Gene description for PSMC2
Gene name proteasome (prosome, macropain) 26S subunit, ATPase, 2
Gene symbol PSMC2
Other names/aliases MSS1
Nbla10058
S7
Species Homo sapiens
 Database cross references - PSMC2
ExoCarta ExoCarta_5701
Vesiclepedia VP_5701
Entrez Gene 5701
HGNC 9548
MIM 154365
UniProt P35998  
 PSMC2 identified in sEVs derived from the following tissue/cell type
Bladder cancer cells 20224111    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Chondrocytes 35931686    
Chondrocytes 35931686    
Chondrocytes 35931686    
Chondrocytes 35931686    
Colorectal cancer cells 34887515    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 36706192    
Embryonic kidney cells 36706192    
Foreskin fibroblasts 34108659    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Lymphoma cells 34108659    
Mammary cancer-associated fibroblasts 34108659    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Mesenchymal stem cells 36408942    
Monocytic leukemia cells 34108659    
Neuroblastoma cells 25944692    
Normal mammary epithelial cells 34108659    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic duct epithalial cells 34108659    
Pancreatic duct epithalial cells 34108659    
Pluripotent stem cells 34108659    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
T lymphocytes 34108659    
Thymus 23844026    
 Gene ontology annotations for PSMC2
Molecular Function
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ATP hydrolysis activity GO:0016887 IEA
    proteasome-activating activity GO:0036402 IBA
    proteasome-activating activity GO:0036402 IDA
    proteasome-activating activity GO:0036402 TAS
Biological Process
    osteoblast differentiation GO:0001649 HDA
    ubiquitin-dependent protein catabolic process GO:0006511 IDA
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IBA
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 NAS
    positive regulation of proteasomal protein catabolic process GO:1901800 IC
Subcellular Localization
    proteasome complex GO:0000502 IDA
    proteasome complex GO:0000502 NAS
    P-body GO:0000932 ISS
    extracellular region GO:0005576 TAS
    nucleus GO:0005634 HDA
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 TAS
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    proteasome regulatory particle, base subcomplex GO:0008540 IBA
    membrane GO:0016020 HDA
    proteasome accessory complex GO:0022624 ISS
    secretory granule lumen GO:0034774 TAS
    cytoplasmic ribonucleoprotein granule GO:0036464 IDA
    ficolin-1-rich granule lumen GO:1904813 TAS
 Experiment description of studies that identified PSMC2 in sEVs
1
Experiment ID 76
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|GAPDH|HSP90|CD81|CD9|CD63|LAMP1|MHCI
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20224111    
Organism Homo sapiens
Experiment description Proteomics analysis of bladder cancer exosomes.
Authors "Welton JL, Khanna S, Giles PJ, Brennan P, Brewis IA, Staffurth J, Mason MD, Clayton A."
Journal name MCP
Publication year 2010
Sample Bladder cancer cells
Sample name HT1376
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF/TOF]
Western blotting
FACS
2
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 412
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MCF7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
7
Experiment ID 414
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
8
Experiment ID 426
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 427
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 494
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Osteoarthritic cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
11
Experiment ID 496
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Healthy cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
12
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
13
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
14
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
15
Experiment ID 407
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|TSG101|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
16
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
17
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
18
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
19
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
20
Experiment ID 1275
MISEV standards
Biophysical techniques
✔
CD9|TSG101|FLOT1|FLOT2|RAB35|RAB5A|GAPDH|TFRC|CD63|Alix
Enriched markers
✔
PCNA|CANX|GAPDH|HSP90AA1|HSP90B1
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36706192    
Organism Homo sapiens
Experiment description Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles
Authors "Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q."
Journal name Sci Adv
Publication year 2023
Sample Embryonic kidney cells
Sample name HEK293T
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
21
Experiment ID 1280
MISEV standards
Biophysical techniques
✔
TSG101|CD9|FLOT1|FLOT2|RAB35|RAB5A|GAPDH|TFRC|CD63|Alix
Enriched markers
✔
PCNA|CANX|GAPDH|HSP90AA1|HSP90B1
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36706192    
Organism Homo sapiens
Experiment description Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles
Authors "Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q."
