Gene ontology annotations for TSG101
Experiment description of studies that identified TSG101 in exosomes
1
Experiment ID
116
ISEV standards
✘
EV Biophysical techniques
✘
EV Cytosolic markers
✔
CD63
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
21505438
Organism
Homo sapiens
Experiment description
Unidirectional transfer of microRNA-loaded exosomes from T cells to antigen-presenting cells.
Authors
Mittelbrunn M, Gutierrez-Vázquez C, Villarroya-Beltri C, González S, Sánchez-Cabo F, González MÁ, Bernad A, Sánchez-Madrid F.
Journal name
NATCOM
Publication year
2011
Sample
B cells
Sample name
Raji
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein miRNA
Methods used in the study
Mass spectrometry Microarray
2
Experiment ID
76
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|GAPDH|HSP90
EV Cytosolic markers
✔
CD81|CD9|CD63|LAMP1|MHCI
EV Membrane markers
✔
HSP90B1
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
20224111
Organism
Homo sapiens
Experiment description
Proteomics analysis of bladder cancer exosomes.
Authors
Welton JL, Khanna S, Giles PJ, Brennan P, Brewis IA, Staffurth J, Mason MD, Clayton A.
Journal name
MCP
Publication year
2010
Sample
Bladder cancer cells
Sample name
HT1376
Isolation/purification methods
Differential centrifugation Sucrose density gradient
Flotation density
1.10-1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [MALDI TOF/TOF] Western blotting FACS
3
Experiment ID
227
ISEV standards
✘
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✘
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
25833198
Organism
Homo sapiens
Experiment description
Guggulsterone and bexarotene induce secretion of exosome-associated breast cancer resistance protein and reduce doxorubicin resistance in MDA-MB-231 cells
Authors
Kong JN, He Q, Wang G, Dasgupta S, Dinkins MB, Zhu G, Kim A, Spassieva S, Bieberich E.
Journal name
International Journal of Cancer
Publication year
2015
Sample
Breast cancer cells
Sample name
MDA-MB-231
Isolation/purification methods
Differential ultracentrifugation Sucrose density gradient
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western Blotting
4
Experiment ID
21
ISEV standards
✔
EM|IEM
EV Biophysical techniques
✔
Alix|TSG101|HSP70
EV Cytosolic markers
✔
CD63
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry Western blotting
Supporting data
MS
WB
PubMed ID
19837982
Organism
Homo sapiens
Experiment description
Proteomic and bioinformatic analysis of immunoaffinity-purified exosomes derived from the human colon tumor cell line LIM1215.
Authors
Suresh Mathivanan, Justin W.E. Lim, Bow J. Tauro, Hong Ji, Robert L. Moritz and Richard J. Simpson
Journal name
MCP
Publication year
2009
Sample
Colorectal cancer cells
Sample name
LIM1215
Isolation/purification methods
Filtration Ultracentrifugation Sucrose density gradient
Flotation density
1.10-1.12 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [Orbitrap] Western blotting
5
Experiment ID
201
ISEV standards
✔
EM
EV Biophysical techniques
✔
Alix|TSG101|HSP70
EV Cytosolic markers
✔
CD9
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
22740476
Organism
Homo sapiens
Experiment description
Restoration of full-length APC protein in SW480 colon cancer cells induces exosome-mediated secretion of DKK-4.
Authors
Lim JW, Mathias RA, Kapp EA, Layton MJ, Faux MC, Burgess AW, Ji H, Simpson RJ.
Journal name
Electrophoresis
Publication year
2012
Sample
Colorectal cancer cells
Sample name
SW480
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
6
Experiment ID
207
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23161513
Organism
Homo sapiens
Experiment description
Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors
Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name
Mol Cell Proteomics
Publication year
2012
Sample
Colorectal cancer cells
Sample name
DKO-1
Isolation/purification methods
Differential centrifugation Filtration
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
7
Experiment ID
208
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23161513
Organism
Homo sapiens
Experiment description
Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors
Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name
Mol Cell Proteomics
Publication year
2012
Sample
Colorectal cancer cells
Sample name
Dks-8
Isolation/purification methods
Differential centrifugation Filtration
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
8
Experiment ID
209
ISEV standards
✘
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23161513
Organism
Homo sapiens
Experiment description
Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors
Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name
Mol Cell Proteomics
Publication year
2012
Sample
Colorectal cancer cells
Sample name
DLD-1
Isolation/purification methods
Differential centrifugation Filtration
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
9
Experiment ID
223
ISEV standards
✔
EM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✔
CD9
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
25330373
Organism
Homo sapiens
Experiment description
Deep sequencing of RNA from three different extracellular vesicle (EV) subtypes released from the human LIM1863 colon cancer cell line uncovers distinct miRNA-enrichment signatures
Authors
Ji H, Chen M, Greening DW, He W, Rai A, Zhang W, Simpson RJ
Journal name
PLoS One
Publication year
2014
Sample
Colorectal cancer cells
Sample name
LIM1863
Isolation/purification methods
Differential centrifugation Ultracentrifugation Immunobeads (EpCAM) Immunobeads (A33)
Flotation density
-
Molecules identified in the study
miRNA Protein
Methods used in the study
RT-PCR Western blotting
10
Experiment ID
271
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✘
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
25907253
Organism
Homo sapiens
Experiment description
Rapid and comprehensive 'shotgun' lipidome profiling of colorectal cancer cell derived exosomes.
