|
 Gene ontology annotations for TSG101 |
|
|
| Experiment description of studies that identified TSG101 in sEVs |
| 1 |
| Experiment ID |
116 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
21505438
|
| Organism |
Homo sapiens |
| Experiment description |
Unidirectional transfer of microRNA-loaded exosomes from T cells to antigen-presenting cells. |
| Authors |
"Mittelbrunn M, Gutierrez-Vázquez C, Villarroya-Beltri C, González S, Sánchez-Cabo F, González MÃÂ, Bernad A, Sánchez-Madrid F." |
| Journal name |
NATCOM
|
| Publication year |
2011 |
| Sample |
B cells |
| Sample name |
Raji |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
miRNA |
| Methods used in the study |
Mass spectrometry
Microarray |
|
|
| 2 |
| Experiment ID |
76 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
TSG101|GAPDH|HSP90|CD81|CD9|CD63|LAMP1|MHCI
|
Enriched markers |
|
✔
HSP90B1
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
20224111
|
| Organism |
Homo sapiens |
| Experiment description |
Proteomics analysis of bladder cancer exosomes. |
| Authors |
"Welton JL, Khanna S, Giles PJ, Brennan P, Brewis IA, Staffurth J, Mason MD, Clayton A." |
| Journal name |
MCP
|
| Publication year |
2010 |
| Sample |
Bladder cancer cells |
| Sample name |
HT1376 |
| Isolation/purification methods |
Differential centrifugation
Sucrose density gradient |
| Flotation density |
1.10-1.19 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry [MALDI TOF/TOF]
Western blotting
FACS |
|
|
| 3 |
| Experiment ID |
627 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
TSG101
|
Enriched markers |
|
✔
GOLGA2|ALB
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Western blotting
|
| PubMed ID |
31489147
|
| Organism |
Homo sapiens |
| Experiment description |
Inflammation potentiates miR-939 expression and packaging into small extracellular vesicles |
| Authors |
"Ramanathan S, Shenoda BB, Lin Z, Alexander GM, Huppert A, Sacan A, Ajit SK." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2019 |
| Sample |
Blood |
| Sample name |
PBMC from CRPS patients |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 4 |
| Experiment ID |
489 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
|
Enriched markers |
|
✔
Canx
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
36408942
|
| Organism |
Rattus norvegicus |
| Experiment description |
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells |
| Authors |
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ." |
| Journal name |
Proteomics
|
| Publication year |
2023 |
| Sample |
Bone marrow mesenchymal stem cells |
| Sample name |
BMSC - Passage 6 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectometry |
|
|
| 5 |
| Experiment ID |
490 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
|
Enriched markers |
|
✔
Canx
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
36408942
|
| Organism |
Rattus norvegicus |
| Experiment description |
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells |
| Authors |
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ." |
| Journal name |
Proteomics
|
| Publication year |
2023 |
| Sample |
Bone marrow mesenchymal stem cells |
| Sample name |
BMSC - Passage 7 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectometry |
|
|
| 6 |
| Experiment ID |
491 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
|
Enriched markers |
|
✔
Canx
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
36408942
|
| Organism |
Rattus norvegicus |
| Experiment description |
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells |
| Authors |
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ." |
| Journal name |
Proteomics
|
| Publication year |
2023 |
| Sample |
Bone marrow mesenchymal stem cells |
| Sample name |
BMSC - Passage 8 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectometry |
|
|
| 7 |
| Experiment ID |
492 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
|
Enriched markers |
|
✔
Canx
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
36408942
|
| Organism |
Rattus norvegicus |
| Experiment description |
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells |
| Authors |
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ." |
| Journal name |
Proteomics
|
| Publication year |
2023 |
| Sample |
Bone marrow mesenchymal stem cells |
| Sample name |
BMSC - Passage 9 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectometry |
|
|
| 8 |
| Experiment ID |
758 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
TSG101|CD63
|
Enriched markers |
|
✔
CANX|GOLGA2
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35351860
|
| Organism |
Homo sapiens |
| Experiment description |
Bone marrow mesenchymal stem cell-derived exosomal microRNA-381-3p alleviates vascular calcification in chronic kidney disease by targeting NFAT5 |
| Authors |
"Liu Y, Guo Y, Bao S, Huang H, Liu W, Guo W." |
| Journal name |
Cell Death Dis
|
| Publication year |
2022 |
| Sample |
Bone marrow mesenchymal stem cells |
| Sample name |
BMSC |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
miRNA |
| Methods used in the study |
Western blotting
RT-qPCR |
|
|
| 9 |
| Experiment ID |
227 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
Alix|TSG101
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
25833198
|
| Organism |
Homo sapiens |
| Experiment description |
Guggulsterone and bexarotene induce secretion of exosome-associated breast cancer resistance protein and reduce doxorubicin resistance in MDA-MB-231 cells |
| Authors |
"Kong JN, He Q, Wang G, Dasgupta S, Dinkins MB, Zhu G, Kim A, Spassieva S, Bieberich E." |
| Journal name |
International Journal of Cancer
|
| Publication year |
2015 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 |
| Isolation/purification methods |
Differential ultracentrifugation
Sucrose density gradient |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western Blotting |
|
|
| 10 |
| Experiment ID |
397 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|TSG101|CD63
|
Enriched markers |
|
✔
GOLGA2|CANX
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
33738083
|
| Organism |
Homo sapiens |
| Experiment description |
Bioorthogonally surface-edited extracellular vesicles based on metabolic glycoengineering for CD44-mediated targeting of inflammatory diseases |
| Authors |
"Lim GT, You DG, Han HS, Lee H, Shin S, Oh BH, Kumar EKP, Um W, Kim CH, Han S, Lee S, Lim S, Yoon HY, Kim K, Kwon IC, Jo DG, Cho YW, Park JH" |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2021 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Immunofluorescence
Flow cytomtery |
|
|
| 11 |
| Experiment ID |
398 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|TSG101|CD63
|
Enriched markers |
|
✔
GOLGA2|CANX
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
33738083
|
| Organism |
Homo sapiens |
| Experiment description |
Bioorthogonally surface-edited extracellular vesicles based on metabolic glycoengineering for CD44-mediated targeting of inflammatory diseases |
| Authors |
"Lim GT, You DG, Han HS, Lee H, Shin S, Oh BH, Kumar EKP, Um W, Kim CH, Han S, Lee S, Lim S, Yoon HY, Kim K, Kwon IC, Jo DG, Cho YW, Park JH" |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2021 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Immunofluorescence
Flow cytomtery |
|
|
| 12 |
| Experiment ID |
412 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
|
Enriched markers |
|
✔
CANX|ACTB
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Breast cancer cells |
| Sample name |
MCF7 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
mRNA |
| Methods used in the study |
RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry |
|
|
| 13 |
| Experiment ID |
414 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
mRNA |
| Methods used in the study |
RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry |
|
|
| 14 |
| Experiment ID |
426 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 - Exo-rich fractions 7-10 pooled |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 15 |
| Experiment ID |
427 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 - Exo-rich fractions 1-6 pooled |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
| 16 |
| Experiment ID |
458 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD81|TSG101
|
Enriched markers |
|
✔
CALR
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36517516
|
| Organism |
Homo sapiens |
| Experiment description |
Exosomal miR-1304-3p promotes breast cancer progression in African Americans by activating cancer-associated adipocytes |
| Authors |
"Zhao D, Wu K, Sharma S, Xing F, Wu SY, Tyagi A, Deshpande R, Singh R, Wabitsch M, Mo YY, Watabe K." |
| Journal name |
Nat Commun
|
| Publication year |
2022 |
| Sample |
Breast cancer cells |
| Sample name |
HCC1806 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
miRNA |
| Methods used in the study |
Western blotting
qPCR |
|
|
| 17 |
| Experiment ID |
679 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
FLOT1|TSG101
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35939247
|
| Organism |
Homo sapiens |
| Experiment description |
Metastasis suppressor NME1 in exosomes or liposomes conveys motility and migration inhibition in breast cancer model systems |
| Authors |
"Khan I, Gril B, Hoshino A, Yang HH, Lee MP, Difilippantonio S, Lyden DC, Steeg PS." |
| Journal name |
Clin Exp Metastasis
|
| Publication year |
2022 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231T |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 18 |
| Experiment ID |
680 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
FLOT1|TSG101
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35939247
|
| Organism |
Homo sapiens |
| Experiment description |
Metastasis suppressor NME1 in exosomes or liposomes conveys motility and migration inhibition in breast cancer model systems |
| Authors |
"Khan I, Gril B, Hoshino A, Yang HH, Lee MP, Difilippantonio S, Lyden DC, Steeg PS." |
| Journal name |
Clin Exp Metastasis
|
| Publication year |
2022 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231T |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 19 |
| Experiment ID |
681 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
FLOT1|TSG101
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35939247
|
| Organism |
Homo sapiens |
| Experiment description |
Metastasis suppressor NME1 in exosomes or liposomes conveys motility and migration inhibition in breast cancer model systems |
| Authors |
"Khan I, Gril B, Hoshino A, Yang HH, Lee MP, Difilippantonio S, Lyden DC, Steeg PS." |
| Journal name |
Clin Exp Metastasis
|
| Publication year |
2022 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-435 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 20 |
| Experiment ID |
682 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
FLOT1|TSG101
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35939247
|
| Organism |
Homo sapiens |
| Experiment description |
Metastasis suppressor NME1 in exosomes or liposomes conveys motility and migration inhibition in breast cancer model systems |
| Authors |
"Khan I, Gril B, Hoshino A, Yang HH, Lee MP, Difilippantonio S, Lyden DC, Steeg PS." |
| Journal name |
Clin Exp Metastasis
|
| Publication year |
2022 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-435 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 21 |
| Experiment ID |
909 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
TSG101
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
33579909
|
| Organism |
Homo sapiens |
| Experiment description |
Sulfisoxazole does not inhibit the secretion of small extracellular vesicles |
| Authors |
"Fonseka P, Chitti SV, Sanwlani R, Mathivanan S." |
| Journal name |
Nat Commun
|
| Publication year |
2021 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 22 |
| Experiment ID |
910 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
TSG101
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
33579909
|
| Organism |
Homo sapiens |
| Experiment description |
Sulfisoxazole does not inhibit the secretion of small extracellular vesicles |
| Authors |
"Fonseka P, Chitti SV, Sanwlani R, Mathivanan S." |
| Journal name |
Nat Commun
|
| Publication year |
2021 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 23 |
| Experiment ID |
911 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
TSG101
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
33579909
|
| Organism |
Homo sapiens |
| Experiment description |
Sulfisoxazole does not inhibit the secretion of small extracellular vesicles |
| Authors |
"Fonseka P, Chitti SV, Sanwlani R, Mathivanan S." |
| Journal name |
Nat Commun
|
| Publication year |
2021 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 24 |
| Experiment ID |
912 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
TSG101
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
33579909
|
| Organism |
Homo sapiens |
| Experiment description |
Sulfisoxazole does not inhibit the secretion of small extracellular vesicles |
| Authors |
"Fonseka P, Chitti SV, Sanwlani R, Mathivanan S." |
| Journal name |
Nat Commun
|
| Publication year |
2021 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 25 |
| Experiment ID |
913 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
TSG101
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
33579909
|
| Organism |
Homo sapiens |
| Experiment description |
Sulfisoxazole does not inhibit the secretion of small extracellular vesicles |
| Authors |
"Fonseka P, Chitti SV, Sanwlani R, Mathivanan S." |
| Journal name |
Nat Commun
|
| Publication year |
2021 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 26 |
| Experiment ID |
924 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|SDCBP|CD81|RAB35|LAMP2|FLOT1|TSG101|CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
30918259
|
| Organism |
Homo sapiens |
| Experiment description |
Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A |
| Authors |
"Im EJ, Lee CH, Moon PG, Rangaswamy GG, Lee B, Lee JM, Lee JC, Jee JG, Bae JS, Kwon TK, Kang KW, Jeong MS, Lee JE, Jung HS, Ro HJ, Jun S, Kang W, Seo SY, Cho YE, Song BJ, Baek MC." |
| Journal name |
Nat Commun
|
| Publication year |
2019 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 27 |
| Experiment ID |
925 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD9|TSG101|CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
30918259
|
| Organism |
Homo sapiens |
| Experiment description |
Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A |
| Authors |
"Im EJ, Lee CH, Moon PG, Rangaswamy GG, Lee B, Lee JM, Lee JC, Jee JG, Bae JS, Kwon TK, Kang KW, Jeong MS, Lee JE, Jung HS, Ro HJ, Jun S, Kang W, Seo SY, Cho YE, Song BJ, Baek MC." |
| Journal name |
Nat Commun
|
| Publication year |
2019 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 28 |
| Experiment ID |
926 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD9|FLOT1|TSG101|CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
30918259
|
| Organism |
Homo sapiens |
| Experiment description |
Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A |
| Authors |
"Im EJ, Lee CH, Moon PG, Rangaswamy GG, Lee B, Lee JM, Lee JC, Jee JG, Bae JS, Kwon TK, Kang KW, Jeong MS, Lee JE, Jung HS, Ro HJ, Jun S, Kang W, Seo SY, Cho YE, Song BJ, Baek MC." |
| Journal name |
Nat Commun
|
| Publication year |
2019 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 29 |
| Experiment ID |
930 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|FLOT1|TSG101|CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