Journal name Sci Adv
Publication year 2023
Sample Embryonic kidney cells
Sample name HEK293T - Fraction 5
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density 1.151 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
22
Experiment ID 405
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Foreskin fibroblasts
Sample name BJ
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
23
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
24
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
25
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
26
Experiment ID 417
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Lymphoma cells
Sample name Raji
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
27
Experiment ID 411
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Mammary cancer-associated fibroblasts
Sample name mCAF
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
28
Experiment ID 254
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name MNT-1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
29
Experiment ID 255
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name G1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
30
Experiment ID 256
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 501mel
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
31
Experiment ID 257
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name Daju
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
32
Experiment ID 259
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name A375M
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
33
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
34
Experiment ID 418
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Monocytic leukemia cells
Sample name THP-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
35
Experiment ID 418
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Monocytic leukemia cells
Sample name THP-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
36
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
37
Experiment ID 413
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Normal mammary epithelial cells
Sample name MCF10A
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
38
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
39
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
40
Experiment ID 406
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35|CD81
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name BxPC3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
41
Experiment ID 415
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
42
Experiment ID 434
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
43
Experiment ID 435
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
44
Experiment ID 408
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic duct epithalial cells
Sample name HPDE
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
45
Experiment ID 409
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic duct epithalial cells
Sample name HPNE
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
46
Experiment ID 416
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pluripotent stem cells
Sample name PSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
47
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
48
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
49
Experiment ID 410
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample T lymphocytes
Sample name Jurkat
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
50
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PSMC2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CNOT7 29883
Two-hybrid Homo sapiens
2 PSMA6 5687
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
3 LDHA 3939
