Experiment description of studies that identified Flot2 in exosomes

1 Experiment ID 65 ISEV standards ✘ EV Biophysical techniques ✔ HSP60|HSP27|GAPDH EV Cytosolic markers ✔ FLOT2 EV Membrane markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Western blotting PubMed ID 19415654     Organism Homo sapiens Experiment description Proteomics of MUC1-containing lipid rafts from plasma membranes and exosomes of human breast carcinoma cells MCF-7. Authors Staubach S, Razawi H, Hanisch FG. Journal name PROTEOMICS Publication year 2009 Sample Breast cancer cells Sample name MCF-7 Isolation/purification methods Differential centrifugation Sucrose density gradient Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [MALDI TOF] Western blotting

2 Experiment ID 130 ISEV standards ✔ EM EV Biophysical techniques ✔ Alix EV Cytosolic markers ✔ FLOT2 EV Membrane markers ✔ CANX|GOLGA2 EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Western blotting PubMed ID 18309083     Organism Mus musculus Experiment description Ceramide triggers budding of exosome vesicles into multivesicular endosomes Authors Trajkovic K, Hsu C, Chiantia S, Rajendran L, Wenzel D, Wieland F, Schwille P, Brugger B, Simons M. Journal name SCIENCE Publication year 2008 Sample Oligodendrocytes Sample name Oli-neu cells Isolation/purification methods Differential centrifugation Sucrose density gradient Flotation density - Molecules identified in the study Protein Lipids Methods used in the study Western blotting Mass spectrometry [QTOF]

Protein-protein interactions for Flot2

Protein Interactor ExoCarta ID Identification method PubMed Species No interactions are found.

Pathways in which Flot2 is involved

No pathways found