30918259
|
| Organism |
Homo sapiens |
| Experiment description |
Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A |
| Authors |
"Im EJ, Lee CH, Moon PG, Rangaswamy GG, Lee B, Lee JM, Lee JC, Jee JG, Bae JS, Kwon TK, Kang KW, Jeong MS, Lee JE, Jung HS, Ro HJ, Jun S, Kang W, Seo SY, Cho YE, Song BJ, Baek MC." |
| Journal name |
Nat Commun
|
| Publication year |
2019 |
| Sample |
Breast cancer cells |
| Sample name |
MCF7 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 30 |
| Experiment ID |
931 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD9|TSG101|CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
30918259
|
| Organism |
Homo sapiens |
| Experiment description |
Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A |
| Authors |
"Im EJ, Lee CH, Moon PG, Rangaswamy GG, Lee B, Lee JM, Lee JC, Jee JG, Bae JS, Kwon TK, Kang KW, Jeong MS, Lee JE, Jung HS, Ro HJ, Jun S, Kang W, Seo SY, Cho YE, Song BJ, Baek MC." |
| Journal name |
Nat Commun
|
| Publication year |
2019 |
| Sample |
Breast cancer cells |
| Sample name |
MCF7 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 31 |
| Experiment ID |
989 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|TSG101|GAPDH
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
30089911
|
| Organism |
Homo sapiens |
| Experiment description |
Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response |
| Authors |
"Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W." |
| Journal name |
Nature
|
| Publication year |
2018 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 32 |
| Experiment ID |
1181 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
TSG101|CD81|FLOT1
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
32848136
|
| Organism |
Homo sapiens |
| Experiment description |
ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells |
| Authors |
"Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S." |
| Journal name |
Nat Commun
|
| Publication year |
2020 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 33 |
| Experiment ID |
1182 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
TSG101|CD81|FLOT1
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
32848136
|
| Organism |
Homo sapiens |
| Experiment description |
ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells |
| Authors |
"Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S." |
| Journal name |
Nat Commun
|
| Publication year |
2020 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 34 |
| Experiment ID |
1184 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
TSG101|FLOT1|CD81
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
32848136
|
| Organism |
Homo sapiens |
| Experiment description |
ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells |
| Authors |
"Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S." |
| Journal name |
Nat Commun
|
| Publication year |
2020 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 - Fraction 5 |
| Isolation/purification methods |
Differential centrifugation
OptiPrep density gradient centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 35 |
| Experiment ID |
1185 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
TSG101|FLOT1|CD81
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
32848136
|
| Organism |
Homo sapiens |
| Experiment description |
ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells |
| Authors |
"Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S." |
| Journal name |
Nat Commun
|
| Publication year |
2020 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 - Fraction 6 |
| Isolation/purification methods |
Differential centrifugation
OptiPrep density gradient centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 36 |
| Experiment ID |
1186 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
TSG101|FLOT1|CD81
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
32848136
|
| Organism |
Homo sapiens |
| Experiment description |
ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells |
| Authors |
"Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S." |
| Journal name |
Nat Commun
|
| Publication year |
2020 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 - Fraction 7 |
| Isolation/purification methods |
Differential centrifugation
OptiPrep density gradient centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 37 |
| Experiment ID |
1193 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
TSG101|FLOT1|CD81
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
32848136
|
| Organism |
Homo sapiens |
| Experiment description |
ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells |
| Authors |
"Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S." |
| Journal name |
Nat Commun
|
| Publication year |
2020 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 - Fraction 6 |
| Isolation/purification methods |
Differential centrifugation
OptiPrep density gradient centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 38 |
| Experiment ID |
1197 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
TSG101|CD81|FLOT1
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
32848136
|
| Organism |
Homo sapiens |
| Experiment description |
ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells |
| Authors |
"Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S." |
| Journal name |
Nat Commun
|
| Publication year |
2020 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 39 |
| Experiment ID |
1198 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
TSG101|CD81|FLOT1
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
32848136
|
| Organism |
Homo sapiens |
| Experiment description |
ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells |
| Authors |
"Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S." |
| Journal name |
Nat Commun
|
| Publication year |
2020 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 40 |
| Experiment ID |
1199 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
TSG101|CD81
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
32848136
|
| Organism |
Homo sapiens |
| Experiment description |
ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells |
| Authors |
"Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S." |
| Journal name |
Nat Commun
|
| Publication year |
2020 |
| Sample |
Breast cancer cells |
| Sample name |
MCF7 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 41 |
| Experiment ID |
1200 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
TSG101|CD81
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
32848136
|
| Organism |
Homo sapiens |
| Experiment description |
ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells |
| Authors |
"Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S." |
| Journal name |
Nat Commun
|
| Publication year |
2020 |
| Sample |
Breast cancer cells |
| Sample name |
MCF7 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 42 |
| Experiment ID |
1221 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
TSG101|GAPDH
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
31054213
|
| Organism |
Homo sapiens |
| Experiment description |
Phosphoproteome Profiling of Isogenic Cancer Cell-Derived Exosome Reveals HSP90 as a Potential Marker for Human Cholangiocarcinoma |
| Authors |
"Weeraphan C, Phongdara A, Chaiyawat P, Diskul-Na-Ayudthaya P, Chokchaichamnankit D, Verathamjamras C, Netsirisawan P, Yingchutrakul Y, Roytrakul S, Champattanachai V, Svasti J, Srisomsap C." |
| Journal name |
Proteomics
|
| Publication year |
2019 |
| Sample |
Cholangiocarcinoma cells |
| Sample name |
KKU-M213 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
Sucrose density gradient centrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 43 |
| Experiment ID |
1222 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
TSG101|GAPDH
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
31054213
|
| Organism |
Homo sapiens |
| Experiment description |
Phosphoproteome Profiling of Isogenic Cancer Cell-Derived Exosome Reveals HSP90 as a Potential Marker for Human Cholangiocarcinoma |
| Authors |
"Weeraphan C, Phongdara A, Chaiyawat P, Diskul-Na-Ayudthaya P, Chokchaichamnankit D, Verathamjamras C, Netsirisawan P, Yingchutrakul Y, Roytrakul S, Champattanachai V, Svasti J, Srisomsap C." |
| Journal name |
Proteomics
|
| Publication year |
2019 |
| Sample |
Cholangiocarcinoma cells |
| Sample name |
KKU-M213D5 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
Sucrose density gradient centrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 44 |
| Experiment ID |
494 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
35931686
|
| Organism |
Homo sapiens |
| Experiment description |
Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression |
| Authors |
"Varela-EirÃn M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, GarcÃa-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD." |
| Journal name |
Cell Death Dis
|
| Publication year |
2022 |
| Sample |
Chondrocytes |
| Sample name |
Osteoarthritic cartilage |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectometry |
|
|
| 45 |
| Experiment ID |
496 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
35931686
|
| Organism |
Homo sapiens |
| Experiment description |
Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression |
| Authors |
"Varela-EirÃn M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, GarcÃa-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD." |
| Journal name |
Cell Death Dis
|
| Publication year |
2022 |
| Sample |
Chondrocytes |
| Sample name |
Healthy cartilage |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectometry |
|
|
| 46 |
| Experiment ID |
497 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
35931686
|
| Organism |
Homo sapiens |
| Experiment description |
Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression |
| Authors |
"Varela-EirÃn M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, GarcÃa-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD." |
| Journal name |
Cell Death Dis
|
| Publication year |
2022 |
| Sample |
Chondrocytes |
| Sample name |
T/C-28a2 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectometry |
|
|
| 47 |
| Experiment ID |
498 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
35931686
|
| Organism |
Homo sapiens |
| Experiment description |
Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression |
| Authors |
"Varela-EirÃn M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, GarcÃa-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD." |
| Journal name |
Cell Death Dis
|
| Publication year |
2022 |
| Sample |
Chondrocytes |
| Sample name |
T/C-28a2 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectometry |
|
|
| 48 |
| Experiment ID |
1265 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD63|CD81|TSG101
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35165262
|
| Organism |
Homo sapiens |
| Experiment description |
DNAJB8 in small extracellular vesicles promotes Oxaliplatin resistance through TP53/MDR1 pathway in colon cancer |
| Authors |
"Wang Z, Li Y, Mao R, Zhang Y, Wen J, Liu Q, Liu Y, Zhang T." |
| Journal name |
Cell Death Dis
|
| Publication year |
2022 |
| Sample |
Colon cancer cells |
| Sample name |
SW480 |
| Isolation/purification methods |
Differential centrifugation
Tangential flow filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 49 |
| Experiment ID |
1266 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD63|CD81|TSG101
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35165262
|
| Organism |
Homo sapiens |
| Experiment description |
DNAJB8 in small extracellular vesicles promotes Oxaliplatin resistance through TP53/MDR1 pathway in colon cancer |
| Authors |
"Wang Z, Li Y, Mao R, Zhang Y, Wen J, Liu Q, Liu Y, Zhang T." |
| Journal name |
Cell Death Dis
|
| Publication year |
2022 |
| Sample |
Colon cancer cells |
| Sample name |
SW480 - Oxaliplatin-resistant |
| Isolation/purification methods |
Differential centrifugation
Tangential flow filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 50 |
| Experiment ID |
1267 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD63|CD81|TSG101
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35165262
|
| Organism |
Homo sapiens |
| Experiment description |
DNAJB8 in small extracellular vesicles promotes Oxaliplatin resistance through TP53/MDR1 pathway in colon cancer |
| Authors |
"Wang Z, Li Y, Mao R, Zhang Y, Wen J, Liu Q, Liu Y, Zhang T." |
| Journal name |
Cell Death Dis
|
| Publication year |
2022 |
| Sample |
Colon cancer cells |
| Sample name |
SW620 |
| Isolation/purification methods |
Differential centrifugation
Tangential flow filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 51 |
| Experiment ID |
1268 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD63|CD81|TSG101
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35165262
|
| Organism |
Homo sapiens |
| Experiment description |
DNAJB8 in small extracellular vesicles promotes Oxaliplatin resistance through TP53/MDR1 pathway in colon cancer |
| Authors |
"Wang Z, Li Y, Mao R, Zhang Y, Wen J, Liu Q, Liu Y, Zhang T." |
| Journal name |
Cell Death Dis
|
| Publication year |
2022 |
| Sample |
Colon cancer cells |
| Sample name |
SW620 - Oxaliplatin-resistant |
| Isolation/purification methods |
Differential centrifugation
Tangential flow filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 52 |
| Experiment ID |
1270 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD63|CD81|TSG101
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35165262
|
| Organism |
Homo sapiens |
| Experiment description |
DNAJB8 in small extracellular vesicles promotes Oxaliplatin resistance through TP53/MDR1 pathway in colon cancer |
| Authors |
"Wang Z, Li Y, Mao R, Zhang Y, Wen J, Liu Q, Liu Y, Zhang T." |
| Journal name |
Cell Death Dis
|
| Publication year |
2022 |
| Sample |
Colon cancer cells |
| Sample name |
COAD - Fraction 5 |
| Isolation/purification methods |
Differential centrifugation
Tangential flow filtration
Ultracentrifugation
OptiPrep density gradient centrifugation |
| Flotation density |
1.06 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 53 |
| Experiment ID |
1271 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD63|CD81|TSG101
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35165262
|
| Organism |
Homo sapiens |
| Experiment description |
DNAJB8 in small extracellular vesicles promotes Oxaliplatin resistance through TP53/MDR1 pathway in colon cancer |
| Authors |
"Wang Z, Li Y, Mao R, Zhang Y, Wen J, Liu Q, Liu Y, Zhang T." |
| Journal name |
Cell Death Dis
|
| Publication year |
2022 |
| Sample |
Colon cancer cells |
| Sample name |
COAD - Fraction 6 |
| Isolation/purification methods |
Differential centrifugation
Tangential flow filtration
Ultracentrifugation
OptiPrep density gradient centrifugation |
| Flotation density |
1.08 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 54 |
| Experiment ID |
1272 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD63|CD81|TSG101
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35165262
|
| Organism |
Homo sapiens |
| Experiment description |
DNAJB8 in small extracellular vesicles promotes Oxaliplatin resistance through TP53/MDR1 pathway in colon cancer |
| Authors |
"Wang Z, Li Y, Mao R, Zhang Y, Wen J, Liu Q, Liu Y, Zhang T." |
| Journal name |
Cell Death Dis
|
| Publication year |
2022 |
| Sample |
Colon cancer cells |
| Sample name |
COAD - Fraction 7 |
| Isolation/purification methods |
Differential centrifugation