Co-fractionation Homo sapiens
4 UBE2H 7328
Affinity Capture-MS Homo sapiens
5 SMC3 9126
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 GFPT1 2673
Co-fractionation Homo sapiens
7 Mdm2  
Affinity Capture-MS Mus musculus
8 CHMP5 51510
Co-fractionation Homo sapiens
9 SPRTN  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 MTCH1 23787
Co-fractionation Homo sapiens
11 PCK1 5105
Affinity Capture-MS Homo sapiens
12 NEURL4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 Psmb5 19173
Affinity Capture-MS Mus musculus
14 PSMD6 9861
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
15 ZCCHC8 55596
Co-fractionation Homo sapiens
16 LDHB 3945
Co-fractionation Homo sapiens
17 Rad23a  
Affinity Capture-Western Rattus norvegicus
18 PSMA4 5685
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
19 RIT1 6016
Negative Genetic Homo sapiens
20 PSMD11 5717
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
21 SEPT10 151011
Proximity Label-MS Homo sapiens
22 DNAJA4 55466
Co-fractionation Homo sapiens
23 EBNA-LP  
Affinity Capture-MS
24 PSMB8 5696
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
25 GTF2F1 2962
Affinity Capture-Western Homo sapiens
26 UBE2C 11065
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
27 CCT3 7203
Co-fractionation Homo sapiens
28 UBE3A 7337
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 UBC 7316
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
30 CCT4 10575
Co-fractionation Homo sapiens
31 CCT6B 10693
Co-fractionation Homo sapiens
32 LOC100132735  
Protein-RNA Homo sapiens
33 PSMD10 5716
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 DERL1 79139
Affinity Capture-MS Homo sapiens
35 RPS6KB2  
Affinity Capture-MS Homo sapiens
36 SOX2  
Affinity Capture-MS Homo sapiens
37 ATG4C  
Affinity Capture-MS Homo sapiens
38 HSPA5 3309
Co-fractionation Homo sapiens
39 SLC4A1AP  
Co-fractionation Homo sapiens
40 LRPPRC 10128
Co-fractionation Homo sapiens
41 KIAA1429 25962
Affinity Capture-MS Homo sapiens
42 RNH1 6050
Co-fractionation Homo sapiens
43 MAP3K5 4217
Affinity Capture-Western Homo sapiens
44 PAAF1 80227
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
45 VTA1 51534
Co-fractionation Homo sapiens
46 PCDH10 57575
Affinity Capture-Western Homo sapiens
47 CCDC92  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 B3GNT2 10678
Affinity Capture-MS Homo sapiens
49 TUBB 203068
Co-fractionation Homo sapiens
50 KLF8  
Affinity Capture-MS Homo sapiens
51 NDRG1 10397
Affinity Capture-MS Homo sapiens
52 FAM98A 25940
Cross-Linking-MS (XL-MS) Homo sapiens
53 CCAR2 57805
Co-fractionation Homo sapiens
54 KIF5B 3799
Co-fractionation Homo sapiens
55 SPAG9 9043
Co-fractionation Homo sapiens
56 RPN1 6184
Cross-Linking-MS (XL-MS) Homo sapiens
Co-fractionation Homo sapiens
57 CLEC2B  
Affinity Capture-MS Homo sapiens
58 PSMD9 5715
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
59 C7orf76  
Affinity Capture-MS Homo sapiens
60 PSMD12 5718
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 HSPH1 10808
Co-fractionation Homo sapiens
62 PSMB6 5694
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
63 PSMC6 5706
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
64 PSMC2 5701
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
65 UBQLN1 29979
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
66 ABCE1 6059
Affinity Capture-MS Homo sapiens
67 KRAS 3845
Negative Genetic Homo sapiens
68 MID2 11043
Affinity Capture-MS Homo sapiens
69 HERC2 8924
Affinity Capture-MS Homo sapiens
70 GTF2B 2959
Affinity Capture-Western Homo sapiens
71 PPP2R1A 5518
Co-fractionation Homo sapiens
72 UCHL5 51377
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
73 UFC1 51506
Co-fractionation Homo sapiens
74 PSMB9 5698
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
75 PSMA8 143471
