TAS
    3-phosphoinositide-dependent protein kinase activity GO:0004676 IEA
    DNA-dependent protein kinase activity GO:0004677 IEA
    AMP-activated protein kinase activity GO:0004679 IEA
    eukaryotic translation initiation factor 2alpha kinase activity GO:0004694 IEA
    ribosomal protein S6 kinase activity GO:0004711 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IDA
    cAMP response element binding protein binding GO:0008140 TAS
    small GTPase binding GO:0031267 IDA
    histone H3S10 kinase activity GO:0035175 IEA
    histone H3T11 kinase activity GO:0035402 IEA
    histone H3T6 kinase activity GO:0035403 IEA
    histone H2AXS139 kinase activity GO:0035979 IEA
    identical protein binding GO:0042802 IPI
    protein homodimerization activity GO:0042803 IDA
    leucine zipper domain binding GO:0043522 IPI
    histone H3S28 kinase activity GO:0044022 IEA
    histone H4S1 kinase activity GO:0044023 IEA
    histone H2AS1 kinase activity GO:0044024 IEA
    histone H2BS14 kinase activity GO:0044025 IEA
    histone H3T3 kinase activity GO:0072354 IEA
    histone H2AS121 kinase activity GO:0072371 IEA
    Rho-dependent protein serine/threonine kinase activity GO:0072518 IEA
    protein serine kinase activity GO:0106310 IEA
    histone H2BS36 kinase activity GO:0140823 IEA
    histone H3S57 kinase activity GO:0140855 IEA
    histone H3T45 kinase activity GO:0140857 IEA
    histone H2AT120 kinase activity GO:1990244 IEA
Biological Process
    chromatin remodeling GO:0006338 IEA
    regulation of DNA-templated transcription GO:0006355 TAS
    protein phosphorylation GO:0006468 IDA
    apoptotic process GO:0006915 IDA
    apoptotic process GO:0006915 IMP
    regulation of smooth muscle contraction GO:0006940 TAS
    regulation of mitotic nuclear division GO:0007088 TAS
    regulation of mitotic cell cycle GO:0007346 IMP
    regulation of cell shape GO:0008360 IEA
    regulation of autophagy GO:0010506 TAS
    negative regulation of translation GO:0017148 IDA
    positive regulation of cell migration GO:0030335 IEA
    regulation of actin cytoskeleton organization GO:0032956 IDA
    regulation of actin cytoskeleton organization GO:0032956 TAS
    intracellular signal transduction GO:0035556 IBA
    intracellular signal transduction GO:0035556 IDA
    regulation of apoptotic process GO:0042981 TAS
    positive regulation of apoptotic process GO:0043065 IBA
    positive regulation of apoptotic process GO:0043065 IDA
    regulation of myosin II filament organization GO:0043519 IEA
    protein autophosphorylation GO:0046777 IDA
    protein autophosphorylation GO:0046777 TAS
    regulation of focal adhesion assembly GO:0051893 IDA
    cellular response to type II interferon GO:0071346 IDA
    positive regulation of canonical Wnt signaling pathway GO:0090263 IMP
    apoptotic signaling pathway GO:0097190 IEA
    regulation of cell motility GO:2000145 TAS
Subcellular Localization
    chromosome, centromeric region GO:0000775 IDA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 ISS
    nucleoplasm GO:0005654 IDA
    cytoplasm GO:0005737 IBA
    spindle GO:0005819 IDA
    cytosol GO:0005829 IDA
    PML body GO:0016605 IEA
    midbody GO:0030496 IDA
 Experiment description of studies that identified DAPK3 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for DAPK3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CDC14A  
Proximity Label-MS Homo sapiens
2 LIMA1 51474
Affinity Capture-MS Homo sapiens
3 UBE3A 7337