Authors
Lydic TA, Townsend S, Adda CG, Collins C, Mathivanan S, Reid GE.
Journal name
Methods
Publication year
2015
Sample
Colorectal cancer cells
Sample name
LIM1215
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Lipids Protein
Methods used in the study
Mass spectrometry Western blotting
11
Experiment ID
282
ISEV standards
✔
CEM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✔
CD63|CD81|EpCAM
EV Membrane markers
✘
EV Negative markers
✔
DLS
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25890246
Organism
Homo sapiens
Experiment description
Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors
Xu R, Greening DW, Rai A, Ji H, Simpson RJ.
Journal name
Methods
Publication year
2015
Sample
Colorectal cancer cells
Sample name
LIM1863 - Ultracentrifugation - Rep 1
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Centrifugal concentration
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry Western blotting
12
Experiment ID
283
ISEV standards
✔
CEM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✔
CD63|CD81|EpCAM
EV Membrane markers
✘
EV Negative markers
✔
DLS
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25890246
Organism
Homo sapiens
Experiment description
Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors
Xu R, Greening DW, Rai A, Ji H, Simpson RJ.
Journal name
Methods
Publication year
2015
Sample
Colorectal cancer cells
Sample name
LIM1863 - Ultracentrifugation - Rep 2
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Centrifugal concentration
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry Western blotting
13
Experiment ID
285
ISEV standards
✔
CEM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✔
CD63|CD81|EpCAM
EV Membrane markers
✘
EV Negative markers
✔
DLS
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting Mass spectrometry
PubMed ID
25890246
Organism
Homo sapiens
Experiment description
Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors
Xu R, Greening DW, Rai A, Ji H, Simpson RJ.
Journal name
Methods
Publication year
2015
Sample
Colorectal cancer cells
Sample name
LIM1863 - Sequential centrifugal ultrafiltration - Rep 1
Isolation/purification methods
Differential centrifugation Filtration Sequential centrifugal ultrafiltration Centrifugal concentration
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry Western blotting
14
Experiment ID
286
ISEV standards
✔
CEM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✔
CD63|CD81|EpCAM
EV Membrane markers
✘
EV Negative markers
✔
DLS
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting Mass spectrometry
PubMed ID
25890246
Organism
Homo sapiens
Experiment description
Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors
Xu R, Greening DW, Rai A, Ji H, Simpson RJ.
Journal name
Methods
Publication year
2015
Sample
Colorectal cancer cells
Sample name
LIM1863 - Sequential centrifugal ultrafiltration - Rep 2
Isolation/purification methods
Differential centrifugation Filtration Sequential centrifugal ultrafiltration Centrifugal concentration
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry Western blotting
15
Experiment ID
284
ISEV standards
✘
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✘
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
25890246
Organism
Homo sapiens
Experiment description
Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors
Xu R, Greening DW, Rai A, Ji H, Simpson RJ.
Journal name
Methods
Publication year
2015
Sample
Colorectal cancer cells
Sample name
LIM1863
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient Centrifugal concentration
Flotation density
1.10-1.11 g/mL
Molecules identified in the study
Protein
Methods used in the study
Western blotting
16
Experiment ID
117
ISEV standards
✘
EV Biophysical techniques
✘
EV Cytosolic markers
✘
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
21505438
Organism
Homo sapiens
Experiment description
Unidirectional transfer of microRNA-loaded exosomes from T cells to antigen-presenting cells.
Authors
Mittelbrunn M, Gutierrez-Vázquez C, Villarroya-Beltri C, González S, Sánchez-Cabo F, González MÁ, Bernad A, Sánchez-Madrid F.
