Gene description for BAG6
Gene name BCL2-associated athanogene 6
Gene symbol BAG6
Other names/aliases BAG-6
BAT3
D6S52E
G3
Species Homo sapiens
 Database cross references - BAG6
ExoCarta ExoCarta_7917
Entrez Gene 7917
HGNC 13919
MIM 142590
UniProt P46379  
 BAG6 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for BAG6
Molecular Function
    ribosome binding GO:0043022 IDA
    ubiquitin protein ligase binding GO:0031625 IPI
    protein binding GO:0005515 IPI
    proteasome binding GO:0070628 ISS
    polyubiquitin binding GO:0031593 ISS
    Hsp70 protein binding GO:0030544 IEA
Biological Process
    lung development GO:0030324 ISS
    cell differentiation GO:0030154 IEA
    tail-anchored membrane protein insertion into ER membrane GO:0071816 IDA
    ubiquitin-dependent protein catabolic process GO:0006511 ISS
    chromatin modification GO:0016568 IEA
    negative regulation of proteolysis GO:0045861 ISS
    intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress GO:0070059 ISS
    internal peptidyl-lysine acetylation GO:0018393 IDA
    regulation of cell proliferation GO:0042127 IEA
    transport GO:0006810 IEA
    negative regulation of apoptotic process GO:0043066 IEA
    embryo development GO:0009790 ISS
    negative regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032435 ISS
    immune system process GO:0002376 IEA
    synaptonemal complex assembly GO:0007130 ISS
    protein stabilization GO:0050821 ISS
    spermatogenesis GO:0007283 ISS
    brain development GO:0007420 ISS
    intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator GO:0042771 IMP
    kidney development GO:0001822 ISS
Subcellular Localization
    intracellular membrane-bounded organelle GO:0043231 IDA
    cytoplasm GO:0005737 IDA
    BAT3 complex GO:0071818 IDA
    cytosol GO:0005829 IDA
    nucleoplasm GO:0005654 IDA
    nucleus GO:0005634 IDA
 Experiment description of studies that identified BAG6 in exosomes
1
Experiment ID 207
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
ISEV standards
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for BAG6
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DPT  
Two-hybrid Homo sapiens
2 IMMT 10989
Two-hybrid Homo sapiens
3 MAGED1  
Affinity Capture-MS Homo sapiens
4 ARPC3 10094
Two-hybrid Homo sapiens
5 IKBKAP 8518
Two-hybrid Homo sapiens
6 RNF126  
Affinity Capture-MS Homo sapiens
7 CTSE  
Two-hybrid Homo sapiens
8 PPP4C 5531
Affinity Capture-MS Homo sapiens
9 WDR8  
Affinity Capture-MS Homo sapiens
10 REG1B  
Two-hybrid Homo sapiens
11 NDUFA6  
Two-hybrid Homo sapiens
12 SMN1 6606
Two-hybrid Homo sapiens
13 NBL1 4681
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
14 PPAP2C 8612
Two-hybrid Homo sapiens
15 PRNP 5621
Two-hybrid Homo sapiens
16 HSPA1A  
Affinity Capture-MS Homo sapiens
Invivo Homo sapiens
17 CHN2  
Two-hybrid Homo sapiens
18 PTH  
Two-hybrid Homo sapiens
19 EDN1  
Two-hybrid Homo sapiens
20 NOMO1 23420
Two-hybrid Homo sapiens
21 TOMM20 9804
Two-hybrid Homo sapiens
22 IGFBP5 3488
Two-hybrid Homo sapiens
23 IQCG 84223
Two-hybrid Homo sapiens
24 PRPF40A 55660
Affinity Capture-MS Homo sapiens
25 SETDB1  
Two-hybrid Homo sapiens
26 XRN1 54464
Two-hybrid Homo sapiens
27 C16orf24  
Two-hybrid Homo sapiens
28 ZFYVE9  
Affinity Capture-MS Homo sapiens
29 RAB8A 4218
Two-hybrid Homo sapiens
30 S100A4 6275
Two-hybrid Homo sapiens
31 MYDGF 56005
Two-hybrid Homo sapiens
32 PRG2  
Two-hybrid Homo sapiens
33 CDK4 1019
Two-hybrid Homo sapiens
34 B2M 567
Two-hybrid Homo sapiens
35 TSC22D1  
Affinity Capture-MS Homo sapiens
36 FAM46A  
Affinity Capture-MS Homo sapiens
37 UBL7  
Affinity Capture-MS Homo sapiens
38 RCN2 5955
Two-hybrid Homo sapiens
39 TAC1  
Two-hybrid Homo sapiens
40 SGTA 6449
Affinity Capture-MS Homo sapiens
41 TDGF1  
Two-hybrid Homo sapiens
42 STX5A  
Two-hybrid Homo sapiens
43 PELP1 27043
Two-hybrid Homo sapiens
44 CTSB 1508
Two-hybrid Homo sapiens
45 KLHL12  
Two-hybrid Homo sapiens
46 CSTF2  
Two-hybrid Homo sapiens
47 FKBP2  
Two-hybrid Homo sapiens
48 DNAJB11 51726
Two-hybrid Homo sapiens
49 EFEMP2 30008
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which BAG6 is involved
No pathways found





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here