Gene description for MAPKAP1
Gene name mitogen-activated protein kinase associated protein 1
Gene symbol MAPKAP1
Other names/aliases JC310
MIP1
SIN1
SIN1b
SIN1g
Species Homo sapiens
 Database cross references - MAPKAP1
ExoCarta ExoCarta_79109
Entrez Gene 79109
HGNC 18752
MIM 610558
UniProt Q9BPZ7  
 MAPKAP1 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for MAPKAP1
Molecular Function
    protein kinase binding GO:0019901 IPI
    phosphatidylinositol-3,4-bisphosphate binding GO:0043325 IDA
    phosphatidylinositol-3,4,5-trisphosphate binding GO:0005547 IDA
    Ras GTPase binding GO:0017016 IDA
    phosphatidylinositol-3,5-bisphosphate binding GO:0080025 IDA
    protein binding GO:0005515 IPI
    phosphatidic acid binding GO:0070300 IDA
    phosphatidylinositol-4,5-bisphosphate binding GO:0005546 IDA
Biological Process
    negative regulation of Ras protein signal transduction GO:0046580 IMP
    phosphatidylinositol-mediated signaling GO:0048015 TAS
    Fc-epsilon receptor signaling pathway GO:0038095 TAS
    innate immune response GO:0045087 TAS
    neurotrophin TRK receptor signaling pathway GO:0048011 TAS
    substantia nigra development GO:0021762 IEP
    vascular endothelial growth factor receptor signaling pathway GO:0048010 TAS
    T cell costimulation GO:0031295 TAS
    epidermal growth factor receptor signaling pathway GO:0007173 TAS
    fibroblast growth factor receptor signaling pathway GO:0008543 TAS
Subcellular Localization
    plasma membrane GO:0005886 IDA
    nucleoplasm GO:0005654 IDA
    cytoplasmic membrane-bounded vesicle GO:0016023 IEA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
    nucleus GO:0005634 IDA
    Golgi apparatus GO:0005794 IDA
 Experiment description of studies that identified MAPKAP1 in exosomes
1
Experiment ID 235
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 236
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for MAPKAP1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SFN 2810
Affinity Capture-MS Homo sapiens
2 RPTOR 57521
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 AKT1 207
Affinity Capture-Western Homo sapiens
4 MTOR 2475
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
5 RICTOR 253260
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
6 CDC42EP1  
Two-hybrid Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here