Journal name
NATCOM
Publication year
2011
Sample
Dendritic cells
Sample name
Dendritic cells
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein miRNA
Methods used in the study
Mass spectrometry Microarray
17
Experiment ID
272
ISEV standards
✔
AFM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD63
EV Membrane markers
✘
EV Negative markers
✔
DLS
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
25498500
Organism
Homo sapiens
Experiment description
Effective internalization of U251-MG-secreted exosomes into cancer cells and characterization of their lipid components.
Authors
Toda Y, Takata K, Nakagawa Y, Kawakami H, Fujioka S, Kobayashi K, Hattori Y, Kitamura Y, Akaji K, Ashihara E.
Journal name
Biochem Biophys Res Commun
Publication year
2015
Sample
Glioblastoma cells
Sample name
U251
Isolation/purification methods
Differential centrifugation Ultracenrifugation Sucrose density gradient
Flotation density
1.16 g/mL
Molecules identified in the study
Lipids Protein
Methods used in the study
Thin layer chromatography Western blotting
18
Experiment ID
234
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘
EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
26054723
Organism
Homo sapiens
Experiment description
Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors
He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name
Carcinogenesis
Publication year
2015
Sample
Hepatocellular carcinoma cells
Sample name
HKCI-C3
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient
Flotation density
1.13-1.19 g/mL
Molecules identified in the study
Protein RNA
Methods used in the study
Western blotting Mass spectrometry RT-PCR RNA Sequencing
19
Experiment ID
235
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘
EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
26054723
Organism
Homo sapiens
Experiment description
Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors
He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name
Carcinogenesis
Publication year
2015
Sample
Hepatocellular carcinoma cells
Sample name
HKCI-8
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient
Flotation density
1.13-1.19 g/mL
Molecules identified in the study
Protein RNA
Methods used in the study
Western blotting Mass spectrometry RT-PCR RNA Sequencing
20
Experiment ID
236
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘
EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
26054723
Organism
Homo sapiens
Experiment description
Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors
He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name
Carcinogenesis
Publication year
2015
Sample
Hepatocellular carcinoma cells
Sample name
MHCC97L
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient
Flotation density
1.13-1.19 g/mL
Molecules identified in the study
Protein RNA
Methods used in the study
Western blotting Mass spectrometry RT-PCR RNA Sequencing
21
Experiment ID
237
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘
EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
26054723
Organism
Homo sapiens
Experiment description
Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors
He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name
Carcinogenesis
Publication year
2015
Sample
Hepatocytes
Sample name
MIHA
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient
Flotation density
1.13-1.19 g/mL
Molecules identified in the study
Protein RNA
Methods used in the study
Western blotting Mass spectrometry RNA Sequencing
22
Experiment ID
266
ISEV standards
✘
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✘
EV Membrane markers
✔
CANX|HSP90B1
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
25265333
Organism
Homo sapiens
Experiment description
Quantitative Proteomic Analysis of Exosome Protein Content Changes Induced by Hepatitis B Virus in Huh‑7 Cells Using SILAC Labeling and LC−MS/MS
Authors
Zhao X, Wu Y, Duan J, Ma Y, Shen Z, Wei L, Cui X, Zhang J, Xie Y, Liu J
Journal name
J Proteome Res
Publication year
2014
Sample
Liver cancer cells
Sample name
Huh-7 - Wild type
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
23
Experiment ID
267
ISEV standards
✘
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✘
EV Membrane markers
✔
CANX|HSP90B1
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
25265333
Organism
Homo sapiens
Experiment description
Quantitative Proteomic Analysis of Exosome Protein Content Changes Induced by Hepatitis B Virus in Huh‑7 Cells Using SILAC Labeling and LC−MS/MS
Authors
Zhao X, Wu Y, Duan J, Ma Y, Shen Z, Wei L, Cui X, Zhang J, Xie Y, Liu J
Journal name
J Proteome Res
Publication year
2014
Sample
Liver cancer cells
Sample name
Huh-7 - Transfected with HBx plasmids
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
24
Experiment ID
268
ISEV standards
✘
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✘
EV Membrane markers
✔
CANX|HSP90B1
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
25265333
Organism
Homo sapiens
Experiment description
Quantitative Proteomic Analysis of Exosome Protein Content Changes Induced by Hepatitis B Virus in Huh‑7 Cells Using SILAC Labeling and LC−MS/MS
Authors
Zhao X, Wu Y, Duan J, Ma Y, Shen Z, Wei L, Cui X, Zhang J, Xie Y, Liu J
Journal name
J Proteome Res
Publication year
2014
Sample
Liver cancer cells
Sample name
Huh-7 - Transfected with HBx-null HBV replicon plasmids
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
25
Experiment ID
83
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✘
EV Membrane markers
✔
HSPA5
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
20949044
Organism
Homo sapiens
Experiment description
Let-7 MicroRNA Family Is Selectively Secreted into the Extracellular Environment via Exosomes in a Metastatic Gastric Cancer Cell Line
Authors
Ohshima K, Inoue K, Fujiwara A, Hatakeyama K, Kanto K, Watanabe Y, Muramatsu K, Fukuda Y, Ogura S, Yamaguchi K, Mochizuki T.