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
76 PMS2  
Affinity Capture-MS Homo sapiens
77 CDC73  
Affinity Capture-MS Homo sapiens
78 PSMD5 5711
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
79 SEC23A 10484
Co-fractionation Homo sapiens
80 POLR2M  
Two-hybrid Homo sapiens
81 SERBP1 26135
Affinity Capture-MS Homo sapiens
82 MIDN 90007
Affinity Capture-MS Homo sapiens
83 YAF2  
Affinity Capture-MS Homo sapiens
84 SOD1 6647
Affinity Capture-MS Homo sapiens
85 PSMC3 5702
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
86 RAD23A 5886
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
87 GSK3B 2932
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
88 KIF14 9928
Affinity Capture-MS Homo sapiens
89 OTUD1 220213
Affinity Capture-MS Homo sapiens
90 INSIG2  
Affinity Capture-MS Homo sapiens
91 POLA2  
Co-fractionation Homo sapiens
92 KLF16  
Affinity Capture-MS Homo sapiens
93 PPM1D  
Affinity Capture-MS Homo sapiens
94 XPO1 7514
Co-fractionation Homo sapiens
95 GOLGA4  
Co-fractionation Homo sapiens
96 KRT19 3880
Proximity Label-MS Homo sapiens
97 RAD23B 5887
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
98 SFSWAP  
Co-fractionation Homo sapiens
99 PSMB4 5692
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
100 CEP55 55165
Two-hybrid Homo sapiens
101 PRPH 5630
Proximity Label-MS Homo sapiens
102 USP14 9097
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
103 PSMD4 5710
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
104 TGM2 7052
Co-fractionation Homo sapiens
105 PARK2  
Reconstituted Complex Homo sapiens
106 KLC1 3831
Co-fractionation Homo sapiens
107 AMFR 267
Affinity Capture-MS Homo sapiens
108 PSME1 5720
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
109 PSMC1 5700
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
110 PSMD14 10213
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
111 PSMB1 5689
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
112 SNRNP200 23020
Co-fractionation Homo sapiens
113 ACACA 31
Co-fractionation Homo sapiens
114 TPM4 7171
Co-fractionation Homo sapiens
115 NCKAP1 10787
Co-fractionation Homo sapiens
116 MAPT  
Affinity Capture-Western Homo sapiens
117 SIRT7  
Affinity Capture-MS Homo sapiens
118 TNPO2 30000
Co-fractionation Homo sapiens
119 PSMD2 5708
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
120 MYH9 4627
Co-fractionation Homo sapiens
121 UBR5 51366
Co-fractionation Homo sapiens
122 NTRK1 4914
Affinity Capture-MS Homo sapiens
123 UBQLN2 29978
Reconstituted Complex Homo sapiens
124 ACADM 34
Co-fractionation Homo sapiens
125 DNAJA1 3301
Co-fractionation Homo sapiens
126 NOS2  
Affinity Capture-MS Homo sapiens
127 UBE2Q1 55585
Co-fractionation Homo sapiens
128 RPA4  
Proximity Label-MS Homo sapiens
129 CRY2  
Affinity Capture-MS Homo sapiens
130 SMC1A 8243
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
131 ARHGEF12 23365
Affinity Capture-MS Homo sapiens
132 GTF2H1 2965
Affinity Capture-Western Homo sapiens
133 TPM3 7170
Co-fractionation Homo sapiens
134 CCDC74B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
135 ATG7 10533
Co-fractionation Homo sapiens
136 Ksr1  
Affinity Capture-MS Mus musculus
137 CYLD  
Affinity Capture-MS Homo sapiens
138 SRPK1 6732
Co-fractionation Homo sapiens
139 UBLCP1  
Affinity Capture-MS Homo sapiens
140 OGT 8473
Reconstituted Complex Homo sapiens
141 VCP 7415
Co-fractionation Homo sapiens
142 UBR2 23304
Co-fractionation Homo sapiens
143 YWHAH 7533
Co-fractionation Homo sapiens
144 BAG3 9531
Affinity Capture-MS Homo sapiens
145 ANLN 54443
Affinity Capture-MS Homo sapiens
146 PSMA7 5688
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
147 PRIM1  
Co-fractionation Homo sapiens
148 VPS26A 9559
Co-fractionation Homo sapiens
149 TMPO 7112
Affinity Capture-MS Homo sapiens
150 POLR2C 5432
