Gene description for MCL1
Gene name myeloid cell leukemia 1
Gene symbol MCL1
Other names/aliases BCL2L3
EAT
MCL1-ES
MCL1L
MCL1S
Mcl-1
TM
bcl2-L-3
mcl1/EAT
Species Homo sapiens
 Database cross references - MCL1
ExoCarta ExoCarta_4170
Entrez Gene 4170
HGNC 6943
MIM 159552
UniProt Q07820  
 MCL1 identified in exosomes derived from the following tissue/cell type
Endothelial cells 26027894    
 Gene ontology annotations for MCL1
Molecular Function
    protein heterodimerization activity GO:0046982 IPI
    BH3 domain binding GO:0051434 IPI
    protein channel activity GO:0015266 TAS
    protein binding GO:0005515 IPI
    protein homodimerization activity GO:0042803 IBA
Biological Process
    multicellular organismal development GO:0007275 IEA
    apoptotic mitochondrial changes GO:0008637 IEA
    cellular homeostasis GO:0019725 NAS
    intrinsic apoptotic signaling pathway in response to DNA damage GO:0008630 IBA
    response to cytokine GO:0034097 IDA
    negative regulation of intrinsic apoptotic signaling pathway GO:2001243 IBA
    regulation of response to DNA damage stimulus GO:2001020 IMP
    extrinsic apoptotic signaling pathway in absence of ligand GO:0097192 IMP
    cell fate determination GO:0001709 NAS
    negative regulation of anoikis GO:2000811 IMP
    negative regulation of extrinsic apoptotic signaling pathway in absence of ligand GO:2001240 IMP
    protein transmembrane transport GO:0071806 TAS
    positive regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway GO:1903378 IGI
Subcellular Localization
    membrane GO:0016020 ISS
    cytoplasm GO:0005737 TAS
    nucleus GO:0005634 IDA
    integral component of membrane GO:0016021 IEA
    mitochondrion GO:0005739 IDA
    Bcl-2 family protein complex GO:0097136 IDA
    mitochondrial matrix GO:0005759 IEA
    mitochondrial outer membrane GO:0005741 TAS
    cytosol GO:0005829 ISS
    nucleoplasm GO:0005654 IEA
 Experiment description of studies that identified MCL1 in exosomes
1
Experiment ID 226
ISEV standards
EM
EV Biophysical techniques
GAPDH
EV Cytosolic markers
CD9|FLOT1
EV Membrane markers
LMNA|H2AFX|ATP5A1|TOMM20
EV Negative markers
EV Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting
Authors Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
 Protein-protein interactions for MCL1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 BID  
Protein-peptide Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
2 PCNA 5111
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
3 MCL1 4170
Invivo Homo sapiens
Invivo Homo sapiens
4 BAD  
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Protein-peptide Homo sapiens
5 VDAC1 7416
Reconstituted Complex Homo sapiens
6 BOK  
Two-hybrid Homo sapiens
7 BMF  
Protein-peptide Homo sapiens
8 DAD1 1603
Invivo Homo sapiens
9 HRK  
Protein-peptide Homo sapiens
10 BAK1  
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Protein-peptide Homo sapiens
Reconstituted Complex Homo sapiens
11 BCL2L11  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
12 Bcl2l11  
Protein-peptide Mus musculus
13 PMAIP1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
14 CASP3 836
Biochemical Activity Homo sapiens
15 BAX 581
Invitro Homo sapiens
16 BBC3  
Affinity Capture-Western Homo sapiens
17 BIK  
Protein-peptide Homo sapiens
18 TNKS 8658
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which MCL1 is involved
No pathways found





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