Journal name
PLoS One
Publication year
2010
Sample
Lung cancer cells
Sample name
SBC-3
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein miRNA
Methods used in the study
Western blotting RT-PCR Human miRNA Microarray Kit
26
Experiment ID
254
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
FLOT1|CD81
EV Membrane markers
✘
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry Western blotting
PubMed ID
25950383
Organism
Homo sapiens
Experiment description
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors
Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name
Pigment Cell Melanoma Res
Publication year
2015
Sample
Melanoma cells
Sample name
MNT-1
Isolation/purification methods
Differential centrifugation Unltracentrifugation Sucrose density gradient
Flotation density
1.13 - 1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
27
Experiment ID
255
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✘
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry Western blotting
PubMed ID
25950383
Organism
Homo sapiens
Experiment description
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors
Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name
Pigment Cell Melanoma Res
Publication year
2015
Sample
Melanoma cells
Sample name
G1
Isolation/purification methods
Differential centrifugation Unltracentrifugation Sucrose density gradient
Flotation density
1.13 - 1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
28
Experiment ID
256
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✘
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry Western blotting
PubMed ID
25950383
Organism
Homo sapiens
Experiment description
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors
Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name
Pigment Cell Melanoma Res
Publication year
2015
Sample
Melanoma cells
Sample name
501mel
Isolation/purification methods
Differential centrifugation Unltracentrifugation Sucrose density gradient
Flotation density
1.13 - 1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
29
Experiment ID
257
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✘
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry Western blotting
PubMed ID
25950383
Organism
Homo sapiens
Experiment description
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors
Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name
Pigment Cell Melanoma Res
Publication year
2015
Sample
Melanoma cells
Sample name
Daju
Isolation/purification methods
Differential centrifugation Unltracentrifugation Sucrose density gradient
Flotation density
1.13 - 1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
30
Experiment ID
258
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
FLOT1|CD81
EV Membrane markers
✘
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry Western blotting
PubMed ID
25950383
Organism
Homo sapiens
Experiment description
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors
Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name
Pigment Cell Melanoma Res
Publication year
2015
Sample
Melanoma cells
Sample name
SKMEL28
Isolation/purification methods
Differential centrifugation Unltracentrifugation Sucrose density gradient
Flotation density
1.13 - 1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
31
Experiment ID
259
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✘
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry Western blotting
PubMed ID
25950383
Organism
Homo sapiens
Experiment description
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors
Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name
Pigment Cell Melanoma Res
Publication year
2015
Sample
Melanoma cells
Sample name
A375M
Isolation/purification methods
Differential centrifugation Unltracentrifugation Sucrose density gradient
Flotation density
1.13 - 1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
32
Experiment ID
260
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
FLOT1|CD81
EV Membrane markers
✘
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry Western blotting
PubMed ID
25950383
Organism
Homo sapiens
Experiment description
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors
Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name
Pigment Cell Melanoma Res
Publication year
2015
Sample
Melanoma cells
Sample name
1205Lu
Isolation/purification methods
Differential centrifugation Unltracentrifugation Sucrose density gradient
Flotation density
1.13 - 1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
33
Experiment ID
265
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
CANX
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
26176991
Organism
Homo sapiens
Experiment description
Small RNA deep sequencing discriminates subsets of extracellular vesicles released by melanoma cells –evidence of unique microRNA cargos
Authors
Lunavat TR, Cheng L, Kim DK, Bhadury J, Jang SC, Lasser C, Sharples RA, Lopez MD, Nilsson J, Gho YS, Hill AF, Lotvall J.