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
4 RAD21 5885
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
5 PPP1CB 5500
Affinity Capture-MS Homo sapiens
6 Itga5 16402
Affinity Capture-MS Mus musculus
7 KIF14 9928
Affinity Capture-MS Homo sapiens
8 CEP170P1  
Affinity Capture-MS Homo sapiens
9 AATF  
Co-localization Homo sapiens
10 Cd2ap 12488
Affinity Capture-MS Mus musculus
11 Myh9 17886
Affinity Capture-MS Mus musculus
12 Tpm1 22003
Affinity Capture-MS Mus musculus
13 TNFRSF8 943
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 PAWR 5074
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
15 C15orf59  
Affinity Capture-MS Homo sapiens
16 CEP170 9859
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 KLHDC3  
Affinity Capture-MS Homo sapiens
18 ANXA1 301
Protein-peptide Homo sapiens
19 Myo1c 17913
Affinity Capture-MS Mus musculus
20 MYO18A 399687
Affinity Capture-MS Homo sapiens
21 TCP10L  
Two-hybrid Homo sapiens
22 PRKCZ 5590
Reconstituted Complex Homo sapiens
23 TSG101 7251
Co-localization Homo sapiens
24 GRIN3B  
Affinity Capture-MS Homo sapiens
25 CAPZA2 830
Affinity Capture-MS Homo sapiens
26 TP53 7157
Biochemical Activity Homo sapiens
27 CHEK2  
Biochemical Activity Homo sapiens
28 MYO5C 55930
Affinity Capture-MS Homo sapiens
29 LUZP1 7798
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 CAPZB 832
Affinity Capture-MS Homo sapiens
31 HSP90AA1 3320
Affinity Capture-Luminescence Homo sapiens
32 Actb 11461
Affinity Capture-MS Mus musculus
33 RMDN3 55177
Proximity Label-MS Homo sapiens
34 MYO19  
Affinity Capture-MS Homo sapiens
35 RPL34 6164
Two-hybrid Homo sapiens
36 Myh10 77579
Affinity Capture-MS Mus musculus
37 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
38 OGT 8473
Reconstituted Complex Homo sapiens
39 APEX1 328
Affinity Capture-RNA Homo sapiens
40 CDK2 1017
Affinity Capture-MS Homo sapiens
41 PARK2  
Affinity Capture-MS Homo sapiens
42 SYNPO 11346
Affinity Capture-MS Homo sapiens
43 FILIP1  
Affinity Capture-MS Homo sapiens
44 MEAF6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 Flnb 286940
Affinity Capture-MS Mus musculus
46 Dctn3  
Affinity Capture-MS Mus musculus
47 MYH9 4627
Affinity Capture-MS Homo sapiens
48 DAPK3 1613
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
49 FLNA 2316
Affinity Capture-MS Homo sapiens
50 WHAMMP3  
Affinity Capture-MS Homo sapiens
51 Atf4  
Two-hybrid Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
52 CIT 11113
Affinity Capture-MS Homo sapiens
53 CALD1 800
Affinity Capture-MS Homo sapiens
54 CDKN1A  
Protein-peptide Homo sapiens
Biochemical Activity Homo sapiens
55 GRB14 2888
Reconstituted Complex Homo sapiens
56 AK3 50808
Two-hybrid Homo sapiens
57 IQGAP1 8826
Affinity Capture-MS Homo sapiens
58 UBE2D3 7323
Affinity Capture-Western Homo sapiens
59 Coro1c 23790
Affinity Capture-MS Mus musculus
60 Mad2l1 56150
Affinity Capture-MS Mus musculus
61 ACTC1 70
Proximity Label-MS Homo sapiens
62 Flot2 14252
Affinity Capture-MS Mus musculus
63 ULK1  
Affinity Capture-Western Homo sapiens
64 KIF2B 84643
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 MDM2  
Protein-peptide Homo sapiens
Biochemical Activity Homo sapiens
Co-localization Homo sapiens
66 NEDD4L 23327
Biochemical Activity Homo sapiens
67 DAXX  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
68 AR 367
Co-localization Homo sapiens
Co-localization Homo sapiens
69 GPS2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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