Proximity Label-MS Homo sapiens
151 PSMA3 5684
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
152 HIST1H3A 8350
Cross-Linking-MS (XL-MS) Homo sapiens
153 CLIP1 6249
Co-fractionation Homo sapiens
154 AIFM1 9131
Affinity Capture-MS Homo sapiens
155 CANX 821
Co-fractionation Homo sapiens
156 SNRPD2 6633
Co-fractionation Homo sapiens
157 AMBRA1  
Affinity Capture-MS Homo sapiens
158 C9orf78 51759
Affinity Capture-MS Homo sapiens
159 SDHA 6389
Affinity Capture-MS Homo sapiens
160 EIF4G1 1981
Co-fractionation Homo sapiens
161 YBX1 4904
Co-fractionation Homo sapiens
162 PSMD13 5719
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
163 CCT6A 908
Co-fractionation Homo sapiens
164 PSMD8 5714
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
165 PRKAR2A 5576
Co-fractionation Homo sapiens
166 LRRFIP2 9209
Co-fractionation Homo sapiens
167 U2AF2 11338
Co-fractionation Homo sapiens
168 NFS1 9054
Co-fractionation Homo sapiens
169 TRAF6 7189
Affinity Capture-MS Homo sapiens
170 CUL3 8452
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
171 GOPC 57120
Co-fractionation Homo sapiens
172 PSMA2 5683
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
173 FKBP8 23770
Affinity Capture-MS Homo sapiens
174 CIITA  
Affinity Capture-Western Homo sapiens
175 FBXO6 26270
Affinity Capture-MS Homo sapiens
176 DUSP16  
Affinity Capture-MS Homo sapiens
177 PSMD7 5713
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
178 VBP1 7411
Co-fractionation Homo sapiens
179 NXF1 10482
Affinity Capture-RNA Homo sapiens
180 TBP  
Affinity Capture-Western Homo sapiens
181 AURKB 9212
Affinity Capture-MS Homo sapiens
182 PDCD4 27250
Co-fractionation Homo sapiens
183 PRMT5 10419
Co-fractionation Homo sapiens
184 MAPK6  
Affinity Capture-MS Homo sapiens
185 DDX46 9879
Co-fractionation Homo sapiens
186 TJP2 9414
Co-fractionation Homo sapiens
187 MID1 4281
Affinity Capture-MS Homo sapiens
188 MYC  
Affinity Capture-MS Homo sapiens
Dosage Lethality Homo sapiens
189 RPA3 6119
Proximity Label-MS Homo sapiens
190 NIN 51199
Proximity Label-MS Homo sapiens
191 TXNL1 9352
Co-fractionation Homo sapiens
192 CCT2 10576
Co-fractionation Homo sapiens
193 MBD2 8932
Co-fractionation Homo sapiens
194 NDC80 10403
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
195 PSMC5 5705
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
196 SKIV2L2 23517
Co-fractionation Homo sapiens
197 PSMB5 5693
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
198 MFN2 9927
Proximity Label-MS Homo sapiens
199 DDRGK1 65992
Affinity Capture-MS Homo sapiens
200 RNF2  
Affinity Capture-MS Homo sapiens
201 KIAA0368 23392
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Affinity Capture-MS Homo sapiens
202 HSPA4 3308
Affinity Capture-Western Homo sapiens
203 PSME3 10197
Co-fractionation Homo sapiens
204 RNF185  
Affinity Capture-MS Homo sapiens
205 PRKCI 5584
Co-fractionation Homo sapiens
206 RAB5C 5878
Co-fractionation Homo sapiens
207 Psmd6 66413
Affinity Capture-MS Mus musculus
208 RIPK4  
Affinity Capture-MS Homo sapiens
209 FTSJ3 117246
Co-fractionation Homo sapiens
210 AKAP1 8165
Proximity Label-MS Homo sapiens
211 PPP2CB 5516
Co-fractionation Homo sapiens
212 HUWE1 10075
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
213 ACBD5 91452
Proximity Label-MS Homo sapiens
214 TMEM31  
Affinity Capture-MS Homo sapiens
215 PSMB3 5691
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
216 STUB1 10273
Biochemical Activity Homo sapiens
217 HSP90AB1 3326
Co-fractionation Homo sapiens
218 PSMB2 5690
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
219 TRIM5 85363
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
FRET Homo sapiens
220 RELA 5970
Affinity Capture-Western Homo sapiens
221 GRK5 2869
Affinity Capture-MS Homo sapiens
222 ZFAND2A  
Affinity Capture-MS Homo sapiens