Tangential flow filtration
Ultracentrifugation
OptiPrep density gradient centrifugation |
| Flotation density |
1.10 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 55 |
| Experiment ID |
1273 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD63|CD81|TSG101
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35165262
|
| Organism |
Homo sapiens |
| Experiment description |
DNAJB8 in small extracellular vesicles promotes Oxaliplatin resistance through TP53/MDR1 pathway in colon cancer |
| Authors |
"Wang Z, Li Y, Mao R, Zhang Y, Wen J, Liu Q, Liu Y, Zhang T." |
| Journal name |
Cell Death Dis
|
| Publication year |
2022 |
| Sample |
Colon cancer cells |
| Sample name |
COAD - Fraction 8 |
| Isolation/purification methods |
Differential centrifugation
Tangential flow filtration
Ultracentrifugation
OptiPrep density gradient centrifugation |
| Flotation density |
1.12 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 56 |
| Experiment ID |
1274 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD63|TSG101
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35165262
|
| Organism |
Homo sapiens |
| Experiment description |
DNAJB8 in small extracellular vesicles promotes Oxaliplatin resistance through TP53/MDR1 pathway in colon cancer |
| Authors |
"Wang Z, Li Y, Mao R, Zhang Y, Wen J, Liu Q, Liu Y, Zhang T." |
| Journal name |
Cell Death Dis
|
| Publication year |
2022 |
| Sample |
Colon cancer cells |
| Sample name |
COAD - Fraction 9 |
| Isolation/purification methods |
Differential centrifugation
Tangential flow filtration
Ultracentrifugation
OptiPrep density gradient centrifugation |
| Flotation density |
1.14 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 57 |
| Experiment ID |
21 |
| MISEV standards |
|
✔
EM|IEM
|
Biophysical techniques |
|
✔
Alix|TSG101|HSP70|CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
Western blotting |
|
Supporting data |
|
MS
WB
|
|
| PubMed ID |
19837982
|
| Organism |
Homo sapiens |
| Experiment description |
Proteomic and bioinformatic analysis of immunoaffinity-purified exosomes derived from the human colon tumor cell line LIM1215. |
| Authors |
"Suresh Mathivanan, Justin W.E. Lim, Bow J. Tauro, Hong Ji, Robert L. Moritz and Richard J. Simpson" |
| Journal name |
MCP
|
| Publication year |
2009 |
| Sample |
Colorectal cancer cells |
| Sample name |
LIM1215 |
| Isolation/purification methods |
Filtration
Ultracentrifugation
Sucrose density gradient |
| Flotation density |
1.10-1.12 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry [Orbitrap]
Western blotting |
|
|
| 58 |
| Experiment ID |
201 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
Alix|TSG101|HSP70|CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
22740476
|
| Organism |
Homo sapiens |
| Experiment description |
Restoration of full-length APC protein in SW480 colon cancer cells induces exosome-mediated secretion of DKK-4. |
| Authors |
"Lim JW, Mathias RA, Kapp EA, Layton MJ, Faux MC, Burgess AW, Ji H, Simpson RJ." |
| Journal name |
Electrophoresis
|
| Publication year |
2012 |
| Sample |
Colorectal cancer cells |
| Sample name |
SW480 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
| 59 |
| Experiment ID |
207 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
TSG101|HSP70|FLOT1
|
Enriched markers |
|
✔
VDAC
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
23161513
|
| Organism |
Homo sapiens |
| Experiment description |
Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. |
| Authors |
"Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ." |
| Journal name |
Mol Cell Proteomics
|
| Publication year |
2012 |
| Sample |
Colorectal cancer cells |
| Sample name |
DKO-1 |
| Isolation/purification methods |
Differential centrifugation
Filtration |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
| 60 |
| Experiment ID |
208 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
TSG101|HSP70|FLOT1
|
Enriched markers |
|
✔
VDAC
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
23161513
|
| Organism |
Homo sapiens |
| Experiment description |
Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. |
| Authors |
"Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ." |
| Journal name |
Mol Cell Proteomics
|
| Publication year |
2012 |
| Sample |
Colorectal cancer cells |
| Sample name |
Dks-8 |
| Isolation/purification methods |
Differential centrifugation
Filtration |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
| 61 |
| Experiment ID |
209 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
TSG101|HSP70|FLOT1
|
Enriched markers |
|
✔
VDAC
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
23161513
|
| Organism |
Homo sapiens |
| Experiment description |
Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. |
| Authors |
"Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ." |
| Journal name |
Mol Cell Proteomics
|
| Publication year |
2012 |
| Sample |
Colorectal cancer cells |
| Sample name |
DLD-1 |
| Isolation/purification methods |
Differential centrifugation
Filtration |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
| 62 |
| Experiment ID |
223 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
Alix|TSG101|CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
25330373
|
| Organism |
Homo sapiens |
| Experiment description |
Deep sequencing of RNA from three different extracellular vesicle (EV) subtypes released from the human LIM1863 colon cancer cell line uncovers distinct miRNA-enrichment signatures |
| Authors |
"Ji H, Chen M, Greening DW, He W, Rai A, Zhang W, Simpson RJ" |
| Journal name |
PLoS One
|
| Publication year |
2014 |
| Sample |
Colorectal cancer cells |
| Sample name |
LIM1863 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation
Immunobeads (EpCAM)
Immunobeads (A33) |
| Flotation density |
-
|
| Molecules identified in the study |
miRNA
Protein |
| Methods used in the study |
RT-PCR
Western blotting |
|
|
| 63 |
| Experiment ID |
271 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
TSG101|Alix
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
25907253
|
| Organism |
Homo sapiens |
| Experiment description |
Rapid and comprehensive 'shotgun' lipidome profiling of colorectal cancer cell derived exosomes. |
| Authors |
"Lydic TA, Townsend S, Adda CG, Collins C, Mathivanan S, Reid GE." |
| Journal name |
Methods
|
| Publication year |
2015 |
| Sample |
Colorectal cancer cells |
| Sample name |
LIM1215 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Lipids
Protein |
| Methods used in the study |
Mass spectrometry
Western blotting |
|
|
| 64 |
| Experiment ID |
282 |
| MISEV standards |
|
✔
CEM
|
Biophysical techniques |
|
✔
Alix|TSG101|CD63|CD81|EpCAM
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
DLS
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
25890246
|
| Organism |
Homo sapiens |
| Experiment description |
Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct. |
| Authors |
"Xu R, Greening DW, Rai A, Ji H, Simpson RJ." |
| Journal name |
Methods
|
| Publication year |
2015 |
| Sample |
Colorectal cancer cells |
| Sample name |
LIM1863 - Ultracentrifugation - Rep 1 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry
Western blotting |
|
|
| 65 |
| Experiment ID |
283 |
| MISEV standards |
|
✔
CEM
|
Biophysical techniques |
|
✔
Alix|TSG101|CD63|CD81|EpCAM
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
DLS
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
25890246
|
| Organism |
Homo sapiens |
| Experiment description |
Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct. |
| Authors |
"Xu R, Greening DW, Rai A, Ji H, Simpson RJ." |
| Journal name |
Methods
|
| Publication year |
2015 |
| Sample |
Colorectal cancer cells |
| Sample name |
LIM1863 - Ultracentrifugation - Rep 2 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry
Western blotting |
|
|
| 66 |
| Experiment ID |
285 |
| MISEV standards |
|
✔
CEM
|
Biophysical techniques |
|
✔
Alix|TSG101|CD63|CD81|EpCAM
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
DLS
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
Mass spectrometry
|
| PubMed ID |
25890246
|
| Organism |
Homo sapiens |
| Experiment description |
Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct. |
| Authors |
"Xu R, Greening DW, Rai A, Ji H, Simpson RJ." |
| Journal name |
Methods
|
| Publication year |
2015 |
| Sample |
Colorectal cancer cells |
| Sample name |
LIM1863 - Sequential centrifugal ultrafiltration - Rep 1 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Sequential centrifugal ultrafiltration
Centrifugal concentration |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry
Western blotting |
|
|
| 67 |
| Experiment ID |
286 |
| MISEV standards |
|
✔
CEM
|
Biophysical techniques |
|
✔
Alix|TSG101|CD63|CD81|EpCAM
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
DLS
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
Mass spectrometry
|
| PubMed ID |
25890246
|
| Organism |
Homo sapiens |
| Experiment description |
Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct. |
| Authors |
"Xu R, Greening DW, Rai A, Ji H, Simpson RJ." |
| Journal name |
Methods
|
| Publication year |
2015 |
| Sample |
Colorectal cancer cells |
| Sample name |
LIM1863 - Sequential centrifugal ultrafiltration - Rep 2 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Sequential centrifugal ultrafiltration
Centrifugal concentration |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry
Western blotting |
|
|
| 68 |
| Experiment ID |
284 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
TSG101
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
25890246
|
| Organism |
Homo sapiens |
| Experiment description |
Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct. |
| Authors |
"Xu R, Greening DW, Rai A, Ji H, Simpson RJ." |
| Journal name |
Methods
|
| Publication year |
2015 |
| Sample |
Colorectal cancer cells |
| Sample name |
LIM1863 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient
Centrifugal concentration |
| Flotation density |
1.10-1.11 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 69 |
| Experiment ID |
314 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
TSG101|CD81
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
33368224
|
| Organism |
Homo sapiens |
| Experiment description |
Colorectal cancer prompted adipose tissue browning and cancer cachexia through transferring exosomal miR-146b-5p |
| Authors |
"Di W, Zhang W, Zhu B, Li X, Tang Q, Zhou Y. " |
| Journal name |
J Cell Physiol
|
| Publication year |
2020 |
| Sample |
Colorectal cancer cells |
| Sample name |
HCT116 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
mRNA |
| Methods used in the study |
Western blotting
RT-PCT |
|
|
| 70 |
| Experiment ID |
324 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
TSG101
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
32438511
|
| Organism |
Homo sapiens |
| Experiment description |
Exosomes Derived from the Human Primary Colorectal Cancer Cell Line SW480 Orchestrate Fibroblast-Led Cancer Invasion |
| Authors |
"Rai A, Greening DW, Xu R, Suwakulsiri W, Simpson RJ." |
| Journal name |
Proteomics
|
| Publication year |
2020 |
| Sample |
Colorectal cancer cells |
| Sample name |
SW480 - Fraction 6 |
| Isolation/purification methods |
Differential centrifugation
Filtration
OptiPrep density gradient centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 71 |
| Experiment ID |
325 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
TSG101
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
32438511
|
| Organism |
Homo sapiens |
| Experiment description |
Exosomes Derived from the Human Primary Colorectal Cancer Cell Line SW480 Orchestrate Fibroblast-Led Cancer Invasion |
| Authors |
"Rai A, Greening DW, Xu R, Suwakulsiri W, Simpson RJ." |
| Journal name |
Proteomics
|
| Publication year |
2020 |
| Sample |
Colorectal cancer cells |
| Sample name |
SW480 - Fraction 7 |
| Isolation/purification methods |
Differential centrifugation
Filtration
OptiPrep density gradient centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 72 |
| Experiment ID |
326 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
TSG101|CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
32438511
|
| Organism |
Homo sapiens |
| Experiment description |
Exosomes Derived from the Human Primary Colorectal Cancer Cell Line SW480 Orchestrate Fibroblast-Led Cancer Invasion |
| Authors |
"Rai A, Greening DW, Xu R, Suwakulsiri W, Simpson RJ." |
| Journal name |
Proteomics
|
| Publication year |
2020 |
| Sample |
Colorectal cancer cells |
| Sample name |
SW480 - Fraction 6 |
| Isolation/purification methods |
Differential centrifugation
Filtration
OptiPrep density gradient centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 73 |
| Experiment ID |
327 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
TSG101|CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
32438511
|
| Organism |
Homo sapiens |
| Experiment description |
Exosomes Derived from the Human Primary Colorectal Cancer Cell Line SW480 Orchestrate Fibroblast-Led Cancer Invasion |
| Authors |
"Rai A, Greening DW, Xu R, Suwakulsiri W, Simpson RJ." |
| Journal name |
Proteomics
|
| Publication year |
2020 |
| Sample |
Colorectal cancer cells |
| Sample name |
SW480 - Fraction 7 |
| Isolation/purification methods |
Differential centrifugation
Filtration
OptiPrep density gradient centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 74 |
| Experiment ID |
336 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|CD81|EPCAM|TSG101
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
29472616
|
| Organism |
Homo sapiens |
| Experiment description |
Mutant p53 cancers reprogram macrophages to tumor supporting macrophages via exosomal miR-1246 |
| Authors |
"Cooks T, Pateras IS, Jenkins LM, Patel KM, Robles AI, Morris J, Forshew T, Appella E, Gorgoulis VG, Harris CC." |
| Journal name |
Nat Commun
|
| Publication year |
2018 |
| Sample |
Colorectal cancer cells |
| Sample name |
HCT116 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry
Western blotting |
|
|
| 75 |
| Experiment ID |
337 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD9|CD81|EPCAM|TSG101
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
29472616
|
| Organism |
Homo sapiens |
| Experiment description |
Mutant p53 cancers reprogram macrophages to tumor supporting macrophages via exosomal miR-1246 |
| Authors |
"Cooks T, Pateras IS, Jenkins LM, Patel KM, Robles AI, Morris J, Forshew T, Appella E, Gorgoulis VG, Harris CC." |
| Journal name |
Nat Commun
|
| Publication year |
2018 |
| Sample |
Colorectal cancer cells |
| Sample name |
HCT116 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry
Western blotting |
|
|
| 76 |
| Experiment ID |
338 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
TSG101|CD9
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
29472616
|
| Organism |
Homo sapiens |
| Experiment description |
Mutant p53 cancers reprogram macrophages to tumor supporting macrophages via exosomal miR-1246 |
| Authors |
"Cooks T, Pateras IS, Jenkins LM, Patel KM, Robles AI, Morris J, Forshew T, Appella E, Gorgoulis VG, Harris CC." |
| Journal name |
Nat Commun
|
| Publication year |
2018 |
| Sample |
Colorectal cancer cells |
| Sample name |
HCT116 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 77 |
| Experiment ID |
339 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
TSG101|CD9
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
29472616
|
| Organism |
Homo sapiens |
| Experiment description |
