Experiment description of studies that identified BAG6 in exosomes

1 Experiment ID 207 ISEV standards ✔ EM EV Biophysical techniques ✔ TSG101|HSP70 EV Cytosolic markers ✔ FLOT1 EV Membrane markers ✔ VDAC EV Negative markers ✔ NTA EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23161513     Organism Homo sapiens Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ. Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name DKO-1 Isolation/purification methods Differential centrifugation Filtration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

2 Experiment ID 208 ISEV standards ✔ EM EV Biophysical techniques ✔ TSG101|HSP70 EV Cytosolic markers ✔ FLOT1 EV Membrane markers ✔ VDAC EV Negative markers ✔ NTA EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23161513     Organism Homo sapiens Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ. Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name Dks-8 Isolation/purification methods Differential centrifugation Filtration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

3 Experiment ID 209 ISEV standards ✘ EV Biophysical techniques ✔ TSG101|HSP70 EV Cytosolic markers ✔ FLOT1 EV Membrane markers ✔ VDAC EV Negative markers ✔ NTA EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23161513     Organism Homo sapiens Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ. Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name DLD-1 Isolation/purification methods Differential centrifugation Filtration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

4 Experiment ID 211 ISEV standards ✔ EM EV Biophysical techniques ✔ TSG101|Alix EV Cytosolic markers ✔ EpCAM|TFRC EV Membrane markers ✔ cytochrome c|GOLGA2 EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23333927     Organism Homo sapiens Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes. Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K. Journal name J Proteomics Publication year 2013 Sample Ovarian cancer cells Sample name IGROV1 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.09-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

5 Experiment ID 212 ISEV standards ✔ CEM EV Biophysical techniques ✔ TSG101|Alix EV Cytosolic markers ✔ EpCAM|TFRC EV Membrane markers ✔ Cytochrome C|GOLGA2 EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23333927     Organism Homo sapiens Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes. Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K. Journal name J Proteomics Publication year 2013 Sample Ovarian cancer cells Sample name OVCAR-3 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.09-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

Protein-protein interactions for BAG6

Protein Interactor ExoCarta ID Identification method PubMed Species 1 DPT   Two-hybrid Homo sapiens 2 IMMT 10989 Two-hybrid Homo sapiens 3 MAGED1   Affinity Capture-MS Homo sapiens 4 ARPC3 10094 Two-hybrid Homo sapiens 5 IKBKAP 8518 Two-hybrid Homo sapiens 6 RNF126   Affinity Capture-MS Homo sapiens 7 CTSE   Two-hybrid Homo sapiens 8 PPP4C 5531 Affinity Capture-MS Homo sapiens 9 WDR8   Affinity Capture-MS Homo sapiens 10 REG1B   Two-hybrid Homo sapiens 11 NDUFA6   Two-hybrid Homo sapiens 12 SMN1 6606 Two-hybrid Homo sapiens 13 NBL1 4681 Two-hybrid Homo sapiens Affinity Capture-MS Homo sapiens 14 PPAP2C 8612 Two-hybrid Homo sapiens 15 PRNP 5621 Two-hybrid Homo sapiens 16 HSPA1A   Affinity Capture-MS Homo sapiens Invivo Homo sapiens 17 CHN2   Two-hybrid Homo sapiens 18 PTH   Two-hybrid Homo sapiens 19 EDN1   Two-hybrid Homo sapiens 20 NOMO1 23420 Two-hybrid Homo sapiens 21 TOMM20 9804 Two-hybrid Homo sapiens 22 IGFBP5 3488 Two-hybrid Homo sapiens 23 IQCG 84223 Two-hybrid Homo sapiens 24 PRPF40A 55660 Affinity Capture-MS Homo sapiens 25 SETDB1   Two-hybrid Homo sapiens 26 XRN1 54464 Two-hybrid Homo sapiens 27 C16orf24   Two-hybrid Homo sapiens 28 ZFYVE9   Affinity Capture-MS Homo sapiens 29 RAB8A 4218 Two-hybrid Homo sapiens 30 S100A4 6275 Two-hybrid Homo sapiens 31 MYDGF 56005 Two-hybrid Homo sapiens 32 PRG2   Two-hybrid Homo sapiens 33 CDK4 1019 Two-hybrid Homo sapiens 34 B2M 567 Two-hybrid Homo sapiens 35 TSC22D1   Affinity Capture-MS Homo sapiens 36 FAM46A   Affinity Capture-MS Homo sapiens 37 UBL7   Affinity Capture-MS Homo sapiens 38 RCN2 5955 Two-hybrid Homo sapiens 39 TAC1   Two-hybrid Homo sapiens 40 SGTA 6449 Affinity Capture-MS Homo sapiens 41 TDGF1   Two-hybrid Homo sapiens 42 STX5A   Two-hybrid Homo sapiens 43 PELP1 27043 Two-hybrid Homo sapiens 44 CTSB 1508 Two-hybrid Homo sapiens 45 KLHL12   Two-hybrid Homo sapiens 46 CSTF2   Two-hybrid Homo sapiens 47 FKBP2   Two-hybrid Homo sapiens 48 DNAJB11 51726 Two-hybrid Homo sapiens 49 EFEMP2 30008 Two-hybrid Homo sapiens View the network

Pathways in which BAG6 is involved

No pathways found