223 RPS27A 6233
Affinity Capture-MS Homo sapiens
224 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
225 CCT8 10694
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
226 LAMTOR1 55004
Proximity Label-MS Homo sapiens
227 SKIL  
Two-hybrid Homo sapiens
228 GRB2 2885
Affinity Capture-MS Homo sapiens
229 MTMR1 8776
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
230 Psmb4 19172
Affinity Capture-MS Mus musculus
231 RPS21 6227
Co-fractionation Homo sapiens
232 PPP2R2A 5520
Co-fractionation Homo sapiens
233 PSMB7 5695
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
234 ATXN7  
Co-fractionation Homo sapiens
235 KPNA6 23633
Co-fractionation Homo sapiens
236 PSME2 5721
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
237 POMP  
Co-fractionation Homo sapiens
238 SQSTM1 8878
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
239 USP44  
Affinity Capture-MS Homo sapiens
240 SHFM1 7979
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
241 DCTN2 10540
Co-fractionation Homo sapiens
Proximity Label-MS Homo sapiens
242 PTPN2 5771
Affinity Capture-MS Homo sapiens
243 HDAC1 3065
Co-fractionation Homo sapiens
244 CAPZB 832
Affinity Capture-MS Homo sapiens
245 SUCLA2 8803
Co-fractionation Homo sapiens
246 SHMT2 6472
Affinity Capture-RNA Homo sapiens
247 MAGEA9  
Affinity Capture-MS Homo sapiens
248 CCDC74A  
Affinity Capture-MS Homo sapiens
249 MYL12A 10627
Co-fractionation Homo sapiens
250 PSMA1 5682
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
251 UBE3C 9690
Affinity Capture-MS Homo sapiens
252 ANAPC2 29882
Proximity Label-MS Homo sapiens
253 UFL1 23376
Affinity Capture-MS Homo sapiens
254 MCFD2 90411
Co-fractionation Homo sapiens
255 ANXA11 311
Cross-Linking-MS (XL-MS) Homo sapiens
256 DNM1L 10059
Affinity Capture-MS Homo sapiens
257 LIMA1 51474
Co-fractionation Homo sapiens
258 PSMA5 5686
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
259 GLI2  
Affinity Capture-MS Homo sapiens
260 IPO4 79711
Co-fractionation Homo sapiens
261 RAD21 5885
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
262 GAPVD1 26130
Co-fractionation Homo sapiens
263 COX7A2 1347
Affinity Capture-MS Homo sapiens
264 HERC3 8916
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
265 MRPS16  
Co-fractionation Homo sapiens
266 CAD 790
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
267 MAF  
Affinity Capture-MS Homo sapiens
268 SNW1 22938
Affinity Capture-MS Homo sapiens
269 CEP85  
Affinity Capture-MS Homo sapiens
270 ADRM1 11047
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
271 C9orf72  
Affinity Capture-MS Homo sapiens
272 AR 367
Affinity Capture-MS Homo sapiens
273 PSMD1 5707
Co-purification Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
274 ATXN3 4287
Affinity Capture-MS Homo sapiens
275 ATOH1  
Affinity Capture-MS Homo sapiens
276 PSMC4 5704
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
277 PTCH1  
Affinity Capture-MS Homo sapiens
278 REC8  
Affinity Capture-MS Homo sapiens
279 PRMT1 3276
Affinity Capture-MS Homo sapiens
280 LRRC57 255252
Affinity Capture-MS Homo sapiens
281 C17orf59 54785
Proximity Label-MS Homo sapiens
282 CDC5L 988
Affinity Capture-MS Homo sapiens
283 PSMD3 5709
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
284 PSME4 23198
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which PSMC2 is involved
PathwayEvidenceSource
ABC transporter disorders TAS Reactome
ABC-family proteins mediated transport TAS Reactome
Activation of APC/C and APC/C:Cdc20 mediated degradation of mitotic proteins TAS Reactome
Activation of NF-kappaB in B cells TAS Reactome
Adaptive Immune System IEA Reactome
Adaptive Immune System TAS Reactome
Antigen processing-Cross presentation IEA Reactome
Antigen processing-Cross presentation TAS Reactome