Journal name
RNA Biology
Publication year
2015
Sample
Melanoma cells
Sample name
MML-1
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein miRNA snRNA snoRNA mtRNA Ro associated Y-RNA MiscRNA
Methods used in the study
Western blotting RNA deep sequencing
34
Experiment ID
219
ISEV standards
✔
EM
EV Biophysical techniques
✘
EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25857718
Organism
Homo sapiens
Experiment description
Proteomic analysis of exosomes from nasopharyngeal carcinoma cell identifies intercellular transfer of angiogenic proteins
Authors
Chan YK, Zhang H, Liu P, Tsao GS, Li Lung M, Mak NK, Ngok-Shun Wong R, Ying-Kit Yue P
Journal name
Int J Cancer
Publication year
2015
Sample
Nasopharyngeal carcinoma cells
Sample name
C666-1
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient
Flotation density
1.17-1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
35
Experiment ID
220
ISEV standards
✔
EM
EV Biophysical techniques
✘
EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25857718
Organism
Homo sapiens
Experiment description
Proteomic analysis of exosomes from nasopharyngeal carcinoma cell identifies intercellular transfer of angiogenic proteins
Authors
Chan YK, Zhang H, Liu P, Tsao GS, Li Lung M, Mak NK, Ngok-Shun Wong R, Ying-Kit Yue P
Journal name
Int J Cancer
Publication year
2015
Sample
Nasopharyngeal carcinoma cells
Sample name
NP69
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient
Flotation density
1.17-1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
36
Experiment ID
221
ISEV standards
✔
EM
EV Biophysical techniques
✘
EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25857718
Organism
Homo sapiens
Experiment description
Proteomic analysis of exosomes from nasopharyngeal carcinoma cell identifies intercellular transfer of angiogenic proteins
Authors
Chan YK, Zhang H, Liu P, Tsao GS, Li Lung M, Mak NK, Ngok-Shun Wong R, Ying-Kit Yue P
Journal name
Int J Cancer
Publication year
2015
Sample
Nasopharyngeal carcinoma cells
Sample name
NP460
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient
Flotation density
1.17-1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
37
Experiment ID
224
ISEV standards
✔
EM|AFM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✔
CD63|CD81
EV Membrane markers
✔
GOLGA2
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25944692
Organism
Homo sapiens
Experiment description
Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors
Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name
Oncotarget
Publication year
2015
Sample
Neuroblastoma cells
Sample name
SH-SY5Y
Isolation/purification methods
Differential centrifugation Ultracentrifugation OptiPrep density gradient
Flotation density
1.10 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry Western blotting
38
Experiment ID
239
ISEV standards
✘
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✔
CD9
EV Membrane markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
26022510
Organism
Homo sapiens
Experiment description
Serum-free culture alters the quantity and protein composition of neuroblastoma-derived extracellular vesicles
Authors
Li J, Lee Y, Johansson HJ, Mager I, Vader P, Nordin JZ, Wiklander OP, Lehtio J, Wood MJ6, Andaloussi SE.
Journal name
J Extracell Vesicles
Publication year
2015
Sample
Neuroblastoma cells
Sample name
N2a OptiMEM - Rep 1
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
1.10-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
39
Experiment ID
242
ISEV standards
✘
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✘
EV Membrane markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
26022510
Organism
Homo sapiens
Experiment description
Serum-free culture alters the quantity and protein composition of neuroblastoma-derived extracellular vesicles
Authors
Li J, Lee Y, Johansson HJ, Mager I, Vader P, Nordin JZ, Wiklander OP, Lehtio J, Wood MJ6, Andaloussi SE.
Journal name
J Extracell Vesicles
Publication year
2015
Sample
Neuroblastoma cells
Sample name
N2a Pre-spun - Rep 1
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
1.10-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
40
Experiment ID
211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23333927
Organism
Homo sapiens
Experiment description
Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors
Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name
J Proteomics
Publication year
2013
Sample
Ovarian cancer cells
Sample name
IGROV1
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
1.09-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
41
Experiment ID
212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23333927
Organism
Homo sapiens
Experiment description
Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors
Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name
J Proteomics
Publication year
2013
Sample
Ovarian cancer cells
Sample name
OVCAR-3
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
1.09-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
42
Experiment ID
231
ISEV standards
✘
EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✘
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25332113
Organism
Homo sapiens
Experiment description
Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors
Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name
Transfusion
Publication year
2015
Sample
Platelets
Sample name
PL-Exs - Rep 1
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient
Flotation density
1.12-1.15 g/mL
Molecules identified in the study
Protein Lipids
Methods used in the study
Western blotting Mass spectrometry
43
Experiment ID
232
ISEV standards
✘
EV Biophysical techniques
✘
EV Cytosolic markers
✘
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25332113
Organism
Homo sapiens
Experiment description
Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors
Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name
Transfusion
Publication year
2015
Sample
Platelets
Sample name
PL-Exs - Rep 2
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient
Flotation density
1.12-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
44
Experiment ID
233
ISEV standards
✘
EV Biophysical techniques
✘
EV Cytosolic markers
✘
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25332113
Organism
Homo sapiens
Experiment description
Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors
Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name
Transfusion
Publication year
2015
Sample
Platelets
Sample name
PL-Exs - Rep 3
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient
Flotation density
1.12-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
45
Experiment ID
245
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
CD9|CD63|FLOT1
EV Membrane markers
✘
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
25519911
Organism
Homo sapiens
Experiment description
The ether lipid precursor hexadecylglycerol stimulates the release and changes the composition of exosomes derived from PC-3 cells
Authors
Phuyal S, Skotland T, Hessvik NP, Simolin H, Overbye A, Brech A, Parton RG, Ekroos K, Sandvig K, Llorente A.