Mutant p53 cancers reprogram macrophages to tumor supporting macrophages via exosomal miR-1246 |
| Authors |
"Cooks T, Pateras IS, Jenkins LM, Patel KM, Robles AI, Morris J, Forshew T, Appella E, Gorgoulis VG, Harris CC." |
| Journal name |
Nat Commun
|
| Publication year |
2018 |
| Sample |
Colorectal cancer cells |
| Sample name |
HCT116 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
miRNA |
| Methods used in the study |
Western blotting
miR-chip array |
|
|
| 78 |
| Experiment ID |
340 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
TSG101|CD9
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
29472616
|
| Organism |
Homo sapiens |
| Experiment description |
Mutant p53 cancers reprogram macrophages to tumor supporting macrophages via exosomal miR-1246 |
| Authors |
"Cooks T, Pateras IS, Jenkins LM, Patel KM, Robles AI, Morris J, Forshew T, Appella E, Gorgoulis VG, Harris CC." |
| Journal name |
Nat Commun
|
| Publication year |
2018 |
| Sample |
Colorectal cancer cells |
| Sample name |
HCT116 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
miRNA |
| Methods used in the study |
Western blotting
miR-chip array |
|
|
| 79 |
| Experiment ID |
341 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
TSG101|CD9
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
29472616
|
| Organism |
Homo sapiens |
| Experiment description |
Mutant p53 cancers reprogram macrophages to tumor supporting macrophages via exosomal miR-1246 |
| Authors |
"Cooks T, Pateras IS, Jenkins LM, Patel KM, Robles AI, Morris J, Forshew T, Appella E, Gorgoulis VG, Harris CC." |
| Journal name |
Nat Commun
|
| Publication year |
2018 |
| Sample |
Colorectal cancer cells |
| Sample name |
HCT116 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
miRNA |
| Methods used in the study |
Western blotting
miR-chip array |
|
|
| 80 |
| Experiment ID |
1203 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34887515
|
| Organism |
Homo sapiens |
| Experiment description |
Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets |
| Authors |
"Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Colorectal cancer cells |
| Sample name |
DiFi |
| Isolation/purification methods |
Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
miRNA |
| Methods used in the study |
Western blotting
Mass spectrometry
RNA sequencing |
|
|
| 81 |
| Experiment ID |
1340 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD9|TSG101|CD63|FLOT1|Alix
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36068649
|
| Organism |
Homo sapiens |
| Experiment description |
Extracellular vesicles from colorectal cancer cells promote metastasis via the NOD1 signalling pathway |
| Authors |
"Wei X, Ye J, Pei Y, Wang C, Yang H, Tian J, Si G, Ma Y, Wang K, Liu G" |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Colorectal cancer cells |
| Sample name |
HT29 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectometry |
|
|
| 82 |
| Experiment ID |
1341 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD9|TSG101|CD63|FLOT1|Alix
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36068649
|
| Organism |
Homo sapiens |
| Experiment description |
Extracellular vesicles from colorectal cancer cells promote metastasis via the NOD1 signalling pathway |
| Authors |
"Wei X, Ye J, Pei Y, Wang C, Yang H, Tian J, Si G, Ma Y, Wang K, Liu G" |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Colorectal cancer cells |
| Sample name |
HCT116 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 83 |
| Experiment ID |
1361 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
TSG101|CD63|FLOT1|LAMP1|CD24|EPCAM|Alix
|
Enriched markers |
|
✔
HSP90AA1
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36303008
|
| Organism |
Homo sapiens |
| Experiment description |
A hydrogel-based mechanical metamaterial for the interferometric profiling of extracellular vesicles in patient samples |
| Authors |
"Zhao H, Pan S, Natalia A, Wu X, Ong CJ, Teo MCC, So JBY, Shao H." |
| Journal name |
Nat Biomed Eng
|
| Publication year |
2023 |
| Sample |
Colorectal cancer cells |
| Sample name |
HCT116 |
| Isolation/purification methods |
Filtration
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
MORPH
ELISA |
|
|
| 84 |
| Experiment ID |
117 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✘
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
21505438
|
| Organism |
Homo sapiens |
| Experiment description |
Unidirectional transfer of microRNA-loaded exosomes from T cells to antigen-presenting cells. |
| Authors |
"Mittelbrunn M, Gutierrez-Vázquez C, Villarroya-Beltri C, González S, Sánchez-Cabo F, González MÃÂ, Bernad A, Sánchez-Madrid F." |
| Journal name |
NATCOM
|
| Publication year |
2011 |
| Sample |
Dendritic cells |
| Sample name |
Dendritic cells |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
miRNA |
| Methods used in the study |
Mass spectrometry
Microarray |
|
|
| 85 |
| Experiment ID |
395 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
TSG101|CD63|GAPDH
|
Enriched markers |
|
✔
GOLGA2
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
33523942
|
| Organism |
Homo sapiens |
| Experiment description |
Exosomal delivery of NF-κB inhibitor delays LPS-induced preterm birth and modulates fetal immune cell profile in mouse models |
| Authors |
"Sheller-Miller S, Radnaa E, Yoo JK, Kim E, Choi K, Kim Y, Kim YN, Richardson L, Choi C, Menon R." |
| Journal name |
Sci Adv
|
| Publication year |
2021 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T |
| Isolation/purification methods |
Centrifugation
Filtration
Size exclusion chromatography |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 86 |
| Experiment ID |
396 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
TSG101|CD63|GAPDH
|
Enriched markers |
|
✔
GOLGA2
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
33523942
|
| Organism |
Homo sapiens |
| Experiment description |
Exosomal delivery of NF-κB inhibitor delays LPS-induced preterm birth and modulates fetal immune cell profile in mouse models |
| Authors |
"Sheller-Miller S, Radnaa E, Yoo JK, Kim E, Choi K, Kim Y, Kim YN, Richardson L, Choi C, Menon R." |
| Journal name |
Sci Adv
|
| Publication year |
2021 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T |
| Isolation/purification methods |
Centrifugation
Filtration
Size exclusion chromatography |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 87 |
| Experiment ID |
407 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|CD63|CD81|SDCBP|TSG101|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|RAB35
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
mRNA |
| Methods used in the study |
RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry |
|
|
| 88 |
| Experiment ID |
407 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|CD63|CD81|SDCBP|TSG101|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|RAB35
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
mRNA |
| Methods used in the study |
RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry |
|
|
| 89 |
| Experiment ID |
419 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T - Exo-rich fractions 7-10 pooled |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 90 |
| Experiment ID |
419 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T - Exo-rich fractions 7-10 pooled |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 91 |
| Experiment ID |
420 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T - Exo-rich fractions 1-6 pooled |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
| 92 |
| Experiment ID |
420 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T - Exo-rich fractions 1-6 pooled |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
| 93 |
| Experiment ID |
1145 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD63|CD81|TSG101
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34545097
|
| Organism |
Homo sapiens |
| Experiment description |
Exosome-mediated stable epigenetic repression of HIV-1 |
| Authors |
"Shrivastava S, Ray RM, Holguin L, Echavarria L, Grepo N, Scott TA, Burnett J, Morris KV." |
| Journal name |
Nat Commun
|
| Publication year |
2021 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation
Filtration |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 94 |
| Experiment ID |
1146 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD63|CD81|TSG101
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34545097
|
| Organism |
Homo sapiens |
| Experiment description |
Exosome-mediated stable epigenetic repression of HIV-1 |
| Authors |
"Shrivastava S, Ray RM, Holguin L, Echavarria L, Grepo N, Scott TA, Burnett J, Morris KV." |
| Journal name |
Nat Commun
|
| Publication year |
2021 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation
Filtration |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
mRNA |
| Methods used in the study |
Western blotting
RT-qPCR |
|
|
| 95 |
| Experiment ID |
1147 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD63|CD81|TSG101
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34545097
|
| Organism |
Homo sapiens |
| Experiment description |
Exosome-mediated stable epigenetic repression of HIV-1 |
| Authors |
"Shrivastava S, Ray RM, Holguin L, Echavarria L, Grepo N, Scott TA, Burnett J, Morris KV." |
| Journal name |
Nat Commun
|
| Publication year |
2021 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation
Filtration |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
mRNA |
| Methods used in the study |
Western blotting
RT-qPCR |
|
|
| 96 |
| Experiment ID |
1275 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD9|TSG101|FLOT1|FLOT2|RAB35|RAB5A|GAPDH|TFRC|CD63|Alix
|
Enriched markers |
|
✔
PCNA|CANX|GAPDH|HSP90AA1|HSP90B1
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36706192
|
| Organism |
Homo sapiens |
| Experiment description |
Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles |
| Authors |
"Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q." |
| Journal name |
Sci Adv
|
| Publication year |
2023 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 97 |
| Experiment ID |
1275 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD9|TSG101|FLOT1|FLOT2|RAB35|RAB5A|GAPDH|TFRC|CD63|Alix
|
Enriched markers |
|
✔
PCNA|CANX|GAPDH|HSP90AA1|HSP90B1
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
36706192
|
| Organism |
Homo sapiens |
| Experiment description |
Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles |
| Authors |
"Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q." |
| Journal name |
Sci Adv
|
| Publication year |
2023 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 98 |
| Experiment ID |
1278 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD9|TSG101
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36706192
|
| Organism |
Homo sapiens |
| Experiment description |
Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles |
| Authors |
"Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q." |
| Journal name |
Sci Adv
|
| Publication year |
2023 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 99 |
| Experiment ID |
1280 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
TSG101|CD9|FLOT1|FLOT2|RAB35|RAB5A|GAPDH|TFRC|CD63|Alix
|
Enriched markers |
|
✔
PCNA|CANX|GAPDH|HSP90AA1|HSP90B1
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36706192
|
| Organism |
Homo sapiens |
| Experiment description |
Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles |
| Authors |
"Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q." |
| Journal name |
Sci Adv
|
| Publication year |
2023 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T - Fraction 5 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation |
| Flotation density |
1.151 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 100 |
| Experiment ID |
1280 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
TSG101|CD9|FLOT1|FLOT2|RAB35|RAB5A|GAPDH|TFRC|CD63|Alix
|
Enriched markers |
|
✔
PCNA|CANX|GAPDH|HSP90AA1|HSP90B1
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
36706192
|
| Organism |
Homo sapiens |
| Experiment description |
Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles |
| Authors |
"Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q." |
| Journal name |
Sci Adv
|
| Publication year |
2023 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T - Fraction 5 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation |
| Flotation density |
1.151 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 101 |
| Experiment ID |
1284 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
TSG101|CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36706192
|
| Organism |
Homo sapiens |
| Experiment description |
Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles |
| Authors |
"Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q." |
| Journal name |
Sci Adv
|
| Publication year |
2023 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 102 |
| Experiment ID |
1285 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
TSG101|CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36706192
|
| Organism |
Homo sapiens |
| Experiment description |
Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles |
| Authors |
"Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q." |
| Journal name |
Sci Adv
|
| Publication year |
2023 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 103 |
| Experiment ID |
1289 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
TSG101|CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36706192
|
| Organism |
Homo sapiens |
| Experiment description |
Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles |
| Authors |
"Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q." |
| Journal name |
Sci Adv
|
| Publication year |
2023 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 104 |
| Experiment ID |
1290 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
TSG101|CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36706192
|
| Organism |
Homo sapiens |
| Experiment description |
Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles |
| Authors |
"Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q." |
| Journal name |
Sci Adv
|
| Publication year |
2023 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 105 |
| Experiment ID |
1337 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
TSG101|CD9|CD81
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35917363
|
| Organism |
Homo sapiens |
| Experiment description |
CD9 mediates the uptake of extracellular vesicles from cancer-associated fibroblasts that promote pancreatic cancer cell aggressiveness |
| Authors |
"Nigri J, Leca J, Tubiana SS, Finetti P, Guillaumond F, Martinez S, Lac S, Iovanna JL, Audebert S, Camoin L, Vasseur S, Bertucci F, Tomasini R." |
| Journal name |
Sci Signal
|
| Publication year |
2022 |
| Sample |
Fibroblasts |
| Sample name |
CAF - Pancreatic ductal adenocarcinoma |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectometry |
|
|
| 106 |
| Experiment ID |
1338 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
TSG101|CD9|CD81
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35917363
|
| Organism |
Homo sapiens |
| Experiment description |
CD9 mediates the uptake of extracellular vesicles from cancer-associated fibroblasts that promote pancreatic cancer cell aggressiveness |
| Authors |
"Nigri J, Leca J, Tubiana SS, Finetti P, Guillaumond F, Martinez S, Lac S, Iovanna JL, Audebert S, Camoin L, Vasseur S, Bertucci F, Tomasini R." |
| Journal name |
Sci Signal
|
| Publication year |