Antigen processing: Ubiquitination & Proteasome degradation TAS Reactome
APC/C-mediated degradation of cell cycle proteins TAS Reactome
APC/C:Cdc20 mediated degradation of mitotic proteins TAS Reactome
APC/C:Cdc20 mediated degradation of Securin TAS Reactome
APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1 TAS Reactome
APC:Cdc20 mediated degradation of cell cycle proteins prior to satisfation of the cell cycle checkpoint TAS Reactome
Apoptosis IEA Reactome
Assembly of the pre-replicative complex TAS Reactome
Asymmetric localization of PCP proteins TAS Reactome
AUF1 (hnRNP D0) binds and destabilizes mRNA TAS Reactome
Autodegradation of Cdh1 by Cdh1:APC/C TAS Reactome
Autodegradation of the E3 ubiquitin ligase COP1 TAS Reactome
Axon guidance IEA Reactome
Beta-catenin independent WNT signaling TAS Reactome
C-type lectin receptors (CLRs) TAS Reactome
Cdc20:Phospho-APC/C mediated degradation of Cyclin A TAS Reactome
CDK-mediated phosphorylation and removal of Cdc6 TAS Reactome
Cell Cycle TAS Reactome
Cell Cycle Checkpoints TAS Reactome
Cell Cycle, Mitotic TAS Reactome
Cellular response to chemical stress TAS Reactome
Cellular response to hypoxia TAS Reactome
Cellular responses to stimuli TAS Reactome
Cellular responses to stress TAS Reactome
Class I MHC mediated antigen processing & presentation IEA Reactome
Class I MHC mediated antigen processing & presentation TAS Reactome
CLEC7A (Dectin-1) signaling TAS Reactome
Cross-presentation of soluble exogenous antigens (endosomes) TAS Reactome
Cyclin A:Cdk2-associated events at S phase entry TAS Reactome
Cyclin E associated events during G1/S transition TAS Reactome
Cytokine Signaling in Immune system TAS Reactome
Dectin-1 mediated noncanonical NF-kB signaling TAS Reactome
Defective CFTR causes cystic fibrosis TAS Reactome
Degradation of AXIN TAS Reactome
Degradation of beta-catenin by the destruction complex TAS Reactome
Degradation of DVL TAS Reactome
Degradation of GLI1 by the proteasome TAS Reactome
Degradation of GLI2 by the proteasome IEA Reactome
Deubiquitination TAS Reactome
Developmental Biology IEA Reactome
Disease TAS Reactome
Diseases of signal transduction by growth factor receptors and second messengers TAS Reactome
Disorders of transmembrane transporters TAS Reactome
DNA Replication TAS Reactome
DNA Replication Pre-Initiation TAS Reactome
Downstream signaling events of B Cell Receptor (BCR) TAS Reactome
Downstream TCR signaling TAS Reactome
ER-Phagosome pathway IEA Reactome
FBXL7 down-regulates AURKA during mitotic entry and in early mitosis TAS Reactome
Fc epsilon receptor (FCERI) signaling TAS Reactome
FCERI mediated NF-kB activation TAS Reactome
Formation of paraxial mesoderm IEA Reactome
G1/S DNA Damage Checkpoints TAS Reactome
G1/S Transition TAS Reactome
G2/M Checkpoints TAS Reactome
G2/M Transition TAS Reactome
Gastrulation IEA Reactome
Gene expression (Transcription) TAS Reactome
Gene expression (Transcription) IEA Reactome
Generic Transcription Pathway TAS Reactome
Generic Transcription Pathway IEA Reactome
GLI3 is processed to GLI3R by the proteasome TAS Reactome
GSK3B and BTRC:CUL1-mediated-degradation of NFE2L2 TAS Reactome
Hedgehog 'off' state IEA Reactome
Hedgehog 'off' state TAS Reactome
Hedgehog 'on' state TAS Reactome
Hedgehog ligand biogenesis TAS Reactome
Hh mutants abrogate ligand secretion TAS Reactome
Hh mutants are degraded by ERAD TAS Reactome
HIV Infection TAS Reactome
Host Interactions of HIV factors TAS Reactome
Immune System IEA Reactome
Immune System TAS Reactome
Infectious disease TAS Reactome
Innate Immune System TAS Reactome
Interleukin-1 family signaling TAS Reactome
Interleukin-1 signaling TAS Reactome
Intracellular signaling by second messengers TAS Reactome
KEAP1-NFE2L2 pathway TAS Reactome
M Phase TAS Reactome
MAPK family signaling cascades TAS Reactome
