Gene description for FAS
Gene name Fas cell surface death receptor
Gene symbol FAS
Other names/aliases ALPS1A
APO-1
APT1
CD95
FAS1
FASTM
TNFRSF6
Species Homo sapiens
 Database cross references - FAS
ExoCarta ExoCarta_355
Entrez Gene 355
HGNC 11920
MIM 134637
UniProt P25445  
 FAS identified in exosomes derived from the following tissue/cell type
B cells 20458337    
B cells 20458337    
B cells 20458337    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for FAS
Molecular Function
    signal transducer activity GO:0004871 TAS
    receptor activity GO:0004872 NAS
    identical protein binding GO:0042802 IPI
    transmembrane signaling receptor activity GO:0004888 IEA
    kinase binding GO:0019900 IPI
    protein binding GO:0005515 IPI
Biological Process
    regulation of necrotic cell death GO:0010939 TAS
    extrinsic apoptotic signaling pathway via death domain receptors GO:0008625 IEA
    negative thymic T cell selection GO:0045060 IEA
    regulation of myeloid cell differentiation GO:0045637 IEA
    positive regulation of extrinsic apoptotic signaling pathway in absence of ligand GO:2001241 IEA
    regulation of extrinsic apoptotic signaling pathway via death domain receptors GO:1902041 TAS
    renal system process GO:0003014 IEA
    negative regulation of B cell activation GO:0050869 IEA
    signal transduction GO:0007165 TAS
    B cell mediated immunity GO:0019724 IEA
    inflammatory cell apoptotic process GO:0006925 IEA
    immunoglobulin production GO:0002377 IEA
    extrinsic apoptotic signaling pathway in absence of ligand GO:0097192 IEA
    activation of cysteine-type endopeptidase activity involved in apoptotic process GO:0006919 TAS
    response to toxic substance GO:0009636 IEA
    positive regulation of protein homooligomerization GO:0032464 IEA
    cellular response to mechanical stimulus GO:0071260 IEP
    hepatocyte apoptotic process GO:0097284 IEA
    activation-induced cell death of T cells GO:0006924 IEA
    regulation of apoptotic process GO:0042981 NAS
    protein homooligomerization GO:0051260 IEA
    spleen development GO:0048536 IEA
    necroptotic process GO:0070266 TAS
    circadian rhythm GO:0007623 IEA
    cell surface receptor signaling pathway GO:0007166 TAS
    apoptotic process GO:0006915 TAS
    apoptotic signaling pathway GO:0097190 TAS
    transformed cell apoptotic process GO:0006927 IEA
    cellular response to lithium ion GO:0071285 IEA
    protein complex assembly GO:0006461 TAS
    programmed cell death GO:0012501 TAS
    cellular response to hyperoxia GO:0071455 IMP
    motor neuron apoptotic process GO:0097049 IEA
    necroptotic signaling pathway GO:0097527 IMP
    programmed necrotic cell death GO:0097300 TAS
    negative regulation of cysteine-type endopeptidase activity involved in apoptotic process GO:0043154 TAS
    positive regulation of apoptotic process GO:0043065 IMP
    extrinsic apoptotic signaling pathway GO:0097191 TAS
    regulation of lymphocyte differentiation GO:0045619 IEA
    activation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway GO:0097296 TAS
    negative regulation of apoptotic process GO:0043066 TAS
    response to glucocorticoid GO:0051384 IEA
Subcellular Localization
    extracellular exosome GO:0070062 IDA
    cytosol GO:0005829 NAS
    membrane raft GO:0045121 IDA
    cell surface GO:0009986 IDA
    death-inducing signaling complex GO:0031264 IDA
    nucleus GO:0005634 IDA
    plasma membrane GO:0005886 TAS
    integral component of membrane GO:0016021 IEA
    cytoplasm GO:0005737 IDA
    external side of plasma membrane GO:0009897 IEA
    CD95 death-inducing signaling complex GO:0031265 IDA
 Experiment description of studies that identified FAS in exosomes
1
Experiment ID 79
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
CD81|MHCII
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1
Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
2
Experiment ID 80
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
CD81|MHCII
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2
Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
3
Experiment ID 81
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
CD81|MHCII
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3
Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
4
Experiment ID 207
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 208
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 209
ISEV standards
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for FAS
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PDCD6 10016
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
2 TNFRSF1A 7132
Co-purification Homo sapiens
3 FADD 8772
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Co-purification Homo sapiens
4 CFLAR  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
5 EEF1A1 1915
Two-hybrid Homo sapiens
6 C14orf1 11161
Two-hybrid Homo sapiens
7 FAS 355
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
8 DAXX  
Invivo Homo sapiens
Invitro Homo sapiens
9 RIPK1  
Two-hybrid Homo sapiens
10 RIF1  
Two-hybrid Homo sapiens
11 CASP8  
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
12 EZR 7430
Co-purification Homo sapiens
13 HIPK3 10114
Affinity Capture-Western Homo sapiens
14 FASLG 356
Affinity Capture-Western Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Co-purification Homo sapiens
Reconstituted Complex Homo sapiens
15 LCK 3932
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
16 TRADD 8717
Invivo Homo sapiens
17 BID  
Co-purification Homo sapiens
18 TNFRSF10B 8795
Co-purification Homo sapiens
19 ARHGDIA 396
Co-purification Homo sapiens
20 TNFSF13 8741
Invitro Homo sapiens
21 UBE2I 7329
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
22 FYN 2534
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
23 CASP10  
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
24 TNFSF12-TNFSF13  
Invitro Homo sapiens
25 FAIM2  
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
26 BTK 695
Invivo Homo sapiens
27 CASP8AP2  
Invivo Homo sapiens
28 FBF1  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
29 MSN 4478
Co-purification Homo sapiens
30 CRMP1 1400
Two-hybrid Homo sapiens
31 RHOA 387
Co-purification Homo sapiens
32 MAPK8 5599
Co-purification Homo sapiens
33 FAF1 11124
Invivo Homo sapiens
34 SUMO1 7341
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which FAS is involved
PathwayEvidenceSource
FasL/ CD95L signaling TAS Reactome





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