2022 |
| Sample |
Fibroblasts |
| Sample name |
CAF - Pancreatic ductal adenocarcinoma |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectometry |
|
|
| 107 |
| Experiment ID |
405 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|CD63|CD81|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Foreskin fibroblasts |
| Sample name |
BJ |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
mRNA |
| Methods used in the study |
RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry |
|
|
| 108 |
| Experiment ID |
363 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
|
Enriched markers |
|
✔
DCLK1
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
33991177
|
| Organism |
Homo sapiens |
| Experiment description |
Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells |
| Authors |
"Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." |
| Journal name |
Proteomics
|
| Publication year |
2021 |
| Sample |
Gastric cancer cells |
| Sample name |
MKN1 - 100K pellet |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 109 |
| Experiment ID |
363 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
|
Enriched markers |
|
✔
DCLK1
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
33991177
|
| Organism |
Homo sapiens |
| Experiment description |
Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells |
| Authors |
"Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." |
| Journal name |
Proteomics
|
| Publication year |
2021 |
| Sample |
Gastric cancer cells |
| Sample name |
MKN1 - 100K pellet |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 110 |
| Experiment ID |
364 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
|
Enriched markers |
|
✔
DCLK1
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
33991177
|
| Organism |
Homo sapiens |
| Experiment description |
Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells |
| Authors |
"Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." |
| Journal name |
Proteomics
|
| Publication year |
2021 |
| Sample |
Gastric cancer cells |
| Sample name |
MKN1 - 100K pellet |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 111 |
| Experiment ID |
364 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
|
Enriched markers |
|
✔
DCLK1
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
33991177
|
| Organism |
Homo sapiens |
| Experiment description |
Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells |
| Authors |
"Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." |
| Journal name |
Proteomics
|
| Publication year |
2021 |
| Sample |
Gastric cancer cells |
| Sample name |
MKN1 - 100K pellet |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 112 |
| Experiment ID |
365 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
|
Enriched markers |
|
✔
DCLK1
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
33991177
|
| Organism |
Homo sapiens |
| Experiment description |
Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells |
| Authors |
"Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." |
| Journal name |
Proteomics
|
| Publication year |
2021 |
| Sample |
Gastric cancer cells |
| Sample name |
MKN1 - 100K pellet |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 113 |
| Experiment ID |
365 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
|
Enriched markers |
|
✔
DCLK1
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
33991177
|
| Organism |
Homo sapiens |
| Experiment description |
Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells |
| Authors |
"Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." |
| Journal name |
Proteomics
|
| Publication year |
2021 |
| Sample |
Gastric cancer cells |
| Sample name |
MKN1 - 100K pellet |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 114 |
| Experiment ID |
272 |
| MISEV standards |
|
✔
AFM
|
Biophysical techniques |
|
✔
TSG101|CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
DLS
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
25498500
|
| Organism |
Homo sapiens |
| Experiment description |
Effective internalization of U251-MG-secreted exosomes into cancer cells and characterization of their lipid components. |
| Authors |
"Toda Y, Takata K, Nakagawa Y, Kawakami H, Fujioka S, Kobayashi K, Hattori Y, Kitamura Y, Akaji K, Ashihara E." |
| Journal name |
Biochem Biophys Res Commun
|
| Publication year |
2015 |
| Sample |
Glioblastoma cells |
| Sample name |
U251 |
| Isolation/purification methods |
Differential centrifugation
Ultracenrifugation
Sucrose density gradient |
| Flotation density |
1.16 g/mL
|
| Molecules identified in the study |
Lipids
Protein |
| Methods used in the study |
Thin layer chromatography
Western blotting |
|
|
| 115 |
| Experiment ID |
515 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD63|TSG101
|
Enriched markers |
|
✔
GOLGA2
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36103526
|
| Organism |
Homo sapiens |
| Experiment description |
Exosome-mediated delivery of Cas9 ribonucleoprotein complexes for tissue-specific gene therapy of liver diseases |
| Authors |
"Wan T, Zhong J, Pan Q, Zhou T, Ping Y, Liu X." |
| Journal name |
Sci Adv
|
| Publication year |
2022 |
| Sample |
Hepatic stellate cells |
| Sample name |
LX-2 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 116 |
| Experiment ID |
234 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
TSG101|Alix|HSC70|GAPDH
|
Enriched markers |
|
✔
HSP90B1
|
Negative markers |
|
✔
qNano
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
26054723
|
| Organism |
Homo sapiens |
| Experiment description |
Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs |
| Authors |
"He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N" |
| Journal name |
Carcinogenesis
|
| Publication year |
2015 |
| Sample |
Hepatocellular carcinoma cells |
| Sample name |
HKCI-C3 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient |
| Flotation density |
1.13-1.19 g/mL
|
| Molecules identified in the study |
Protein
RNA |
| Methods used in the study |
Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing |
|
|
| 117 |
| Experiment ID |
235 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
TSG101|Alix|HSC70|GAPDH
|
Enriched markers |
|
✔
HSP90B1
|
Negative markers |
|
✔
qNano
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
26054723
|
| Organism |
Homo sapiens |
| Experiment description |
Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs |
| Authors |
"He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N" |
| Journal name |
Carcinogenesis
|
| Publication year |
2015 |
| Sample |
Hepatocellular carcinoma cells |
| Sample name |
HKCI-8 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient |
| Flotation density |
1.13-1.19 g/mL
|
| Molecules identified in the study |
Protein
RNA |
| Methods used in the study |
Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing |
|
|
| 118 |
| Experiment ID |
236 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
TSG101|Alix|HSC70|GAPDH
|
Enriched markers |
|
✔
HSP90B1
|
Negative markers |
|
✔
qNano
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
26054723
|
| Organism |
Homo sapiens |
| Experiment description |
Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs |
| Authors |
"He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N" |
| Journal name |
Carcinogenesis
|
| Publication year |
2015 |
| Sample |
Hepatocellular carcinoma cells |
| Sample name |
MHCC97L |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient |
| Flotation density |
1.13-1.19 g/mL
|
| Molecules identified in the study |
Protein
RNA |
| Methods used in the study |
Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing |
|
|
| 119 |
| Experiment ID |
237 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
TSG101|Alix|HSC70|GAPDH
|
Enriched markers |
|
✔
HSP90B1
|
Negative markers |
|
✔
qNano
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
26054723
|
| Organism |
Homo sapiens |
| Experiment description |
Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs |
| Authors |
"He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N" |
| Journal name |
Carcinogenesis
|
| Publication year |
2015 |
| Sample |
Hepatocytes |
| Sample name |
MIHA |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient |
| Flotation density |
1.13-1.19 g/mL
|
| Molecules identified in the study |
Protein
RNA |
| Methods used in the study |
Western blotting
Mass spectrometry
RNA Sequencing |
|
|
| 120 |
| Experiment ID |
313 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
TSG101|CD81
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
33368224
|
| Organism |
Homo sapiens |
| Experiment description |
Colorectal cancer prompted adipose tissue browning and cancer cachexia through transferring exosomal miR-146b-5p |
| Authors |
"Di W, Zhang W, Zhu B, Li X, Tang Q, Zhou Y. " |
| Journal name |
J Cell Physiol
|
| Publication year |
2020 |
| Sample |
Intestinal epithelial cells |
| Sample name |
FHs 74 Int |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
mRNA |
| Methods used in the study |
Western blotting
RT-PCT |
|
|
| 121 |
| Experiment ID |
846 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
TSG101
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35333565
|
| Organism |
Homo sapiens |
| Experiment description |
LAMP2A regulates the loading of proteins into exosomes |
| Authors |
"Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" |
| Journal name |
Sci Adv
|
| Publication year |
2022 |
| Sample |
Kidney cancer cells |
| Sample name |
769-P |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 122 |
| Experiment ID |
847 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
TSG101
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35333565
|
| Organism |
Homo sapiens |
| Experiment description |
LAMP2A regulates the loading of proteins into exosomes |
| Authors |
"Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" |
| Journal name |
Sci Adv
|
| Publication year |
2022 |
| Sample |
Kidney cancer cells |
| Sample name |
769-P |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 123 |
| Experiment ID |
266 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
TSG101|Alix
|
Enriched markers |
|
✔
CANX|HSP90B1
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
25265333
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomic Analysis of Exosome Protein Content Changes Induced by Hepatitis B Virus in Huh-7 Cells Using SILAC Labeling and LC-MS/MS |
| Authors |
"Zhao X, Wu Y, Duan J, Ma Y, Shen Z, Wei L, Cui X, Zhang J, Xie Y, Liu J" |
| Journal name |
J Proteome Res
|
| Publication year |
2014 |
| Sample |
Liver cancer cells |
| Sample name |
Huh-7 - Wild type |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 124 |
| Experiment ID |
267 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
TSG101|Alix
|
Enriched markers |
|
✔
CANX|HSP90B1
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
25265333
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomic Analysis of Exosome Protein Content Changes Induced by Hepatitis B Virus in Huh-7 Cells Using SILAC Labeling and LC-MS/MS |
| Authors |
"Zhao X, Wu Y, Duan J, Ma Y, Shen Z, Wei L, Cui X, Zhang J, Xie Y, Liu J" |
| Journal name |
J Proteome Res
|
| Publication year |
2014 |
| Sample |
Liver cancer cells |
| Sample name |
Huh-7 - Transfected with HBx plasmids |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 125 |
| Experiment ID |
268 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
TSG101|Alix
|
Enriched markers |
|
✔
CANX|HSP90B1
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
25265333
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomic Analysis of Exosome Protein Content Changes Induced by Hepatitis B Virus in Huh-7 Cells Using SILAC Labeling and LC-MS/MS |
| Authors |
"Zhao X, Wu Y, Duan J, Ma Y, Shen Z, Wei L, Cui X, Zhang J, Xie Y, Liu J" |
| Journal name |
J Proteome Res
|
| Publication year |
2014 |
| Sample |
Liver cancer cells |
| Sample name |
Huh-7 - Transfected with HBx-null HBV replicon plasmids |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 126 |
| Experiment ID |
691 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
HSP70|CD63|TSG101|CD9|CD81
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Western blotting
|
| PubMed ID |
29335551
|
| Organism |
Homo sapiens |
| Experiment description |
Tumor-derived exosomal miR-1247-3p induces cancer-associated fibroblast activation to foster lung metastasis of liver cancer |
| Authors |
"Fang T, Lv H, Lv G, Li T, Wang C, Han Q, Yu L, Su B, Guo L, Huang S, Cao D, Tang L, Tang S, Wu M, Yang W, Wang H." |
| Journal name |
Nat Commun
|
| Publication year |
2018 |
| Sample |
Liver cancer cells |
| Sample name |
CSQT-2 |
| Isolation/purification methods |
Filtration
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
miRNA |
| Methods used in the study |
Western blotting
Microarray
qRT-PCR |
|
|
| 127 |
| Experiment ID |
692 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
HSP70|CD63|TSG101|CD9|CD81
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Western blotting
|
| PubMed ID |
29335551
|
| Organism |
Homo sapiens |
| Experiment description |
Tumor-derived exosomal miR-1247-3p induces cancer-associated fibroblast activation to foster lung metastasis of liver cancer |
| Authors |
"Fang T, Lv H, Lv G, Li T, Wang C, Han Q, Yu L, Su B, Guo L, Huang S, Cao D, Tang L, Tang S, Wu M, Yang W, Wang H." |
| Journal name |
Nat Commun
|
| Publication year |
2018 |
| Sample |
Liver cancer cells |
| Sample name |
HCC-LM3 |
| Isolation/purification methods |
Filtration
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
miRNA |
| Methods used in the study |
Western blotting
Microarray
qRT-PCR |
|
|
| 128 |
| Experiment ID |
693 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
HSP70|CD63|TSG101|CD9|CD81
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Western blotting
|
| PubMed ID |
29335551
|
| Organism |
Homo sapiens |
| Experiment description |
Tumor-derived exosomal miR-1247-3p induces cancer-associated fibroblast activation to foster lung metastasis of liver cancer |
| Authors |
"Fang T, Lv H, Lv G, Li T, Wang C, Han Q, Yu L, Su B, Guo L, Huang S, Cao D, Tang L, Tang S, Wu M, Yang W, Wang H." |
| Journal name |
Nat Commun
|
| Publication year |
2018 |
| Sample |
Liver cancer cells |
| Sample name |
HepG2 |
| Isolation/purification methods |
Filtration
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
miRNA |
| Methods used in the study |
Western blotting
Microarray
qRT-PCR |
|
|
| 129 |
| Experiment ID |
694 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
HSP70|CD63|TSG101|CD9|CD81
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Western blotting
|
| PubMed ID |
29335551
|
| Organism |
Homo sapiens |
| Experiment description |
Tumor-derived exosomal miR-1247-3p induces cancer-associated fibroblast activation to foster lung metastasis of liver cancer |
| Authors |
"Fang T, Lv H, Lv G, Li T, Wang C, Han Q, Yu L, Su B, Guo L, Huang S, Cao D, Tang L, Tang S, Wu M, Yang W, Wang H." |
| Journal name |
Nat Commun
|
| Publication year |
2018 |
| Sample |
Liver cancer cells |
| Sample name |
MHCC-97L |
| Isolation/purification methods |
Filtration
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
miRNA |
| Methods used in the study |
Western blotting
Microarray
qRT-PCR |
|
|
| 130 |
| Experiment ID |
1308 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
TSG101|CD63|CD81|Alix
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
32082513
|
| Organism |
Homo sapiens |
| Experiment description |