Journal name
J Biol Chem
Publication year
2015
Sample
Prostate cancer cells
Sample name
PC-3 - Ethanol treated (control)
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Lipids Protein
Methods used in the study
Western blotting Mass spectrometry
46
Experiment ID
246
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
CD9|CD63|FLOT1
EV Membrane markers
✘
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
25519911
Organism
Homo sapiens
Experiment description
The ether lipid precursor hexadecylglycerol stimulates the release and changes the composition of exosomes derived from PC-3 cells
Authors
Phuyal S, Skotland T, Hessvik NP, Simolin H, Overbye A, Brech A, Parton RG, Ekroos K, Sandvig K, Llorente A.
Journal name
J Biol Chem
Publication year
2015
Sample
Prostate cancer cells
Sample name
PC-3 - sn-1-O-hexadecylglycerol treated
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Lipids
Methods used in the study
Western blotting Mass spectrometry
47
Experiment ID
247
ISEV standards
✘
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✘
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
25519911
Organism
Homo sapiens
Experiment description
The ether lipid precursor hexadecylglycerol stimulates the release and changes the composition of exosomes derived from PC-3 cells
Authors
Phuyal S, Skotland T, Hessvik NP, Simolin H, Overbye A, Brech A, Parton RG, Ekroos K, Sandvig K, Llorente A.
Journal name
J Biol Chem
Publication year
2015
Sample
Prostate cancer cells
Sample name
PC-3 - DL- palmitin treated
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Lipids
Methods used in the study
Western blotting Mass spectrometry
48
Experiment ID
250
ISEV standards
✔
EM
EV Biophysical techniques
✔
GAPDH|TSG101
EV Cytosolic markers
✔
CD63|CD81
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
25857301
Organism
Homo sapiens
Experiment description
Large oncosomes contain distinct protein cargo and represent a separate functional class of tumor-derived extracellular vesicles.
Authors
Minciacchi VR, You S, Spinelli C, Morley S, Zandian M, Aspuria PJ, Cavallini L, Ciardiello C, Reis Sobreiro M, Morello M, Kharmate G, Jang SC, Kim DK, Hosseini-Beheshti E, Tomlinson Guns E, Gleave M, Gho YS, Mathivanan S, Yang W, Freeman MR, Di Vizio D
Journal name
Oncotarget
Publication year
2015
Sample
Prostate cancer cells
Sample name
DU145 - expressing DIAPH3 hRNA
Isolation/purification methods
Differential centrifugation Ultracentrifugation OptiPrep density gradient
Flotation density
1.10 g/mL
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
49
Experiment ID
275
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|RAB5A
EV Cytosolic markers
✔
CD9|CD82|CD63|CD81
EV Membrane markers
✔
AIF
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting Mass spectrometry
PubMed ID
25844599
Organism
Homo sapiens
Experiment description
Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors
Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T.
Journal name
Oncotarget
Publication year
2015
Sample
Prostate cancer cells
Sample name
DU145 - Docetaxel sensitive
Isolation/purification methods
Filtration Ultracentrifugation Sucrose density gradient
Flotation density
1.12-1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry/Flow cytometry/Western blotting
50
Experiment ID
274
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|RAB5A
EV Cytosolic markers
✔
CD9|CD82|CD63|CD81
EV Membrane markers
✔
AIF
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting Mass spectrometry
PubMed ID
25844599
Organism
Homo sapiens
Experiment description
Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors
Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T.