MAPK1/MAPK3 signaling TAS Reactome
MAPK6/MAPK4 signaling TAS Reactome
Metabolism TAS Reactome
Metabolism of amino acids and derivatives TAS Reactome
Metabolism of polyamines TAS Reactome
Metabolism of proteins TAS Reactome
Metabolism of proteins IEA Reactome
Metabolism of RNA TAS Reactome
Mitotic Anaphase TAS Reactome
Mitotic G1 phase and G1/S transition TAS Reactome
Mitotic G2-G2/M phases TAS Reactome
Mitotic Metaphase and Anaphase TAS Reactome
Neddylation TAS Reactome
Negative regulation of NOTCH4 signaling IEA Reactome
Nervous system development IEA Reactome
Neutrophil degranulation TAS Reactome
NIK-->noncanonical NF-kB signaling TAS Reactome
Nuclear events mediated by NFE2L2 TAS Reactome
Orc1 removal from chromatin TAS Reactome
Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha TAS Reactome
p53-Dependent G1 DNA Damage Response TAS Reactome
p53-Dependent G1/S DNA damage checkpoint TAS Reactome
p53-Independent DNA Damage Response TAS Reactome
p53-Independent G1/S DNA damage checkpoint TAS Reactome
PCP/CE pathway TAS Reactome
PIP3 activates AKT signaling TAS Reactome
Post-translational protein modification TAS Reactome
Post-translational protein modification IEA Reactome
Programmed Cell Death IEA Reactome
Proteasome assembly TAS Reactome
Proteasome assembly IEA Reactome
PTEN Regulation TAS Reactome
RAF/MAP kinase cascade TAS Reactome
Regulation of activated PAK-2p34 by proteasome mediated degradation IEA Reactome
Regulation of APC/C activators between G1/S and early anaphase TAS Reactome
Regulation of Apoptosis IEA Reactome
Regulation of expression of SLITs and ROBOs IEA Reactome
Regulation of mitotic cell cycle TAS Reactome
Regulation of mRNA stability by proteins that bind AU-rich elements TAS Reactome
Regulation of ornithine decarboxylase (ODC) TAS Reactome
Regulation of PTEN stability and activity TAS Reactome
Regulation of RAS by GAPs TAS Reactome
Regulation of RUNX2 expression and activity IEA Reactome
Regulation of RUNX2 expression and activity TAS Reactome
Regulation of RUNX3 expression and activity TAS Reactome
RNA Polymerase II Transcription TAS Reactome
RNA Polymerase II Transcription IEA Reactome
RUNX1 regulates transcription of genes involved in differentiation of HSCs TAS Reactome
S Phase TAS Reactome
SCF(Skp2)-mediated degradation of p27/p21 TAS Reactome
SCF-beta-TrCP mediated degradation of Emi1 TAS Reactome
Separation of Sister Chromatids TAS Reactome
Signal Transduction TAS Reactome
Signal Transduction IEA Reactome
Signaling by Hedgehog TAS Reactome
Signaling by Hedgehog IEA Reactome
Signaling by Interleukins TAS Reactome
Signaling by NOTCH IEA Reactome
Signaling by NOTCH4 IEA Reactome
Signaling by ROBO receptors IEA Reactome
Signaling by the B Cell Receptor (BCR) TAS Reactome
Signaling by WNT TAS Reactome
Somitogenesis IEA Reactome
Stabilization of p53 TAS Reactome
Switching of origins to a post-replicative state TAS Reactome
Synthesis of DNA TAS Reactome
TCF dependent signaling in response to WNT TAS Reactome
TCR signaling TAS Reactome
The role of GTSE1 in G2/M progression after G2 checkpoint TAS Reactome
TNFR2 non-canonical NF-kB pathway TAS Reactome
Transcriptional regulation by RUNX1 TAS Reactome
Transcriptional regulation by RUNX2 IEA Reactome
Transcriptional regulation by RUNX2 TAS Reactome
Transcriptional regulation by RUNX3 TAS Reactome
Transport of small molecules TAS Reactome
Ub-specific processing proteases TAS Reactome
Ubiquitin Mediated Degradation of Phosphorylated Cdc25A TAS Reactome
Ubiquitin-dependent degradation of Cyclin D TAS Reactome
UCH proteinases TAS Reactome
Vif-mediated degradation of APOBEC3G TAS Reactome
Viral Infection Pathways TAS Reactome
Vpu mediated degradation of CD4 TAS Reactome





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