Gene description for HECTD3
Gene name HECT domain containing E3 ubiquitin protein ligase 3
Gene symbol HECTD3
Other names/aliases -
Species Homo sapiens
 Database cross references - HECTD3
ExoCarta ExoCarta_79654
Vesiclepedia VP_79654
Entrez Gene 79654
HGNC 26117
UniProt Q5T447  
 HECTD3 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for HECTD3
Molecular Function
    ubiquitin-protein transferase activity GO:0004842 IBA
    ubiquitin-protein transferase activity GO:0004842 IDA
    ubiquitin-protein transferase activity GO:0004842 ISS
    protein binding GO:0005515 IPI
    syntaxin binding GO:0019905 IEA
Biological Process
    protein ubiquitination GO:0016567 IEA
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IBA
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IDA
Subcellular Localization
    perinuclear region of cytoplasm GO:0048471 ISS
 Experiment description of studies that identified HECTD3 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for HECTD3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CLEC3A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 RPS4Y1 6192
Affinity Capture-MS Homo sapiens
3 MRPL30  
Affinity Capture-MS Homo sapiens
4 UBXN10  
Affinity Capture-MS Homo sapiens
5 BRCA1 672
Two-hybrid Homo sapiens
6 LAD1 3898
Affinity Capture-MS Homo sapiens
7 CCT4 10575
Affinity Capture-MS Homo sapiens
8 CCT6B 10693
Affinity Capture-MS Homo sapiens
9 PCBP3 54039
Affinity Capture-MS Homo sapiens
10 RSPO1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 PPL 5493
Two-hybrid Homo sapiens
12 SULF2 55959
Affinity Capture-MS Homo sapiens
13 TRIOBP 11078
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
14 STX8 9482
Affinity Capture-Western Homo sapiens
15 MRPS24 64951
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 WNT9B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 SERPINA5 5104
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 BAGE2  
Affinity Capture-MS Homo sapiens
19 FTSJ2 29960
Affinity Capture-MS Homo sapiens
20 MRPS26 64949
Affinity Capture-MS Homo sapiens
21 COX7B2  
Affinity Capture-MS Homo sapiens
22 MRPS7 51081
Affinity Capture-MS Homo sapiens
23 CCDC130  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 MAVS 57506
Affinity Capture-MS Homo sapiens
25 LRRC17 10234
Affinity Capture-MS Homo sapiens
26 VWA8 23078
Affinity Capture-MS Homo sapiens
27 C9orf163  
Affinity Capture-MS Homo sapiens
28 CCT2 10576
Affinity Capture-MS Homo sapiens
29 AURKA 6790
Affinity Capture-MS Homo sapiens
30 ZDHHC17 23390
Two-hybrid Homo sapiens
31 TCP1 6950
Affinity Capture-MS Homo sapiens
32 IRAK1  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
33 MRPS34 65993
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 CEP192 55125
Affinity Capture-MS Homo sapiens
35 BAG6 7917
Affinity Capture-MS Homo sapiens
36 NTRK1 4914
Affinity Capture-MS Homo sapiens
37 EMILIN3  
Affinity Capture-MS Homo sapiens
38 MALT1 10892
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
39 FEZF1  
Affinity Capture-MS Homo sapiens
40 PATE1  
Affinity Capture-MS Homo sapiens
41 GSG2  
Affinity Capture-MS Homo sapiens
42 COPS7B 64708
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 TTC25  
Affinity Capture-MS Homo sapiens
44 DKK2  
Affinity Capture-MS Homo sapiens
45 UBXN6 80700
Affinity Capture-MS Homo sapiens
46 C16orf72 29035
Affinity Capture-MS Homo sapiens
47 ZRANB1 54764
Affinity Capture-MS Homo sapiens
48 MRM1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 HLA-DPB1 3115
Affinity Capture-MS Homo sapiens
50 ZBBX  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 TRMT10C 54931
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 CPA6  
Affinity Capture-MS Homo sapiens
53 CCT6A 908
Affinity Capture-MS Homo sapiens
54 GAST  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 SPINK14  
Affinity Capture-MS Homo sapiens
56 CUL3 8452
Affinity Capture-MS Homo sapiens
57 NR3C1 2908
Proximity Label-MS Homo sapiens
58 RPL3 6122
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 PRR27 401137
Affinity Capture-MS Homo sapiens
60 LYZL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 TRAF3 7187
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
62 FOSL2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 UBE2D2 7322
Reconstituted Complex Homo sapiens
64 GADD45GIP1  
Affinity Capture-MS Homo sapiens
65 NCAPH 23397
Co-fractionation Homo sapiens
66 CNR2  
Affinity Capture-MS Homo sapiens
67 CHRDL1 91851
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 PLA2G2D  
Affinity Capture-MS Homo sapiens
69 SLC7A11 23657
Affinity Capture-MS Homo sapiens
70 CCNG1  
Affinity Capture-MS Homo sapiens
71 POGLUT1 56983
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 WFDC10A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
73 ANGPT2 285
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
74 SCCPDH 51097
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
75 HUWE1 10075
Affinity Capture-MS Homo sapiens
76 NKAP 79576
Affinity Capture-MS Homo sapiens
77 LYZL2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
78 DNAJC28  
Affinity Capture-MS Homo sapiens
79 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
80 FAM81A  
Affinity Capture-MS Homo sapiens
81 RBFA  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
82 TP53 7157
Two-hybrid Homo sapiens
83 PTRF 284119
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
84 ZZEF1 23140
Co-fractionation Homo sapiens
85 CASP8 841
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
86 RPS4X 6191
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
87 HSP90AA1 3320
Affinity Capture-Luminescence Homo sapiens
88 OVOL2  
Affinity Capture-MS Homo sapiens
89 CCT7 10574
Affinity Capture-MS Homo sapiens
90 FST 10468
Affinity Capture-MS Homo sapiens
91 ZNF317 57693
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
92 EGFL6 25975
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
93 PARD6B 84612
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
94 TEFM  
Affinity Capture-MS Homo sapiens
95 PRDX1 5052
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
96 BRD1 23774
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
97 EBI3 10148
Affinity Capture-MS Homo sapiens
98 MYC  
Dosage Lethality Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
99 GAS2L1 10634
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
100 TIMP3 7078
Affinity Capture-MS Homo sapiens
101 MRPS9 64965
Affinity Capture-MS Homo sapiens
102 GREM2 64388
Affinity Capture-MS Homo sapiens
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