Journal name
Oncotarget
Publication year
2015
Sample
Prostate cancer cells
Sample name
DU145 - Docetaxel resistant
Isolation/purification methods
Filtration Ultracentrifugation Sucrose density gradient
Flotation density
1.13-1.18 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry Flow cytometry Western blotting
51
Experiment ID
88
ISEV standards
✔
EM|IEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
CD63
EV Membrane markers
✔
HSPA5
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
20949044
Organism
Homo sapiens
Experiment description
Let-7 MicroRNA Family Is Selectively Secreted into the Extracellular Environment via Exosomes in a Metastatic Gastric Cancer Cell Line
Authors
Ohshima K, Inoue K, Fujiwara A, Hatakeyama K, Kanto K, Watanabe Y, Muramatsu K, Fukuda Y, Ogura S, Yamaguchi K, Mochizuki T.
Journal name
PLoS One
Publication year
2010
Sample
Stomach cancer cells
Sample name
AZ-P7a
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein miRNA
Methods used in the study
Immunoelectron Microscopy Western blotting RT-PCR Human miRNA Microarray Kit
52
Experiment ID
82
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✘
EV Membrane markers
✔
HSPA5
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
20949044
Organism
Homo sapiens
Experiment description
Let-7 MicroRNA Family Is Selectively Secreted into the Extracellular Environment via Exosomes in a Metastatic Gastric Cancer Cell Line
Authors
Ohshima K, Inoue K, Fujiwara A, Hatakeyama K, Kanto K, Watanabe Y, Muramatsu K, Fukuda Y, Ogura S, Yamaguchi K, Mochizuki T.
Journal name
PLoS One
Publication year
2010
Sample
Stomach cancer cells
Sample name
AZ-521
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein miRNA
Methods used in the study
Western blotting RT-PCR Human miRNA Microarray Kit
53
Experiment ID
115
ISEV standards
✘
EV Biophysical techniques
✘
EV Cytosolic markers
✔
CD63|CD81
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
21505438
Organism
Homo sapiens
Experiment description
Unidirectional transfer of microRNA-loaded exosomes from T cells to antigen-presenting cells.
Authors
Mittelbrunn M, Gutierrez-Vázquez C, Villarroya-Beltri C, González S, Sánchez-Cabo F, González MÁ, Bernad A, Sánchez-Madrid F.
Journal name
NATCOM
Publication year
2011
Sample
T cells
Sample name
J77
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein miRNA
Methods used in the study
Mass spectrometry Microarray
54
Experiment ID
34
ISEV standards
✔
EM|IEM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD63|MHCI|MHCII
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
19190083
Organism
Homo sapiens
Experiment description
Characterization of exosome-like vesicles released from human tracheobronchial ciliated epithelium: a possible role in innate defense.
Authors
Kesimer M, Scull M, Brighton B, Demaria G, Burns K, O'Neal W, Pickles RJ, Sheehan JK
Journal name
FASEB
Publication year
2009
Sample
Tracheobronchial cells
Sample name
Tracheobronchial epithelial cells
Isolation/purification methods
Differential centrifugation Filtration Sucrose density gradient
Flotation density
1.16 -1.18 g/mL
Molecules identified in the study
Protein mRNA
Methods used in the study
Mass spectrometry [QTOF] Western blotting Immunoelectron Microscopy FACS
55
Experiment ID
13
ISEV standards
✔
IEM
EV Biophysical techniques
✔
Alix|RAB4|RAB5B|RAB11|TSG101
EV Cytosolic markers
✔
CD9|AQP2|AQP1
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
15326289
Organism
Homo sapiens
Experiment description
Identification and proteomic profiling of exosomes in human urine.
Authors
Pisitkun T, Shen RF, Knepper MA
Journal name
PNAS
Publication year
2004
Sample
Urine
Sample name
Urine - Normal
Isolation/purification methods
Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [LCQ DECA XP] Western blotting
56
Experiment ID
63
ISEV standards
✘
EV Biophysical techniques
✘
EV Cytosolic markers
✔
AQP2
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
19056867
Organism
Homo sapiens
Experiment description
Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors
Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name
JASN
Publication year
2009
Sample
Urine
Sample name
Urine - Normal
Isolation/purification methods
Differential centrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [LTQ] Western blotting
57
Experiment ID
193
ISEV standards
✔
EM
EV Biophysical techniques
✘
EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✔
PHB
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
21595033
Organism
Homo sapiens
Experiment description
Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors
Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name
Proteomics
Publication year
2011
Sample
Urine
Sample name
Urine - Normal
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
58
Experiment ID
194
ISEV standards
✘
EV Biophysical techniques
✘
EV Cytosolic markers
✘
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
21595033
Organism
Homo sapiens
Experiment description
Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors
Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name
Proteomics
Publication year
2011
Sample
Urine
Sample name
Urine - Patients of basement membrane nephropathy
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
59
Experiment ID
195
ISEV standards
✘
EV Biophysical techniques
✘
EV Cytosolic markers
✘
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
21595033
Organism
Homo sapiens
Experiment description
Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors
Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name
Proteomics
Publication year
2011
Sample
Urine
Sample name
Urine - Patients of early IgA nephropathy
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
60
Experiment ID
196
ISEV standards
✔
EM
EV Biophysical techniques
✔
Alix|TSG101|HSP70
EV Cytosolic markers
✔
CD9
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
22418980
Organism
Homo sapiens
Experiment description
A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors
Raj DA, Fiume I, Capasso G, Pocsfalvi G.