Hepatitis A virus structural protein pX interacts with ALIX and promotes the secretion of virions and foreign proteins through exosome-like vesicles |
| Authors |
"Jiang W, Ma P, Deng L, Liu Z, Wang X, Liu X, Long G." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2020 |
| Sample |
Liver cancer cells |
| Sample name |
Huh-7 - 100K pellet |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 131 |
| Experiment ID |
1309 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
TSG101|CD63|CD81|Alix
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
32082513
|
| Organism |
Homo sapiens |
| Experiment description |
Hepatitis A virus structural protein pX interacts with ALIX and promotes the secretion of virions and foreign proteins through exosome-like vesicles |
| Authors |
"Jiang W, Ma P, Deng L, Liu Z, Wang X, Liu X, Long G." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2020 |
| Sample |
Liver cancer cells |
| Sample name |
Huh-7 - 100K pellet |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 132 |
| Experiment ID |
1320 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD81|TSG101|Alix
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
32082513
|
| Organism |
Homo sapiens |
| Experiment description |
Hepatitis A virus structural protein pX interacts with ALIX and promotes the secretion of virions and foreign proteins through exosome-like vesicles |
| Authors |
"Jiang W, Ma P, Deng L, Liu Z, Wang X, Liu X, Long G." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2020 |
| Sample |
Liver cancer cells |
| Sample name |
Huh-7 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 133 |
| Experiment ID |
1371 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD81|CD63|CD9|TSG101|Alix
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36631249
|
| Organism |
Homo sapiens |
| Experiment description |
S100A10 promotes HCC development and progression via transfer in extracellular vesicles and regulating their protein cargos |
| Authors |
"Wang X, Huang H, Sze KM, Wang J, Tian L, Lu J, Tsui YM, Ma HT, Lee E, Chen A, Lee J, Wang Y, Yam JWP, Cheung TT, Guan X, Ng IO." |
| Journal name |
Gut
|
| Publication year |
2023 |
| Sample |
Liver cancer cells |
| Sample name |
PLC |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 134 |
| Experiment ID |
1372 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD81|CD63|CD9|TSG101|Alix
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36631249
|
| Organism |
Homo sapiens |
| Experiment description |
S100A10 promotes HCC development and progression via transfer in extracellular vesicles and regulating their protein cargos |
| Authors |
"Wang X, Huang H, Sze KM, Wang J, Tian L, Lu J, Tsui YM, Ma HT, Lee E, Chen A, Lee J, Wang Y, Yam JWP, Cheung TT, Guan X, Ng IO." |
| Journal name |
Gut
|
| Publication year |
2023 |
| Sample |
Liver cancer cells |
| Sample name |
PLC |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 135 |
| Experiment ID |
1373 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD81|CD63|CD9|TSG101|Alix
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36631249
|
| Organism |
Homo sapiens |
| Experiment description |
S100A10 promotes HCC development and progression via transfer in extracellular vesicles and regulating their protein cargos |
| Authors |
"Wang X, Huang H, Sze KM, Wang J, Tian L, Lu J, Tsui YM, Ma HT, Lee E, Chen A, Lee J, Wang Y, Yam JWP, Cheung TT, Guan X, Ng IO." |
| Journal name |
Gut
|
| Publication year |
2023 |
| Sample |
Liver cancer cells |
| Sample name |
97L |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 136 |
| Experiment ID |
1374 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD81|CD63|CD9|TSG101|Alix
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36631249
|
| Organism |
Homo sapiens |
| Experiment description |
S100A10 promotes HCC development and progression via transfer in extracellular vesicles and regulating their protein cargos |
| Authors |
"Wang X, Huang H, Sze KM, Wang J, Tian L, Lu J, Tsui YM, Ma HT, Lee E, Chen A, Lee J, Wang Y, Yam JWP, Cheung TT, Guan X, Ng IO." |
| Journal name |
Gut
|
| Publication year |
2023 |
| Sample |
Liver cancer cells |
| Sample name |
97L |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 137 |
| Experiment ID |
1375 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD81|CD63|CD9|TSG101|Alix
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36631249
|
| Organism |
Homo sapiens |
| Experiment description |
S100A10 promotes HCC development and progression via transfer in extracellular vesicles and regulating their protein cargos |
| Authors |
"Wang X, Huang H, Sze KM, Wang J, Tian L, Lu J, Tsui YM, Ma HT, Lee E, Chen A, Lee J, Wang Y, Yam JWP, Cheung TT, Guan X, Ng IO." |
| Journal name |
Gut
|
| Publication year |
2023 |
| Sample |
Liver cancer cells |
| Sample name |
MIHA |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 138 |
| Experiment ID |
1376 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD81|CD63|CD9|TSG101|Alix
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36631249
|
| Organism |
Homo sapiens |
| Experiment description |
S100A10 promotes HCC development and progression via transfer in extracellular vesicles and regulating their protein cargos |
| Authors |
"Wang X, Huang H, Sze KM, Wang J, Tian L, Lu J, Tsui YM, Ma HT, Lee E, Chen A, Lee J, Wang Y, Yam JWP, Cheung TT, Guan X, Ng IO." |
| Journal name |
Gut
|
| Publication year |
2023 |
| Sample |
Liver cancer cells |
| Sample name |
MIHA |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 139 |
| Experiment ID |
1377 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD81|CD63|CD9|TSG101|Alix
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36631249
|
| Organism |
Homo sapiens |
| Experiment description |
S100A10 promotes HCC development and progression via transfer in extracellular vesicles and regulating their protein cargos |
| Authors |
"Wang X, Huang H, Sze KM, Wang J, Tian L, Lu J, Tsui YM, Ma HT, Lee E, Chen A, Lee J, Wang Y, Yam JWP, Cheung TT, Guan X, Ng IO." |
| Journal name |
Gut
|
| Publication year |
2023 |
| Sample |
Liver cancer cells |
| Sample name |
97H |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 140 |
| Experiment ID |
1378 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD81|CD63|CD9|TSG101|Alix
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36631249
|
| Organism |
Homo sapiens |
| Experiment description |
S100A10 promotes HCC development and progression via transfer in extracellular vesicles and regulating their protein cargos |
| Authors |
"Wang X, Huang H, Sze KM, Wang J, Tian L, Lu J, Tsui YM, Ma HT, Lee E, Chen A, Lee J, Wang Y, Yam JWP, Cheung TT, Guan X, Ng IO." |
| Journal name |
Gut
|
| Publication year |
2023 |
| Sample |
Liver cancer cells |
| Sample name |
97H |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 141 |
| Experiment ID |
1381 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD63|CD81|TSG101|Alix
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36631249
|
| Organism |
Homo sapiens |
| Experiment description |
S100A10 promotes HCC development and progression via transfer in extracellular vesicles and regulating their protein cargos |
| Authors |
"Wang X, Huang H, Sze KM, Wang J, Tian L, Lu J, Tsui YM, Ma HT, Lee E, Chen A, Lee J, Wang Y, Yam JWP, Cheung TT, Guan X, Ng IO." |
| Journal name |
Gut
|
| Publication year |
2023 |
| Sample |
Liver cancer cells |
| Sample name |
97H |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 142 |
| Experiment ID |
1382 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD63|CD81|TSG101|Alix
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36631249
|
| Organism |
Homo sapiens |
| Experiment description |
S100A10 promotes HCC development and progression via transfer in extracellular vesicles and regulating their protein cargos |
| Authors |
"Wang X, Huang H, Sze KM, Wang J, Tian L, Lu J, Tsui YM, Ma HT, Lee E, Chen A, Lee J, Wang Y, Yam JWP, Cheung TT, Guan X, Ng IO." |
| Journal name |
Gut
|
| Publication year |
2023 |
| Sample |
Liver cancer cells |
| Sample name |
97H |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 143 |
| Experiment ID |
83 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
TSG101|Alix
|
Enriched markers |
|
✔
HSPA5
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
20949044
|
| Organism |
Homo sapiens |
| Experiment description |
Let-7 MicroRNA Family Is Selectively Secreted into the Extracellular Environment via Exosomes in a Metastatic Gastric Cancer Cell Line |
| Authors |
"Ohshima K, Inoue K, Fujiwara A, Hatakeyama K, Kanto K, Watanabe Y, Muramatsu K, Fukuda Y, Ogura S, Yamaguchi K, Mochizuki T." |
| Journal name |
PLoS One
|
| Publication year |
2010 |
| Sample |
Lung cancer cells |
| Sample name |
SBC-3 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
miRNA |
| Methods used in the study |
Western blotting
RT-PCR
Human miRNA Microarray Kit |
|
|
| 144 |
| Experiment ID |
731 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
TSG101|SDCBP
|
Enriched markers |
|
✔
GOLGA2|TUBA1A
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35136055
|
| Organism |
Homo sapiens |
| Experiment description |
"Syntenin-1-mediated small extracellular vesicles promotes cell growth, migration, and angiogenesis by increasing onco-miRNAs secretion in lung cancer cells" |
| Authors |
"Kim O, Hwangbo C, Tran PT, Lee JH." |
| Journal name |
Cell Death Dis
|
| Publication year |
2022 |
| Sample |
Lung cancer cells |
| Sample name |
NCI-H226 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
miRNA |
| Methods used in the study |
Western blotting
miRNA array |
|
|
| 145 |
| Experiment ID |
732 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
TSG101|SDCBP
|
Enriched markers |
|
✔
GOLGA2|TUBA1A
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35136055
|
| Organism |
Homo sapiens |
| Experiment description |
"Syntenin-1-mediated small extracellular vesicles promotes cell growth, migration, and angiogenesis by increasing onco-miRNAs secretion in lung cancer cells" |
| Authors |
"Kim O, Hwangbo C, Tran PT, Lee JH." |
| Journal name |
Cell Death Dis
|
| Publication year |
2022 |
| Sample |
Lung cancer cells |
| Sample name |
NCI-H226 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
miRNA |
| Methods used in the study |
Western blotting
miRNA array |
|
|
| 146 |
| Experiment ID |
733 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
TSG101|SDCBP
|
Enriched markers |
|
✔
TUBA1A
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35136055
|
| Organism |
Homo sapiens |
| Experiment description |
"Syntenin-1-mediated small extracellular vesicles promotes cell growth, migration, and angiogenesis by increasing onco-miRNAs secretion in lung cancer cells" |
| Authors |
"Kim O, Hwangbo C, Tran PT, Lee JH." |
| Journal name |
Cell Death Dis
|
| Publication year |
2022 |
| Sample |
Lung cancer cells |
| Sample name |
BZR |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 147 |
| Experiment ID |
736 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
TSG101|SDCBP
|
Enriched markers |
|
✔
TUBA1A
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35136055
|
| Organism |
Homo sapiens |
| Experiment description |
"Syntenin-1-mediated small extracellular vesicles promotes cell growth, migration, and angiogenesis by increasing onco-miRNAs secretion in lung cancer cells" |
| Authors |
"Kim O, Hwangbo C, Tran PT, Lee JH." |
| Journal name |
Cell Death Dis
|
| Publication year |
2022 |
| Sample |
Lung cancer cells |
| Sample name |
A549 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
miRNA |
| Methods used in the study |
Western blotting
RT-qPCR |
|
|
| 148 |
| Experiment ID |
737 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
TSG101|SDCBP
|
Enriched markers |
|
✔
TUBA1A
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35136055
|
| Organism |
Homo sapiens |
| Experiment description |
"Syntenin-1-mediated small extracellular vesicles promotes cell growth, migration, and angiogenesis by increasing onco-miRNAs secretion in lung cancer cells" |
| Authors |
"Kim O, Hwangbo C, Tran PT, Lee JH." |
| Journal name |
Cell Death Dis
|
| Publication year |
2022 |
| Sample |
Lung cancer cells |
| Sample name |
NCI-H1703 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
miRNA |
| Methods used in the study |
Western blotting
RT-qPCR |
|
|
| 149 |
| Experiment ID |
738 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
TSG101|SDCBP
|
Enriched markers |
|
✔
TUBA1A
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35136055
|
| Organism |
Homo sapiens |
| Experiment description |
"Syntenin-1-mediated small extracellular vesicles promotes cell growth, migration, and angiogenesis by increasing onco-miRNAs secretion in lung cancer cells" |
| Authors |
"Kim O, Hwangbo C, Tran PT, Lee JH." |
| Journal name |
Cell Death Dis
|
| Publication year |
2022 |
| Sample |
Lung cancer cells |
| Sample name |
NCI-H1703 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
miRNA |
| Methods used in the study |
Western blotting
RT-qPCR |
|
|
| 150 |
| Experiment ID |
743 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
TSG101|SDCBP
|
Enriched markers |
|
✔
TUBA1A
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35136055
|
| Organism |
Homo sapiens |
| Experiment description |
"Syntenin-1-mediated small extracellular vesicles promotes cell growth, migration, and angiogenesis by increasing onco-miRNAs secretion in lung cancer cells" |
| Authors |
"Kim O, Hwangbo C, Tran PT, Lee JH." |
| Journal name |
Cell Death Dis
|
| Publication year |
2022 |
| Sample |
Lung cancer cells |
| Sample name |
Calu-3 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 151 |
| Experiment ID |
744 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
TSG101|SDCBP
|
Enriched markers |
|
✔
TUBA1A
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35136055
|
| Organism |
Homo sapiens |
| Experiment description |
"Syntenin-1-mediated small extracellular vesicles promotes cell growth, migration, and angiogenesis by increasing onco-miRNAs secretion in lung cancer cells" |
| Authors |
"Kim O, Hwangbo C, Tran PT, Lee JH." |
| Journal name |
Cell Death Dis
|
| Publication year |
2022 |
| Sample |
Lung cancer cells |
| Sample name |
Calu-3 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 152 |
| Experiment ID |
986 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|TSG101|GAPDH
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
30089911
|
| Organism |
Homo sapiens |
| Experiment description |
Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response |
| Authors |
"Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W." |
| Journal name |
Nature
|
| Publication year |
2018 |
| Sample |
Lung cancer cells |
| Sample name |
H1299 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 153 |
| Experiment ID |
987 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|TSG101|GAPDH
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
30089911
|
| Organism |
Homo sapiens |
| Experiment description |
Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response |
| Authors |
"Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W." |
| Journal name |
Nature
|
| Publication year |
2018 |
| Sample |
Lung cancer cells |
| Sample name |
H358 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 154 |
| Experiment ID |
988 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|TSG101|GAPDH
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
30089911
|
| Organism |
Homo sapiens |
| Experiment description |
Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response |
| Authors |
"Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W." |
| Journal name |
Nature
|
| Publication year |
2018 |
| Sample |
Lung cancer cells |
| Sample name |
H1264 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 155 |
| Experiment ID |
1067 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
TSG101|CD63|GAPDH
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34500436
|
| Organism |
Homo sapiens |
| Experiment description |
"MiR-185-5p targets RAB35 gene to regulate tumor cell-derived exosomes-mediated proliferation, migration and invasion of non-small cell lung cancer cells" |
| Authors |
"Wen H, Liu Z, Tang J, Bu L." |
| Journal name |
Aging
|
| Publication year |
2021 |
| Sample |
Lung cancer cells |
| Sample name |
A549 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 156 |
| Experiment ID |
1068 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
TSG101|CD63|GAPDH
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34500436
|
| Organism |
Homo sapiens |
| Experiment description |
"MiR-185-5p targets RAB35 gene to regulate tumor cell-derived exosomes-mediated proliferation, migration and invasion of non-small cell lung cancer cells" |
| Authors |
"Wen H, Liu Z, Tang J, Bu L." |
| Journal name |
Aging
|
| Publication year |
2021 |
| Sample |
Lung cancer cells |
| Sample name |
A549 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 157 |
| Experiment ID |
1069 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
TSG101|CD63|GAPDH
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34500436
|
| Organism |
Homo sapiens |
| Experiment description |
"MiR-185-5p targets RAB35 gene to regulate tumor cell-derived exosomes-mediated proliferation, migration and invasion of non-small cell lung cancer cells" |
| Authors |
"Wen H, Liu Z, Tang J, Bu L." |
| Journal name |
Aging
|
| Publication year |
2021 |
| Sample |
Lung cancer cells |
| Sample name |
A549 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 158 |
| Experiment ID |
1070 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
TSG101|CD63|GAPDH
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34500436
|
| Organism |
Homo sapiens |
| Experiment description |
"MiR-185-5p targets RAB35 gene to regulate tumor cell-derived exosomes-mediated proliferation, migration and invasion of non-small cell lung cancer cells" |
| Authors |
"Wen H, Liu Z, Tang J, Bu L." |
| Journal name |
Aging
|
| Publication year |
2021 |
| Sample |
Lung cancer cells |
| Sample name |
A549 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 159 |
| Experiment ID |
1071 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
TSG101|CD63|GAPDH
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34500436
|
| Organism |
Homo sapiens |
| Experiment description |
"MiR-185-5p targets RAB35 gene to regulate tumor cell-derived exosomes-mediated proliferation, migration and invasion of non-small cell lung cancer cells" |
| Authors |
"Wen H, Liu Z, Tang J, Bu L." |
| Journal name |
Aging
|
| Publication year |
2021 |
| Sample |
Lung cancer cells |
| Sample name |
A549 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 160 |
| Experiment ID |
1072 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
TSG101|CD63|GAPDH
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34500436
|
| Organism |
Homo sapiens |
| Experiment description |
"MiR-185-5p targets RAB35 gene to regulate tumor cell-derived exosomes-mediated proliferation, migration and invasion of non-small cell lung cancer cells" |
| Authors |
"Wen H, Liu Z, Tang J, Bu L." |
| Journal name |
Aging
|
| Publication year |
2021 |
| Sample |
Lung cancer cells |
| Sample name |
H2170 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 161 |
| Experiment ID |
1073 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
TSG101|CD63|GAPDH
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34500436
|
| Organism |
Homo sapiens |
| Experiment description |
"MiR-185-5p targets RAB35 gene to regulate tumor cell-derived exosomes-mediated proliferation, migration and invasion of non-small cell lung cancer cells" |
| Authors |
"Wen H, Liu Z, Tang J, Bu L." |
| Journal name |
Aging
|
| Publication year |
2021 |
| Sample |
Lung cancer cells |
| Sample name |
H2170 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 162 |
| Experiment ID |
1074 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
TSG101|CD63|GAPDH
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34500436
|
| Organism |
Homo sapiens |
| Experiment description |
"MiR-185-5p targets RAB35 gene to regulate tumor cell-derived exosomes-mediated proliferation, migration and invasion of non-small cell lung cancer cells" |
| Authors |
"Wen H, Liu Z, Tang J, Bu L." |
| Journal name |
Aging
|
| Publication year |
2021 |
| Sample |
Lung cancer cells |
| Sample name |
H2170 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 163 |
| Experiment ID |
1075 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
TSG101|CD63|GAPDH
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34500436
|
| Organism |
Homo sapiens |
| Experiment description |
"MiR-185-5p targets RAB35 gene to regulate tumor cell-derived exosomes-mediated proliferation, migration and invasion of non-small cell lung cancer cells" |
| Authors |
"Wen H, Liu Z, Tang J, Bu L." |
| Journal name |
Aging
|
| Publication year |
2021 |
| Sample |
Lung cancer cells |
| Sample name |
H2170 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 164 |
| Experiment ID |
1076 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
TSG101|CD63|GAPDH
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34500436
|
| Organism |
Homo sapiens |
| Experiment description |
"MiR-185-5p targets RAB35 gene to regulate tumor cell-derived exosomes-mediated proliferation, migration and invasion of non-small cell lung cancer cells" |
| Authors |
"Wen H, Liu Z, Tang J, Bu L." |
| Journal name |
Aging
|
| Publication year |
2021 |
| Sample |
Lung cancer cells |
| Sample name |
H2170 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 165 |
| Experiment ID |
1084 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
TSG101|CD63
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34497265
|
| Organism |
Homo sapiens |
| Experiment description |
"Tumor-derived exosomal miR-3157-3p promotes angiogenesis, vascular permeability and metastasis by targeting TIMP/KLF2 in non-small cell lung cancer" |
| Authors |
"Ma Z, Wei K, Yang F, Guo Z, Pan C, He Y, Wang J, Li Z, Chen L, Chen Y, Xia Y." |
| Journal name |
Cell Death Dis
|
| Publication year |
2021 |
| Sample |
Lung cancer cells |
| Sample name |
A549 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 166 |
| Experiment ID |
1085 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
TSG101|CD63
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34497265
|
| Organism |
Homo sapiens |
| Experiment description |
"Tumor-derived exosomal miR-3157-3p promotes angiogenesis, vascular permeability and metastasis by targeting TIMP/KLF2 in non-small cell lung cancer" |
| Authors |
"Ma Z, Wei K, Yang F, Guo Z, Pan C, He Y, Wang J, Li Z, Chen L, Chen Y, Xia Y." |
| Journal name |
Cell Death Dis
|
| Publication year |
2021 |
| Sample |
Lung cancer cells |
| Sample name |
H1299 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 167 |
| Experiment ID |
1324 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
GAPDH|CD63|LAMP1|FLOT1|TSG101|Alix
|
Enriched markers |
|
✔
GAPDH|HSP90AA1
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
33686255
|
| Organism |
Homo sapiens |
| Experiment description |
Extracellular vesicle drug occupancy enables real-time monitoring of targeted cancer therapy. |
| Authors |
"Pan S, Zhang Y, Natalia A, Lim CZJ, Ho NRY, Chowbay B, Loh TP, Tam JKC, Shao H." |
| Journal name |
Nat Nanotechnol
|
| Publication year |
2021 |
| Sample |
Lung cancer cells |
| Sample name |
H3255 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 168 |
| Experiment ID |
417 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
|
Enriched markers |
|
✔
CANX|ACTB
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Lymphoma cells |
| Sample name |
Raji |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
mRNA |
| Methods used in the study |
RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry |
|
|
| 169 |
| Experiment ID |
411 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Mammary cancer-associated fibroblasts |
| Sample name |
mCAF |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
mRNA |
| Methods used in the study |
RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry |
|
|
| 170 |
| Experiment ID |
793 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|CD9|TSG101
|
Enriched markers |
|
✔
HSP90AA1
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35649370
|
| Organism |
Homo sapiens |
| Experiment description |
Rab39 and its effector UACA regulate basolateral exosome release from polarized epithelial cells |
| Authors |
"Matsui T, Sakamaki Y, Nakashima S, Fukuda M." |
| Journal name |
Cell Rep
|
| Publication year |
2022 |
| Sample |
Mammary epithelial cells |
| Sample name |
MCF10A |
| Isolation/purification methods |
Differential centrifugation
Filtration
Immunoaffinity capture
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 171 |
| Experiment ID |
794 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|CD9|TSG101
|
Enriched markers |
|
✔
HSP90AA1
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35649370
|
| Organism |
Homo sapiens |
| Experiment description |
Rab39 and its effector UACA regulate basolateral exosome release from polarized epithelial cells |
| Authors |
"Matsui T, Sakamaki Y, Nakashima S, Fukuda M." |
| Journal name |
Cell Rep
|
| Publication year |
2022 |
| Sample |
Mammary epithelial cells |
| Sample name |
MCF10A |
| Isolation/purification methods |
Differential centrifugation
Filtration
Immunoaffinity capture
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 172 |
| Experiment ID |
795 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|CD9|TSG101
|
Enriched markers |
|
✔
HSP90AA1
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35649370
|
| Organism |
Homo sapiens |
| Experiment description |
Rab39 and its effector UACA regulate basolateral exosome release from polarized epithelial cells |
| Authors |
"Matsui T, Sakamaki Y, Nakashima S, Fukuda M." |
| Journal name |
Cell Rep
|
| Publication year |
2022 |
| Sample |
Mammary epithelial cells |
| Sample name |
MCF10A |
| Isolation/purification methods |
Differential centrifugation
Filtration
Immunoaffinity capture
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 173 |
| Experiment ID |
796 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|CD9|TSG101
|
Enriched markers |
|
✔
HSP90AA1
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35649370
|
| Organism |
Homo sapiens |
| Experiment description |
Rab39 and its effector UACA regulate basolateral exosome release from polarized epithelial cells |
| Authors |
"Matsui T, Sakamaki Y, Nakashima S, Fukuda M." |
| Journal name |
Cell Rep
|
| Publication year |
2022 |
| Sample |
Mammary epithelial cells |
| Sample name |
MCF10A |
| Isolation/purification methods |
Differential centrifugation
Filtration
Immunoaffinity capture
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 174 |
| Experiment ID |
254 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
TSG101|FLOT1|CD81
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
Western blotting
|
| PubMed ID |
25950383
|
| Organism |
Homo sapiens |
| Experiment description |
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines |
| Authors |
"Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L" |
| Journal name |
Pigment Cell Melanoma Res
|
| Publication year |
2015 |
| Sample |
Melanoma cells |
| Sample name |
MNT-1 |
| Isolation/purification methods |
Differential centrifugation
Unltracentrifugation
Sucrose density gradient |
| Flotation density |
1.13 - 1.19 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 175 |
| Experiment ID |
255 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
TSG101|FLOT1
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
Western blotting
|
| PubMed ID |
25950383
|
| Organism |
Homo sapiens |
| Experiment description |
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines |
| Authors |
"Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L" |
| Journal name |
Pigment Cell Melanoma Res
|
| Publication year |
2015 |
| Sample |
Melanoma cells |
| Sample name |
G1 |
| Isolation/purification methods |
Differential centrifugation
Unltracentrifugation
Sucrose density gradient |
| Flotation density |
1.13 - 1.19 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 176 |
| Experiment ID |
256 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
TSG101|FLOT1
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
Western blotting
|
| PubMed ID |
25950383
|
| Organism |
Homo sapiens |
| Experiment description |
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines |
| Authors |
"Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L" |
| Journal name |
Pigment Cell Melanoma Res
|
| Publication year |
2015 |
| Sample |
Melanoma cells |
| Sample name |
501mel |
| Isolation/purification methods |
Differential centrifugation
Unltracentrifugation
Sucrose density gradient |
| Flotation density |
1.13 - 1.19 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 177 |
| Experiment ID |
257 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
TSG101|FLOT1
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
Western blotting
|
| PubMed ID |
25950383
|
| Organism |
Homo sapiens |
| Experiment description |
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines |
| Authors |
"Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L" |
| Journal name |
Pigment Cell Melanoma Res
|
| Publication year |
2015 |
| Sample |
Melanoma cells |
| Sample name |
Daju |
| Isolation/purification methods |
Differential centrifugation
Unltracentrifugation
Sucrose density gradient |
| Flotation density |
1.13 - 1.19 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 178 |
| Experiment ID |
258 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
TSG101|FLOT1|CD81
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
Western blotting
|
| PubMed ID |
25950383
|
| Organism |
Homo sapiens |
| Experiment description |
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines |
| Authors |
"Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L" |
| Journal name |
Pigment Cell Melanoma Res
|
| Publication year |
2015 |
| Sample |
Melanoma cells |
| Sample name |
SKMEL28 |
| Isolation/purification methods |
Differential centrifugation
Unltracentrifugation
Sucrose density gradient |
| Flotation density |
1.13 - 1.19 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 179 |
| Experiment ID |
259 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
TSG101|FLOT1
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
Western blotting
|
| PubMed ID |
25950383
|
| Organism |
Homo sapiens |
| Experiment description |
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines |
| Authors |
"Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L" |
| Journal name |
Pigment Cell Melanoma Res
|
| Publication year |
2015 |
| Sample |
Melanoma cells |
| Sample name |
A375M |
| Isolation/purification methods |
Differential centrifugation
Unltracentrifugation
Sucrose density gradient |
| Flotation density |
1.13 - 1.19 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 180 |
| Experiment ID |
260 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
TSG101|FLOT1|CD81
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
Western blotting
|
| PubMed ID |
25950383
|
| Organism |
Homo sapiens |
| Experiment description |
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines |
| Authors |
"Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L" |
| Journal name |
Pigment Cell Melanoma Res
|
| Publication year |
2015 |
| Sample |
Melanoma cells |
| Sample name |
1205Lu |
| Isolation/purification methods |
Differential centrifugation
Unltracentrifugation
Sucrose density gradient |
| Flotation density |
1.13 - 1.19 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 181 |
| Experiment ID |
265 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
TSG101|FLOT1
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
26176991
|
| Organism |
Homo sapiens |
| Experiment description |
Small RNA deep sequencing discriminates subsets of extracellular vesicles released by melanoma cells - evidence of unique microRNA cargos |
| Authors |
"Lunavat TR, Cheng L, Kim DK, Bhadury J, Jang SC, Lasser C, Sharples RA, Lopez MD, Nilsson J, Gho YS, Hill AF, Lotvall J." |
| Journal name |
RNA Biology
|
| Publication year |
2015 |
| Sample |
Melanoma cells |
| Sample name |
MML-1 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
miRNA
snRNA
snoRNA
mtRNA
Ro associated Y-RNA
MiscRNA |
| Methods used in the study |
Western blotting
RNA deep sequencing |
|
|
| 182 |
| Experiment ID |
955 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|TSG101|GAPDH
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
30089911
|
| Organism |
Homo sapiens |
| Experiment description |
Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response |
| Authors |
"Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W." |
| Journal name |
Nature
|
| Publication year |
2018 |
| Sample |
Melanoma cells |
| Sample name |
A375 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Reverse phase protein array |
|
|
| 183 |
| Experiment ID |
956 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|TSG101|GAPDH
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
30089911
|
| Organism |
Homo sapiens |
| Experiment description |
Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response |
| Authors |
"Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W." |
| Journal name |
Nature
|
| Publication year |
2018 |
| Sample |
Melanoma cells |
| Sample name |
UACC-903 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Reverse phase protein array |
|
|
| 184 |
| Experiment ID |
957 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD63|TSG101|GAPDH
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
30089911
|
| Organism |
Homo sapiens |
| Experiment description |
Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response |
| Authors |
"Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W." |
| Journal name |
Nature
|
| Publication year |
2018 |
| Sample |
Melanoma cells |
| Sample name |
WM9 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Reverse phase protein array
Immuno-EM
ELISA |
|
|
| 185 |
| Experiment ID |
958 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|TSG101|GAPDH
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
30089911
|
| Organism |
Homo sapiens |
| Experiment description |
Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response |
| Authors |
"Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W." |
| Journal name |
Nature
|
| Publication year |
2018 |
| Sample |
Melanoma cells |
| Sample name |
WM164 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Reverse phase protein array
ELISA |
|
|
| 186 |
| Experiment ID |
963 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|TSG101
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
30089911
|
| Organism |
Homo sapiens |
| Experiment description |
Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response |
| Authors |
"Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W." |
| Journal name |
Nature
|
| Publication year |
2018 |
| Sample |
Melanoma cells |
| Sample name |
WM9 - Fraction 6 |
| Isolation/purification methods |
Differential centrifugation
Iodixanol density gradient centrifugation
Ultracentrifugation |
| Flotation density |
1.131 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 187 |
| Experiment ID |
964 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|TSG101
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
30089911
|
| Organism |
Homo sapiens |
| Experiment description |
Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response |
| Authors |
"Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W." |
| Journal name |
Nature
|
| Publication year |
2018 |
| Sample |
Melanoma cells |
| Sample name |
WM9 - Fraction 7 |
| Isolation/purification methods |
Differential centrifugation
Iodixanol density gradient centrifugation
Ultracentrifugation |
| Flotation density |
1.144 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 188 |
| Experiment ID |
965 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|TSG101
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
30089911
|
| Organism |
Homo sapiens |
| Experiment description |
Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response |
| Authors |
"Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W." |
| Journal name |
Nature
|
| Publication year |
2018 |
| Sample |
Melanoma cells |
| Sample name |
WM9 - Fraction 8 |
| Isolation/purification methods |
Differential centrifugation
Iodixanol density gradient centrifugation
Ultracentrifugation |
| Flotation density |
1.156 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 189 |
| Experiment ID |
966 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|TSG101
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
30089911
|
| Organism |
Homo sapiens |
| Experiment description |
Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response |
| Authors |
"Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W." |
| Journal name |
Nature
|
| Publication year |
2018 |
| Sample |
Melanoma cells |
| Sample name |
WM9 - Fraction 9 |
| Isolation/purification methods |
Differential centrifugation
Iodixanol density gradient centrifugation
Ultracentrifugation |
| Flotation density |
1.178 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 190 |
| Experiment ID |
967 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|TSG101
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
30089911
|
| Organism |
Homo sapiens |
| Experiment description |
Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response |
| Authors |
"Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W." |
| Journal name |
Nature
|
| Publication year |
2018 |
| Sample |
Melanoma cells |
| Sample name |
WM9 - Fraction 10 |
| Isolation/purification methods |
Differential centrifugation
Iodixanol density gradient centrifugation
Ultracentrifugation |
| Flotation density |
1.193 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 191 |
| Experiment ID |
971 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|TSG101|GAPDH
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
30089911
|
| Organism |
Homo sapiens |
| Experiment description |
Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response |
| Authors |
"Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W." |
| Journal name |
Nature
|
| Publication year |
2018 |
| Sample |
Melanoma cells |
| Sample name |
WM9 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 192 |
| Experiment ID |
972 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|TSG101|GAPDH
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
30089911
|
| Organism |
Homo sapiens |
| Experiment description |
Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response |
| Authors |
"Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W." |
| Journal name |
Nature
|
| Publication year |
2018 |
| Sample |
Melanoma cells |
| Sample name |
WM9 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 193 |
| Experiment ID |
973 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|TSG101|GAPDH
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
30089911
|
| Organism |
Homo sapiens |
| Experiment description |
Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response |
| Authors |
"Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W." |
| Journal name |
Nature
|
| Publication year |
2018 |
| Sample |
Melanoma cells |
| Sample name |
WM9 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 194 |
| Experiment ID |
974 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|TSG101|GAPDH
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
30089911
|
| Organism |
Homo sapiens |
| Experiment description |
Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response |
| Authors |
"Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W." |
| Journal name |
Nature
|
| Publication year |
2018 |
| Sample |
Melanoma cells |
| Sample name |
WM9 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 195 |
| Experiment ID |
975 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|TSG101|GAPDH
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
30089911
|
| Organism |
Homo sapiens |
| Experiment description |
Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response |
| Authors |
"Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W." |
| Journal name |
Nature
|
| Publication year |
2018 |
| Sample |
Melanoma cells |
| Sample name |
WM9 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 196 |
| Experiment ID |
976 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|TSG101|GAPDH
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
30089911
|
| Organism |
Homo sapiens |
| Experiment description |
Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response |
| Authors |
"Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W." |
| Journal name |
Nature
|
| Publication year |
2018 |
| Sample |
Melanoma cells |
| Sample name |
WM9 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 197 |
| Experiment ID |
984 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|TSG101|GAPDH
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
30089911
|
| Organism |
Homo sapiens |
| Experiment description |
Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response |
| Authors |
"Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W." |
| Journal name |
Nature
|
| Publication year |
2018 |
| Sample |
Melanoma cells |
| Sample name |
MEL624 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 198 |
| Experiment ID |
985 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|TSG101|GAPDH
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
30089911
|
| Organism |
Homo sapiens |
| Experiment description |
Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response |
| Authors |
"Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W." |
| Journal name |
Nature
|
| Publication year |
2018 |
| Sample |
Melanoma cells |
| Sample name |
MEL624 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Reverse phase protein array
Western blotting
ELISA |
|
|
| 199 |
| Experiment ID |
372 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|TSG101
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
33479185
|
| Organism |
Homo sapiens |
| Experiment description |
Human umbilical cord mesenchymal stem cell-derived exosomal miR-146a-5p reduces microglial-mediated neuroinflammation via suppression of the IRAK1/TRAF6 signaling pathway after ischemic stroke |
| Authors |
"Zhang Z, Zou X, Zhang R, Xie Y, Feng Z, Li F, Han J, Sun H, Ouyang Q, Hua S, Lv B, Hua T, Liu Z, Cai Y, Zou Y, Tang Y, Jiang X." |
| Journal name |
Aging
|
| Publication year |
2021 |
| Sample |
Mesenchymal stem cells |
| Sample name |
hUMSCs |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
miRNA |
| Methods used in the study |
Western blotting
Microarray |
|
|
| 200 |
| Experiment ID |
488 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
36408942
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells |
| Authors |
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ." |
| Journal name |
Proteomics
|
| Publication year |
2023 |
| Sample |
Mesenchymal stem cells |
| Sample name |
UCMSC |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectometry |
|
|
| 201 |
| Experiment ID |
418 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Monocytic leukemia cells |
| Sample name |
THP-1 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
mRNA |
| Methods used in the study |
RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry |
|
|
| 202 |
| Experiment ID |
719 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD63|CD9|TSG101
|
Enriched markers |
|
✔
ACTB
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34388111
|
| Organism |
Homo sapiens |
| Experiment description |
miRNA-221-3p derived from M2-polarized tumor-associated macrophage exosomes aggravates the growth and metastasis of osteosarcoma through SOCS3/JAK2/STAT3 axis |
| Authors |
"Liu W, Long Q, Zhang W, Zeng D, Hu B, Liu S, Chen L." |
| Journal name |
Aging
|
| Publication year |
2021 |
| Sample |
Monocytic leukemia cells |
| Sample name |
THP1 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
miRNA |
| Methods used in the study |
Western blotting
qRT-PCR |
|
|
| 203 |
| Experiment ID |
720 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|CD9|TSG101
|
Enriched markers |
|
✔
ACTB
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34388111
|
| Organism |
Homo sapiens |
| Experiment description |
miRNA-221-3p derived from M2-polarized tumor-associated macrophage exosomes aggravates the growth and metastasis of osteosarcoma through SOCS3/JAK2/STAT3 axis |
| Authors |
"Liu W, Long Q, Zhang W, Zeng D, Hu B, Liu S, Chen L." |
| Journal name |
Aging
|
| Publication year |
2021 |
| Sample |
Monocytic leukemia cells |
| Sample name |
THP1 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
miRNA |
| Methods used in the study |
Western blotting
qRT-PCR |
|
|
| 204 |
| Experiment ID |
721 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|CD9|TSG101
|
Enriched markers |
|
✔
ACTB
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34388111
|
| Organism |
Homo sapiens |
| Experiment description |
miRNA-221-3p derived from M2-polarized tumor-associated macrophage exosomes aggravates the growth and metastasis of osteosarcoma through SOCS3/JAK2/STAT3 axis |
| Authors |
"Liu W, Long Q, Zhang W, Zeng D, Hu B, Liu S, Chen L." |
| Journal name |
Aging
|
| Publication year |
2021 |
| Sample |
Monocytic leukemia cells |
| Sample name |
THP1 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
miRNA |
| Methods used in the study |
Western blotting
qRT-PCR |
|
|
| 205 |
| Experiment ID |
722 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|CD9|TSG101
|
Enriched markers |
|
✔
ACTB
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34388111
|
| Organism |
Homo sapiens |
| Experiment description |
miRNA-221-3p derived from M2-polarized tumor-associated macrophage exosomes aggravates the growth and metastasis of osteosarcoma through SOCS3/JAK2/STAT3 axis |
| Authors |
"Liu W, Long Q, Zhang W, Zeng D, Hu B, Liu S, Chen L." |
| Journal name |
Aging
|
| Publication year |
2021 |
| Sample |
Monocytic leukemia cells |
| Sample name |
THP1 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
miRNA |
| Methods used in the study |
Western blotting
qRT-PCR |
|
|
| 206 |
| Experiment ID |
723 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|CD9|TSG101
|
Enriched markers |
|
✔
ACTB
|
| | | | |