Journal name
Kidney Int
Publication year
2012
Sample
Urine
Sample name
Urine - Normal high density
Isolation/purification methods
Differential centrifugation Sucrose cushion
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
61
Experiment ID
197
ISEV standards
✔
EM
EV Biophysical techniques
✔
Alix|TSG101|HSP70
EV Cytosolic markers
✔
CD9
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
22418980
Organism
Homo sapiens
Experiment description
A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors
Raj DA, Fiume I, Capasso G, Pocsfalvi G.
Journal name
Kidney Int
Publication year
2012
Sample
Urine
Sample name
Urine - Normal low density
Isolation/purification methods
Differential centrifugation Sucrose cushion
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
62
Experiment ID
238
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✘
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
25452312
Organism
Homo sapiens
Experiment description
N-linked (N-) glycoproteomics of urimary exosomes
Authors
Saraswat M, Joenvaara S, Musante L, Peltoniemi H4, Holthofer H, Renkonen R.
Journal name
Mol Cell Proteomics
Publication year
2014
Sample
Urine
Sample name
Urine - Normal
Isolation/purification methods
Differential centrifugation Filtration Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
Protein-protein interactions for TSG101
Protein Interactor
ExoCarta ID
Identification method
PubMed
Species
1
PPFIBP2
8495
Two-hybrid
Homo sapiens
2
CEP55
55165
Two-hybrid
Homo sapiens
3
VPS37C
55048
Reconstituted Complex
Homo sapiens
Two-hybrid
Homo sapiens
4
HGS
9146
Affinity Capture-Western
Homo sapiens
Two-hybrid
Homo sapiens
Affinity Capture-MS
Homo sapiens
Two-hybrid
Homo sapiens
Affinity Capture-Western
Homo sapiens
5
VPS28
51160
Two-hybrid
Homo sapiens
Affinity Capture-MS
Homo sapiens
Two-hybrid
Homo sapiens
Reconstituted Complex
Homo sapiens
6
GGA3
23163
Two-hybrid
Homo sapiens
7
KRT15
3866
Two-hybrid
Homo sapiens
8
AR
367
Two-hybrid
Homo sapiens
9
TSG101
7251
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
10
KIF5A
3798
Two-hybrid
Homo sapiens
11
NR3C1
2908
Affinity Capture-MS
Homo sapiens
Two-hybrid
Homo sapiens
12
THRB
7068
Two-hybrid
Homo sapiens
13
ITSN1
6453
Two-hybrid
Homo sapiens
14
FLJ32642
Two-hybrid
Homo sapiens
Reconstituted Complex
Homo sapiens
Two-hybrid
Homo sapiens
15
CDKN1A
Affinity Capture-MS
Homo sapiens
Affinity Capture-Western
Homo sapiens
Two-hybrid
Homo sapiens
16
DMAP1
55929
Two-hybrid
Homo sapiens
17
MDM2
Invivo
Homo sapiens
18
LRSAM1
90678
Affinity Capture-Western
Homo sapiens
Two-hybrid
Homo sapiens
Two-hybrid
Homo sapiens
Reconstituted Complex
Homo sapiens
19
TP53
Invivo
Homo sapiens
Affinity Capture-MS
Homo sapiens
20
VPS37B
79720
Reconstituted Complex
Homo sapiens
Two-hybrid
Homo sapiens
Affinity Capture-Western
Homo sapiens
Affinity Capture-MS
Homo sapiens
Two-hybrid
Homo sapiens
21
EP300
2033
Affinity Capture-Western
Homo sapiens
Reconstituted Complex
Homo sapiens
View the network
image/svg+xml
Pathways in which TSG101 is involved